Rhodotorula sp. CCFEE 5036

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Microbotryomycetes; Sporidiobolales; Sporidiobolaceae; Rhodotorula; unclassified Rhodotorula

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6416 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U0VYX4|A0A4U0VYX4_9BASI Uncharacterized protein OS=Rhodotorula sp. CCFEE 5036 OX=1965284 GN=B0A53_02467 PE=4 SV=1
MM1 pKa = 7.13LQSAPSGADD10 pKa = 2.95NDD12 pKa = 4.02YY13 pKa = 11.62DD14 pKa = 4.45DD15 pKa = 6.26LYY17 pKa = 11.54GDD19 pKa = 5.03LYY21 pKa = 10.5PAEE24 pKa = 4.34TEE26 pKa = 4.07AAPAASTSAAPSASATTAPAAASPAGSTSTSAATGAPAAIGYY68 pKa = 9.72GGDD71 pKa = 3.52AARR74 pKa = 11.84PAQLPQVPTGAAMPAAGGMVHH95 pKa = 7.6DD96 pKa = 5.07IGIPRR101 pKa = 11.84PQALQANALASKK113 pKa = 8.94TADD116 pKa = 3.35QLDD119 pKa = 3.82EE120 pKa = 4.28GG121 pKa = 4.45

Molecular weight: 11.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V5NEC6|A0A4V5NEC6_9BASI Uncharacterized protein OS=Rhodotorula sp. CCFEE 5036 OX=1965284 GN=B0A53_00046 PE=4 SV=1
MM1 pKa = 7.7SRR3 pKa = 11.84LLSNLPRR10 pKa = 11.84LVFANAARR18 pKa = 11.84PSSWAAVSPRR28 pKa = 11.84PSSAQQQHH36 pKa = 5.95LRR38 pKa = 11.84SISTALLSRR47 pKa = 11.84VQAPLAQPALVAPPVSLVQPAAGQTRR73 pKa = 11.84AFKK76 pKa = 10.21MPRR79 pKa = 11.84SMRR82 pKa = 11.84KK83 pKa = 8.77VSSPVARR90 pKa = 11.84NTGKK94 pKa = 10.8GKK96 pKa = 7.44TARR99 pKa = 11.84KK100 pKa = 9.4HH101 pKa = 4.6SARR104 pKa = 11.84SAKK107 pKa = 9.08RR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84MRR112 pKa = 11.84RR113 pKa = 11.84GKK115 pKa = 10.39INN117 pKa = 3.06

Molecular weight: 12.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6416

0

6416

3727238

51

5367

580.9

62.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.383 ± 0.031

0.934 ± 0.009

5.765 ± 0.02

6.198 ± 0.03

3.188 ± 0.016

7.283 ± 0.031

2.271 ± 0.011

3.597 ± 0.019

4.102 ± 0.026

9.041 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.668 ± 0.009

2.67 ± 0.012

7.023 ± 0.039

3.83 ± 0.023

6.741 ± 0.024

8.852 ± 0.047

5.91 ± 0.018

6.085 ± 0.021

1.208 ± 0.009

2.251 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski