Amino acid dipepetide frequency for Geobacillus phage GBSV1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.161AlaAla: 5.161 ± 0.816
0.282AlaCys: 0.282 ± 0.151
4.316AlaAsp: 4.316 ± 0.698
4.973AlaGlu: 4.973 ± 0.571
4.129AlaPhe: 4.129 ± 0.687
3.66AlaGly: 3.66 ± 0.813
0.751AlaHis: 0.751 ± 0.339
5.442AlaIle: 5.442 ± 1.425
5.067AlaLys: 5.067 ± 0.733
5.912AlaLeu: 5.912 ± 0.92
1.971AlaMet: 1.971 ± 0.385
3.19AlaAsn: 3.19 ± 0.494
1.314AlaPro: 1.314 ± 0.328
2.721AlaGln: 2.721 ± 0.74
2.158AlaArg: 2.158 ± 0.452
2.721AlaSer: 2.721 ± 0.57
3.941AlaThr: 3.941 ± 0.573
5.724AlaVal: 5.724 ± 1.018
0.563AlaTrp: 0.563 ± 0.34
3.284AlaTyr: 3.284 ± 0.54
0.0AlaXaa: 0.0 ± 0.0
Cys
0.282CysAla: 0.282 ± 0.148
0.0CysCys: 0.0 ± 0.0
0.282CysAsp: 0.282 ± 0.175
0.563CysGlu: 0.563 ± 0.265
0.282CysPhe: 0.282 ± 0.167
0.657CysGly: 0.657 ± 0.257
0.094CysHis: 0.094 ± 0.102
0.375CysIle: 0.375 ± 0.189
1.126CysLys: 1.126 ± 0.366
0.845CysLeu: 0.845 ± 0.266
0.094CysMet: 0.094 ± 0.088
0.188CysAsn: 0.188 ± 0.132
0.375CysPro: 0.375 ± 0.198
0.469CysGln: 0.469 ± 0.211
0.657CysArg: 0.657 ± 0.277
0.563CysSer: 0.563 ± 0.262
0.188CysThr: 0.188 ± 0.123
0.469CysVal: 0.469 ± 0.213
0.0CysTrp: 0.0 ± 0.0
0.563CysTyr: 0.563 ± 0.255
0.0CysXaa: 0.0 ± 0.0
Asp
3.941AspAla: 3.941 ± 0.629
0.657AspCys: 0.657 ± 0.33
3.753AspAsp: 3.753 ± 0.691
6.381AspGlu: 6.381 ± 0.667
2.534AspPhe: 2.534 ± 0.54
4.41AspGly: 4.41 ± 0.594
0.845AspHis: 0.845 ± 0.272
4.692AspIle: 4.692 ± 0.748
2.909AspLys: 2.909 ± 0.458
4.41AspLeu: 4.41 ± 0.605
1.877AspMet: 1.877 ± 0.42
1.877AspAsn: 1.877 ± 0.339
1.126AspPro: 1.126 ± 0.258
2.627AspGln: 2.627 ± 0.426
3.19AspArg: 3.19 ± 0.749
2.627AspSer: 2.627 ± 0.709
2.815AspThr: 2.815 ± 0.515
4.129AspVal: 4.129 ± 0.538
0.657AspTrp: 0.657 ± 0.239
1.877AspTyr: 1.877 ± 0.379
0.0AspXaa: 0.0 ± 0.0
Glu
5.349GluAla: 5.349 ± 0.77
0.563GluCys: 0.563 ± 0.244
3.66GluAsp: 3.66 ± 0.511
5.255GluGlu: 5.255 ± 0.804
4.692GluPhe: 4.692 ± 0.685
3.284GluGly: 3.284 ± 0.652
0.938GluHis: 0.938 ± 0.278
6.099GluIle: 6.099 ± 0.767
6.662GluLys: 6.662 ± 0.764
9.571GluLeu: 9.571 ± 1.202
2.064GluMet: 2.064 ± 0.397
3.19GluAsn: 3.19 ± 0.684
2.064GluPro: 2.064 ± 0.459
4.129GluGln: 4.129 ± 0.682
5.161GluArg: 5.161 ± 0.68
2.534GluSer: 2.534 ± 0.459
3.472GluThr: 3.472 ± 0.584
5.536GluVal: 5.536 ± 0.729
1.126GluTrp: 1.126 ± 0.36
3.66GluTyr: 3.66 ± 0.616
0.0GluXaa: 0.0 ± 0.0
Phe
2.064PheAla: 2.064 ± 0.386
0.375PheCys: 0.375 ± 0.173
3.097PheAsp: 3.097 ± 0.639
3.566PheGlu: 3.566 ± 0.511
1.314PhePhe: 1.314 ± 0.377
2.534PheGly: 2.534 ± 0.445
0.375PheHis: 0.375 ± 0.207
3.66PheIle: 3.66 ± 0.57
3.003PheLys: 3.003 ± 0.706
3.66PheLeu: 3.66 ± 0.712
1.032PheMet: 1.032 ± 0.251
2.158PheAsn: 2.158 ± 0.576
2.909PhePro: 2.909 ± 0.623
1.314PheGln: 1.314 ± 0.409
3.19PheArg: 3.19 ± 0.446
2.534PheSer: 2.534 ± 0.495
1.501PheThr: 1.501 ± 0.311
3.097PheVal: 3.097 ± 0.488
0.469PheTrp: 0.469 ± 0.204
2.064PheTyr: 2.064 ± 0.53
0.0PheXaa: 0.0 ± 0.0
Gly
3.753GlyAla: 3.753 ± 0.655
0.188GlyCys: 0.188 ± 0.144
3.472GlyAsp: 3.472 ± 0.636
4.41GlyGlu: 4.41 ± 0.698
2.44GlyPhe: 2.44 ± 0.458
4.223GlyGly: 4.223 ± 0.561
1.501GlyHis: 1.501 ± 0.455
4.692GlyIle: 4.692 ± 0.97
4.692GlyLys: 4.692 ± 0.598
6.287GlyLeu: 6.287 ± 0.761
1.783GlyMet: 1.783 ± 0.479
2.721GlyAsn: 2.721 ± 0.427
1.032GlyPro: 1.032 ± 0.277
1.783GlyGln: 1.783 ± 0.457
3.847GlyArg: 3.847 ± 0.651
3.097GlySer: 3.097 ± 0.653
3.472GlyThr: 3.472 ± 0.545
3.472GlyVal: 3.472 ± 0.69
0.657GlyTrp: 0.657 ± 0.313
2.909GlyTyr: 2.909 ± 0.468
0.0GlyXaa: 0.0 ± 0.0
His
1.032HisAla: 1.032 ± 0.382
0.282HisCys: 0.282 ± 0.168
1.22HisAsp: 1.22 ± 0.378
1.126HisGlu: 1.126 ± 0.262
0.751HisPhe: 0.751 ± 0.251
0.845HisGly: 0.845 ± 0.292
0.375HisHis: 0.375 ± 0.209
1.314HisIle: 1.314 ± 0.398
1.22HisLys: 1.22 ± 0.32
1.595HisLeu: 1.595 ± 0.412
0.0HisMet: 0.0 ± 0.0
0.469HisAsn: 0.469 ± 0.237
0.845HisPro: 0.845 ± 0.358
0.563HisGln: 0.563 ± 0.201
0.845HisArg: 0.845 ± 0.251
0.845HisSer: 0.845 ± 0.277
0.845HisThr: 0.845 ± 0.35
1.22HisVal: 1.22 ± 0.365
0.0HisTrp: 0.0 ± 0.0
0.751HisTyr: 0.751 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
6.099IleAla: 6.099 ± 0.618
0.751IleCys: 0.751 ± 0.305
4.41IleAsp: 4.41 ± 0.59
6.85IleGlu: 6.85 ± 0.62
2.346IlePhe: 2.346 ± 0.498
4.879IleGly: 4.879 ± 0.833
1.314IleHis: 1.314 ± 0.376
4.692IleIle: 4.692 ± 0.702
5.818IleLys: 5.818 ± 0.832
3.847IleLeu: 3.847 ± 0.561
1.314IleMet: 1.314 ± 0.323
3.284IleAsn: 3.284 ± 0.551
3.097IlePro: 3.097 ± 0.413
2.534IleGln: 2.534 ± 0.451
4.504IleArg: 4.504 ± 0.576
4.692IleSer: 4.692 ± 0.804
3.753IleThr: 3.753 ± 0.581
4.598IleVal: 4.598 ± 0.633
0.563IleTrp: 0.563 ± 0.213
2.721IleTyr: 2.721 ± 0.535
0.0IleXaa: 0.0 ± 0.0
Lys
6.099LysAla: 6.099 ± 0.84
0.751LysCys: 0.751 ± 0.357
4.41LysAsp: 4.41 ± 0.691
6.193LysGlu: 6.193 ± 0.825
2.064LysPhe: 2.064 ± 0.34
4.41LysGly: 4.41 ± 0.576
1.314LysHis: 1.314 ± 0.421
5.536LysIle: 5.536 ± 0.648
6.944LysLys: 6.944 ± 1.11
5.536LysLeu: 5.536 ± 0.814
2.064LysMet: 2.064 ± 0.375
4.223LysAsn: 4.223 ± 0.588
2.44LysPro: 2.44 ± 0.471
3.941LysGln: 3.941 ± 0.642
5.067LysArg: 5.067 ± 0.706
4.504LysSer: 4.504 ± 0.809
4.973LysThr: 4.973 ± 0.888
5.442LysVal: 5.442 ± 0.723
1.126LysTrp: 1.126 ± 0.333
3.097LysTyr: 3.097 ± 0.522
0.0LysXaa: 0.0 ± 0.0
Leu
5.63LeuAla: 5.63 ± 0.821
0.751LeuCys: 0.751 ± 0.305
5.818LeuAsp: 5.818 ± 0.784
6.193LeuGlu: 6.193 ± 0.635
3.284LeuPhe: 3.284 ± 0.565
5.818LeuGly: 5.818 ± 0.82
1.501LeuHis: 1.501 ± 0.389
5.442LeuIle: 5.442 ± 0.754
7.131LeuLys: 7.131 ± 0.933
8.07LeuLeu: 8.07 ± 0.78
2.346LeuMet: 2.346 ± 0.456
4.223LeuAsn: 4.223 ± 0.608
2.815LeuPro: 2.815 ± 0.434
3.566LeuGln: 3.566 ± 0.577
4.879LeuArg: 4.879 ± 0.712
4.973LeuSer: 4.973 ± 0.956
4.504LeuThr: 4.504 ± 0.777
5.161LeuVal: 5.161 ± 0.754
0.469LeuTrp: 0.469 ± 0.181
3.378LeuTyr: 3.378 ± 0.484
0.0LeuXaa: 0.0 ± 0.0
Met
2.158MetAla: 2.158 ± 0.461
0.375MetCys: 0.375 ± 0.23
1.22MetAsp: 1.22 ± 0.362
2.158MetGlu: 2.158 ± 0.499
1.314MetPhe: 1.314 ± 0.311
1.314MetGly: 1.314 ± 0.254
0.375MetHis: 0.375 ± 0.215
1.971MetIle: 1.971 ± 0.452
2.534MetLys: 2.534 ± 0.43
2.158MetLeu: 2.158 ± 0.354
0.563MetMet: 0.563 ± 0.215
1.22MetAsn: 1.22 ± 0.286
0.751MetPro: 0.751 ± 0.223
0.938MetGln: 0.938 ± 0.33
1.501MetArg: 1.501 ± 0.396
1.314MetSer: 1.314 ± 0.301
2.158MetThr: 2.158 ± 0.482
1.22MetVal: 1.22 ± 0.365
0.375MetTrp: 0.375 ± 0.189
0.938MetTyr: 0.938 ± 0.292
0.0MetXaa: 0.0 ± 0.0
Asn
3.472AsnAla: 3.472 ± 1.291
0.375AsnCys: 0.375 ± 0.187
2.158AsnAsp: 2.158 ± 0.499
3.378AsnGlu: 3.378 ± 0.594
1.689AsnPhe: 1.689 ± 0.336
3.847AsnGly: 3.847 ± 0.496
0.563AsnHis: 0.563 ± 0.268
3.378AsnIle: 3.378 ± 0.56
2.44AsnLys: 2.44 ± 0.488
3.378AsnLeu: 3.378 ± 0.568
1.314AsnMet: 1.314 ± 0.247
2.252AsnAsn: 2.252 ± 0.507
2.627AsnPro: 2.627 ± 0.667
1.501AsnGln: 1.501 ± 0.391
2.346AsnArg: 2.346 ± 0.418
2.627AsnSer: 2.627 ± 0.46
2.721AsnThr: 2.721 ± 0.474
3.19AsnVal: 3.19 ± 0.555
0.282AsnTrp: 0.282 ± 0.139
1.595AsnTyr: 1.595 ± 0.548
0.0AsnXaa: 0.0 ± 0.0
Pro
1.595ProAla: 1.595 ± 0.325
0.094ProCys: 0.094 ± 0.088
2.534ProAsp: 2.534 ± 0.38
3.097ProGlu: 3.097 ± 0.493
1.971ProPhe: 1.971 ± 0.452
2.627ProGly: 2.627 ± 0.452
0.751ProHis: 0.751 ± 0.302
2.534ProIle: 2.534 ± 0.536
3.003ProLys: 3.003 ± 0.614
2.346ProLeu: 2.346 ± 0.391
0.469ProMet: 0.469 ± 0.215
1.032ProAsn: 1.032 ± 0.275
1.126ProPro: 1.126 ± 0.29
1.22ProGln: 1.22 ± 0.347
1.501ProArg: 1.501 ± 0.367
2.534ProSer: 2.534 ± 0.481
1.689ProThr: 1.689 ± 0.331
2.158ProVal: 2.158 ± 0.416
0.751ProTrp: 0.751 ± 0.235
1.971ProTyr: 1.971 ± 0.598
0.0ProXaa: 0.0 ± 0.0
Gln
3.378GlnAla: 3.378 ± 0.871
0.375GlnCys: 0.375 ± 0.163
1.22GlnAsp: 1.22 ± 0.276
2.627GlnGlu: 2.627 ± 0.459
1.22GlnPhe: 1.22 ± 0.313
1.877GlnGly: 1.877 ± 0.508
0.563GlnHis: 0.563 ± 0.215
2.346GlnIle: 2.346 ± 0.414
4.035GlnLys: 4.035 ± 0.454
2.909GlnLeu: 2.909 ± 0.683
1.126GlnMet: 1.126 ± 0.353
1.877GlnAsn: 1.877 ± 0.446
1.501GlnPro: 1.501 ± 0.309
1.501GlnGln: 1.501 ± 0.378
2.627GlnArg: 2.627 ± 0.428
1.689GlnSer: 1.689 ± 0.34
2.158GlnThr: 2.158 ± 0.458
3.378GlnVal: 3.378 ± 0.67
0.751GlnTrp: 0.751 ± 0.276
1.501GlnTyr: 1.501 ± 0.332
0.0GlnXaa: 0.0 ± 0.0
Arg
3.097ArgAla: 3.097 ± 0.501
0.751ArgCys: 0.751 ± 0.314
3.66ArgAsp: 3.66 ± 0.631
4.316ArgGlu: 4.316 ± 0.679
3.003ArgPhe: 3.003 ± 0.568
3.003ArgGly: 3.003 ± 0.427
0.845ArgHis: 0.845 ± 0.272
3.941ArgIle: 3.941 ± 0.692
6.193ArgLys: 6.193 ± 0.898
4.879ArgLeu: 4.879 ± 0.637
2.346ArgMet: 2.346 ± 0.462
2.534ArgAsn: 2.534 ± 0.454
2.158ArgPro: 2.158 ± 0.516
2.346ArgGln: 2.346 ± 0.431
3.472ArgArg: 3.472 ± 0.6
2.346ArgSer: 2.346 ± 0.46
2.627ArgThr: 2.627 ± 0.416
3.941ArgVal: 3.941 ± 0.738
0.563ArgTrp: 0.563 ± 0.257
2.158ArgTyr: 2.158 ± 0.53
0.0ArgXaa: 0.0 ± 0.0
Ser
2.534SerAla: 2.534 ± 0.67
0.282SerCys: 0.282 ± 0.152
3.378SerAsp: 3.378 ± 0.631
3.941SerGlu: 3.941 ± 0.708
3.284SerPhe: 3.284 ± 0.463
2.815SerGly: 2.815 ± 0.497
0.845SerHis: 0.845 ± 0.258
3.941SerIle: 3.941 ± 0.634
3.66SerLys: 3.66 ± 0.603
5.724SerLeu: 5.724 ± 0.596
1.314SerMet: 1.314 ± 0.366
2.064SerAsn: 2.064 ± 0.508
1.408SerPro: 1.408 ± 0.314
1.783SerGln: 1.783 ± 0.476
2.815SerArg: 2.815 ± 0.494
3.378SerSer: 3.378 ± 0.65
3.097SerThr: 3.097 ± 0.576
3.941SerVal: 3.941 ± 0.638
0.845SerTrp: 0.845 ± 0.22
1.314SerTyr: 1.314 ± 0.533
0.0SerXaa: 0.0 ± 0.0
Thr
4.223ThrAla: 4.223 ± 0.62
0.188ThrCys: 0.188 ± 0.115
1.877ThrAsp: 1.877 ± 0.355
4.129ThrGlu: 4.129 ± 0.589
1.783ThrPhe: 1.783 ± 0.421
3.19ThrGly: 3.19 ± 0.584
0.938ThrHis: 0.938 ± 0.232
4.504ThrIle: 4.504 ± 0.538
3.19ThrLys: 3.19 ± 0.502
4.879ThrLeu: 4.879 ± 0.854
1.032ThrMet: 1.032 ± 0.253
3.472ThrAsn: 3.472 ± 0.65
2.534ThrPro: 2.534 ± 0.477
1.501ThrGln: 1.501 ± 0.371
2.346ThrArg: 2.346 ± 0.443
3.378ThrSer: 3.378 ± 0.671
1.971ThrThr: 1.971 ± 0.56
3.847ThrVal: 3.847 ± 0.589
0.375ThrTrp: 0.375 ± 0.162
2.627ThrTyr: 2.627 ± 0.637
0.0ThrXaa: 0.0 ± 0.0
Val
4.035ValAla: 4.035 ± 0.581
0.657ValCys: 0.657 ± 0.271
4.223ValAsp: 4.223 ± 0.709
5.818ValGlu: 5.818 ± 0.842
3.19ValPhe: 3.19 ± 0.491
4.223ValGly: 4.223 ± 0.589
1.595ValHis: 1.595 ± 0.454
4.786ValIle: 4.786 ± 0.638
6.193ValLys: 6.193 ± 0.782
6.005ValLeu: 6.005 ± 0.748
2.158ValMet: 2.158 ± 0.305
2.815ValAsn: 2.815 ± 0.487
2.815ValPro: 2.815 ± 0.509
2.252ValGln: 2.252 ± 0.515
4.223ValArg: 4.223 ± 0.67
2.815ValSer: 2.815 ± 0.645
3.66ValThr: 3.66 ± 0.534
4.786ValVal: 4.786 ± 0.759
0.938ValTrp: 0.938 ± 0.395
2.44ValTyr: 2.44 ± 0.475
0.0ValXaa: 0.0 ± 0.0
Trp
0.845TrpAla: 0.845 ± 0.272
0.0TrpCys: 0.0 ± 0.0
0.563TrpAsp: 0.563 ± 0.197
0.751TrpGlu: 0.751 ± 0.351
0.469TrpPhe: 0.469 ± 0.313
0.282TrpGly: 0.282 ± 0.128
0.094TrpHis: 0.094 ± 0.083
0.563TrpIle: 0.563 ± 0.211
0.657TrpLys: 0.657 ± 0.23
1.126TrpLeu: 1.126 ± 0.351
0.469TrpMet: 0.469 ± 0.192
0.938TrpAsn: 0.938 ± 0.672
0.094TrpPro: 0.094 ± 0.098
0.751TrpGln: 0.751 ± 0.25
0.845TrpArg: 0.845 ± 0.239
0.657TrpSer: 0.657 ± 0.238
0.375TrpThr: 0.375 ± 0.192
0.845TrpVal: 0.845 ± 0.236
0.094TrpTrp: 0.094 ± 0.098
0.563TrpTyr: 0.563 ± 0.244
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.534TyrAla: 2.534 ± 0.5
0.375TyrCys: 0.375 ± 0.217
2.064TyrAsp: 2.064 ± 0.435
3.284TyrGlu: 3.284 ± 0.74
2.158TyrPhe: 2.158 ± 0.508
2.534TyrGly: 2.534 ± 0.481
0.563TyrHis: 0.563 ± 0.227
2.158TyrIle: 2.158 ± 0.418
3.66TyrLys: 3.66 ± 0.775
2.815TyrLeu: 2.815 ± 0.475
1.126TyrMet: 1.126 ± 0.336
1.408TyrAsn: 1.408 ± 0.386
1.971TyrPro: 1.971 ± 0.432
1.314TyrGln: 1.314 ± 0.335
3.003TyrArg: 3.003 ± 0.571
2.534TyrSer: 2.534 ± 0.494
2.064TyrThr: 2.064 ± 0.371
3.472TyrVal: 3.472 ± 0.526
0.375TyrTrp: 0.375 ± 0.164
1.783TyrTyr: 1.783 ± 0.472
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (10658 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski