Roseobacter phage CRP-4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; Cobavirinae; Siovirus; unclassified Siovirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A646QW78|A0A646QW78_9CAUD GNAT family N-acetyltransferase OS=Roseobacter phage CRP-4 OX=2559283 GN=CRP4_gp41 PE=4 SV=1
MM1 pKa = 7.72AKK3 pKa = 9.87QPVVNTISSGYY14 pKa = 10.56ASQSQLNEE22 pKa = 3.82NFSNIQTSFNNTLSLDD38 pKa = 3.56GSTPNAMQANIDD50 pKa = 3.94MNNNDD55 pKa = 4.58IINVSGIYY63 pKa = 10.1VGGVNVLNVLDD74 pKa = 4.09NVTVSTAYY82 pKa = 9.85PSGGNDD88 pKa = 2.78GDD90 pKa = 3.0IWFRR94 pKa = 11.84VSSS97 pKa = 3.69

Molecular weight: 10.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A646QW51|A0A646QW51_9CAUD Nucleotide pyrophosphohydrolase domain protein OS=Roseobacter phage CRP-4 OX=2559283 GN=CRP4_gp22 PE=4 SV=1
MM1 pKa = 8.0AKK3 pKa = 10.24GLYY6 pKa = 10.56ANIHH10 pKa = 6.09AKK12 pKa = 9.73RR13 pKa = 11.84KK14 pKa = 9.62RR15 pKa = 11.84IASGSGEE22 pKa = 4.13KK23 pKa = 9.01MRR25 pKa = 11.84KK26 pKa = 9.05VGSKK30 pKa = 10.07GAPTAKK36 pKa = 10.1NFKK39 pKa = 9.29EE40 pKa = 3.84ASKK43 pKa = 9.02TAKK46 pKa = 9.64KK47 pKa = 9.98RR48 pKa = 11.84KK49 pKa = 9.05KK50 pKa = 10.23

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

12865

33

1455

225.7

25.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.426 ± 0.458

0.731 ± 0.168

6.436 ± 0.28

6.91 ± 0.323

4.073 ± 0.158

7.221 ± 0.373

1.508 ± 0.179

5.387 ± 0.254

6.654 ± 0.468

7.501 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.161

4.975 ± 0.224

3.428 ± 0.136

3.723 ± 0.256

4.78 ± 0.243

7.058 ± 0.324

6.902 ± 0.371

6.623 ± 0.204

1.547 ± 0.161

3.389 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski