Streptococcus satellite phage Javan175

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZK28|A0A4D5ZK28_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan175 OX=2558555 GN=JavanS175_0011 PE=4 SV=1
MM1 pKa = 7.55FDD3 pKa = 5.16DD4 pKa = 4.03VMKK7 pKa = 10.81KK8 pKa = 9.86IADD11 pKa = 3.81FLEE14 pKa = 4.26NTPKK18 pKa = 10.66DD19 pKa = 3.18IYY21 pKa = 10.93EE22 pKa = 3.83FSIWLEE28 pKa = 3.89DD29 pKa = 3.87TLVDD33 pKa = 5.57DD34 pKa = 4.7YY35 pKa = 12.0DD36 pKa = 5.06AMAAEE41 pKa = 4.66QPRR44 pKa = 11.84ATYY47 pKa = 11.0LLGQEE52 pKa = 4.61VPDD55 pKa = 3.58ICASAEE61 pKa = 3.86PGMKK65 pKa = 9.96AEE67 pKa = 4.44EE68 pKa = 3.9VAEE71 pKa = 4.81FKK73 pKa = 10.77RR74 pKa = 11.84QLKK77 pKa = 9.99VEE79 pKa = 4.0YY80 pKa = 10.32DD81 pKa = 3.21KK82 pKa = 11.84ALLFVVV88 pKa = 4.42

Molecular weight: 10.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZG39|A0A4D5ZG39_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan175 OX=2558555 GN=JavanS175_0009 PE=4 SV=1
MM1 pKa = 7.94KK2 pKa = 9.36ITTYY6 pKa = 8.91TKK8 pKa = 10.32KK9 pKa = 10.6DD10 pKa = 2.96GSTVYY15 pKa = 9.14RR16 pKa = 11.84TSVFLGVDD24 pKa = 3.02QVTGKK29 pKa = 10.31KK30 pKa = 10.63VNTTVSARR38 pKa = 11.84TKK40 pKa = 10.72RR41 pKa = 11.84EE42 pKa = 3.44LDD44 pKa = 2.99QKK46 pKa = 11.04ARR48 pKa = 11.84RR49 pKa = 11.84KK50 pKa = 9.98ILDD53 pKa = 3.38FEE55 pKa = 4.62KK56 pKa = 10.87AGSTVHH62 pKa = 6.65KK63 pKa = 10.23AVAVKK68 pKa = 9.28TYY70 pKa = 10.91QEE72 pKa = 4.23LAEE75 pKa = 4.63LWWEE79 pKa = 4.55SYY81 pKa = 7.63QHH83 pKa = 5.16TVKK86 pKa = 11.0SNTQDD91 pKa = 3.18GVRR94 pKa = 11.84KK95 pKa = 9.67LLNNHH100 pKa = 6.74ILPAFGTYY108 pKa = 10.31RR109 pKa = 11.84LDD111 pKa = 3.55KK112 pKa = 9.79LTPPLIQGIINKK124 pKa = 8.96IAKK127 pKa = 9.08AANEE131 pKa = 4.29GQPGAYY137 pKa = 9.55LHH139 pKa = 6.38YY140 pKa = 10.92DD141 pKa = 3.94KK142 pKa = 11.13IHH144 pKa = 6.25GLNKK148 pKa = 10.25RR149 pKa = 11.84ILQYY153 pKa = 11.19GVILQALPYY162 pKa = 9.32NPARR166 pKa = 11.84EE167 pKa = 4.25VILPRR172 pKa = 11.84NTAKK176 pKa = 10.72ADD178 pKa = 3.9RR179 pKa = 11.84QTVKK183 pKa = 10.04HH184 pKa = 5.91WEE186 pKa = 3.87NDD188 pKa = 3.33QLKK191 pKa = 10.96AFLDD195 pKa = 3.88YY196 pKa = 11.23LGNLDD201 pKa = 4.03LNRR204 pKa = 11.84YY205 pKa = 8.56RR206 pKa = 11.84YY207 pKa = 9.85LYY209 pKa = 10.6EE210 pKa = 3.57VTLYY214 pKa = 11.0KK215 pKa = 10.51FLLATGCRR223 pKa = 11.84INEE226 pKa = 4.03ALALDD231 pKa = 3.83WSDD234 pKa = 3.09IDD236 pKa = 4.75LEE238 pKa = 4.21ARR240 pKa = 11.84TVNVTKK246 pKa = 10.21TLDD249 pKa = 3.36YY250 pKa = 9.91KK251 pKa = 11.07QEE253 pKa = 4.08VNSPKK258 pKa = 10.51SKK260 pKa = 10.32ASNRR264 pKa = 11.84IIDD267 pKa = 3.82IDD269 pKa = 3.76QATCAMLHH277 pKa = 5.28QYY279 pKa = 10.87KK280 pKa = 10.21LRR282 pKa = 11.84QVQEE286 pKa = 3.49AWQLGRR292 pKa = 11.84TEE294 pKa = 4.32TVVFSDD300 pKa = 6.06FIHH303 pKa = 7.3AYY305 pKa = 8.66PNNRR309 pKa = 11.84TLQTRR314 pKa = 11.84LRR316 pKa = 11.84THH318 pKa = 7.0FKK320 pKa = 10.22RR321 pKa = 11.84AGMPNIGFHH330 pKa = 6.14GFRR333 pKa = 11.84HH334 pKa = 4.87THH336 pKa = 6.58ASLLLNSGIPYY347 pKa = 10.0KK348 pKa = 10.34EE349 pKa = 3.8LQHH352 pKa = 6.73RR353 pKa = 11.84LGHH356 pKa = 5.68STLSMTMDD364 pKa = 3.58IYY366 pKa = 11.69SHH368 pKa = 6.89LSKK371 pKa = 10.82EE372 pKa = 4.24KK373 pKa = 10.55AKK375 pKa = 10.69SAVSFFEE382 pKa = 4.28KK383 pKa = 10.47ALKK386 pKa = 10.54SII388 pKa = 4.31

Molecular weight: 44.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

2917

40

521

145.8

16.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.411 ± 0.382

0.754 ± 0.19

6.685 ± 0.37

8.125 ± 0.79

3.908 ± 0.576

4.354 ± 0.434

1.714 ± 0.337

5.348 ± 0.422

8.982 ± 0.628

11.45 ± 0.616

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.125 ± 0.255

4.662 ± 0.458

3.325 ± 0.509

5.279 ± 0.362

5.931 ± 0.583

4.285 ± 0.374

5.965 ± 0.398

5.348 ± 0.437

1.166 ± 0.127

4.182 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski