Amino acid dipepetide frequency for Naegleria gruberi (Amoeba)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.414AlaAla: 2.414 ± 0.023	0.854AlaCys: 0.854 ± 0.011	2.011AlaAsp: 2.011 ± 0.018	2.416AlaGlu: 2.416 ± 0.022	2.158AlaPhe: 2.158 ± 0.017	2.054AlaGly: 2.054 ± 0.021	0.834AlaHis: 0.834 ± 0.011	3.368AlaIle: 3.368 ± 0.024	3.352AlaLys: 3.352 ± 0.025	4.359AlaLeu: 4.359 ± 0.028	1.018AlaMet: 1.018 ± 0.011	2.67AlaAsn: 2.67 ± 0.018	1.543AlaPro: 1.543 ± 0.015	1.695AlaGln: 1.695 ± 0.016	1.575AlaArg: 1.575 ± 0.016	4.192AlaSer: 4.192 ± 0.026	2.975AlaThr: 2.975 ± 0.024	2.605AlaVal: 2.605 ± 0.019	0.31AlaTrp: 0.31 ± 0.007	1.359AlaTyr: 1.359 ± 0.014	0.0AlaXaa: 0.0 ± 0.0
Cys	0.737CysAla: 0.737 ± 0.012	0.432CysCys: 0.432 ± 0.01	0.919CysAsp: 0.919 ± 0.017	1.086CysGlu: 1.086 ± 0.02	1.004CysPhe: 1.004 ± 0.018	1.017CysGly: 1.017 ± 0.015	0.337CysHis: 0.337 ± 0.007	1.19CysIle: 1.19 ± 0.016	1.306CysLys: 1.306 ± 0.015	1.586CysLeu: 1.586 ± 0.016	0.353CysMet: 0.353 ± 0.007	1.14CysAsn: 1.14 ± 0.03	0.584CysPro: 0.584 ± 0.011	0.667CysGln: 0.667 ± 0.017	0.569CysArg: 0.569 ± 0.009	1.806CysSer: 1.806 ± 0.045	0.953CysThr: 0.953 ± 0.018	1.045CysVal: 1.045 ± 0.015	0.152CysTrp: 0.152 ± 0.005	0.712CysTyr: 0.712 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	2.119AspAla: 2.119 ± 0.021	0.965AspCys: 0.965 ± 0.017	3.74AspAsp: 3.74 ± 0.034	4.847AspGlu: 4.847 ± 0.033	2.833AspPhe: 2.833 ± 0.02	2.596AspGly: 2.596 ± 0.023	1.062AspHis: 1.062 ± 0.013	3.889AspIle: 3.889 ± 0.021	3.275AspLys: 3.275 ± 0.024	5.027AspLeu: 5.027 ± 0.027	1.247AspMet: 1.247 ± 0.013	3.046AspAsn: 3.046 ± 0.02	1.813AspPro: 1.813 ± 0.017	1.927AspGln: 1.927 ± 0.017	1.949AspArg: 1.949 ± 0.022	4.601AspSer: 4.601 ± 0.029	2.53AspThr: 2.53 ± 0.021	2.897AspVal: 2.897 ± 0.02	0.542AspTrp: 0.542 ± 0.008	2.169AspTyr: 2.169 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	2.613GluAla: 2.613 ± 0.02	1.046GluCys: 1.046 ± 0.015	3.789GluAsp: 3.789 ± 0.027	7.192GluGlu: 7.192 ± 0.062	3.057GluPhe: 3.057 ± 0.02	2.703GluGly: 2.703 ± 0.024	1.303GluHis: 1.303 ± 0.016	4.957GluIle: 4.957 ± 0.035	6.541GluLys: 6.541 ± 0.045	6.179GluLeu: 6.179 ± 0.036	2.024GluMet: 2.024 ± 0.019	4.624GluAsn: 4.624 ± 0.028	1.683GluPro: 1.683 ± 0.019	3.007GluGln: 3.007 ± 0.031	2.988GluArg: 2.988 ± 0.025	4.989GluSer: 4.989 ± 0.028	3.793GluThr: 3.793 ± 0.026	2.982GluVal: 2.982 ± 0.023	0.665GluTrp: 0.665 ± 0.01	2.479GluTyr: 2.479 ± 0.016	0.0GluXaa: 0.0 ± 0.0
Phe	2.186PheAla: 2.186 ± 0.018	0.82PheCys: 0.82 ± 0.012	2.953PheAsp: 2.953 ± 0.019	3.393PheGlu: 3.393 ± 0.024	2.158PhePhe: 2.158 ± 0.019	2.817PheGly: 2.817 ± 0.024	1.016PheHis: 1.016 ± 0.013	3.591PheIle: 3.591 ± 0.022	3.536PheLys: 3.536 ± 0.024	4.072PheLeu: 4.072 ± 0.027	1.079PheMet: 1.079 ± 0.012	3.131PheAsn: 3.131 ± 0.023	1.604PhePro: 1.604 ± 0.015	1.625PheGln: 1.625 ± 0.014	1.647PheArg: 1.647 ± 0.015	4.441PheSer: 4.441 ± 0.027	2.872PheThr: 2.872 ± 0.023	2.987PheVal: 2.987 ± 0.02	0.407PheTrp: 0.407 ± 0.007	1.846PheTyr: 1.846 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	2.297GlyAla: 2.297 ± 0.023	0.808GlyCys: 0.808 ± 0.012	2.589GlyAsp: 2.589 ± 0.025	2.722GlyGlu: 2.722 ± 0.022	2.412GlyPhe: 2.412 ± 0.019	3.303GlyGly: 3.303 ± 0.039	0.913GlyHis: 0.913 ± 0.013	3.365GlyIle: 3.365 ± 0.022	3.602GlyLys: 3.602 ± 0.026	4.006GlyLeu: 4.006 ± 0.036	1.132GlyMet: 1.132 ± 0.013	3.155GlyAsn: 3.155 ± 0.027	1.057GlyPro: 1.057 ± 0.012	1.58GlyGln: 1.58 ± 0.018	1.716GlyArg: 1.716 ± 0.019	4.285GlySer: 4.285 ± 0.037	2.69GlyThr: 2.69 ± 0.029	2.97GlyVal: 2.97 ± 0.021	0.468GlyTrp: 0.468 ± 0.009	1.982GlyTyr: 1.982 ± 0.022	0.0GlyXaa: 0.0 ± 0.0
His	0.892HisAla: 0.892 ± 0.011	0.389HisCys: 0.389 ± 0.007	1.049HisAsp: 1.049 ± 0.012	1.169HisGlu: 1.169 ± 0.014	1.115HisPhe: 1.115 ± 0.014	0.961HisGly: 0.961 ± 0.014	1.044HisHis: 1.044 ± 0.019	1.333HisIle: 1.333 ± 0.013	1.158HisLys: 1.158 ± 0.013	2.109HisLeu: 2.109 ± 0.018	0.401HisMet: 0.401 ± 0.007	1.193HisAsn: 1.193 ± 0.014	0.929HisPro: 0.929 ± 0.011	1.089HisGln: 1.089 ± 0.014	0.788HisArg: 0.788 ± 0.012	1.883HisSer: 1.883 ± 0.017	1.052HisThr: 1.052 ± 0.013	1.208HisVal: 1.208 ± 0.014	0.175HisTrp: 0.175 ± 0.005	0.865HisTyr: 0.865 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	3.486IleAla: 3.486 ± 0.02	1.28IleCys: 1.28 ± 0.017	4.078IleAsp: 4.078 ± 0.021	5.023IleGlu: 5.023 ± 0.031	3.173IlePhe: 3.173 ± 0.023	3.66IleGly: 3.66 ± 0.025	1.559IleHis: 1.559 ± 0.013	5.083IleIle: 5.083 ± 0.033	4.525IleLys: 4.525 ± 0.029	6.518IleLeu: 6.518 ± 0.037	1.448IleMet: 1.448 ± 0.014	3.866IleAsn: 3.866 ± 0.025	3.274IlePro: 3.274 ± 0.024	3.011IleGln: 3.011 ± 0.022	2.713IleArg: 2.713 ± 0.021	6.753IleSer: 6.753 ± 0.041	4.062IleThr: 4.062 ± 0.025	4.615IleVal: 4.615 ± 0.027	0.503IleTrp: 0.503 ± 0.008	2.557IleTyr: 2.557 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	3.128LysAla: 3.128 ± 0.028	1.283LysCys: 1.283 ± 0.021	3.754LysAsp: 3.754 ± 0.029	5.945LysGlu: 5.945 ± 0.041	3.216LysPhe: 3.216 ± 0.022	2.85LysGly: 2.85 ± 0.023	1.609LysHis: 1.609 ± 0.016	5.064LysIle: 5.064 ± 0.027	8.087LysLys: 8.087 ± 0.064	6.979LysLeu: 6.979 ± 0.038	1.731LysMet: 1.731 ± 0.014	4.379LysAsn: 4.379 ± 0.025	2.942LysPro: 2.942 ± 0.051	3.826LysGln: 3.826 ± 0.03	3.505LysArg: 3.505 ± 0.025	6.031LysSer: 6.031 ± 0.037	4.174LysThr: 4.174 ± 0.025	3.98LysVal: 3.98 ± 0.028	0.617LysTrp: 0.617 ± 0.009	2.971LysTyr: 2.971 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	4.239LeuAla: 4.239 ± 0.025	1.456LeuCys: 1.456 ± 0.016	5.004LeuAsp: 5.004 ± 0.027	6.26LeuGlu: 6.26 ± 0.039	4.819LeuPhe: 4.819 ± 0.033	3.885LeuGly: 3.885 ± 0.037	1.777LeuHis: 1.777 ± 0.015	6.219LeuIle: 6.219 ± 0.036	7.475LeuLys: 7.475 ± 0.038	8.844LeuLeu: 8.844 ± 0.04	2.163LeuMet: 2.163 ± 0.015	5.845LeuAsn: 5.845 ± 0.033	3.413LeuPro: 3.413 ± 0.026	3.671LeuGln: 3.671 ± 0.026	3.496LeuArg: 3.496 ± 0.025	8.311LeuSer: 8.311 ± 0.033	5.345LeuThr: 5.345 ± 0.035	5.138LeuVal: 5.138 ± 0.028	0.668LeuTrp: 0.668 ± 0.011	3.084LeuTyr: 3.084 ± 0.02	0.001LeuXaa: 0.001 ± 0.0
Met	1.122MetAla: 1.122 ± 0.012	0.32MetCys: 0.32 ± 0.007	1.317MetAsp: 1.317 ± 0.014	1.647MetGlu: 1.647 ± 0.014	1.052MetPhe: 1.052 ± 0.011	0.998MetGly: 0.998 ± 0.013	0.37MetHis: 0.37 ± 0.007	1.733MetIle: 1.733 ± 0.016	2.259MetLys: 2.259 ± 0.023	1.855MetLeu: 1.855 ± 0.017	0.791MetMet: 0.791 ± 0.015	1.726MetAsn: 1.726 ± 0.015	0.678MetPro: 0.678 ± 0.01	0.79MetGln: 0.79 ± 0.01	0.893MetArg: 0.893 ± 0.014	1.939MetSer: 1.939 ± 0.015	1.235MetThr: 1.235 ± 0.012	1.288MetVal: 1.288 ± 0.012	0.197MetTrp: 0.197 ± 0.005	0.764MetTyr: 0.764 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.593AsnAla: 2.593 ± 0.021	1.26AsnCys: 1.26 ± 0.028	3.491AsnAsp: 3.491 ± 0.026	4.14AsnGlu: 4.14 ± 0.027	2.742AsnPhe: 2.742 ± 0.021	3.59AsnGly: 3.59 ± 0.04	1.47AsnHis: 1.47 ± 0.014	4.946AsnIle: 4.946 ± 0.028	3.407AsnLys: 3.407 ± 0.026	5.696AsnLeu: 5.696 ± 0.033	1.453AsnMet: 1.453 ± 0.014	6.805AsnAsn: 6.805 ± 0.081	2.538AsnPro: 2.538 ± 0.022	3.126AsnGln: 3.126 ± 0.023	2.385AsnArg: 2.385 ± 0.02	6.614AsnSer: 6.614 ± 0.045	4.046AsnThr: 4.046 ± 0.032	3.647AsnVal: 3.647 ± 0.021	0.504AsnTrp: 0.504 ± 0.008	2.549AsnTyr: 2.549 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	1.36ProAla: 1.36 ± 0.017	0.423ProCys: 0.423 ± 0.009	1.377ProAsp: 1.377 ± 0.015	2.141ProGlu: 2.141 ± 0.019	1.855ProPhe: 1.855 ± 0.016	1.157ProGly: 1.157 ± 0.014	0.766ProHis: 0.766 ± 0.012	2.898ProIle: 2.898 ± 0.023	3.062ProLys: 3.062 ± 0.052	3.197ProLeu: 3.197 ± 0.02	0.707ProMet: 0.707 ± 0.01	2.632ProAsn: 2.632 ± 0.022	2.219ProPro: 2.219 ± 0.037	1.786ProGln: 1.786 ± 0.019	1.21ProArg: 1.21 ± 0.015	4.23ProSer: 4.23 ± 0.031	2.983ProThr: 2.983 ± 0.024	2.085ProVal: 2.085 ± 0.022	0.219ProTrp: 0.219 ± 0.005	1.327ProTyr: 1.327 ± 0.014	0.0ProXaa: 0.0 ± 0.0
Gln	1.821GlnAla: 1.821 ± 0.016	0.642GlnCys: 0.642 ± 0.016	1.906GlnAsp: 1.906 ± 0.016	2.739GlnGlu: 2.739 ± 0.028	2.113GlnPhe: 2.113 ± 0.017	1.377GlnGly: 1.377 ± 0.017	1.086GlnHis: 1.086 ± 0.013	2.76GlnIle: 2.76 ± 0.021	2.918GlnLys: 2.918 ± 0.022	4.235GlnLeu: 4.235 ± 0.026	1.037GlnMet: 1.037 ± 0.012	2.476GlnAsn: 2.476 ± 0.019	1.844GlnPro: 1.844 ± 0.023	3.692GlnGln: 3.692 ± 0.05	1.472GlnArg: 1.472 ± 0.016	3.434GlnSer: 3.434 ± 0.027	2.464GlnThr: 2.464 ± 0.021	2.521GlnVal: 2.521 ± 0.019	0.306GlnTrp: 0.306 ± 0.007	1.672GlnTyr: 1.672 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	1.633ArgAla: 1.633 ± 0.015	0.512ArgCys: 0.512 ± 0.008	2.005ArgAsp: 2.005 ± 0.019	2.771ArgGlu: 2.771 ± 0.026	1.814ArgPhe: 1.814 ± 0.015	1.796ArgGly: 1.796 ± 0.02	0.691ArgHis: 0.691 ± 0.008	2.761ArgIle: 2.761 ± 0.022	3.787ArgLys: 3.787 ± 0.031	3.178ArgLeu: 3.178 ± 0.024	1.002ArgMet: 1.002 ± 0.012	2.602ArgAsn: 2.602 ± 0.022	1.116ArgPro: 1.116 ± 0.015	1.325ArgGln: 1.325 ± 0.015	1.96ArgArg: 1.96 ± 0.025	2.754ArgSer: 2.754 ± 0.018	1.812ArgThr: 1.812 ± 0.018	2.259ArgVal: 2.259 ± 0.019	0.304ArgTrp: 0.304 ± 0.006	1.358ArgTyr: 1.358 ± 0.013	0.0ArgXaa: 0.0 ± 0.0
Ser	3.845SerAla: 3.845 ± 0.023	1.529SerCys: 1.529 ± 0.027	4.668SerAsp: 4.668 ± 0.029	5.294SerGlu: 5.294 ± 0.029	4.431SerPhe: 4.431 ± 0.026	4.461SerGly: 4.461 ± 0.037	1.735SerHis: 1.735 ± 0.016	6.6SerIle: 6.6 ± 0.035	6.406SerLys: 6.406 ± 0.031	8.397SerLeu: 8.397 ± 0.042	1.876SerMet: 1.876 ± 0.016	7.105SerAsn: 7.105 ± 0.051	3.801SerPro: 3.801 ± 0.031	3.564SerGln: 3.564 ± 0.024	2.874SerArg: 2.874 ± 0.021	12.421SerSer: 12.421 ± 0.09	6.566SerThr: 6.566 ± 0.045	4.926SerVal: 4.926 ± 0.029	0.614SerTrp: 0.614 ± 0.011	2.855SerTyr: 2.855 ± 0.022	0.001SerXaa: 0.001 ± 0.0
Thr	2.565ThrAla: 2.565 ± 0.02	1.285ThrCys: 1.285 ± 0.032	2.715ThrAsp: 2.715 ± 0.021	3.246ThrGlu: 3.246 ± 0.026	2.919ThrPhe: 2.919 ± 0.022	2.613ThrGly: 2.613 ± 0.025	1.084ThrHis: 1.084 ± 0.012	4.648ThrIle: 4.648 ± 0.03	4.049ThrLys: 4.049 ± 0.024	5.376ThrLeu: 5.376 ± 0.028	1.137ThrMet: 1.137 ± 0.011	4.199ThrAsn: 4.199 ± 0.029	3.034ThrPro: 3.034 ± 0.027	2.226ThrGln: 2.226 ± 0.02	1.983ThrArg: 1.983 ± 0.017	6.273ThrSer: 6.273 ± 0.042	5.344ThrThr: 5.344 ± 0.053	3.256ThrVal: 3.256 ± 0.026	0.39ThrTrp: 0.39 ± 0.006	1.762ThrTyr: 1.762 ± 0.017	0.0ThrXaa: 0.0 ± 0.0
Val	2.836ValAla: 2.836 ± 0.023	1.311ValCys: 1.311 ± 0.034	3.312ValAsp: 3.312 ± 0.02	3.869ValGlu: 3.869 ± 0.029	2.747ValPhe: 2.747 ± 0.022	2.78ValGly: 2.78 ± 0.02	1.106ValHis: 1.106 ± 0.013	3.807ValIle: 3.807 ± 0.025	4.346ValLys: 4.346 ± 0.025	5.089ValLeu: 5.089 ± 0.032	1.335ValMet: 1.335 ± 0.015	3.525ValAsn: 3.525 ± 0.023	2.047ValPro: 2.047 ± 0.019	2.098ValGln: 2.098 ± 0.016	1.993ValArg: 1.993 ± 0.018	4.977ValSer: 4.977 ± 0.03	3.049ValThr: 3.049 ± 0.025	3.746ValVal: 3.746 ± 0.027	0.476ValTrp: 0.476 ± 0.009	1.996ValTyr: 1.996 ± 0.016	0.0ValXaa: 0.0 ± 0.0
Trp	0.314TrpAla: 0.314 ± 0.008	0.143TrpCys: 0.143 ± 0.004	0.444TrpAsp: 0.444 ± 0.009	0.454TrpGlu: 0.454 ± 0.009	0.43TrpPhe: 0.43 ± 0.008	0.353TrpGly: 0.353 ± 0.008	0.132TrpHis: 0.132 ± 0.004	0.652TrpIle: 0.652 ± 0.009	0.858TrpLys: 0.858 ± 0.011	0.677TrpLeu: 0.677 ± 0.009	0.238TrpMet: 0.238 ± 0.006	0.654TrpAsn: 0.654 ± 0.01	0.176TrpPro: 0.176 ± 0.005	0.231TrpGln: 0.231 ± 0.006	0.373TrpArg: 0.373 ± 0.007	0.597TrpSer: 0.597 ± 0.01	0.465TrpThr: 0.465 ± 0.008	0.392TrpVal: 0.392 ± 0.008	0.095TrpTrp: 0.095 ± 0.004	0.326TrpTyr: 0.326 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.563TyrAla: 1.563 ± 0.016	0.839TyrCys: 0.839 ± 0.012	1.95TyrAsp: 1.95 ± 0.017	2.259TyrGlu: 2.259 ± 0.017	2.134TyrPhe: 2.134 ± 0.019	2.081TyrGly: 2.081 ± 0.025	0.91TyrHis: 0.91 ± 0.012	2.321TyrIle: 2.321 ± 0.02	2.119TyrLys: 2.119 ± 0.018	3.734TyrLeu: 3.734 ± 0.023	0.802TyrMet: 0.802 ± 0.01	2.225TyrAsn: 2.225 ± 0.02	1.341TyrPro: 1.341 ± 0.017	1.579TyrGln: 1.579 ± 0.016	1.352TyrArg: 1.352 ± 0.011	3.439TyrSer: 3.439 ± 0.029	1.695TyrThr: 1.695 ± 0.02	1.897TyrVal: 1.897 ± 0.016	0.392TyrTrp: 0.392 ± 0.008	1.812TyrTyr: 1.812 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 15636 proteins (7851348 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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