Methylobacter tundripaludum (strain ATCC BAA-1195 / DSM 17260 / SV96)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylobacter; Methylobacter tundripaludum

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4194 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G3J233|G3J233_METTV Putative signal transduction protein OS=Methylobacter tundripaludum (strain ATCC BAA-1195 / DSM 17260 / SV96) OX=697282 GN=Mettu_2904 PE=4 SV=1
MM1 pKa = 7.15TVDD4 pKa = 4.64FNHH7 pKa = 6.9LKK9 pKa = 9.74HH10 pKa = 6.9FSMTYY15 pKa = 10.25VFMDD19 pKa = 4.03EE20 pKa = 4.27EE21 pKa = 6.34DD22 pKa = 3.41IACEE26 pKa = 4.04YY27 pKa = 10.06EE28 pKa = 4.07QTEE31 pKa = 4.77PIPVVAADD39 pKa = 3.88GKK41 pKa = 10.97SISFNLRR48 pKa = 11.84NIDD51 pKa = 3.45QSEE54 pKa = 4.19DD55 pKa = 3.32KK56 pKa = 10.81DD57 pKa = 4.08VYY59 pKa = 11.17SVVLVKK65 pKa = 10.94EE66 pKa = 4.52GDD68 pKa = 3.43DD69 pKa = 3.42EE70 pKa = 5.69FYY72 pKa = 10.75IKK74 pKa = 10.42SDD76 pKa = 3.75YY77 pKa = 10.82FDD79 pKa = 5.35DD80 pKa = 4.32AAEE83 pKa = 4.64PYY85 pKa = 10.53PMDD88 pKa = 4.75VEE90 pKa = 5.1ISDD93 pKa = 5.13DD94 pKa = 3.64DD95 pKa = 4.16VKK97 pKa = 11.22FILEE101 pKa = 4.34GEE103 pKa = 4.27DD104 pKa = 3.27EE105 pKa = 4.45VMYY108 pKa = 10.69LYY110 pKa = 11.23GFFEE114 pKa = 4.35

Molecular weight: 13.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G3IVX0|G3IVX0_METTV Two component sigma54 specific transcriptional regulator Fis family OS=Methylobacter tundripaludum (strain ATCC BAA-1195 / DSM 17260 / SV96) OX=697282 GN=Mettu_1815 PE=4 SV=1
MM1 pKa = 7.13ATALSANLCIVSWTYY16 pKa = 11.13PFLKK20 pKa = 10.11HH21 pKa = 5.96YY22 pKa = 10.81VLGDD26 pKa = 3.5SCRR29 pKa = 11.84AEE31 pKa = 4.13CGLRR35 pKa = 11.84LAKK38 pKa = 10.17ARR40 pKa = 11.84HH41 pKa = 5.69RR42 pKa = 11.84KK43 pKa = 9.59AGLCLEE49 pKa = 4.39YY50 pKa = 10.98ARR52 pKa = 11.84VIASLLNWGVSSALQSCPCQPYY74 pKa = 10.25SRR76 pKa = 11.84SSTCFTLSRR85 pKa = 11.84CMGSSLAFGIKK96 pKa = 9.19IQNPNNLNHH105 pKa = 5.63YY106 pKa = 9.32HH107 pKa = 5.83SPVWLLRR114 pKa = 11.84TKK116 pKa = 10.4PLALRR121 pKa = 11.84QEE123 pKa = 4.03QSLANSLRR131 pKa = 11.84DD132 pKa = 3.45PRR134 pKa = 11.84CPLKK138 pKa = 10.83NKK140 pKa = 9.37PPRR143 pKa = 11.84NIWRR147 pKa = 11.84RR148 pKa = 11.84RR149 pKa = 11.84LMNKK153 pKa = 7.19SQCIIKK159 pKa = 10.25PPLTLSSLSMPRR171 pKa = 11.84QQRR174 pKa = 11.84KK175 pKa = 7.1PHH177 pKa = 4.68QQ178 pKa = 3.7

Molecular weight: 20.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4194

0

4194

1362415

24

7729

324.8

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.36 ± 0.05

1.078 ± 0.015

5.574 ± 0.033

5.974 ± 0.043

3.997 ± 0.024

7.058 ± 0.045

2.321 ± 0.02

6.424 ± 0.04

5.034 ± 0.047

10.661 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.023

4.07 ± 0.035

4.305 ± 0.032

4.403 ± 0.028

5.051 ± 0.035

6.08 ± 0.036

5.425 ± 0.058

6.639 ± 0.031

1.274 ± 0.016

2.891 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski