Amino acid dipepetide frequency for Streptococcus phage Javan73

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.636AlaAla: 3.636 ± 0.812
0.606AlaCys: 0.606 ± 0.215
4.646AlaAsp: 4.646 ± 0.598
5.555AlaGlu: 5.555 ± 0.51
1.919AlaPhe: 1.919 ± 0.409
3.131AlaGly: 3.131 ± 0.591
0.505AlaHis: 0.505 ± 0.241
4.343AlaIle: 4.343 ± 0.551
5.757AlaLys: 5.757 ± 0.836
6.666AlaLeu: 6.666 ± 0.994
2.222AlaMet: 2.222 ± 0.49
3.03AlaAsn: 3.03 ± 0.759
1.515AlaPro: 1.515 ± 0.365
1.919AlaGln: 1.919 ± 0.381
2.424AlaArg: 2.424 ± 0.547
4.747AlaSer: 4.747 ± 0.744
2.727AlaThr: 2.727 ± 0.435
4.949AlaVal: 4.949 ± 0.918
0.505AlaTrp: 0.505 ± 0.183
2.525AlaTyr: 2.525 ± 0.656
0.0AlaXaa: 0.0 ± 0.0
Cys
0.303CysAla: 0.303 ± 0.209
0.0CysCys: 0.0 ± 0.0
0.404CysAsp: 0.404 ± 0.197
0.909CysGlu: 0.909 ± 0.295
0.202CysPhe: 0.202 ± 0.15
0.101CysGly: 0.101 ± 0.098
0.101CysHis: 0.101 ± 0.084
0.606CysIle: 0.606 ± 0.303
0.505CysLys: 0.505 ± 0.221
0.505CysLeu: 0.505 ± 0.233
0.0CysMet: 0.0 ± 0.0
0.303CysAsn: 0.303 ± 0.176
0.202CysPro: 0.202 ± 0.152
0.202CysGln: 0.202 ± 0.131
0.101CysArg: 0.101 ± 0.135
0.303CysSer: 0.303 ± 0.173
0.404CysThr: 0.404 ± 0.213
0.505CysVal: 0.505 ± 0.23
0.0CysTrp: 0.0 ± 0.0
0.404CysTyr: 0.404 ± 0.24
0.0CysXaa: 0.0 ± 0.0
Asp
2.929AspAla: 2.929 ± 0.523
0.404AspCys: 0.404 ± 0.203
3.333AspAsp: 3.333 ± 0.763
5.555AspGlu: 5.555 ± 0.781
4.242AspPhe: 4.242 ± 0.514
4.646AspGly: 4.646 ± 0.863
0.202AspHis: 0.202 ± 0.143
5.656AspIle: 5.656 ± 0.801
5.757AspLys: 5.757 ± 0.591
5.05AspLeu: 5.05 ± 0.578
2.727AspMet: 2.727 ± 0.589
3.131AspAsn: 3.131 ± 0.564
1.616AspPro: 1.616 ± 0.417
1.313AspGln: 1.313 ± 0.364
1.717AspArg: 1.717 ± 0.368
3.03AspSer: 3.03 ± 0.482
2.525AspThr: 2.525 ± 0.55
3.03AspVal: 3.03 ± 0.58
0.707AspTrp: 0.707 ± 0.212
3.838AspTyr: 3.838 ± 0.682
0.0AspXaa: 0.0 ± 0.0
Glu
3.939GluAla: 3.939 ± 0.642
0.404GluCys: 0.404 ± 0.199
3.131GluAsp: 3.131 ± 0.61
7.272GluGlu: 7.272 ± 1.149
2.727GluPhe: 2.727 ± 0.539
3.737GluGly: 3.737 ± 0.526
1.212GluHis: 1.212 ± 0.352
7.474GluIle: 7.474 ± 0.93
6.06GluLys: 6.06 ± 0.981
8.08GluLeu: 8.08 ± 1.138
2.323GluMet: 2.323 ± 0.549
5.05GluAsn: 5.05 ± 0.696
1.414GluPro: 1.414 ± 0.428
3.434GluGln: 3.434 ± 0.423
3.232GluArg: 3.232 ± 0.639
3.939GluSer: 3.939 ± 0.576
4.545GluThr: 4.545 ± 0.581
5.858GluVal: 5.858 ± 0.688
1.111GluTrp: 1.111 ± 0.289
2.323GluTyr: 2.323 ± 0.49
0.0GluXaa: 0.0 ± 0.0
Phe
2.525PheAla: 2.525 ± 0.436
0.202PheCys: 0.202 ± 0.165
4.04PheAsp: 4.04 ± 0.459
3.636PheGlu: 3.636 ± 0.725
1.01PhePhe: 1.01 ± 0.443
3.636PheGly: 3.636 ± 0.58
0.606PheHis: 0.606 ± 0.231
3.535PheIle: 3.535 ± 0.639
3.737PheLys: 3.737 ± 0.616
3.131PheLeu: 3.131 ± 0.455
1.111PheMet: 1.111 ± 0.419
2.828PheAsn: 2.828 ± 0.501
1.616PhePro: 1.616 ± 0.452
1.818PheGln: 1.818 ± 0.508
1.313PheArg: 1.313 ± 0.301
2.222PheSer: 2.222 ± 0.388
1.818PheThr: 1.818 ± 0.324
2.424PheVal: 2.424 ± 0.549
0.404PheTrp: 0.404 ± 0.222
2.222PheTyr: 2.222 ± 0.504
0.0PheXaa: 0.0 ± 0.0
Gly
3.333GlyAla: 3.333 ± 0.791
0.505GlyCys: 0.505 ± 0.221
4.04GlyAsp: 4.04 ± 0.648
3.333GlyGlu: 3.333 ± 0.527
2.121GlyPhe: 2.121 ± 0.493
3.333GlyGly: 3.333 ± 0.631
0.808GlyHis: 0.808 ± 0.336
4.848GlyIle: 4.848 ± 0.663
5.454GlyLys: 5.454 ± 0.575
4.646GlyLeu: 4.646 ± 0.533
1.515GlyMet: 1.515 ± 0.353
4.343GlyAsn: 4.343 ± 0.811
0.707GlyPro: 0.707 ± 0.225
2.323GlyGln: 2.323 ± 0.412
2.424GlyArg: 2.424 ± 0.516
3.838GlySer: 3.838 ± 0.565
3.737GlyThr: 3.737 ± 0.564
3.737GlyVal: 3.737 ± 0.883
0.707GlyTrp: 0.707 ± 0.314
3.03GlyTyr: 3.03 ± 0.621
0.0GlyXaa: 0.0 ± 0.0
His
1.111HisAla: 1.111 ± 0.367
0.303HisCys: 0.303 ± 0.148
1.313HisAsp: 1.313 ± 0.365
1.212HisGlu: 1.212 ± 0.391
0.808HisPhe: 0.808 ± 0.198
0.505HisGly: 0.505 ± 0.213
0.303HisHis: 0.303 ± 0.158
1.818HisIle: 1.818 ± 0.397
0.909HisLys: 0.909 ± 0.288
1.313HisLeu: 1.313 ± 0.509
0.202HisMet: 0.202 ± 0.163
1.01HisAsn: 1.01 ± 0.288
0.404HisPro: 0.404 ± 0.186
0.909HisGln: 0.909 ± 0.297
0.303HisArg: 0.303 ± 0.177
1.01HisSer: 1.01 ± 0.378
0.707HisThr: 0.707 ± 0.238
0.808HisVal: 0.808 ± 0.299
0.303HisTrp: 0.303 ± 0.165
0.808HisTyr: 0.808 ± 0.234
0.0HisXaa: 0.0 ± 0.0
Ile
5.353IleAla: 5.353 ± 0.737
0.404IleCys: 0.404 ± 0.24
6.06IleAsp: 6.06 ± 0.733
4.646IleGlu: 4.646 ± 0.53
3.232IlePhe: 3.232 ± 0.521
3.737IleGly: 3.737 ± 0.635
1.717IleHis: 1.717 ± 0.412
5.05IleIle: 5.05 ± 0.821
7.171IleLys: 7.171 ± 1.119
5.656IleLeu: 5.656 ± 0.903
2.424IleMet: 2.424 ± 0.467
4.242IleAsn: 4.242 ± 0.768
2.02IlePro: 2.02 ± 0.293
2.929IleGln: 2.929 ± 0.448
1.717IleArg: 1.717 ± 0.415
5.252IleSer: 5.252 ± 0.783
4.646IleThr: 4.646 ± 0.66
6.161IleVal: 6.161 ± 0.836
1.212IleTrp: 1.212 ± 0.781
1.818IleTyr: 1.818 ± 0.337
0.0IleXaa: 0.0 ± 0.0
Lys
5.555LysAla: 5.555 ± 0.852
0.505LysCys: 0.505 ± 0.204
4.343LysAsp: 4.343 ± 0.766
6.969LysGlu: 6.969 ± 0.896
3.434LysPhe: 3.434 ± 0.569
4.545LysGly: 4.545 ± 0.597
1.313LysHis: 1.313 ± 0.526
5.656LysIle: 5.656 ± 0.713
6.565LysLys: 6.565 ± 0.852
7.373LysLeu: 7.373 ± 0.89
2.424LysMet: 2.424 ± 0.606
6.161LysAsn: 6.161 ± 0.852
2.525LysPro: 2.525 ± 0.436
3.333LysGln: 3.333 ± 0.601
4.646LysArg: 4.646 ± 0.726
5.656LysSer: 5.656 ± 0.819
6.06LysThr: 6.06 ± 0.876
5.151LysVal: 5.151 ± 0.676
0.909LysTrp: 0.909 ± 0.298
2.222LysTyr: 2.222 ± 0.507
0.0LysXaa: 0.0 ± 0.0
Leu
6.262LeuAla: 6.262 ± 0.748
0.303LeuCys: 0.303 ± 0.184
4.949LeuAsp: 4.949 ± 0.79
7.676LeuGlu: 7.676 ± 0.94
4.444LeuPhe: 4.444 ± 0.819
5.858LeuGly: 5.858 ± 0.677
1.414LeuHis: 1.414 ± 0.499
6.06LeuIle: 6.06 ± 0.909
7.474LeuLys: 7.474 ± 0.606
6.262LeuLeu: 6.262 ± 0.781
3.131LeuMet: 3.131 ± 0.522
5.454LeuAsn: 5.454 ± 0.551
2.222LeuPro: 2.222 ± 0.475
2.828LeuGln: 2.828 ± 0.593
3.333LeuArg: 3.333 ± 0.698
4.949LeuSer: 4.949 ± 0.905
5.959LeuThr: 5.959 ± 0.711
3.232LeuVal: 3.232 ± 0.539
0.909LeuTrp: 0.909 ± 0.338
3.03LeuTyr: 3.03 ± 0.614
0.0LeuXaa: 0.0 ± 0.0
Met
2.02MetAla: 2.02 ± 0.558
0.101MetCys: 0.101 ± 0.084
1.313MetAsp: 1.313 ± 0.394
2.121MetGlu: 2.121 ± 0.526
1.212MetPhe: 1.212 ± 0.387
1.616MetGly: 1.616 ± 0.308
0.303MetHis: 0.303 ± 0.203
1.414MetIle: 1.414 ± 0.479
2.323MetLys: 2.323 ± 0.427
1.818MetLeu: 1.818 ± 0.46
0.707MetMet: 0.707 ± 0.286
1.818MetAsn: 1.818 ± 0.384
0.505MetPro: 0.505 ± 0.228
1.212MetGln: 1.212 ± 0.333
1.212MetArg: 1.212 ± 0.405
2.525MetSer: 2.525 ± 0.506
2.525MetThr: 2.525 ± 0.618
2.02MetVal: 2.02 ± 0.377
0.202MetTrp: 0.202 ± 0.156
1.01MetTyr: 1.01 ± 0.364
0.0MetXaa: 0.0 ± 0.0
Asn
3.939AsnAla: 3.939 ± 0.648
0.303AsnCys: 0.303 ± 0.231
3.636AsnAsp: 3.636 ± 0.69
4.343AsnGlu: 4.343 ± 0.6
2.727AsnPhe: 2.727 ± 0.447
4.444AsnGly: 4.444 ± 0.736
1.313AsnHis: 1.313 ± 0.391
3.737AsnIle: 3.737 ± 0.719
4.141AsnLys: 4.141 ± 0.601
5.555AsnLeu: 5.555 ± 0.732
1.212AsnMet: 1.212 ± 0.446
2.626AsnAsn: 2.626 ± 0.398
2.626AsnPro: 2.626 ± 0.704
2.828AsnGln: 2.828 ± 0.505
2.828AsnArg: 2.828 ± 0.613
4.444AsnSer: 4.444 ± 0.704
4.444AsnThr: 4.444 ± 0.883
3.636AsnVal: 3.636 ± 0.681
0.808AsnTrp: 0.808 ± 0.263
1.717AsnTyr: 1.717 ± 0.396
0.0AsnXaa: 0.0 ± 0.0
Pro
1.414ProAla: 1.414 ± 0.371
0.202ProCys: 0.202 ± 0.141
2.222ProAsp: 2.222 ± 0.693
1.818ProGlu: 1.818 ± 0.4
1.414ProPhe: 1.414 ± 0.431
0.707ProGly: 0.707 ± 0.222
0.606ProHis: 0.606 ± 0.257
3.131ProIle: 3.131 ± 0.568
2.727ProLys: 2.727 ± 0.477
2.121ProLeu: 2.121 ± 0.432
0.404ProMet: 0.404 ± 0.182
0.808ProAsn: 0.808 ± 0.321
0.202ProPro: 0.202 ± 0.148
1.515ProGln: 1.515 ± 0.354
1.212ProArg: 1.212 ± 0.415
0.909ProSer: 0.909 ± 0.232
1.818ProThr: 1.818 ± 0.359
1.515ProVal: 1.515 ± 0.338
0.404ProTrp: 0.404 ± 0.17
1.313ProTyr: 1.313 ± 0.385
0.0ProXaa: 0.0 ± 0.0
Gln
4.04GlnAla: 4.04 ± 0.775
0.404GlnCys: 0.404 ± 0.197
2.02GlnAsp: 2.02 ± 0.419
3.131GlnGlu: 3.131 ± 0.587
1.414GlnPhe: 1.414 ± 0.406
1.717GlnGly: 1.717 ± 0.428
0.707GlnHis: 0.707 ± 0.231
2.727GlnIle: 2.727 ± 0.435
4.04GlnLys: 4.04 ± 0.616
2.828GlnLeu: 2.828 ± 0.541
1.01GlnMet: 1.01 ± 0.342
2.626GlnAsn: 2.626 ± 0.44
1.313GlnPro: 1.313 ± 0.333
1.313GlnGln: 1.313 ± 0.37
1.919GlnArg: 1.919 ± 0.485
2.424GlnSer: 2.424 ± 0.505
2.222GlnThr: 2.222 ± 0.497
2.222GlnVal: 2.222 ± 0.484
0.303GlnTrp: 0.303 ± 0.213
1.414GlnTyr: 1.414 ± 0.316
0.0GlnXaa: 0.0 ± 0.0
Arg
2.727ArgAla: 2.727 ± 0.503
0.0ArgCys: 0.0 ± 0.0
2.323ArgAsp: 2.323 ± 0.599
3.737ArgGlu: 3.737 ± 0.561
1.515ArgPhe: 1.515 ± 0.446
1.818ArgGly: 1.818 ± 0.428
1.111ArgHis: 1.111 ± 0.26
2.525ArgIle: 2.525 ± 0.442
3.636ArgLys: 3.636 ± 0.748
3.939ArgLeu: 3.939 ± 0.755
1.01ArgMet: 1.01 ± 0.258
3.03ArgAsn: 3.03 ± 0.505
1.616ArgPro: 1.616 ± 0.394
1.919ArgGln: 1.919 ± 0.422
1.717ArgArg: 1.717 ± 0.42
2.02ArgSer: 2.02 ± 0.443
2.323ArgThr: 2.323 ± 0.556
1.818ArgVal: 1.818 ± 0.429
0.303ArgTrp: 0.303 ± 0.154
1.717ArgTyr: 1.717 ± 0.428
0.0ArgXaa: 0.0 ± 0.0
Ser
3.333SerAla: 3.333 ± 0.582
0.303SerCys: 0.303 ± 0.202
3.636SerAsp: 3.636 ± 0.578
3.838SerGlu: 3.838 ± 0.481
3.737SerPhe: 3.737 ± 0.573
3.838SerGly: 3.838 ± 0.65
0.808SerHis: 0.808 ± 0.246
4.141SerIle: 4.141 ± 0.823
4.747SerLys: 4.747 ± 0.912
5.05SerLeu: 5.05 ± 0.561
1.818SerMet: 1.818 ± 0.517
4.242SerAsn: 4.242 ± 0.697
1.717SerPro: 1.717 ± 0.488
3.838SerGln: 3.838 ± 0.702
2.424SerArg: 2.424 ± 0.375
4.242SerSer: 4.242 ± 0.814
3.03SerThr: 3.03 ± 0.729
3.131SerVal: 3.131 ± 0.453
0.808SerTrp: 0.808 ± 0.225
2.02SerTyr: 2.02 ± 0.437
0.0SerXaa: 0.0 ± 0.0
Thr
4.545ThrAla: 4.545 ± 0.744
0.202ThrCys: 0.202 ± 0.136
3.939ThrAsp: 3.939 ± 0.792
3.636ThrGlu: 3.636 ± 0.823
2.323ThrPhe: 2.323 ± 0.526
3.939ThrGly: 3.939 ± 0.581
0.909ThrHis: 0.909 ± 0.324
5.454ThrIle: 5.454 ± 0.962
5.757ThrLys: 5.757 ± 0.705
6.06ThrLeu: 6.06 ± 0.792
1.111ThrMet: 1.111 ± 0.35
2.222ThrAsn: 2.222 ± 0.519
1.313ThrPro: 1.313 ± 0.431
2.121ThrGln: 2.121 ± 0.506
2.727ThrArg: 2.727 ± 0.469
2.222ThrSer: 2.222 ± 0.415
2.929ThrThr: 2.929 ± 0.756
4.343ThrVal: 4.343 ± 0.596
0.707ThrTrp: 0.707 ± 0.323
2.525ThrTyr: 2.525 ± 0.48
0.0ThrXaa: 0.0 ± 0.0
Val
3.636ValAla: 3.636 ± 0.565
0.202ValCys: 0.202 ± 0.142
3.636ValAsp: 3.636 ± 0.519
4.747ValGlu: 4.747 ± 0.627
2.121ValPhe: 2.121 ± 0.347
4.545ValGly: 4.545 ± 0.651
1.414ValHis: 1.414 ± 0.35
4.343ValIle: 4.343 ± 0.537
5.151ValLys: 5.151 ± 0.75
5.252ValLeu: 5.252 ± 0.787
1.515ValMet: 1.515 ± 0.436
3.939ValAsn: 3.939 ± 0.555
1.515ValPro: 1.515 ± 0.419
1.515ValGln: 1.515 ± 0.323
2.222ValArg: 2.222 ± 0.505
4.242ValSer: 4.242 ± 0.437
4.343ValThr: 4.343 ± 0.558
3.838ValVal: 3.838 ± 0.572
0.808ValTrp: 0.808 ± 0.297
2.121ValTyr: 2.121 ± 0.444
0.0ValXaa: 0.0 ± 0.0
Trp
0.505TrpAla: 0.505 ± 0.177
0.303TrpCys: 0.303 ± 0.166
0.707TrpAsp: 0.707 ± 0.231
0.606TrpGlu: 0.606 ± 0.368
0.707TrpPhe: 0.707 ± 0.182
0.808TrpGly: 0.808 ± 0.238
0.202TrpHis: 0.202 ± 0.144
0.404TrpIle: 0.404 ± 0.246
0.909TrpLys: 0.909 ± 0.284
0.909TrpLeu: 0.909 ± 0.256
0.101TrpMet: 0.101 ± 0.101
1.919TrpAsn: 1.919 ± 1.04
0.101TrpPro: 0.101 ± 0.113
0.707TrpGln: 0.707 ± 0.258
0.505TrpArg: 0.505 ± 0.221
0.808TrpSer: 0.808 ± 0.274
0.101TrpThr: 0.101 ± 0.088
0.505TrpVal: 0.505 ± 0.207
0.101TrpTrp: 0.101 ± 0.088
0.404TrpTyr: 0.404 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.222TyrAla: 2.222 ± 0.442
0.404TyrCys: 0.404 ± 0.238
1.717TyrAsp: 1.717 ± 0.429
2.525TyrGlu: 2.525 ± 0.481
2.525TyrPhe: 2.525 ± 0.502
2.121TyrGly: 2.121 ± 0.442
0.505TyrHis: 0.505 ± 0.228
2.525TyrIle: 2.525 ± 0.524
2.525TyrLys: 2.525 ± 0.596
3.535TyrLeu: 3.535 ± 0.467
0.909TyrMet: 0.909 ± 0.289
2.525TyrAsn: 2.525 ± 0.468
1.313TyrPro: 1.313 ± 0.318
1.818TyrGln: 1.818 ± 0.429
2.828TyrArg: 2.828 ± 0.454
2.02TyrSer: 2.02 ± 0.48
2.121TyrThr: 2.121 ± 0.476
2.222TyrVal: 2.222 ± 0.432
0.101TyrTrp: 0.101 ± 0.101
1.818TyrTyr: 1.818 ± 0.482
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (9902 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski