Brugia malayi (Filarial nematode worm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Brugia

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10206 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5S6PD84|A0A5S6PD84_BRUMA Uncharacterized protein OS=Brugia malayi OX=6279 PE=4 SV=1
MM1 pKa = 7.14VSSDD5 pKa = 3.78EE6 pKa = 4.89FRR8 pKa = 11.84DD9 pKa = 3.57ADD11 pKa = 3.74DD12 pKa = 4.2VLFSTSNEE20 pKa = 3.63VTYY23 pKa = 10.59IAFDD27 pKa = 3.82GADD30 pKa = 3.43CKK32 pKa = 10.11YY33 pKa = 9.69TPGDD37 pKa = 3.82CFDD40 pKa = 4.45PAVMTRR46 pKa = 11.84IKK48 pKa = 10.42LSCHH52 pKa = 5.29SRR54 pKa = 11.84LFNATSFDD62 pKa = 4.25QIPEE66 pKa = 4.23LLDD69 pKa = 5.09DD70 pKa = 5.68IIIPDD75 pKa = 3.74YY76 pKa = 11.17CSFGDD81 pKa = 4.56SVDD84 pKa = 3.22INIWIGPSEE93 pKa = 4.39NVSSPYY99 pKa = 10.03FDD101 pKa = 4.0PKK103 pKa = 11.44SNIFAKK109 pKa = 10.65

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5S6PD00|A0A5S6PD00_BRUMA BMA-MEMB-1 OS=Brugia malayi OX=6279 PE=4 SV=1
MM1 pKa = 7.71LANCYY6 pKa = 7.8WQLDD10 pKa = 3.67HH11 pKa = 6.99QGRR14 pKa = 11.84SRR16 pKa = 11.84KK17 pKa = 9.71EE18 pKa = 3.63GGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGFGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGFGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGFRR1160 pKa = 11.84LRR1162 pKa = 11.84LRR1164 pKa = 11.84LRR1166 pKa = 11.84LRR1168 pKa = 11.84LRR1170 pKa = 11.84LRR1172 pKa = 11.84LRR1174 pKa = 11.84LRR1176 pKa = 11.84LRR1178 pKa = 11.84LRR1180 pKa = 11.84LRR1182 pKa = 11.84LRR1184 pKa = 11.84LRR1186 pKa = 11.84LRR1188 pKa = 11.84LRR1190 pKa = 11.84LRR1192 pKa = 11.84LRR1194 pKa = 11.84LRR1196 pKa = 11.84LRR1198 pKa = 11.84LRR1200 pKa = 11.84LRR1202 pKa = 11.84LRR1204 pKa = 11.84LRR1206 pKa = 11.84LRR1208 pKa = 11.84LRR1210 pKa = 11.84LRR1212 pKa = 11.84LRR1214 pKa = 11.84LRR1216 pKa = 11.84LRR1218 pKa = 11.84LRR1220 pKa = 11.84LRR1222 pKa = 11.84LRR1224 pKa = 11.84LRR1226 pKa = 11.84LRR1228 pKa = 11.84LRR1230 pKa = 11.84LRR1232 pKa = 11.84LRR1234 pKa = 11.84LRR1236 pKa = 11.84LRR1238 pKa = 11.84LRR1240 pKa = 11.84LRR1242 pKa = 11.84LRR1244 pKa = 11.84LRR1246 pKa = 11.84LRR1248 pKa = 11.84LRR1250 pKa = 11.84LRR1252 pKa = 11.84LSLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGFGLGLGLGLGLGLGLGLGLGLGLGLGLGLAA1400 pKa = 4.62

Molecular weight: 124.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8825

1381

10206

4761086

15

11701

466.5

52.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.109 ± 0.02

2.205 ± 0.024

5.461 ± 0.018

6.685 ± 0.048

4.077 ± 0.021

5.099 ± 0.037

2.426 ± 0.011

6.502 ± 0.026

6.225 ± 0.035

9.235 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.013

5.167 ± 0.021

4.275 ± 0.028

4.259 ± 0.025

5.673 ± 0.023

8.347 ± 0.03

5.639 ± 0.02

5.863 ± 0.021

1.082 ± 0.009

3.143 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski