Naegleria gruberi (Amoeba)

Taxonomy: cellular organisms; Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia; Vahlkampfiidae; Naegleria

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15636 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2VM26|D2VM26_NAEGR Predicted protein OS=Naegleria gruberi OX=5762 GN=NAEGRDRAFT_50681 PE=3 SV=1
MM1 pKa = 7.29LTLIMVDD8 pKa = 3.51EE9 pKa = 4.58VDD11 pKa = 4.24DD12 pKa = 4.14GRR14 pKa = 11.84IIDD17 pKa = 3.63TSNCVRR23 pKa = 11.84TTNDD27 pKa = 2.94GEE29 pKa = 4.38AFSPLTALMNCEE41 pKa = 4.05SMLAKK46 pKa = 9.09QTFNDD51 pKa = 3.99CCSDD55 pKa = 3.55DD56 pKa = 4.35KK57 pKa = 11.03IYY59 pKa = 11.01EE60 pKa = 4.27VIRR63 pKa = 11.84KK64 pKa = 8.98GLEE67 pKa = 3.4FDD69 pKa = 3.46EE70 pKa = 4.6QFISEE75 pKa = 4.4EE76 pKa = 4.41DD77 pKa = 3.12SDD79 pKa = 5.01ADD81 pKa = 3.8YY82 pKa = 11.41SCEE85 pKa = 4.02SSSEE89 pKa = 4.21DD90 pKa = 3.27EE91 pKa = 4.71TEE93 pKa = 5.67DD94 pKa = 3.56EE95 pKa = 5.16DD96 pKa = 4.88SDD98 pKa = 5.24AEE100 pKa = 4.29EE101 pKa = 4.34EE102 pKa = 4.61CPLFLLDD109 pKa = 3.7SSFYY113 pKa = 10.92GKK115 pKa = 10.41RR116 pKa = 11.84NRR118 pKa = 11.84CEE120 pKa = 3.53YY121 pKa = 10.96DD122 pKa = 3.06DD123 pKa = 5.21YY124 pKa = 11.78YY125 pKa = 11.67SEE127 pKa = 6.5DD128 pKa = 3.75DD129 pKa = 4.84FEE131 pKa = 6.82DD132 pKa = 3.63DD133 pKa = 4.36RR134 pKa = 11.84KK135 pKa = 8.63KK136 pKa = 9.38TRR138 pKa = 11.84YY139 pKa = 9.28

Molecular weight: 16.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2VFD1|D2VFD1_NAEGR Predicted protein OS=Naegleria gruberi OX=5762 GN=NAEGRDRAFT_79743 PE=4 SV=1
MM1 pKa = 7.53NKK3 pKa = 8.6TLLFALAMLFCMVAFVSAGPSASAEE28 pKa = 4.09TLAATTLCNTLGNKK42 pKa = 8.19TKK44 pKa = 10.78GCVKK48 pKa = 10.52SSRR51 pKa = 11.84KK52 pKa = 9.06LLKK55 pKa = 10.14RR56 pKa = 11.84VPKK59 pKa = 10.21NKK61 pKa = 9.17RR62 pKa = 11.84RR63 pKa = 11.84QVKK66 pKa = 10.16RR67 pKa = 11.84LLKK70 pKa = 10.15KK71 pKa = 9.93ARR73 pKa = 11.84KK74 pKa = 8.24NARR77 pKa = 11.84KK78 pKa = 9.5AKK80 pKa = 8.9KK81 pKa = 7.58QQRR84 pKa = 11.84KK85 pKa = 7.87LRR87 pKa = 11.84KK88 pKa = 9.23LRR90 pKa = 11.84RR91 pKa = 11.84CAKK94 pKa = 9.6KK95 pKa = 10.45AKK97 pKa = 9.35SLKK100 pKa = 9.85KK101 pKa = 9.96RR102 pKa = 11.84RR103 pKa = 11.84QCRR106 pKa = 11.84RR107 pKa = 11.84ALKK110 pKa = 10.46KK111 pKa = 10.18VVKK114 pKa = 9.86KK115 pKa = 10.26IKK117 pKa = 9.15KK118 pKa = 8.55ARR120 pKa = 11.84RR121 pKa = 11.84AARR124 pKa = 11.84RR125 pKa = 11.84AAKK128 pKa = 9.96KK129 pKa = 9.54AAKK132 pKa = 9.55KK133 pKa = 9.74ARR135 pKa = 11.84KK136 pKa = 9.01ARR138 pKa = 11.84RR139 pKa = 11.84SARR142 pKa = 11.84RR143 pKa = 11.84AARR146 pKa = 11.84RR147 pKa = 11.84KK148 pKa = 9.39ARR150 pKa = 11.84KK151 pKa = 9.0ALKK154 pKa = 9.69KK155 pKa = 10.36AKK157 pKa = 9.97KK158 pKa = 9.32SLKK161 pKa = 9.64KK162 pKa = 10.2RR163 pKa = 11.84RR164 pKa = 11.84ALKK167 pKa = 9.65RR168 pKa = 11.84AVRR171 pKa = 11.84KK172 pKa = 9.38GRR174 pKa = 11.84KK175 pKa = 8.77FGRR178 pKa = 11.84KK179 pKa = 5.23VRR181 pKa = 11.84KK182 pKa = 9.03VRR184 pKa = 11.84KK185 pKa = 8.87CKK187 pKa = 10.08RR188 pKa = 11.84SSSRR192 pKa = 11.84KK193 pKa = 5.65TRR195 pKa = 11.84RR196 pKa = 11.84AAKK199 pKa = 9.95RR200 pKa = 11.84RR201 pKa = 11.84VKK203 pKa = 10.56KK204 pKa = 10.3AVKK207 pKa = 9.75KK208 pKa = 10.58ALRR211 pKa = 11.84KK212 pKa = 9.46LKK214 pKa = 10.48KK215 pKa = 9.55QLKK218 pKa = 8.78KK219 pKa = 10.47ARR221 pKa = 11.84KK222 pKa = 7.18VAKK225 pKa = 10.01KK226 pKa = 9.73AAKK229 pKa = 9.51KK230 pKa = 9.64ARR232 pKa = 11.84KK233 pKa = 9.15ARR235 pKa = 11.84RR236 pKa = 11.84LRR238 pKa = 11.84RR239 pKa = 11.84RR240 pKa = 11.84PARR243 pKa = 11.84KK244 pKa = 9.01CRR246 pKa = 11.84RR247 pKa = 11.84VSKK250 pKa = 10.6KK251 pKa = 10.1SIKK254 pKa = 9.78RR255 pKa = 11.84AVKK258 pKa = 9.86RR259 pKa = 11.84VVKK262 pKa = 10.35RR263 pKa = 11.84AVKK266 pKa = 10.22KK267 pKa = 9.78ATLKK271 pKa = 10.47LRR273 pKa = 11.84KK274 pKa = 8.74QLKK277 pKa = 8.94KK278 pKa = 10.68AKK280 pKa = 9.69KK281 pKa = 8.12QVKK284 pKa = 9.35KK285 pKa = 10.48ARR287 pKa = 11.84KK288 pKa = 7.15VAKK291 pKa = 10.09KK292 pKa = 10.02AVKK295 pKa = 9.91KK296 pKa = 10.19ARR298 pKa = 11.84KK299 pKa = 8.67VVKK302 pKa = 9.88KK303 pKa = 10.49VKK305 pKa = 8.57KK306 pKa = 6.95TCRR309 pKa = 11.84RR310 pKa = 11.84KK311 pKa = 9.85VKK313 pKa = 10.32KK314 pKa = 9.69AAKK317 pKa = 9.48KK318 pKa = 7.9IAKK321 pKa = 9.33RR322 pKa = 11.84RR323 pKa = 11.84SAAKK327 pKa = 10.08KK328 pKa = 9.92VVLKK332 pKa = 10.68KK333 pKa = 10.44AAAKK337 pKa = 10.19KK338 pKa = 10.12LNKK341 pKa = 9.48VVNKK345 pKa = 10.03LLKK348 pKa = 10.34LKK350 pKa = 10.53KK351 pKa = 10.22KK352 pKa = 9.94LGKK355 pKa = 10.02RR356 pKa = 11.84FNPKK360 pKa = 9.23KK361 pKa = 10.08HH362 pKa = 5.84IPKK365 pKa = 10.03KK366 pKa = 8.66VRR368 pKa = 11.84KK369 pKa = 8.69VLRR372 pKa = 11.84KK373 pKa = 9.64LRR375 pKa = 11.84ISAKK379 pKa = 9.74CVKK382 pKa = 9.74KK383 pKa = 10.78VKK385 pKa = 10.21KK386 pKa = 9.62AAKK389 pKa = 8.88KK390 pKa = 9.66VKK392 pKa = 9.95VSKK395 pKa = 10.93ALLNKK400 pKa = 9.26LRR402 pKa = 11.84KK403 pKa = 9.59VSSKK407 pKa = 10.24CRR409 pKa = 11.84KK410 pKa = 9.07

Molecular weight: 47.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15634

2

15636

7851348

51

6497

502.1

57.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.376 ± 0.016

1.76 ± 0.019

5.307 ± 0.012

6.861 ± 0.026

4.818 ± 0.014

4.705 ± 0.02

2.08 ± 0.009

7.119 ± 0.015

7.607 ± 0.023

9.111 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.297 ± 0.008

6.7 ± 0.02

3.764 ± 0.013

4.252 ± 0.014

3.744 ± 0.011

9.397 ± 0.025

6.01 ± 0.018

5.645 ± 0.014

0.8 ± 0.005

3.645 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski