Geobacillus phage GBSV1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Svunavirus; Geobacillus virus GBSV1

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0H273|Q0H273_9CAUD Putative recombination protein U OS=Geobacillus phage GBSV1 OX=365048 PE=3 SV=2
MM1 pKa = 7.59LVRR4 pKa = 11.84FSLLYY9 pKa = 9.85KK10 pKa = 10.2SGYY13 pKa = 9.27EE14 pKa = 3.8DD15 pKa = 4.64VIEE18 pKa = 4.23QVCLPSEE25 pKa = 4.24VGNIIQTIRR34 pKa = 11.84GSFYY38 pKa = 10.24EE39 pKa = 4.68DD40 pKa = 3.15SPGYY44 pKa = 10.27ISFGDD49 pKa = 3.98EE50 pKa = 3.8NLKK53 pKa = 10.86GYY55 pKa = 9.82IINVQEE61 pKa = 4.09VARR64 pKa = 11.84VKK66 pKa = 10.39MEE68 pKa = 3.72ILEE71 pKa = 4.77GGDD74 pKa = 3.43SS75 pKa = 3.4

Molecular weight: 8.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0H252|Q0H252_9CAUD HTH_3 OS=Geobacillus phage GBSV1 OX=365048 PE=4 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84QGSQRR7 pKa = 11.84VLRR10 pKa = 11.84SEE12 pKa = 3.58ATRR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 9.85EE18 pKa = 3.57GGARR22 pKa = 11.84GGRR25 pKa = 11.84KK26 pKa = 8.38PQTKK30 pKa = 9.48EE31 pKa = 3.63VIGVLFRR38 pKa = 11.84RR39 pKa = 11.84QPEE42 pKa = 4.37TIPFRR47 pKa = 11.84DD48 pKa = 5.12FIYY51 pKa = 10.25GKK53 pKa = 8.26QTAGKK58 pKa = 8.35RR59 pKa = 11.84AKK61 pKa = 9.78TGVVVPALFPVITPEE76 pKa = 3.83NLFPIHH82 pKa = 7.06DD83 pKa = 4.31HH84 pKa = 7.12DD85 pKa = 5.55FSLLMIGVGSITLAAFLEE103 pKa = 4.68RR104 pKa = 11.84GLVMIGMTDD113 pKa = 3.2MAEE116 pKa = 4.12KK117 pKa = 10.63VAGCGRR123 pKa = 11.84IVFPIAVYY131 pKa = 10.14GAVLWLFFSLGGLL144 pKa = 3.63

Molecular weight: 15.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

10658

44

955

197.4

22.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.727 ± 0.468

0.807 ± 0.145

5.733 ± 0.186

7.9 ± 0.387

4.391 ± 0.249

6.042 ± 0.324

1.614 ± 0.221

7.206 ± 0.28

8.013 ± 0.419

8.594 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.173

4.513 ± 0.26

3.5 ± 0.155

3.875 ± 0.255

5.817 ± 0.347

5.236 ± 0.329

5.198 ± 0.337

6.962 ± 0.322

1.098 ± 0.103

4.119 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski