Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanocellales; Methanocellaceae; Methanocella; Methanocella arvoryzae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3071 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0W6E3|Q0W6E3_METAR Glutamine synthetase OS=Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50) OX=351160 GN=glnA-1 PE=3 SV=1
MM1 pKa = 7.29IAASALFAAPALADD15 pKa = 4.06FPKK18 pKa = 10.82SSDD21 pKa = 3.21IQFSQSNYY29 pKa = 10.56DD30 pKa = 3.43VYY32 pKa = 11.53EE33 pKa = 4.0LANGNDD39 pKa = 3.19VTVRR43 pKa = 11.84ITVTINDD50 pKa = 4.58LDD52 pKa = 4.87LDD54 pKa = 4.0DD55 pKa = 5.04DD56 pKa = 3.66EE57 pKa = 6.23RR58 pKa = 11.84YY59 pKa = 9.45RR60 pKa = 11.84VHH62 pKa = 7.22INTDD66 pKa = 2.48QRR68 pKa = 11.84TALRR72 pKa = 11.84DD73 pKa = 3.24EE74 pKa = 4.44DD75 pKa = 5.3RR76 pKa = 11.84YY77 pKa = 10.35GQRR80 pKa = 11.84DD81 pKa = 3.67YY82 pKa = 11.73VPYY85 pKa = 9.56WEE87 pKa = 4.42HH88 pKa = 7.54DD89 pKa = 3.44YY90 pKa = 11.05RR91 pKa = 11.84YY92 pKa = 10.68LEE94 pKa = 4.19FTSADD99 pKa = 3.3NGAIAQKK106 pKa = 10.5HH107 pKa = 5.13FDD109 pKa = 3.77VTVHH113 pKa = 6.41SNTVDD118 pKa = 3.62DD119 pKa = 4.72AATEE123 pKa = 4.2YY124 pKa = 10.7FVVEE128 pKa = 3.69MRR130 pKa = 11.84GYY132 pKa = 8.32WKK134 pKa = 10.47GYY136 pKa = 6.81KK137 pKa = 8.77TLIGWIYY144 pKa = 10.08PDD146 pKa = 3.51YY147 pKa = 11.52DD148 pKa = 3.35SFKK151 pKa = 10.68NIKK154 pKa = 9.52RR155 pKa = 11.84SNVTIYY161 pKa = 10.89DD162 pKa = 3.94ADD164 pKa = 3.74TTAPITTATASGTSGANGWYY184 pKa = 8.97TSIVQVTLTASDD196 pKa = 3.89EE197 pKa = 4.37DD198 pKa = 3.84SGVANTYY205 pKa = 7.74YY206 pKa = 10.47TIDD209 pKa = 3.53GGAQQTYY216 pKa = 9.6SAPFTVTDD224 pKa = 3.96GTHH227 pKa = 7.09TIQYY231 pKa = 9.01WSVDD235 pKa = 3.07KK236 pKa = 11.41AGNTEE241 pKa = 4.05TAQTLNLNVDD251 pKa = 3.92TKK253 pKa = 11.52APVITSDD260 pKa = 3.55RR261 pKa = 11.84TPANANGWNNGDD273 pKa = 3.52VTVSFTATDD282 pKa = 3.91DD283 pKa = 3.73GSGISGSASYY293 pKa = 10.78SQTLTGEE300 pKa = 4.5GSSQSVEE307 pKa = 3.73WSVTDD312 pKa = 4.7LAGNTANATVSNINIDD328 pKa = 3.28KK329 pKa = 7.95TAPSITGAVDD339 pKa = 3.43RR340 pKa = 11.84DD341 pKa = 3.6ANANGWYY348 pKa = 9.92NADD351 pKa = 3.38VTVTFTAGADD361 pKa = 3.5LSGIASITDD370 pKa = 3.34PVVLGEE376 pKa = 4.24GAGQSVTGTVVDD388 pKa = 4.23LAGNSASTTVSGINVDD404 pKa = 2.96KK405 pKa = 9.92TAPVLVSSGPTTSPNINGWYY425 pKa = 10.22NGDD428 pKa = 3.39VTVNFVGSDD437 pKa = 3.37ALSGISGSTSADD449 pKa = 3.0VVVSTEE455 pKa = 4.01GADD458 pKa = 3.5EE459 pKa = 4.15VASYY463 pKa = 10.06TFTDD467 pKa = 3.37KK468 pKa = 11.26AGNSATFYY476 pKa = 9.65VTGIKK481 pKa = 9.82LDD483 pKa = 3.37KK484 pKa = 8.64TAPVITTARR493 pKa = 11.84TPANANGWNNGPVTVSFTATDD514 pKa = 3.4AGSGIDD520 pKa = 3.86GDD522 pKa = 3.85ATYY525 pKa = 10.72EE526 pKa = 3.96EE527 pKa = 5.05TVAGDD532 pKa = 4.21GEE534 pKa = 4.22DD535 pKa = 4.75QEE537 pKa = 5.7ASWSVSDD544 pKa = 4.72LAGNSASATVGGINIDD560 pKa = 3.56TAAPTTTDD568 pKa = 4.17DD569 pKa = 4.11LTGTLGNDD577 pKa = 2.81GWFLTDD583 pKa = 2.77VHH585 pKa = 5.85VTLSATDD592 pKa = 3.77GAAFQAVSGSGLLLLAAGSSDD613 pKa = 3.42EE614 pKa = 4.23GSSGIAEE621 pKa = 3.79IWYY624 pKa = 8.46TLDD627 pKa = 2.89GSAPIAYY634 pKa = 8.44PDD636 pKa = 3.51GGFDD640 pKa = 3.32VTGNGQHH647 pKa = 6.81VITFWSVDD655 pKa = 3.07AAGNAEE661 pKa = 4.59EE662 pKa = 4.85PNTDD666 pKa = 2.92NFKK669 pKa = 10.19IDD671 pKa = 3.17KK672 pKa = 9.05DD673 pKa = 3.94APVTTFSVTGAEE685 pKa = 4.32VTLDD689 pKa = 3.32AADD692 pKa = 4.71NIDD695 pKa = 3.62GSGVAATYY703 pKa = 7.58YY704 pKa = 9.65TLNGGLTQTYY714 pKa = 9.35IGSFPLPDD722 pKa = 3.21GTYY725 pKa = 9.99TIEE728 pKa = 4.16FWSEE732 pKa = 3.56DD733 pKa = 3.23VAGNVEE739 pKa = 4.04LHH741 pKa = 6.61GDD743 pKa = 3.87GLNTEE748 pKa = 4.39TFTVEE753 pKa = 4.12TVQSYY758 pKa = 8.46TIHH761 pKa = 7.49LLTGWNLVSSPLIIEE776 pKa = 4.34PMRR779 pKa = 11.84ASDD782 pKa = 3.44IVGNGITMVAKK793 pKa = 9.99YY794 pKa = 10.63NKK796 pKa = 8.83DD797 pKa = 2.97TGEE800 pKa = 3.82FTIYY804 pKa = 10.81SMEE807 pKa = 4.1INQPGDD813 pKa = 3.31PEE815 pKa = 4.84DD816 pKa = 3.94FVVTNDD822 pKa = 2.81VGYY825 pKa = 10.97YY826 pKa = 9.63FAASQDD832 pKa = 2.92MDD834 pKa = 3.75YY835 pKa = 10.77TFVGVYY841 pKa = 10.53APVPYY846 pKa = 10.49SIDD849 pKa = 3.45VPGDD853 pKa = 3.63NRR855 pKa = 11.84WDD857 pKa = 3.35IYY859 pKa = 11.32GWTSLQSSTAYY870 pKa = 9.7EE871 pKa = 4.37VASLMDD877 pKa = 4.42GMQMIAVYY885 pKa = 10.63NHH887 pKa = 6.53DD888 pKa = 3.7TGGFTIFSEE897 pKa = 4.92EE898 pKa = 4.04INTVQDD904 pKa = 3.15AEE906 pKa = 4.31NFVMGPGVGYY916 pKa = 9.98FITSDD921 pKa = 3.25TTTTLAYY928 pKa = 8.36EE929 pKa = 4.5VPVV932 pKa = 3.52

Molecular weight: 98.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0W584|Q0W584_METAR Uncharacterized protein OS=Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50) OX=351160 GN=RCIX1151 PE=4 SV=1
MM1 pKa = 7.4EE2 pKa = 5.84CSNKK6 pKa = 9.85CLMLACVVLALAVLTIGPAAAQLVQTGGFTRR37 pKa = 11.84TYY39 pKa = 9.0QKK41 pKa = 10.82SVAGGTFVPPQAEE54 pKa = 4.14VSEE57 pKa = 4.47TFYY60 pKa = 10.78QYY62 pKa = 11.02PGISAWGRR70 pKa = 11.84IPGRR74 pKa = 11.84WLCLRR79 pKa = 11.84LWRR82 pKa = 11.84RR83 pKa = 11.84PDD85 pKa = 3.38SVRR88 pKa = 11.84LRR90 pKa = 11.84AIRR93 pKa = 11.84RR94 pKa = 11.84LHH96 pKa = 5.56VPLRR100 pKa = 11.84NPVGKK105 pKa = 10.02KK106 pKa = 9.08ASEE109 pKa = 4.19WVSGG113 pKa = 3.63

Molecular weight: 12.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3071

0

3071

889879

44

1827

289.8

31.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.715 ± 0.06

1.297 ± 0.023

5.57 ± 0.037

6.184 ± 0.058

3.667 ± 0.033

7.808 ± 0.046

1.759 ± 0.019

7.183 ± 0.045

5.565 ± 0.05

9.24 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.967 ± 0.024

3.43 ± 0.034

4.562 ± 0.034

2.709 ± 0.022

5.446 ± 0.04

6.157 ± 0.038

5.623 ± 0.047

7.586 ± 0.043

0.943 ± 0.017

3.59 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski