Amino acid dipepetide frequency for Micromonospora pisi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.615AlaAla: 20.615 ± 0.153
0.87AlaCys: 0.87 ± 0.024
8.266AlaAsp: 8.266 ± 0.064
8.006AlaGlu: 8.006 ± 0.078
3.24AlaPhe: 3.24 ± 0.039
13.62AlaGly: 13.62 ± 0.091
2.372AlaHis: 2.372 ± 0.031
4.252AlaIle: 4.252 ± 0.042
2.229AlaLys: 2.229 ± 0.043
13.758AlaLeu: 13.758 ± 0.113
2.318AlaMet: 2.318 ± 0.025
2.507AlaAsn: 2.507 ± 0.038
7.031AlaPro: 7.031 ± 0.064
3.77AlaGln: 3.77 ± 0.037
9.797AlaArg: 9.797 ± 0.079
6.038AlaSer: 6.038 ± 0.056
8.306AlaThr: 8.306 ± 0.072
11.945AlaVal: 11.945 ± 0.085
1.853AlaTrp: 1.853 ± 0.027
2.725AlaTyr: 2.725 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
0.926CysAla: 0.926 ± 0.02
0.089CysCys: 0.089 ± 0.007
0.458CysAsp: 0.458 ± 0.015
0.315CysGlu: 0.315 ± 0.012
0.196CysPhe: 0.196 ± 0.009
0.835CysGly: 0.835 ± 0.02
0.177CysHis: 0.177 ± 0.009
0.131CysIle: 0.131 ± 0.007
0.081CysLys: 0.081 ± 0.006
0.671CysLeu: 0.671 ± 0.016
0.089CysMet: 0.089 ± 0.006
0.15CysAsn: 0.15 ± 0.008
0.448CysPro: 0.448 ± 0.014
0.189CysGln: 0.189 ± 0.009
0.567CysArg: 0.567 ± 0.016
0.354CysSer: 0.354 ± 0.011
0.433CysThr: 0.433 ± 0.015
0.608CysVal: 0.608 ± 0.015
0.124CysTrp: 0.124 ± 0.006
0.152CysTyr: 0.152 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
6.985AspAla: 6.985 ± 0.066
0.419AspCys: 0.419 ± 0.014
3.608AspAsp: 3.608 ± 0.039
3.743AspGlu: 3.743 ± 0.046
1.506AspPhe: 1.506 ± 0.027
6.296AspGly: 6.296 ± 0.055
1.323AspHis: 1.323 ± 0.021
1.722AspIle: 1.722 ± 0.027
0.862AspLys: 0.862 ± 0.024
6.765AspLeu: 6.765 ± 0.058
0.698AspMet: 0.698 ± 0.017
1.134AspAsn: 1.134 ± 0.023
4.977AspPro: 4.977 ± 0.051
2.04AspGln: 2.04 ± 0.027
5.325AspArg: 5.325 ± 0.054
2.501AspSer: 2.501 ± 0.036
2.877AspThr: 2.877 ± 0.039
4.636AspVal: 4.636 ± 0.053
1.02AspTrp: 1.02 ± 0.022
1.215AspTyr: 1.215 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
6.098GluAla: 6.098 ± 0.064
0.326GluCys: 0.326 ± 0.012
2.018GluAsp: 2.018 ± 0.034
2.52GluGlu: 2.52 ± 0.034
1.489GluPhe: 1.489 ± 0.025
3.117GluGly: 3.117 ± 0.039
1.348GluHis: 1.348 ± 0.026
2.295GluIle: 2.295 ± 0.031
0.916GluLys: 0.916 ± 0.026
6.505GluLeu: 6.505 ± 0.063
0.839GluMet: 0.839 ± 0.018
0.937GluAsn: 0.937 ± 0.021
3.475GluPro: 3.475 ± 0.046
2.318GluGln: 2.318 ± 0.033
4.88GluArg: 4.88 ± 0.053
2.483GluSer: 2.483 ± 0.035
2.695GluThr: 2.695 ± 0.037
4.584GluVal: 4.584 ± 0.054
0.757GluTrp: 0.757 ± 0.017
1.094GluTyr: 1.094 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.648PheAla: 3.648 ± 0.044
0.229PheCys: 0.229 ± 0.01
2.077PheAsp: 2.077 ± 0.032
1.273PheGlu: 1.273 ± 0.022
0.852PhePhe: 0.852 ± 0.021
3.096PheGly: 3.096 ± 0.039
0.565PheHis: 0.565 ± 0.017
0.741PheIle: 0.741 ± 0.019
0.401PheLys: 0.401 ± 0.014
2.44PheLeu: 2.44 ± 0.039
0.345PheMet: 0.345 ± 0.012
0.649PheAsn: 0.649 ± 0.019
1.358PhePro: 1.358 ± 0.025
0.695PheGln: 0.695 ± 0.018
1.804PheArg: 1.804 ± 0.029
1.386PheSer: 1.386 ± 0.028
1.954PheThr: 1.954 ± 0.034
2.463PheVal: 2.463 ± 0.033
0.451PheTrp: 0.451 ± 0.014
0.614PheTyr: 0.614 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
10.916GlyAla: 10.916 ± 0.085
0.797GlyCys: 0.797 ± 0.021
5.158GlyAsp: 5.158 ± 0.041
4.507GlyGlu: 4.507 ± 0.043
2.799GlyPhe: 2.799 ± 0.04
9.008GlyGly: 9.008 ± 0.098
2.045GlyHis: 2.045 ± 0.033
3.235GlyIle: 3.235 ± 0.042
1.897GlyLys: 1.897 ± 0.036
9.504GlyLeu: 9.504 ± 0.083
1.883GlyMet: 1.883 ± 0.027
2.133GlyAsn: 2.133 ± 0.042
5.433GlyPro: 5.433 ± 0.055
3.042GlyGln: 3.042 ± 0.04
7.787GlyArg: 7.787 ± 0.061
5.305GlySer: 5.305 ± 0.056
6.359GlyThr: 6.359 ± 0.062
7.939GlyVal: 7.939 ± 0.067
1.963GlyTrp: 1.963 ± 0.026
2.457GlyTyr: 2.457 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.279HisAla: 2.279 ± 0.033
0.168HisCys: 0.168 ± 0.008
1.231HisAsp: 1.231 ± 0.025
1.03HisGlu: 1.03 ± 0.02
0.56HisPhe: 0.56 ± 0.014
2.053HisGly: 2.053 ± 0.03
0.607HisHis: 0.607 ± 0.018
0.578HisIle: 0.578 ± 0.017
0.254HisLys: 0.254 ± 0.011
2.441HisLeu: 2.441 ± 0.036
0.262HisMet: 0.262 ± 0.01
0.403HisAsn: 0.403 ± 0.013
1.725HisPro: 1.725 ± 0.028
0.709HisGln: 0.709 ± 0.016
2.021HisArg: 2.021 ± 0.033
0.948HisSer: 0.948 ± 0.019
1.127HisThr: 1.127 ± 0.023
1.533HisVal: 1.533 ± 0.026
0.371HisTrp: 0.371 ± 0.013
0.429HisTyr: 0.429 ± 0.012
0.0HisXaa: 0.0 ± 0.0
Ile
4.926IleAla: 4.926 ± 0.049
0.29IleCys: 0.29 ± 0.01
2.432IleAsp: 2.432 ± 0.033
2.06IleGlu: 2.06 ± 0.032
0.931IlePhe: 0.931 ± 0.024
3.814IleGly: 3.814 ± 0.046
0.583IleHis: 0.583 ± 0.016
1.026IleIle: 1.026 ± 0.024
0.646IleLys: 0.646 ± 0.019
2.572IleLeu: 2.572 ± 0.029
0.464IleMet: 0.464 ± 0.012
0.863IleAsn: 0.863 ± 0.022
1.787IlePro: 1.787 ± 0.03
0.79IleGln: 0.79 ± 0.017
2.606IleArg: 2.606 ± 0.032
1.904IleSer: 1.904 ± 0.03
2.283IleThr: 2.283 ± 0.034
2.899IleVal: 2.899 ± 0.037
0.48IleTrp: 0.48 ± 0.016
0.663IleTyr: 0.663 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
1.988LysAla: 1.988 ± 0.035
0.097LysCys: 0.097 ± 0.007
0.776LysAsp: 0.776 ± 0.025
0.726LysGlu: 0.726 ± 0.018
0.388LysPhe: 0.388 ± 0.014
1.189LysGly: 1.189 ± 0.027
0.322LysHis: 0.322 ± 0.013
0.781LysIle: 0.781 ± 0.021
0.435LysLys: 0.435 ± 0.019
1.733LysLeu: 1.733 ± 0.032
0.291LysMet: 0.291 ± 0.01
0.35LysAsn: 0.35 ± 0.013
1.153LysPro: 1.153 ± 0.026
0.581LysGln: 0.581 ± 0.018
1.151LysArg: 1.151 ± 0.028
0.908LysSer: 0.908 ± 0.022
1.039LysThr: 1.039 ± 0.025
1.552LysVal: 1.552 ± 0.031
0.233LysTrp: 0.233 ± 0.01
0.354LysTyr: 0.354 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
16.026LeuAla: 16.026 ± 0.133
0.692LeuCys: 0.692 ± 0.016
6.834LeuAsp: 6.834 ± 0.062
3.864LeuGlu: 3.864 ± 0.046
2.684LeuPhe: 2.684 ± 0.039
9.485LeuGly: 9.485 ± 0.071
2.189LeuHis: 2.189 ± 0.03
3.581LeuIle: 3.581 ± 0.044
1.334LeuLys: 1.334 ± 0.028
11.625LeuLeu: 11.625 ± 0.111
1.402LeuMet: 1.402 ± 0.024
1.813LeuAsn: 1.813 ± 0.028
6.687LeuPro: 6.687 ± 0.068
1.989LeuGln: 1.989 ± 0.034
9.029LeuArg: 9.029 ± 0.067
5.088LeuSer: 5.088 ± 0.047
7.096LeuThr: 7.096 ± 0.063
9.964LeuVal: 9.964 ± 0.079
1.276LeuTrp: 1.276 ± 0.025
1.756LeuTyr: 1.756 ± 0.029
0.0LeuXaa: 0.0 ± 0.0
Met
2.026MetAla: 2.026 ± 0.034
0.11MetCys: 0.11 ± 0.007
0.737MetAsp: 0.737 ± 0.017
0.585MetGlu: 0.585 ± 0.015
0.456MetPhe: 0.456 ± 0.014
1.074MetGly: 1.074 ± 0.021
0.317MetHis: 0.317 ± 0.011
0.721MetIle: 0.721 ± 0.017
0.296MetLys: 0.296 ± 0.011
1.727MetLeu: 1.727 ± 0.026
0.267MetMet: 0.267 ± 0.011
0.393MetAsn: 0.393 ± 0.012
1.071MetPro: 1.071 ± 0.025
0.403MetGln: 0.403 ± 0.014
1.34MetArg: 1.34 ± 0.026
1.246MetSer: 1.246 ± 0.023
1.512MetThr: 1.512 ± 0.023
1.37MetVal: 1.37 ± 0.025
0.201MetTrp: 0.201 ± 0.009
0.284MetTyr: 0.284 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.354AsnAla: 2.354 ± 0.031
0.182AsnCys: 0.182 ± 0.009
1.075AsnAsp: 1.075 ± 0.021
0.845AsnGlu: 0.845 ± 0.018
0.565AsnPhe: 0.565 ± 0.019
2.245AsnGly: 2.245 ± 0.04
0.414AsnHis: 0.414 ± 0.013
0.68AsnIle: 0.68 ± 0.018
0.355AsnLys: 0.355 ± 0.013
2.085AsnLeu: 2.085 ± 0.032
0.309AsnMet: 0.309 ± 0.012
0.576AsnAsn: 0.576 ± 0.019
1.669AsnPro: 1.669 ± 0.03
0.701AsnGln: 0.701 ± 0.018
1.602AsnArg: 1.602 ± 0.028
1.075AsnSer: 1.075 ± 0.025
1.27AsnThr: 1.27 ± 0.034
1.552AsnVal: 1.552 ± 0.028
0.396AsnTrp: 0.396 ± 0.015
0.505AsnTyr: 0.505 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
9.347ProAla: 9.347 ± 0.082
0.294ProCys: 0.294 ± 0.011
4.673ProAsp: 4.673 ± 0.046
4.059ProGlu: 4.059 ± 0.051
1.427ProPhe: 1.427 ± 0.024
6.725ProGly: 6.725 ± 0.059
1.14ProHis: 1.14 ± 0.022
1.778ProIle: 1.778 ± 0.03
0.958ProLys: 0.958 ± 0.024
5.291ProLeu: 5.291 ± 0.059
1.023ProMet: 1.023 ± 0.02
1.215ProAsn: 1.215 ± 0.026
4.308ProPro: 4.308 ± 0.065
1.656ProGln: 1.656 ± 0.03
3.824ProArg: 3.824 ± 0.042
3.3ProSer: 3.3 ± 0.045
4.385ProThr: 4.385 ± 0.053
6.082ProVal: 6.082 ± 0.054
0.904ProTrp: 0.904 ± 0.02
1.31ProTyr: 1.31 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.827GlnAla: 3.827 ± 0.048
0.163GlnCys: 0.163 ± 0.009
1.192GlnAsp: 1.192 ± 0.021
1.228GlnGlu: 1.228 ± 0.022
0.791GlnPhe: 0.791 ± 0.018
2.042GlnGly: 2.042 ± 0.03
0.696GlnHis: 0.696 ± 0.016
1.215GlnIle: 1.215 ± 0.022
0.416GlnLys: 0.416 ± 0.015
3.463GlnLeu: 3.463 ± 0.041
0.475GlnMet: 0.475 ± 0.016
0.552GlnAsn: 0.552 ± 0.017
2.128GlnPro: 2.128 ± 0.036
1.257GlnGln: 1.257 ± 0.026
2.83GlnArg: 2.83 ± 0.038
1.324GlnSer: 1.324 ± 0.025
1.585GlnThr: 1.585 ± 0.029
2.931GlnVal: 2.931 ± 0.04
0.573GlnTrp: 0.573 ± 0.017
0.598GlnTyr: 0.598 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
9.572ArgAla: 9.572 ± 0.074
0.557ArgCys: 0.557 ± 0.018
4.552ArgAsp: 4.552 ± 0.046
4.112ArgGlu: 4.112 ± 0.048
2.473ArgPhe: 2.473 ± 0.031
5.591ArgGly: 5.591 ± 0.058
2.03ArgHis: 2.03 ± 0.033
3.246ArgIle: 3.246 ± 0.041
1.309ArgLys: 1.309 ± 0.028
9.272ArgLeu: 9.272 ± 0.082
1.758ArgMet: 1.758 ± 0.028
1.568ArgAsn: 1.568 ± 0.024
5.135ArgPro: 5.135 ± 0.053
2.799ArgGln: 2.799 ± 0.033
8.102ArgArg: 8.102 ± 0.079
4.208ArgSer: 4.208 ± 0.05
5.141ArgThr: 5.141 ± 0.045
6.369ArgVal: 6.369 ± 0.061
1.65ArgTrp: 1.65 ± 0.024
2.064ArgTyr: 2.064 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.738SerAla: 6.738 ± 0.062
0.371SerCys: 0.371 ± 0.014
2.666SerAsp: 2.666 ± 0.035
2.129SerGlu: 2.129 ± 0.033
1.479SerPhe: 1.479 ± 0.028
6.008SerGly: 6.008 ± 0.062
0.941SerHis: 0.941 ± 0.021
1.708SerIle: 1.708 ± 0.03
0.79SerLys: 0.79 ± 0.019
4.522SerLeu: 4.522 ± 0.044
1.064SerMet: 1.064 ± 0.02
1.049SerAsn: 1.049 ± 0.022
3.246SerPro: 3.246 ± 0.041
1.313SerGln: 1.313 ± 0.023
3.814SerArg: 3.814 ± 0.05
2.818SerSer: 2.818 ± 0.046
3.571SerThr: 3.571 ± 0.049
4.28SerVal: 4.28 ± 0.047
0.98SerTrp: 0.98 ± 0.02
1.307SerTyr: 1.307 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
8.636ThrAla: 8.636 ± 0.071
0.405ThrCys: 0.405 ± 0.014
3.849ThrAsp: 3.849 ± 0.044
3.203ThrGlu: 3.203 ± 0.039
1.75ThrPhe: 1.75 ± 0.026
7.195ThrGly: 7.195 ± 0.061
1.081ThrHis: 1.081 ± 0.02
2.231ThrIle: 2.231 ± 0.036
0.985ThrLys: 0.985 ± 0.024
6.066ThrLeu: 6.066 ± 0.061
0.997ThrMet: 0.997 ± 0.02
1.348ThrAsn: 1.348 ± 0.031
4.47ThrPro: 4.47 ± 0.046
1.403ThrGln: 1.403 ± 0.027
4.196ThrArg: 4.196 ± 0.041
3.422ThrSer: 3.422 ± 0.045
4.332ThrThr: 4.332 ± 0.063
6.535ThrVal: 6.535 ± 0.068
0.981ThrTrp: 0.981 ± 0.02
1.46ThrTyr: 1.46 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
12.147ValAla: 12.147 ± 0.08
0.619ValCys: 0.619 ± 0.016
5.788ValAsp: 5.788 ± 0.051
4.827ValGlu: 4.827 ± 0.051
2.311ValPhe: 2.311 ± 0.033
7.521ValGly: 7.521 ± 0.064
1.721ValHis: 1.721 ± 0.025
3.066ValIle: 3.066 ± 0.042
1.339ValLys: 1.339 ± 0.029
9.574ValLeu: 9.574 ± 0.075
1.186ValMet: 1.186 ± 0.024
1.927ValAsn: 1.927 ± 0.034
5.398ValPro: 5.398 ± 0.057
2.088ValGln: 2.088 ± 0.031
7.228ValArg: 7.228 ± 0.062
4.417ValSer: 4.417 ± 0.043
6.311ValThr: 6.311 ± 0.062
8.611ValVal: 8.611 ± 0.081
1.149ValTrp: 1.149 ± 0.024
1.556ValTyr: 1.556 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.688TrpAla: 1.688 ± 0.03
0.146TrpCys: 0.146 ± 0.007
0.783TrpAsp: 0.783 ± 0.018
0.642TrpGlu: 0.642 ± 0.018
0.511TrpPhe: 0.511 ± 0.016
1.032TrpGly: 1.032 ± 0.021
0.417TrpHis: 0.417 ± 0.012
0.589TrpIle: 0.589 ± 0.014
0.259TrpLys: 0.259 ± 0.011
1.973TrpLeu: 1.973 ± 0.032
0.285TrpMet: 0.285 ± 0.011
0.428TrpAsn: 0.428 ± 0.012
0.934TrpPro: 0.934 ± 0.021
0.728TrpGln: 0.728 ± 0.017
1.62TrpArg: 1.62 ± 0.028
1.124TrpSer: 1.124 ± 0.023
1.036TrpThr: 1.036 ± 0.022
1.141TrpVal: 1.141 ± 0.023
0.402TrpTrp: 0.402 ± 0.013
0.391TrpTyr: 0.391 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.775TyrAla: 2.775 ± 0.034
0.172TyrCys: 0.172 ± 0.009
1.484TyrAsp: 1.484 ± 0.036
1.022TyrGlu: 1.022 ± 0.021
0.631TyrPhe: 0.631 ± 0.015
2.22TyrGly: 2.22 ± 0.031
0.442TyrHis: 0.442 ± 0.013
0.442TyrIle: 0.442 ± 0.015
0.287TyrLys: 0.287 ± 0.012
2.429TyrLeu: 2.429 ± 0.034
0.178TyrMet: 0.178 ± 0.01
0.458TyrAsn: 0.458 ± 0.017
1.264TyrPro: 1.264 ± 0.025
0.816TyrGln: 0.816 ± 0.019
1.985TyrArg: 1.985 ± 0.029
0.945TyrSer: 0.945 ± 0.021
1.231TyrThr: 1.231 ± 0.026
1.789TyrVal: 1.789 ± 0.029
0.364TyrTrp: 0.364 ± 0.012
0.467TyrTyr: 0.467 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7409 proteins (2538772 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski