Amino acid dipepetide frequency for Cyanophage PP

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.701AlaAla: 5.701 ± 0.63
0.304AlaCys: 0.304 ± 0.188
4.789AlaAsp: 4.789 ± 0.686
4.105AlaGlu: 4.105 ± 0.544
2.965AlaPhe: 2.965 ± 0.47
4.029AlaGly: 4.029 ± 0.804
1.292AlaHis: 1.292 ± 0.294
2.813AlaIle: 2.813 ± 0.46
4.561AlaLys: 4.561 ± 0.781
8.058AlaLeu: 8.058 ± 0.909
1.596AlaMet: 1.596 ± 0.358
3.801AlaAsn: 3.801 ± 0.626
2.357AlaPro: 2.357 ± 0.484
4.561AlaGln: 4.561 ± 0.993
3.877AlaArg: 3.877 ± 0.447
5.473AlaSer: 5.473 ± 0.467
5.777AlaThr: 5.777 ± 0.709
6.385AlaVal: 6.385 ± 0.961
0.532AlaTrp: 0.532 ± 0.191
3.117AlaTyr: 3.117 ± 0.571
0.0AlaXaa: 0.0 ± 0.0
Cys
0.38CysAla: 0.38 ± 0.182
0.0CysCys: 0.0 ± 0.0
0.152CysAsp: 0.152 ± 0.136
0.608CysGlu: 0.608 ± 0.259
0.304CysPhe: 0.304 ± 0.166
0.608CysGly: 0.608 ± 0.256
0.304CysHis: 0.304 ± 0.156
0.076CysIle: 0.076 ± 0.071
0.228CysLys: 0.228 ± 0.128
0.912CysLeu: 0.912 ± 0.312
0.304CysMet: 0.304 ± 0.145
0.456CysAsn: 0.456 ± 0.23
0.38CysPro: 0.38 ± 0.141
0.304CysGln: 0.304 ± 0.171
0.456CysArg: 0.456 ± 0.241
0.38CysSer: 0.38 ± 0.172
0.532CysThr: 0.532 ± 0.179
0.456CysVal: 0.456 ± 0.195
0.0CysTrp: 0.0 ± 0.0
0.152CysTyr: 0.152 ± 0.117
0.0CysXaa: 0.0 ± 0.0
Asp
4.561AspAla: 4.561 ± 0.678
0.38AspCys: 0.38 ± 0.159
4.561AspAsp: 4.561 ± 0.695
5.397AspGlu: 5.397 ± 0.77
1.976AspPhe: 1.976 ± 0.35
4.789AspGly: 4.789 ± 0.658
1.064AspHis: 1.064 ± 0.276
3.497AspIle: 3.497 ± 0.701
4.333AspLys: 4.333 ± 0.702
6.385AspLeu: 6.385 ± 0.57
1.216AspMet: 1.216 ± 0.221
3.269AspAsn: 3.269 ± 0.44
2.737AspPro: 2.737 ± 0.512
1.976AspGln: 1.976 ± 0.448
3.117AspArg: 3.117 ± 0.558
2.585AspSer: 2.585 ± 0.479
5.549AspThr: 5.549 ± 0.654
6.157AspVal: 6.157 ± 0.739
0.76AspTrp: 0.76 ± 0.215
2.357AspTyr: 2.357 ± 0.405
0.0AspXaa: 0.0 ± 0.0
Glu
6.309GluAla: 6.309 ± 0.711
0.304GluCys: 0.304 ± 0.18
4.409GluAsp: 4.409 ± 0.486
2.813GluGlu: 2.813 ± 0.504
1.52GluPhe: 1.52 ± 0.346
2.965GluGly: 2.965 ± 0.519
0.912GluHis: 0.912 ± 0.25
1.216GluIle: 1.216 ± 0.248
1.444GluLys: 1.444 ± 0.277
7.374GluLeu: 7.374 ± 0.946
1.064GluMet: 1.064 ± 0.319
2.052GluAsn: 2.052 ± 0.458
2.661GluPro: 2.661 ± 0.645
3.801GluGln: 3.801 ± 0.684
4.789GluArg: 4.789 ± 0.609
2.128GluSer: 2.128 ± 0.439
3.877GluThr: 3.877 ± 0.605
6.613GluVal: 6.613 ± 0.681
0.456GluTrp: 0.456 ± 0.219
2.509GluTyr: 2.509 ± 0.432
0.0GluXaa: 0.0 ± 0.0
Phe
2.889PheAla: 2.889 ± 0.519
0.152PheCys: 0.152 ± 0.107
3.041PheAsp: 3.041 ± 0.547
1.976PheGlu: 1.976 ± 0.438
0.912PhePhe: 0.912 ± 0.31
2.965PheGly: 2.965 ± 0.502
0.532PheHis: 0.532 ± 0.27
1.064PheIle: 1.064 ± 0.378
1.976PheLys: 1.976 ± 0.407
1.748PheLeu: 1.748 ± 0.361
0.38PheMet: 0.38 ± 0.142
2.433PheAsn: 2.433 ± 0.466
0.608PhePro: 0.608 ± 0.172
1.14PheGln: 1.14 ± 0.235
1.824PheArg: 1.824 ± 0.353
3.041PheSer: 3.041 ± 0.557
3.421PheThr: 3.421 ± 0.74
2.509PheVal: 2.509 ± 0.557
0.228PheTrp: 0.228 ± 0.139
1.064PheTyr: 1.064 ± 0.241
0.0PheXaa: 0.0 ± 0.0
Gly
6.081GlyAla: 6.081 ± 0.95
0.76GlyCys: 0.76 ± 0.371
4.485GlyAsp: 4.485 ± 0.495
3.041GlyGlu: 3.041 ± 0.671
2.433GlyPhe: 2.433 ± 0.376
5.093GlyGly: 5.093 ± 0.664
1.064GlyHis: 1.064 ± 0.29
2.281GlyIle: 2.281 ± 0.422
3.573GlyLys: 3.573 ± 0.445
5.777GlyLeu: 5.777 ± 0.852
1.064GlyMet: 1.064 ± 0.227
3.041GlyAsn: 3.041 ± 0.569
0.0GlyPro: 0.0 ± 0.0
2.585GlyGln: 2.585 ± 0.365
3.953GlyArg: 3.953 ± 0.803
4.561GlySer: 4.561 ± 0.643
5.473GlyThr: 5.473 ± 0.871
4.865GlyVal: 4.865 ± 0.573
0.456GlyTrp: 0.456 ± 0.221
4.105GlyTyr: 4.105 ± 0.701
0.0GlyXaa: 0.0 ± 0.0
His
1.14HisAla: 1.14 ± 0.209
0.152HisCys: 0.152 ± 0.113
0.988HisAsp: 0.988 ± 0.302
1.444HisGlu: 1.444 ± 0.37
0.456HisPhe: 0.456 ± 0.17
0.76HisGly: 0.76 ± 0.209
0.228HisHis: 0.228 ± 0.128
0.912HisIle: 0.912 ± 0.27
0.912HisLys: 0.912 ± 0.314
1.9HisLeu: 1.9 ± 0.332
0.38HisMet: 0.38 ± 0.186
0.684HisAsn: 0.684 ± 0.238
1.14HisPro: 1.14 ± 0.352
0.456HisGln: 0.456 ± 0.182
0.684HisArg: 0.684 ± 0.23
0.836HisSer: 0.836 ± 0.269
0.988HisThr: 0.988 ± 0.381
0.988HisVal: 0.988 ± 0.246
0.532HisTrp: 0.532 ± 0.132
0.608HisTyr: 0.608 ± 0.315
0.0HisXaa: 0.0 ± 0.0
Ile
2.128IleAla: 2.128 ± 0.459
0.38IleCys: 0.38 ± 0.16
2.585IleAsp: 2.585 ± 0.583
1.596IleGlu: 1.596 ± 0.334
1.14IlePhe: 1.14 ± 0.363
2.965IleGly: 2.965 ± 0.457
0.76IleHis: 0.76 ± 0.286
1.064IleIle: 1.064 ± 0.404
2.585IleLys: 2.585 ± 0.37
2.281IleLeu: 2.281 ± 0.286
0.912IleMet: 0.912 ± 0.217
1.672IleAsn: 1.672 ± 0.363
2.433IlePro: 2.433 ± 0.432
1.368IleGln: 1.368 ± 0.361
2.509IleArg: 2.509 ± 0.533
2.204IleSer: 2.204 ± 0.525
2.204IleThr: 2.204 ± 0.374
1.672IleVal: 1.672 ± 0.434
0.38IleTrp: 0.38 ± 0.233
1.596IleTyr: 1.596 ± 0.36
0.0IleXaa: 0.0 ± 0.0
Lys
5.093LysAla: 5.093 ± 0.653
0.304LysCys: 0.304 ± 0.115
3.497LysAsp: 3.497 ± 0.681
3.953LysGlu: 3.953 ± 0.717
1.444LysPhe: 1.444 ± 0.286
2.433LysGly: 2.433 ± 0.443
1.14LysHis: 1.14 ± 0.27
0.836LysIle: 0.836 ± 0.201
2.965LysLys: 2.965 ± 0.51
5.169LysLeu: 5.169 ± 0.732
0.76LysMet: 0.76 ± 0.301
2.357LysAsn: 2.357 ± 0.613
3.269LysPro: 3.269 ± 0.857
4.029LysGln: 4.029 ± 0.583
4.105LysArg: 4.105 ± 0.453
2.965LysSer: 2.965 ± 0.443
3.649LysThr: 3.649 ± 0.643
4.029LysVal: 4.029 ± 0.503
0.532LysTrp: 0.532 ± 0.231
2.433LysTyr: 2.433 ± 0.541
0.0LysXaa: 0.0 ± 0.0
Leu
6.157LeuAla: 6.157 ± 0.702
1.14LeuCys: 1.14 ± 0.305
6.233LeuAsp: 6.233 ± 0.757
4.789LeuGlu: 4.789 ± 0.641
2.281LeuPhe: 2.281 ± 0.426
5.549LeuGly: 5.549 ± 0.582
1.444LeuHis: 1.444 ± 0.467
2.813LeuIle: 2.813 ± 0.473
4.105LeuLys: 4.105 ± 0.721
8.286LeuLeu: 8.286 ± 0.899
1.824LeuMet: 1.824 ± 0.417
3.269LeuAsn: 3.269 ± 0.545
4.941LeuPro: 4.941 ± 1.125
4.713LeuGln: 4.713 ± 0.807
6.233LeuArg: 6.233 ± 0.926
8.438LeuSer: 8.438 ± 0.62
6.994LeuThr: 6.994 ± 0.839
6.994LeuVal: 6.994 ± 0.79
0.76LeuTrp: 0.76 ± 0.225
3.117LeuTyr: 3.117 ± 0.739
0.0LeuXaa: 0.0 ± 0.0
Met
1.216MetAla: 1.216 ± 0.292
0.152MetCys: 0.152 ± 0.112
0.608MetAsp: 0.608 ± 0.283
0.684MetGlu: 0.684 ± 0.192
0.684MetPhe: 0.684 ± 0.203
0.836MetGly: 0.836 ± 0.317
0.456MetHis: 0.456 ± 0.186
0.456MetIle: 0.456 ± 0.165
1.368MetLys: 1.368 ± 0.223
1.9MetLeu: 1.9 ± 0.384
0.456MetMet: 0.456 ± 0.146
0.836MetAsn: 0.836 ± 0.208
0.912MetPro: 0.912 ± 0.399
1.216MetGln: 1.216 ± 0.274
0.988MetArg: 0.988 ± 0.275
2.128MetSer: 2.128 ± 0.419
1.596MetThr: 1.596 ± 0.361
0.836MetVal: 0.836 ± 0.259
0.152MetTrp: 0.152 ± 0.144
0.76MetTyr: 0.76 ± 0.205
0.0MetXaa: 0.0 ± 0.0
Asn
3.117AsnAla: 3.117 ± 0.461
0.38AsnCys: 0.38 ± 0.165
2.128AsnAsp: 2.128 ± 0.362
2.509AsnGlu: 2.509 ± 0.651
1.672AsnPhe: 1.672 ± 0.343
2.433AsnGly: 2.433 ± 0.44
0.608AsnHis: 0.608 ± 0.187
2.509AsnIle: 2.509 ± 0.31
3.041AsnLys: 3.041 ± 0.441
3.953AsnLeu: 3.953 ± 0.604
0.76AsnMet: 0.76 ± 0.258
2.661AsnAsn: 2.661 ± 0.527
2.965AsnPro: 2.965 ± 0.517
1.976AsnGln: 1.976 ± 0.404
2.052AsnArg: 2.052 ± 0.364
1.824AsnSer: 1.824 ± 0.318
3.877AsnThr: 3.877 ± 0.637
3.573AsnVal: 3.573 ± 0.549
0.684AsnTrp: 0.684 ± 0.204
2.281AsnTyr: 2.281 ± 0.524
0.0AsnXaa: 0.0 ± 0.0
Pro
3.193ProAla: 3.193 ± 0.391
0.152ProCys: 0.152 ± 0.104
3.345ProAsp: 3.345 ± 0.61
3.193ProGlu: 3.193 ± 0.939
1.368ProPhe: 1.368 ± 0.381
2.813ProGly: 2.813 ± 0.663
0.532ProHis: 0.532 ± 0.223
1.14ProIle: 1.14 ± 0.329
2.357ProLys: 2.357 ± 0.491
3.801ProLeu: 3.801 ± 0.676
0.836ProMet: 0.836 ± 0.256
1.748ProAsn: 1.748 ± 0.472
1.52ProPro: 1.52 ± 0.506
1.9ProGln: 1.9 ± 0.432
1.9ProArg: 1.9 ± 0.445
4.105ProSer: 4.105 ± 0.823
3.877ProThr: 3.877 ± 0.832
3.877ProVal: 3.877 ± 0.721
0.456ProTrp: 0.456 ± 0.15
1.9ProTyr: 1.9 ± 0.367
0.0ProXaa: 0.0 ± 0.0
Gln
6.918GlnAla: 6.918 ± 0.894
0.076GlnCys: 0.076 ± 0.092
2.509GlnAsp: 2.509 ± 0.504
2.813GlnGlu: 2.813 ± 0.519
1.9GlnPhe: 1.9 ± 0.36
3.041GlnGly: 3.041 ± 0.734
0.684GlnHis: 0.684 ± 0.298
1.216GlnIle: 1.216 ± 0.225
2.661GlnLys: 2.661 ± 0.495
4.409GlnLeu: 4.409 ± 0.692
1.444GlnMet: 1.444 ± 0.414
1.748GlnAsn: 1.748 ± 0.362
2.281GlnPro: 2.281 ± 0.463
6.081GlnGln: 6.081 ± 1.6
3.117GlnArg: 3.117 ± 0.451
3.041GlnSer: 3.041 ± 0.537
3.801GlnThr: 3.801 ± 0.482
3.041GlnVal: 3.041 ± 0.441
0.684GlnTrp: 0.684 ± 0.259
2.357GlnTyr: 2.357 ± 0.504
0.0GlnXaa: 0.0 ± 0.0
Arg
4.029ArgAla: 4.029 ± 0.443
0.456ArgCys: 0.456 ± 0.158
3.345ArgAsp: 3.345 ± 0.456
4.029ArgGlu: 4.029 ± 0.735
2.052ArgPhe: 2.052 ± 0.464
3.117ArgGly: 3.117 ± 0.622
0.76ArgHis: 0.76 ± 0.213
2.433ArgIle: 2.433 ± 0.384
3.421ArgLys: 3.421 ± 0.486
5.473ArgLeu: 5.473 ± 0.678
1.216ArgMet: 1.216 ± 0.287
2.813ArgAsn: 2.813 ± 0.454
2.585ArgPro: 2.585 ± 0.513
3.801ArgGln: 3.801 ± 0.662
4.029ArgArg: 4.029 ± 0.77
2.965ArgSer: 2.965 ± 0.477
3.801ArgThr: 3.801 ± 0.441
4.181ArgVal: 4.181 ± 0.429
0.38ArgTrp: 0.38 ± 0.139
2.813ArgTyr: 2.813 ± 0.65
0.0ArgXaa: 0.0 ± 0.0
Ser
4.333SerAla: 4.333 ± 0.733
0.456SerCys: 0.456 ± 0.208
5.017SerAsp: 5.017 ± 0.522
4.561SerGlu: 4.561 ± 0.692
2.661SerPhe: 2.661 ± 0.52
5.549SerGly: 5.549 ± 0.637
0.988SerHis: 0.988 ± 0.296
1.9SerIle: 1.9 ± 0.297
3.725SerLys: 3.725 ± 0.493
5.169SerLeu: 5.169 ± 0.658
1.14SerMet: 1.14 ± 0.327
2.889SerAsn: 2.889 ± 0.533
3.497SerPro: 3.497 ± 0.831
3.421SerGln: 3.421 ± 0.574
3.193SerArg: 3.193 ± 0.354
5.245SerSer: 5.245 ± 0.943
4.561SerThr: 4.561 ± 0.489
4.561SerVal: 4.561 ± 0.485
1.14SerTrp: 1.14 ± 0.376
2.965SerTyr: 2.965 ± 0.407
0.0SerXaa: 0.0 ± 0.0
Thr
4.941ThrAla: 4.941 ± 0.494
0.38ThrCys: 0.38 ± 0.216
4.409ThrAsp: 4.409 ± 0.582
3.953ThrGlu: 3.953 ± 1.045
3.421ThrPhe: 3.421 ± 0.648
5.777ThrGly: 5.777 ± 0.822
0.912ThrHis: 0.912 ± 0.282
2.737ThrIle: 2.737 ± 0.451
4.485ThrLys: 4.485 ± 0.636
6.309ThrLeu: 6.309 ± 0.786
0.988ThrMet: 0.988 ± 0.316
2.737ThrAsn: 2.737 ± 0.568
4.713ThrPro: 4.713 ± 0.738
3.877ThrGln: 3.877 ± 0.466
3.877ThrArg: 3.877 ± 0.561
4.941ThrSer: 4.941 ± 0.524
7.754ThrThr: 7.754 ± 1.208
6.081ThrVal: 6.081 ± 0.892
1.368ThrTrp: 1.368 ± 0.265
4.029ThrTyr: 4.029 ± 0.509
0.0ThrXaa: 0.0 ± 0.0
Val
4.257ValAla: 4.257 ± 0.538
0.76ValCys: 0.76 ± 0.237
5.929ValAsp: 5.929 ± 0.529
4.865ValGlu: 4.865 ± 0.532
2.585ValPhe: 2.585 ± 0.426
4.485ValGly: 4.485 ± 0.453
1.52ValHis: 1.52 ± 0.488
3.193ValIle: 3.193 ± 0.464
4.409ValLys: 4.409 ± 0.604
6.157ValLeu: 6.157 ± 0.735
0.836ValMet: 0.836 ± 0.198
3.269ValAsn: 3.269 ± 0.612
4.029ValPro: 4.029 ± 0.722
3.953ValGln: 3.953 ± 0.609
4.409ValArg: 4.409 ± 0.697
7.07ValSer: 7.07 ± 0.659
5.777ValThr: 5.777 ± 0.661
6.537ValVal: 6.537 ± 0.712
0.684ValTrp: 0.684 ± 0.263
3.345ValTyr: 3.345 ± 0.515
0.0ValXaa: 0.0 ± 0.0
Trp
0.988TrpAla: 0.988 ± 0.222
0.152TrpCys: 0.152 ± 0.104
0.608TrpAsp: 0.608 ± 0.225
0.684TrpGlu: 0.684 ± 0.213
0.456TrpPhe: 0.456 ± 0.173
0.684TrpGly: 0.684 ± 0.201
0.304TrpHis: 0.304 ± 0.16
0.152TrpIle: 0.152 ± 0.1
0.38TrpLys: 0.38 ± 0.163
1.368TrpLeu: 1.368 ± 0.351
0.152TrpMet: 0.152 ± 0.099
0.836TrpAsn: 0.836 ± 0.267
0.0TrpPro: 0.0 ± 0.0
0.532TrpGln: 0.532 ± 0.166
0.304TrpArg: 0.304 ± 0.155
0.988TrpSer: 0.988 ± 0.348
0.608TrpThr: 0.608 ± 0.266
0.988TrpVal: 0.988 ± 0.3
0.304TrpTrp: 0.304 ± 0.112
0.608TrpTyr: 0.608 ± 0.223
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.661TyrAla: 2.661 ± 0.409
0.152TyrCys: 0.152 ± 0.122
4.029TyrAsp: 4.029 ± 0.691
2.204TyrGlu: 2.204 ± 0.395
1.672TyrPhe: 1.672 ± 0.415
3.801TyrGly: 3.801 ± 0.601
0.684TyrHis: 0.684 ± 0.269
2.128TyrIle: 2.128 ± 0.457
2.433TyrLys: 2.433 ± 0.402
3.649TyrLeu: 3.649 ± 0.713
0.836TyrMet: 0.836 ± 0.292
2.433TyrAsn: 2.433 ± 0.445
0.988TyrPro: 0.988 ± 0.285
2.128TyrGln: 2.128 ± 0.477
2.357TyrArg: 2.357 ± 0.531
2.052TyrSer: 2.052 ± 0.458
3.573TyrThr: 3.573 ± 0.682
3.725TyrVal: 3.725 ± 0.579
0.608TyrTrp: 0.608 ± 0.231
2.052TyrTyr: 2.052 ± 0.316
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (13156 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski