Amino acid dipepetide frequency for Lactococcus lactis phage P475

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.188AlaAla: 1.188 ± 0.388
0.108AlaCys: 0.108 ± 0.104
2.592AlaAsp: 2.592 ± 0.482
4.967AlaGlu: 4.967 ± 0.878
3.563AlaPhe: 3.563 ± 0.617
4.103AlaGly: 4.103 ± 0.977
0.756AlaHis: 0.756 ± 0.272
4.643AlaIle: 4.643 ± 1.126
6.155AlaLys: 6.155 ± 0.888
5.939AlaLeu: 5.939 ± 0.947
2.16AlaMet: 2.16 ± 0.666
4.319AlaAsn: 4.319 ± 0.803
0.864AlaPro: 0.864 ± 0.322
2.268AlaGln: 2.268 ± 0.573
2.268AlaArg: 2.268 ± 0.478
3.023AlaSer: 3.023 ± 0.762
3.671AlaThr: 3.671 ± 0.731
4.751AlaVal: 4.751 ± 1.233
1.944AlaTrp: 1.944 ± 0.527
2.16AlaTyr: 2.16 ± 0.448
0.0AlaXaa: 0.0 ± 0.0
Cys
0.216CysAla: 0.216 ± 0.145
0.108CysCys: 0.108 ± 0.096
0.216CysAsp: 0.216 ± 0.144
0.216CysGlu: 0.216 ± 0.144
0.0CysPhe: 0.0 ± 0.0
0.756CysGly: 0.756 ± 0.25
0.108CysHis: 0.108 ± 0.102
0.54CysIle: 0.54 ± 0.237
0.54CysLys: 0.54 ± 0.232
0.324CysLeu: 0.324 ± 0.167
0.0CysMet: 0.0 ± 0.0
0.54CysAsn: 0.54 ± 0.236
0.108CysPro: 0.108 ± 0.102
0.216CysGln: 0.216 ± 0.156
0.648CysArg: 0.648 ± 0.243
0.216CysSer: 0.216 ± 0.145
0.324CysThr: 0.324 ± 0.176
0.432CysVal: 0.432 ± 0.188
0.216CysTrp: 0.216 ± 0.172
0.108CysTyr: 0.108 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
2.052AspAla: 2.052 ± 0.532
0.216AspCys: 0.216 ± 0.155
3.239AspAsp: 3.239 ± 0.644
4.427AspGlu: 4.427 ± 0.822
3.455AspPhe: 3.455 ± 0.674
3.995AspGly: 3.995 ± 0.682
0.648AspHis: 0.648 ± 0.269
4.643AspIle: 4.643 ± 0.718
5.939AspLys: 5.939 ± 0.748
5.507AspLeu: 5.507 ± 0.791
1.296AspMet: 1.296 ± 0.372
3.887AspAsn: 3.887 ± 0.774
1.404AspPro: 1.404 ± 0.415
0.54AspGln: 0.54 ± 0.375
2.052AspArg: 2.052 ± 0.463
3.347AspSer: 3.347 ± 0.673
4.427AspThr: 4.427 ± 0.722
3.131AspVal: 3.131 ± 0.786
0.864AspTrp: 0.864 ± 0.323
3.347AspTyr: 3.347 ± 0.546
0.0AspXaa: 0.0 ± 0.0
Glu
3.779GluAla: 3.779 ± 0.593
0.216GluCys: 0.216 ± 0.17
3.023GluAsp: 3.023 ± 0.516
5.831GluGlu: 5.831 ± 1.159
3.455GluPhe: 3.455 ± 0.532
1.944GluGly: 1.944 ± 0.362
1.404GluHis: 1.404 ± 0.414
6.047GluIle: 6.047 ± 0.916
7.451GluLys: 7.451 ± 1.21
9.07GluLeu: 9.07 ± 1.475
2.376GluMet: 2.376 ± 0.592
5.615GluAsn: 5.615 ± 0.792
1.512GluPro: 1.512 ± 0.435
4.103GluGln: 4.103 ± 0.845
3.023GluArg: 3.023 ± 0.565
3.671GluSer: 3.671 ± 0.696
4.859GluThr: 4.859 ± 0.764
5.075GluVal: 5.075 ± 0.854
0.972GluTrp: 0.972 ± 0.271
3.239GluTyr: 3.239 ± 0.725
0.0GluXaa: 0.0 ± 0.0
Phe
3.455PheAla: 3.455 ± 0.775
0.324PheCys: 0.324 ± 0.207
3.131PheAsp: 3.131 ± 0.622
2.376PheGlu: 2.376 ± 0.484
1.728PhePhe: 1.728 ± 0.724
1.944PheGly: 1.944 ± 0.476
0.432PheHis: 0.432 ± 0.206
3.347PheIle: 3.347 ± 0.667
4.643PheLys: 4.643 ± 0.696
2.16PheLeu: 2.16 ± 0.482
1.08PheMet: 1.08 ± 0.324
3.131PheAsn: 3.131 ± 0.989
0.648PhePro: 0.648 ± 0.227
1.08PheGln: 1.08 ± 0.337
1.188PheArg: 1.188 ± 0.373
4.319PheSer: 4.319 ± 1.015
2.592PheThr: 2.592 ± 0.537
3.131PheVal: 3.131 ± 0.432
0.216PheTrp: 0.216 ± 0.165
1.836PheTyr: 1.836 ± 0.464
0.0PheXaa: 0.0 ± 0.0
Gly
3.779GlyAla: 3.779 ± 1.15
0.216GlyCys: 0.216 ± 0.141
3.455GlyAsp: 3.455 ± 0.558
4.535GlyGlu: 4.535 ± 0.616
2.376GlyPhe: 2.376 ± 0.595
4.643GlyGly: 4.643 ± 0.809
0.756GlyHis: 0.756 ± 0.34
4.211GlyIle: 4.211 ± 1.536
7.343GlyLys: 7.343 ± 0.721
5.399GlyLeu: 5.399 ± 0.89
0.648GlyMet: 0.648 ± 0.313
3.563GlyAsn: 3.563 ± 0.685
0.216GlyPro: 0.216 ± 0.158
1.944GlyGln: 1.944 ± 0.463
2.052GlyArg: 2.052 ± 0.368
4.427GlySer: 4.427 ± 0.796
3.131GlyThr: 3.131 ± 0.76
5.723GlyVal: 5.723 ± 1.457
1.188GlyTrp: 1.188 ± 0.33
3.347GlyTyr: 3.347 ± 0.604
0.0GlyXaa: 0.0 ± 0.0
His
0.864HisAla: 0.864 ± 0.254
0.216HisCys: 0.216 ± 0.133
1.188HisAsp: 1.188 ± 0.376
0.648HisGlu: 0.648 ± 0.262
0.432HisPhe: 0.432 ± 0.22
1.512HisGly: 1.512 ± 0.416
0.108HisHis: 0.108 ± 0.121
1.08HisIle: 1.08 ± 0.339
0.864HisLys: 0.864 ± 0.358
0.756HisLeu: 0.756 ± 0.34
0.0HisMet: 0.0 ± 0.0
1.62HisAsn: 1.62 ± 0.457
0.0HisPro: 0.0 ± 0.0
0.54HisGln: 0.54 ± 0.241
0.864HisArg: 0.864 ± 0.331
0.0HisSer: 0.0 ± 0.0
0.648HisThr: 0.648 ± 0.266
0.648HisVal: 0.648 ± 0.262
0.324HisTrp: 0.324 ± 0.276
0.648HisTyr: 0.648 ± 0.298
0.0HisXaa: 0.0 ± 0.0
Ile
4.427IleAla: 4.427 ± 0.82
0.108IleCys: 0.108 ± 0.117
4.859IleAsp: 4.859 ± 0.62
6.695IleGlu: 6.695 ± 1.079
2.807IlePhe: 2.807 ± 0.569
3.455IleGly: 3.455 ± 0.965
0.756IleHis: 0.756 ± 0.266
4.751IleIle: 4.751 ± 0.68
6.587IleLys: 6.587 ± 0.702
5.183IleLeu: 5.183 ± 0.972
1.62IleMet: 1.62 ± 0.383
5.075IleAsn: 5.075 ± 0.681
1.512IlePro: 1.512 ± 0.336
2.484IleGln: 2.484 ± 0.423
1.512IleArg: 1.512 ± 0.442
3.347IleSer: 3.347 ± 0.605
4.751IleThr: 4.751 ± 0.671
4.643IleVal: 4.643 ± 0.708
1.296IleTrp: 1.296 ± 0.365
2.592IleTyr: 2.592 ± 0.506
0.0IleXaa: 0.0 ± 0.0
Lys
6.803LysAla: 6.803 ± 0.934
0.648LysCys: 0.648 ± 0.283
5.291LysAsp: 5.291 ± 0.681
8.854LysGlu: 8.854 ± 1.33
2.807LysPhe: 2.807 ± 0.531
6.803LysGly: 6.803 ± 0.917
1.188LysHis: 1.188 ± 0.432
5.507LysIle: 5.507 ± 0.761
8.854LysLys: 8.854 ± 1.357
7.99LysLeu: 7.99 ± 0.959
3.347LysMet: 3.347 ± 0.465
5.291LysAsn: 5.291 ± 0.723
1.836LysPro: 1.836 ± 0.473
4.427LysGln: 4.427 ± 0.775
3.671LysArg: 3.671 ± 0.733
5.291LysSer: 5.291 ± 0.859
5.507LysThr: 5.507 ± 0.69
6.371LysVal: 6.371 ± 0.703
1.512LysTrp: 1.512 ± 0.367
3.779LysTyr: 3.779 ± 0.738
0.0LysXaa: 0.0 ± 0.0
Leu
5.507LeuAla: 5.507 ± 0.733
0.324LeuCys: 0.324 ± 0.19
4.643LeuAsp: 4.643 ± 0.601
5.939LeuGlu: 5.939 ± 0.909
3.671LeuPhe: 3.671 ± 0.617
4.427LeuGly: 4.427 ± 0.793
1.296LeuHis: 1.296 ± 0.404
6.155LeuIle: 6.155 ± 1.048
8.422LeuLys: 8.422 ± 0.8
6.479LeuLeu: 6.479 ± 1.397
1.728LeuMet: 1.728 ± 0.421
5.075LeuAsn: 5.075 ± 0.868
2.699LeuPro: 2.699 ± 0.595
3.023LeuGln: 3.023 ± 0.48
3.023LeuArg: 3.023 ± 0.518
5.291LeuSer: 5.291 ± 0.882
5.183LeuThr: 5.183 ± 0.659
5.615LeuVal: 5.615 ± 0.695
1.728LeuTrp: 1.728 ± 0.383
3.995LeuTyr: 3.995 ± 0.728
0.0LeuXaa: 0.0 ± 0.0
Met
2.699MetAla: 2.699 ± 0.507
0.0MetCys: 0.0 ± 0.0
1.404MetAsp: 1.404 ± 0.447
1.62MetGlu: 1.62 ± 0.548
0.54MetPhe: 0.54 ± 0.24
0.972MetGly: 0.972 ± 0.326
0.324MetHis: 0.324 ± 0.199
2.376MetIle: 2.376 ± 0.519
3.239MetLys: 3.239 ± 0.643
1.512MetLeu: 1.512 ± 0.426
0.216MetMet: 0.216 ± 0.157
1.728MetAsn: 1.728 ± 0.425
0.54MetPro: 0.54 ± 0.306
1.62MetGln: 1.62 ± 0.393
0.432MetArg: 0.432 ± 0.192
1.188MetSer: 1.188 ± 0.298
1.836MetThr: 1.836 ± 0.571
1.188MetVal: 1.188 ± 0.275
0.216MetTrp: 0.216 ± 0.146
1.296MetTyr: 1.296 ± 0.401
0.0MetXaa: 0.0 ± 0.0
Asn
5.183AsnAla: 5.183 ± 1.163
0.324AsnCys: 0.324 ± 0.16
3.563AsnAsp: 3.563 ± 0.666
5.075AsnGlu: 5.075 ± 0.721
1.944AsnPhe: 1.944 ± 0.593
6.587AsnGly: 6.587 ± 0.885
0.972AsnHis: 0.972 ± 0.278
3.455AsnIle: 3.455 ± 0.671
6.155AsnLys: 6.155 ± 1.036
6.155AsnLeu: 6.155 ± 0.732
1.512AsnMet: 1.512 ± 0.443
3.563AsnAsn: 3.563 ± 0.707
2.16AsnPro: 2.16 ± 0.424
2.16AsnGln: 2.16 ± 0.503
2.052AsnArg: 2.052 ± 0.379
4.643AsnSer: 4.643 ± 0.537
4.535AsnThr: 4.535 ± 0.824
3.131AsnVal: 3.131 ± 0.66
0.972AsnTrp: 0.972 ± 0.364
2.592AsnTyr: 2.592 ± 0.584
0.0AsnXaa: 0.0 ± 0.0
Pro
1.188ProAla: 1.188 ± 0.501
0.216ProCys: 0.216 ± 0.137
1.62ProAsp: 1.62 ± 0.594
1.728ProGlu: 1.728 ± 0.402
0.972ProPhe: 0.972 ± 0.27
0.216ProGly: 0.216 ± 0.15
0.108ProHis: 0.108 ± 0.117
1.62ProIle: 1.62 ± 0.536
2.376ProLys: 2.376 ± 0.552
2.052ProLeu: 2.052 ± 0.443
0.54ProMet: 0.54 ± 0.215
2.052ProAsn: 2.052 ± 0.671
0.756ProPro: 0.756 ± 0.29
0.648ProGln: 0.648 ± 0.275
0.54ProArg: 0.54 ± 0.273
1.404ProSer: 1.404 ± 0.505
2.16ProThr: 2.16 ± 0.536
1.404ProVal: 1.404 ± 0.473
0.216ProTrp: 0.216 ± 0.241
0.432ProTyr: 0.432 ± 0.207
0.0ProXaa: 0.0 ± 0.0
Gln
3.455GlnAla: 3.455 ± 0.756
0.324GlnCys: 0.324 ± 0.157
2.592GlnAsp: 2.592 ± 0.567
2.807GlnGlu: 2.807 ± 0.625
1.512GlnPhe: 1.512 ± 0.389
2.484GlnGly: 2.484 ± 0.441
0.324GlnHis: 0.324 ± 0.179
1.188GlnIle: 1.188 ± 0.284
2.592GlnLys: 2.592 ± 0.583
3.023GlnLeu: 3.023 ± 0.654
1.296GlnMet: 1.296 ± 0.317
2.484GlnAsn: 2.484 ± 0.42
1.404GlnPro: 1.404 ± 0.409
1.512GlnGln: 1.512 ± 0.41
1.404GlnArg: 1.404 ± 0.377
2.376GlnSer: 2.376 ± 0.394
1.944GlnThr: 1.944 ± 0.423
2.16GlnVal: 2.16 ± 0.461
0.648GlnTrp: 0.648 ± 0.22
0.972GlnTyr: 0.972 ± 0.288
0.0GlnXaa: 0.0 ± 0.0
Arg
1.728ArgAla: 1.728 ± 0.583
0.324ArgCys: 0.324 ± 0.165
1.944ArgAsp: 1.944 ± 0.473
2.268ArgGlu: 2.268 ± 0.465
0.972ArgPhe: 0.972 ± 0.315
1.836ArgGly: 1.836 ± 0.405
0.972ArgHis: 0.972 ± 0.334
3.023ArgIle: 3.023 ± 0.591
4.427ArgLys: 4.427 ± 0.894
3.455ArgLeu: 3.455 ± 0.612
0.54ArgMet: 0.54 ± 0.247
2.16ArgAsn: 2.16 ± 0.522
0.648ArgPro: 0.648 ± 0.25
1.62ArgGln: 1.62 ± 0.385
1.728ArgArg: 1.728 ± 0.42
1.836ArgSer: 1.836 ± 0.559
1.512ArgThr: 1.512 ± 0.367
1.836ArgVal: 1.836 ± 0.385
0.108ArgTrp: 0.108 ± 0.082
2.592ArgTyr: 2.592 ± 0.533
0.0ArgXaa: 0.0 ± 0.0
Ser
5.183SerAla: 5.183 ± 1.559
0.648SerCys: 0.648 ± 0.287
4.427SerAsp: 4.427 ± 0.961
3.239SerGlu: 3.239 ± 0.492
3.239SerPhe: 3.239 ± 0.556
4.859SerGly: 4.859 ± 1.421
0.648SerHis: 0.648 ± 0.263
3.779SerIle: 3.779 ± 0.632
5.831SerLys: 5.831 ± 0.745
4.859SerLeu: 4.859 ± 0.695
1.944SerMet: 1.944 ± 0.398
4.427SerAsn: 4.427 ± 0.69
1.08SerPro: 1.08 ± 0.36
1.728SerGln: 1.728 ± 0.501
2.807SerArg: 2.807 ± 0.426
4.319SerSer: 4.319 ± 0.793
2.807SerThr: 2.807 ± 0.608
3.563SerVal: 3.563 ± 0.575
0.864SerTrp: 0.864 ± 0.261
1.62SerTyr: 1.62 ± 0.389
0.0SerXaa: 0.0 ± 0.0
Thr
4.319ThrAla: 4.319 ± 0.569
0.432ThrCys: 0.432 ± 0.164
3.779ThrAsp: 3.779 ± 0.807
5.939ThrGlu: 5.939 ± 0.819
2.807ThrPhe: 2.807 ± 0.567
4.103ThrGly: 4.103 ± 0.521
0.216ThrHis: 0.216 ± 0.148
3.779ThrIle: 3.779 ± 0.679
4.319ThrLys: 4.319 ± 0.59
4.967ThrLeu: 4.967 ± 0.727
1.296ThrMet: 1.296 ± 0.286
4.427ThrAsn: 4.427 ± 0.83
1.728ThrPro: 1.728 ± 0.367
2.699ThrGln: 2.699 ± 0.622
1.836ThrArg: 1.836 ± 0.419
4.103ThrSer: 4.103 ± 0.506
3.347ThrThr: 3.347 ± 0.533
3.671ThrVal: 3.671 ± 0.694
0.864ThrTrp: 0.864 ± 0.227
2.484ThrTyr: 2.484 ± 0.664
0.0ThrXaa: 0.0 ± 0.0
Val
3.887ValAla: 3.887 ± 0.631
0.324ValCys: 0.324 ± 0.187
4.211ValAsp: 4.211 ± 0.698
4.751ValGlu: 4.751 ± 0.565
3.347ValPhe: 3.347 ± 0.738
4.211ValGly: 4.211 ± 0.76
0.972ValHis: 0.972 ± 0.298
4.211ValIle: 4.211 ± 0.515
5.291ValLys: 5.291 ± 0.758
3.239ValLeu: 3.239 ± 0.551
1.944ValMet: 1.944 ± 0.44
2.807ValAsn: 2.807 ± 0.578
1.728ValPro: 1.728 ± 0.452
1.836ValGln: 1.836 ± 0.532
2.807ValArg: 2.807 ± 0.605
5.939ValSer: 5.939 ± 1.231
4.427ValThr: 4.427 ± 0.795
3.347ValVal: 3.347 ± 0.72
1.08ValTrp: 1.08 ± 0.38
2.699ValTyr: 2.699 ± 0.544
0.0ValXaa: 0.0 ± 0.0
Trp
0.432TrpAla: 0.432 ± 0.203
0.216TrpCys: 0.216 ± 0.135
0.756TrpAsp: 0.756 ± 0.457
0.864TrpGlu: 0.864 ± 0.288
0.972TrpPhe: 0.972 ± 0.309
1.08TrpGly: 1.08 ± 0.354
0.216TrpHis: 0.216 ± 0.143
0.54TrpIle: 0.54 ± 0.271
1.404TrpLys: 1.404 ± 0.417
1.836TrpLeu: 1.836 ± 0.648
0.54TrpMet: 0.54 ± 0.219
1.512TrpAsn: 1.512 ± 0.412
0.108TrpPro: 0.108 ± 0.121
0.864TrpGln: 0.864 ± 0.312
0.324TrpArg: 0.324 ± 0.239
1.512TrpSer: 1.512 ± 0.342
0.756TrpThr: 0.756 ± 0.315
0.54TrpVal: 0.54 ± 0.271
0.108TrpTrp: 0.108 ± 0.095
0.972TrpTyr: 0.972 ± 0.263
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.296TyrAla: 1.296 ± 0.403
0.54TyrCys: 0.54 ± 0.335
2.484TyrAsp: 2.484 ± 0.607
3.995TyrGlu: 3.995 ± 0.626
2.16TyrPhe: 2.16 ± 0.498
3.023TyrGly: 3.023 ± 0.631
0.756TyrHis: 0.756 ± 0.24
3.563TyrIle: 3.563 ± 0.748
3.347TyrLys: 3.347 ± 0.808
4.319TyrLeu: 4.319 ± 0.87
0.756TyrMet: 0.756 ± 0.288
3.455TyrAsn: 3.455 ± 0.537
1.296TyrPro: 1.296 ± 0.45
1.188TyrGln: 1.188 ± 0.398
1.296TyrArg: 1.296 ± 0.38
1.62TyrSer: 1.62 ± 0.453
2.699TyrThr: 2.699 ± 0.586
2.699TyrVal: 2.699 ± 0.562
0.216TyrTrp: 0.216 ± 0.182
2.268TyrTyr: 2.268 ± 0.597
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (9262 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski