Amino acid dipepetide frequency for Roseobacter phage CRP-4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.841AlaAla: 6.841 ± 1.049
0.622AlaCys: 0.622 ± 0.335
3.965AlaAsp: 3.965 ± 0.633
5.519AlaGlu: 5.519 ± 0.615
3.42AlaPhe: 3.42 ± 0.515
7.851AlaGly: 7.851 ± 0.956
0.933AlaHis: 0.933 ± 0.227
4.509AlaIle: 4.509 ± 0.511
6.141AlaLys: 6.141 ± 0.831
6.374AlaLeu: 6.374 ± 0.828
1.943AlaMet: 1.943 ± 0.452
3.576AlaAsn: 3.576 ± 0.491
2.41AlaPro: 2.41 ± 0.419
2.332AlaGln: 2.332 ± 0.374
4.275AlaArg: 4.275 ± 0.703
7.385AlaSer: 7.385 ± 0.934
5.908AlaThr: 5.908 ± 0.735
4.897AlaVal: 4.897 ± 0.869
1.477AlaTrp: 1.477 ± 0.396
3.809AlaTyr: 3.809 ± 0.565
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.311CysAsp: 0.311 ± 0.151
0.544CysGlu: 0.544 ± 0.259
0.311CysPhe: 0.311 ± 0.18
0.466CysGly: 0.466 ± 0.211
0.466CysHis: 0.466 ± 0.226
0.466CysIle: 0.466 ± 0.233
0.777CysLys: 0.777 ± 0.264
0.155CysLeu: 0.155 ± 0.111
0.311CysMet: 0.311 ± 0.155
0.311CysAsn: 0.311 ± 0.166
0.389CysPro: 0.389 ± 0.19
0.233CysGln: 0.233 ± 0.184
0.389CysArg: 0.389 ± 0.185
0.544CysSer: 0.544 ± 0.26
0.466CysThr: 0.466 ± 0.2
0.7CysVal: 0.7 ± 0.23
0.233CysTrp: 0.233 ± 0.154
0.233CysTyr: 0.233 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
5.752AspAla: 5.752 ± 0.852
0.855AspCys: 0.855 ± 0.279
3.42AspAsp: 3.42 ± 0.592
3.109AspGlu: 3.109 ± 0.474
3.887AspPhe: 3.887 ± 0.837
4.586AspGly: 4.586 ± 0.725
0.777AspHis: 0.777 ± 0.307
4.198AspIle: 4.198 ± 0.635
3.887AspLys: 3.887 ± 0.744
5.442AspLeu: 5.442 ± 0.61
2.177AspMet: 2.177 ± 0.493
4.042AspAsn: 4.042 ± 0.881
3.032AspPro: 3.032 ± 0.506
1.322AspGln: 1.322 ± 0.324
1.866AspArg: 1.866 ± 0.398
3.965AspSer: 3.965 ± 0.56
3.498AspThr: 3.498 ± 0.487
4.897AspVal: 4.897 ± 0.684
1.088AspTrp: 1.088 ± 0.333
2.565AspTyr: 2.565 ± 0.449
0.0AspXaa: 0.0 ± 0.0
Glu
5.675GluAla: 5.675 ± 0.717
0.466GluCys: 0.466 ± 0.196
4.975GluAsp: 4.975 ± 0.649
4.586GluGlu: 4.586 ± 0.663
2.721GluPhe: 2.721 ± 0.406
5.519GluGly: 5.519 ± 0.772
1.555GluHis: 1.555 ± 0.397
3.731GluIle: 3.731 ± 0.516
3.576GluLys: 3.576 ± 0.627
5.752GluLeu: 5.752 ± 0.604
2.021GluMet: 2.021 ± 0.442
3.576GluAsn: 3.576 ± 0.606
2.021GluPro: 2.021 ± 0.481
2.954GluGln: 2.954 ± 0.659
3.576GluArg: 3.576 ± 0.378
3.654GluSer: 3.654 ± 0.475
3.965GluThr: 3.965 ± 0.479
4.742GluVal: 4.742 ± 0.575
1.632GluTrp: 1.632 ± 0.374
2.41GluTyr: 2.41 ± 0.399
0.0GluXaa: 0.0 ± 0.0
Phe
3.343PheAla: 3.343 ± 0.469
0.233PheCys: 0.233 ± 0.129
3.187PheAsp: 3.187 ± 0.4
2.565PheGlu: 2.565 ± 0.463
1.399PhePhe: 1.399 ± 0.319
2.565PheGly: 2.565 ± 0.523
0.7PheHis: 0.7 ± 0.209
2.565PheIle: 2.565 ± 0.399
3.42PheLys: 3.42 ± 0.623
2.488PheLeu: 2.488 ± 0.311
1.244PheMet: 1.244 ± 0.328
2.41PheAsn: 2.41 ± 0.421
0.855PhePro: 0.855 ± 0.157
1.166PheGln: 1.166 ± 0.285
1.866PheArg: 1.866 ± 0.398
2.876PheSer: 2.876 ± 0.514
3.498PheThr: 3.498 ± 0.529
2.41PheVal: 2.41 ± 0.399
0.233PheTrp: 0.233 ± 0.134
1.71PheTyr: 1.71 ± 0.39
0.0PheXaa: 0.0 ± 0.0
Gly
5.752GlyAla: 5.752 ± 0.867
0.233GlyCys: 0.233 ± 0.134
4.664GlyAsp: 4.664 ± 0.602
4.975GlyGlu: 4.975 ± 0.623
4.897GlyPhe: 4.897 ± 0.909
5.208GlyGly: 5.208 ± 0.945
1.244GlyHis: 1.244 ± 0.397
4.431GlyIle: 4.431 ± 0.496
4.897GlyLys: 4.897 ± 0.662
4.897GlyLeu: 4.897 ± 0.634
1.71GlyMet: 1.71 ± 0.44
3.42GlyAsn: 3.42 ± 0.461
1.632GlyPro: 1.632 ± 0.412
1.943GlyGln: 1.943 ± 0.371
3.965GlyArg: 3.965 ± 0.574
5.131GlySer: 5.131 ± 0.676
4.586GlyThr: 4.586 ± 0.85
5.286GlyVal: 5.286 ± 0.632
1.011GlyTrp: 1.011 ± 0.261
2.332GlyTyr: 2.332 ± 0.323
0.0GlyXaa: 0.0 ± 0.0
His
0.777HisAla: 0.777 ± 0.267
0.233HisCys: 0.233 ± 0.145
0.7HisAsp: 0.7 ± 0.291
1.088HisGlu: 1.088 ± 0.325
0.7HisPhe: 0.7 ± 0.255
0.855HisGly: 0.855 ± 0.301
0.155HisHis: 0.155 ± 0.108
1.088HisIle: 1.088 ± 0.318
1.166HisLys: 1.166 ± 0.32
1.322HisLeu: 1.322 ± 0.351
0.622HisMet: 0.622 ± 0.251
0.466HisAsn: 0.466 ± 0.221
0.777HisPro: 0.777 ± 0.266
0.544HisGln: 0.544 ± 0.236
0.855HisArg: 0.855 ± 0.317
1.244HisSer: 1.244 ± 0.355
1.244HisThr: 1.244 ± 0.314
0.544HisVal: 0.544 ± 0.234
0.233HisTrp: 0.233 ± 0.131
0.466HisTyr: 0.466 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
4.82IleAla: 4.82 ± 0.517
0.544IleCys: 0.544 ± 0.217
4.431IleAsp: 4.431 ± 0.632
4.12IleGlu: 4.12 ± 0.521
1.632IlePhe: 1.632 ± 0.293
2.332IleGly: 2.332 ± 0.499
0.855IleHis: 0.855 ± 0.3
2.41IleIle: 2.41 ± 0.455
4.586IleLys: 4.586 ± 0.594
3.42IleLeu: 3.42 ± 0.649
1.011IleMet: 1.011 ± 0.256
3.109IleAsn: 3.109 ± 0.487
1.866IlePro: 1.866 ± 0.404
2.099IleGln: 2.099 ± 0.436
2.643IleArg: 2.643 ± 0.374
3.576IleSer: 3.576 ± 0.517
3.887IleThr: 3.887 ± 0.651
4.586IleVal: 4.586 ± 0.657
0.777IleTrp: 0.777 ± 0.278
1.166IleTyr: 1.166 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
5.364LysAla: 5.364 ± 0.724
0.233LysCys: 0.233 ± 0.131
4.198LysAsp: 4.198 ± 0.802
5.519LysGlu: 5.519 ± 0.816
1.788LysPhe: 1.788 ± 0.381
5.442LysGly: 5.442 ± 0.965
1.166LysHis: 1.166 ± 0.368
3.42LysIle: 3.42 ± 0.541
4.275LysLys: 4.275 ± 0.813
4.509LysLeu: 4.509 ± 0.586
2.099LysMet: 2.099 ± 0.342
2.177LysAsn: 2.177 ± 0.292
2.799LysPro: 2.799 ± 0.628
2.799LysGln: 2.799 ± 0.585
4.042LysArg: 4.042 ± 0.528
3.809LysSer: 3.809 ± 0.625
5.83LysThr: 5.83 ± 0.708
3.965LysVal: 3.965 ± 0.498
1.166LysTrp: 1.166 ± 0.258
1.943LysTyr: 1.943 ± 0.344
0.0LysXaa: 0.0 ± 0.0
Leu
6.374LeuAla: 6.374 ± 0.661
0.233LeuCys: 0.233 ± 0.138
6.53LeuAsp: 6.53 ± 0.797
6.219LeuGlu: 6.219 ± 0.724
2.876LeuPhe: 2.876 ± 0.409
5.675LeuGly: 5.675 ± 0.741
0.622LeuHis: 0.622 ± 0.228
3.343LeuIle: 3.343 ± 0.502
5.208LeuLys: 5.208 ± 0.608
4.82LeuLeu: 4.82 ± 0.705
1.632LeuMet: 1.632 ± 0.418
4.12LeuAsn: 4.12 ± 0.522
2.254LeuPro: 2.254 ± 0.538
2.643LeuGln: 2.643 ± 0.422
4.042LeuArg: 4.042 ± 0.469
5.597LeuSer: 5.597 ± 0.689
5.519LeuThr: 5.519 ± 0.731
4.975LeuVal: 4.975 ± 0.623
0.622LeuTrp: 0.622 ± 0.213
1.71LeuTyr: 1.71 ± 0.334
0.0LeuXaa: 0.0 ± 0.0
Met
3.187MetAla: 3.187 ± 0.553
0.466MetCys: 0.466 ± 0.191
1.555MetAsp: 1.555 ± 0.327
2.099MetGlu: 2.099 ± 0.431
0.777MetPhe: 0.777 ± 0.269
1.399MetGly: 1.399 ± 0.357
0.544MetHis: 0.544 ± 0.251
1.399MetIle: 1.399 ± 0.35
1.244MetLys: 1.244 ± 0.292
1.788MetLeu: 1.788 ± 0.457
0.7MetMet: 0.7 ± 0.337
0.933MetAsn: 0.933 ± 0.221
1.088MetPro: 1.088 ± 0.383
1.632MetGln: 1.632 ± 0.381
2.021MetArg: 2.021 ± 0.424
2.177MetSer: 2.177 ± 0.384
1.866MetThr: 1.866 ± 0.374
1.166MetVal: 1.166 ± 0.287
0.622MetTrp: 0.622 ± 0.22
0.622MetTyr: 0.622 ± 0.223
0.0MetXaa: 0.0 ± 0.0
Asn
3.576AsnAla: 3.576 ± 0.725
0.544AsnCys: 0.544 ± 0.194
3.654AsnAsp: 3.654 ± 0.577
2.332AsnGlu: 2.332 ± 0.398
1.477AsnPhe: 1.477 ± 0.375
3.809AsnGly: 3.809 ± 0.779
0.7AsnHis: 0.7 ± 0.274
3.265AsnIle: 3.265 ± 0.472
3.032AsnLys: 3.032 ± 0.514
4.742AsnLeu: 4.742 ± 0.526
1.011AsnMet: 1.011 ± 0.294
2.721AsnAsn: 2.721 ± 0.437
3.109AsnPro: 3.109 ± 0.521
1.788AsnGln: 1.788 ± 0.319
2.254AsnArg: 2.254 ± 0.364
3.109AsnSer: 3.109 ± 0.426
3.265AsnThr: 3.265 ± 0.596
2.876AsnVal: 2.876 ± 0.543
0.855AsnTrp: 0.855 ± 0.258
1.632AsnTyr: 1.632 ± 0.314
0.0AsnXaa: 0.0 ± 0.0
Pro
1.788ProAla: 1.788 ± 0.385
0.466ProCys: 0.466 ± 0.24
2.332ProAsp: 2.332 ± 0.363
3.265ProGlu: 3.265 ± 0.669
0.7ProPhe: 0.7 ± 0.185
1.399ProGly: 1.399 ± 0.348
1.088ProHis: 1.088 ± 0.263
1.555ProIle: 1.555 ± 0.362
2.721ProLys: 2.721 ± 0.441
2.41ProLeu: 2.41 ± 0.455
1.011ProMet: 1.011 ± 0.306
1.788ProAsn: 1.788 ± 0.372
0.466ProPro: 0.466 ± 0.161
1.477ProGln: 1.477 ± 0.395
1.166ProArg: 1.166 ± 0.354
3.498ProSer: 3.498 ± 0.427
2.799ProThr: 2.799 ± 0.42
2.488ProVal: 2.488 ± 0.308
0.544ProTrp: 0.544 ± 0.208
1.322ProTyr: 1.322 ± 0.326
0.0ProXaa: 0.0 ± 0.0
Gln
4.586GlnAla: 4.586 ± 0.699
0.155GlnCys: 0.155 ± 0.116
1.477GlnAsp: 1.477 ± 0.29
3.265GlnGlu: 3.265 ± 0.647
1.71GlnPhe: 1.71 ± 0.456
2.488GlnGly: 2.488 ± 0.368
0.155GlnHis: 0.155 ± 0.108
2.565GlnIle: 2.565 ± 0.47
2.021GlnLys: 2.021 ± 0.389
3.343GlnLeu: 3.343 ± 0.544
1.088GlnMet: 1.088 ± 0.339
1.477GlnAsn: 1.477 ± 0.363
1.244GlnPro: 1.244 ± 0.313
2.488GlnGln: 2.488 ± 0.566
1.71GlnArg: 1.71 ± 0.326
1.866GlnSer: 1.866 ± 0.343
2.41GlnThr: 2.41 ± 0.527
1.322GlnVal: 1.322 ± 0.333
0.622GlnTrp: 0.622 ± 0.197
1.244GlnTyr: 1.244 ± 0.408
0.0GlnXaa: 0.0 ± 0.0
Arg
3.576ArgAla: 3.576 ± 0.449
0.233ArgCys: 0.233 ± 0.151
3.809ArgAsp: 3.809 ± 0.615
3.032ArgGlu: 3.032 ± 0.492
1.555ArgPhe: 1.555 ± 0.345
2.799ArgGly: 2.799 ± 0.471
1.088ArgHis: 1.088 ± 0.374
3.265ArgIle: 3.265 ± 0.637
4.12ArgLys: 4.12 ± 0.882
4.198ArgLeu: 4.198 ± 0.757
2.021ArgMet: 2.021 ± 0.342
2.41ArgAsn: 2.41 ± 0.382
1.555ArgPro: 1.555 ± 0.257
1.399ArgGln: 1.399 ± 0.426
2.721ArgArg: 2.721 ± 0.386
2.799ArgSer: 2.799 ± 0.401
2.41ArgThr: 2.41 ± 0.374
2.099ArgVal: 2.099 ± 0.475
0.933ArgTrp: 0.933 ± 0.299
1.788ArgTyr: 1.788 ± 0.478
0.0ArgXaa: 0.0 ± 0.0
Ser
6.219SerAla: 6.219 ± 0.787
0.389SerCys: 0.389 ± 0.203
3.965SerAsp: 3.965 ± 0.639
3.731SerGlu: 3.731 ± 0.512
3.576SerPhe: 3.576 ± 0.457
6.297SerGly: 6.297 ± 0.821
0.777SerHis: 0.777 ± 0.285
3.032SerIle: 3.032 ± 0.412
3.965SerLys: 3.965 ± 0.477
4.82SerLeu: 4.82 ± 0.538
1.71SerMet: 1.71 ± 0.343
4.275SerAsn: 4.275 ± 0.543
2.332SerPro: 2.332 ± 0.462
3.498SerGln: 3.498 ± 0.612
2.565SerArg: 2.565 ± 0.565
5.986SerSer: 5.986 ± 0.916
4.897SerThr: 4.897 ± 0.579
4.742SerVal: 4.742 ± 0.685
0.855SerTrp: 0.855 ± 0.255
2.954SerTyr: 2.954 ± 0.505
0.0SerXaa: 0.0 ± 0.0
Thr
6.452ThrAla: 6.452 ± 1.028
0.311ThrCys: 0.311 ± 0.153
3.809ThrAsp: 3.809 ± 0.695
5.519ThrGlu: 5.519 ± 0.615
2.721ThrPhe: 2.721 ± 0.419
5.519ThrGly: 5.519 ± 1.108
0.933ThrHis: 0.933 ± 0.23
3.187ThrIle: 3.187 ± 0.525
4.042ThrLys: 4.042 ± 0.628
5.908ThrLeu: 5.908 ± 0.667
2.021ThrMet: 2.021 ± 0.326
3.187ThrAsn: 3.187 ± 0.635
2.41ThrPro: 2.41 ± 0.366
2.954ThrGln: 2.954 ± 0.449
2.643ThrArg: 2.643 ± 0.465
4.586ThrSer: 4.586 ± 0.765
4.975ThrThr: 4.975 ± 0.778
4.82ThrVal: 4.82 ± 0.549
0.777ThrTrp: 0.777 ± 0.258
2.254ThrTyr: 2.254 ± 0.462
0.0ThrXaa: 0.0 ± 0.0
Val
6.374ValAla: 6.374 ± 0.801
0.389ValCys: 0.389 ± 0.177
4.664ValAsp: 4.664 ± 0.498
4.509ValGlu: 4.509 ± 0.565
2.643ValPhe: 2.643 ± 0.363
5.131ValGly: 5.131 ± 0.549
0.622ValHis: 0.622 ± 0.268
3.109ValIle: 3.109 ± 0.481
4.353ValLys: 4.353 ± 0.457
4.586ValLeu: 4.586 ± 0.741
1.399ValMet: 1.399 ± 0.327
3.42ValAsn: 3.42 ± 0.682
2.254ValPro: 2.254 ± 0.444
1.71ValGln: 1.71 ± 0.491
2.488ValArg: 2.488 ± 0.449
6.063ValSer: 6.063 ± 0.652
3.965ValThr: 3.965 ± 0.611
5.675ValVal: 5.675 ± 0.63
0.933ValTrp: 0.933 ± 0.243
1.943ValTyr: 1.943 ± 0.46
0.0ValXaa: 0.0 ± 0.0
Trp
1.477TrpAla: 1.477 ± 0.335
0.233TrpCys: 0.233 ± 0.127
0.933TrpAsp: 0.933 ± 0.257
0.933TrpGlu: 0.933 ± 0.287
0.622TrpPhe: 0.622 ± 0.198
0.622TrpGly: 0.622 ± 0.192
0.233TrpHis: 0.233 ± 0.124
0.233TrpIle: 0.233 ± 0.117
0.855TrpLys: 0.855 ± 0.256
1.399TrpLeu: 1.399 ± 0.355
0.544TrpMet: 0.544 ± 0.212
0.544TrpAsn: 0.544 ± 0.195
0.777TrpPro: 0.777 ± 0.354
0.544TrpGln: 0.544 ± 0.188
1.166TrpArg: 1.166 ± 0.315
1.088TrpSer: 1.088 ± 0.292
1.088TrpThr: 1.088 ± 0.31
1.088TrpVal: 1.088 ± 0.276
0.311TrpTrp: 0.311 ± 0.129
0.777TrpTyr: 0.777 ± 0.209
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.332TyrAla: 2.332 ± 0.357
0.466TyrCys: 0.466 ± 0.193
1.788TyrAsp: 1.788 ± 0.348
2.177TyrGlu: 2.177 ± 0.45
1.322TyrPhe: 1.322 ± 0.305
2.254TyrGly: 2.254 ± 0.339
0.466TyrHis: 0.466 ± 0.245
2.099TyrIle: 2.099 ± 0.432
2.254TyrLys: 2.254 ± 0.498
2.643TyrLeu: 2.643 ± 0.536
0.933TyrMet: 0.933 ± 0.279
1.788TyrAsn: 1.788 ± 0.321
1.011TyrPro: 1.011 ± 0.389
1.71TyrGln: 1.71 ± 0.395
1.555TyrArg: 1.555 ± 0.302
1.632TyrSer: 1.632 ± 0.384
2.954TyrThr: 2.954 ± 0.455
2.954TyrVal: 2.954 ± 0.494
0.544TyrTrp: 0.544 ± 0.217
1.011TyrTyr: 1.011 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (12865 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski