Pedobacter changchengzhani

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3293 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5MHP5|A0A4R5MHP5_9SPHI Orotate phosphoribosyltransferase OS=Pedobacter changchengzhani OX=2529274 GN=pyrE PE=3 SV=1
MM1 pKa = 7.44NVTTNNEE8 pKa = 4.08LKK10 pKa = 9.77STIGLSGLYY19 pKa = 10.17YY20 pKa = 11.33VMDD23 pKa = 4.52PEE25 pKa = 5.78IGLNIVDD32 pKa = 4.95LGLIYY37 pKa = 9.94EE38 pKa = 4.29IQFDD42 pKa = 4.1EE43 pKa = 4.84DD44 pKa = 3.07KK45 pKa = 11.36HH46 pKa = 7.59LITVIMTLTTQFCPMGEE63 pKa = 4.44SIVDD67 pKa = 3.4ATTQAMQEE75 pKa = 3.96RR76 pKa = 11.84FQDD79 pKa = 3.57YY80 pKa = 10.22EE81 pKa = 4.57INVKK85 pKa = 8.77LTFNPPWNPDD95 pKa = 3.52MISEE99 pKa = 4.28EE100 pKa = 4.24GNEE103 pKa = 4.01FLNAA107 pKa = 3.91

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5MLE4|A0A4R5MLE4_9SPHI EamA family transporter OS=Pedobacter changchengzhani OX=2529274 GN=EZJ43_08260 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.57NRR4 pKa = 11.84IRR6 pKa = 11.84RR7 pKa = 11.84NQLRR11 pKa = 11.84YY12 pKa = 9.35LQILIRR18 pKa = 11.84LAFLIVPIVILYY30 pKa = 8.35FLVVFNYY37 pKa = 9.95NPHH40 pKa = 5.77EE41 pKa = 4.17RR42 pKa = 11.84CIGDD46 pKa = 3.52EE47 pKa = 4.22HH48 pKa = 6.91RR49 pKa = 11.84HH50 pKa = 4.52TMGPMFGFLIFSGFIVVIWLLAMIIEE76 pKa = 5.04LIYY79 pKa = 10.84RR80 pKa = 11.84RR81 pKa = 11.84FDD83 pKa = 3.37KK84 pKa = 10.87NKK86 pKa = 9.85KK87 pKa = 7.16VAYY90 pKa = 8.28WLIFLVVMASLAIMFFII107 pKa = 5.54

Molecular weight: 12.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3293

0

3293

1105995

25

2675

335.9

37.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.98 ± 0.047

0.789 ± 0.013

5.471 ± 0.032

5.85 ± 0.047

5.314 ± 0.038

6.48 ± 0.045

1.674 ± 0.02

7.997 ± 0.042

8.099 ± 0.05

9.526 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.019

6.241 ± 0.042

3.462 ± 0.022

3.426 ± 0.023

3.333 ± 0.027

6.234 ± 0.035

5.643 ± 0.043

6.202 ± 0.03

1.045 ± 0.017

4.044 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski