Arthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) (Microsporum gypseum) 
Average proteome isoelectric point is 6.72 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 8921 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|E4UR97|E4UR97_ARTGP NADH:ubiquinone reductase (non-electrogenic) OS=Arthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) OX=535722 GN=MGYG_03165 PE=3 SV=1 
MM1 pKa = 7.61  RR2 pKa = 11.84  FSFVSLILASAAFVSAAKK20 pKa = 9.78  MGDD23 pKa = 3.02  IIGTVNTDD31 pKa = 3.66  DD32 pKa = 3.79  NCASEE37 pKa = 5.73  GVDD40 pKa = 5.39  LLDD43 pKa = 3.85  STCTASSKK51 pKa = 10.93  GSINVTADD59 pKa = 3.48  TILCFAYY66 pKa = 9.63  PDD68 pKa = 4.35  SEE70 pKa = 4.7  CKK72 pKa = 10.06  TSTTTPDD79 pKa = 2.85  GVYY82 pKa = 10.44  LEE84 pKa = 4.88  KK85 pKa = 11.01  GCNVDD90 pKa = 3.7  EE91 pKa = 4.99  KK92 pKa = 11.18  LSEE95 pKa = 4.6  DD96 pKa = 4.22  DD97 pKa = 5.41  VSLLCYY103 pKa = 10.43  AA104 pKa = 4.95   
 Molecular weight: 10.91 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.773 
IPC2_protein 3.859 
IPC_protein 3.808 
Toseland    3.592 
ProMoST     3.999 
Dawson      3.821 
Bjellqvist  3.973 
Wikipedia   3.783 
Rodwell     3.643 
Grimsley    3.503 
Solomon     3.795 
Lehninger   3.757 
Nozaki      3.935 
DTASelect   4.19 
Thurlkill   3.668 
EMBOSS      3.783 
Sillero     3.935 
Patrickios  0.54 
IPC_peptide 3.795 
IPC2_peptide  3.91 
IPC2.peptide.svr19  3.829 
 Protein with the highest isoelectric point: 
>tr|E5QZN5|E5QZN5_ARTGP Uncharacterized protein OS=Arthroderma gypseum (strain ATCC MYA-4604 / CBS 118893) OX=535722 GN=MGYG_01222 PE=3 SV=1 
MM1 pKa = 7.53  SPPEE5 pKa = 3.72  PVAQRR10 pKa = 11.84  FRR12 pKa = 11.84  NLVWRR17 pKa = 11.84  EE18 pKa = 3.13  RR19 pKa = 11.84  SLRR22 pKa = 11.84  FTCEE26 pKa = 3.06  NFLRR30 pKa = 11.84  LTVFPSFRR38 pKa = 11.84  GKK40 pKa = 8.14  QTGQAEE46 pKa = 4.58  SVEE49 pKa = 4.37  GEE51 pKa = 4.37  HH52 pKa = 6.03  EE53 pKa = 4.32  NKK55 pKa = 10.29  RR56 pKa = 11.84  ADD58 pKa = 3.37  YY59 pKa = 11.05  GNRR62 pKa = 11.84  KK63 pKa = 5.68  TKK65 pKa = 9.83  RR66 pKa = 11.84  RR67 pKa = 11.84  RR68 pKa = 11.84  RR69 pKa = 11.84  RR70 pKa = 11.84  RR71 pKa = 11.84  NGSKK75 pKa = 8.77  TANFVALRR83 pKa = 11.84  TTLACCAARR92 pKa = 11.84  LLDD95 pKa = 3.63  INSYY99 pKa = 10.95  LDD101 pKa = 3.37  AGRR104 pKa = 11.84  KK105 pKa = 7.14  EE106 pKa = 3.95  SRR108 pKa = 11.84  KK109 pKa = 10.18  AFWGWCSIIKK119 pKa = 10.13  HH120 pKa = 5.36  DD121 pKa = 4.34  TCNKK125 pKa = 5.61  PTPYY129 pKa = 10.97   
 Molecular weight: 15.08 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.351 
IPC2_protein 9.619 
IPC_protein 10.394 
Toseland    10.774 
ProMoST     10.482 
Dawson      10.847 
Bjellqvist  10.57 
Wikipedia   11.067 
Rodwell     11.052 
Grimsley    10.891 
Solomon     10.994 
Lehninger   10.965 
Nozaki      10.774 
DTASelect   10.555 
Thurlkill   10.774 
EMBOSS      11.184 
Sillero     10.789 
Patrickios  10.774 
IPC_peptide 11.008 
IPC2_peptide  9.794 
IPC2.peptide.svr19  8.62 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        8921 
 
        
        0
 
        
        8921 
         
        4257366
 
        35
 
        9330
 
        477.2
 
        52.89
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        8.201 ± 0.026
1.263 ± 0.011
 
        5.596 ± 0.019
6.268 ± 0.025
 
        3.623 ± 0.018
6.669 ± 0.02
 
        2.419 ± 0.012
5.153 ± 0.02
       
        5.153 ± 0.023
8.847 ± 0.029
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.134 ± 0.01
3.803 ± 0.013
 
        6.143 ± 0.03
4.102 ± 0.022
 
        6.146 ± 0.022
8.669 ± 0.032
 
        5.903 ± 0.018
5.831 ± 0.017
       
        1.327 ± 0.009
2.75 ± 0.013
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here