Lactobacillus brantae DSM 23927

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus; Lacticaseibacillus brantae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1863 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R2BA67|A0A0R2BA67_9LACO M3B subfamily peptidase OS=Lactobacillus brantae DSM 23927 OX=1423727 GN=FC34_GL000783 PE=3 SV=1
MM1 pKa = 7.53SYY3 pKa = 10.56SEE5 pKa = 4.24QMLDD9 pKa = 4.25ALAEE13 pKa = 4.44GNVDD17 pKa = 3.44QANLLFRR24 pKa = 11.84SVLEE28 pKa = 4.5HH29 pKa = 7.77DD30 pKa = 4.96DD31 pKa = 6.15DD32 pKa = 3.88EE33 pKa = 4.84TKK35 pKa = 11.29YY36 pKa = 11.25NLAEE40 pKa = 3.86EE41 pKa = 5.23LYY43 pKa = 10.73ALGFTGQAKK52 pKa = 9.95RR53 pKa = 11.84LYY55 pKa = 8.71QEE57 pKa = 3.94LMGRR61 pKa = 11.84YY62 pKa = 8.68PDD64 pKa = 3.45QDD66 pKa = 4.01DD67 pKa = 3.96IKK69 pKa = 10.39TVLADD74 pKa = 3.36IAVSDD79 pKa = 4.36GDD81 pKa = 3.78PDD83 pKa = 3.56AALNYY88 pKa = 9.87LSKK91 pKa = 10.49IQPDD95 pKa = 3.47SEE97 pKa = 5.32AYY99 pKa = 10.6LEE101 pKa = 4.35SLVTAADD108 pKa = 4.09VYY110 pKa = 9.24QTQGMYY116 pKa = 9.89EE117 pKa = 3.97VSEE120 pKa = 4.22QKK122 pKa = 10.71LLQAQAIAPDD132 pKa = 3.6EE133 pKa = 4.0PVIAFALGEE142 pKa = 4.4LYY144 pKa = 10.38FDD146 pKa = 3.6WGHH149 pKa = 6.0YY150 pKa = 8.96PQAQQAYY157 pKa = 8.91AQLLAAGEE165 pKa = 4.36TEE167 pKa = 4.05LAGVDD172 pKa = 3.6VQARR176 pKa = 11.84LAATLAQLGQYY187 pKa = 9.5EE188 pKa = 4.39DD189 pKa = 5.57AIATYY194 pKa = 9.81EE195 pKa = 3.93ADD197 pKa = 5.5GINQLDD203 pKa = 4.05LNNRR207 pKa = 11.84FQLGGLYY214 pKa = 10.61YY215 pKa = 10.85LVQDD219 pKa = 3.32YY220 pKa = 10.18RR221 pKa = 11.84AAIDD225 pKa = 3.59AFSGVLEE232 pKa = 4.03QDD234 pKa = 2.94QSFASAYY241 pKa = 10.49LPLAQAYY248 pKa = 9.3VADD251 pKa = 4.44NQPDD255 pKa = 3.55MALEE259 pKa = 4.88TIQDD263 pKa = 3.56GVMVDD268 pKa = 3.69DD269 pKa = 4.9TNPDD273 pKa = 3.76LYY275 pKa = 11.53ALGADD280 pKa = 3.96LAIKK284 pKa = 10.62AEE286 pKa = 4.08QNDD289 pKa = 3.64LAEE292 pKa = 4.81NYY294 pKa = 8.99LQKK297 pKa = 11.14ALAIDD302 pKa = 4.09PEE304 pKa = 4.27NLTNALAWSNYY315 pKa = 9.65LYY317 pKa = 10.67LAEE320 pKa = 4.94RR321 pKa = 11.84DD322 pKa = 3.46EE323 pKa = 5.47DD324 pKa = 4.5NIAFLNGLDD333 pKa = 3.87QEE335 pKa = 5.18GEE337 pKa = 4.06VDD339 pKa = 5.01PQINWNLAKK348 pKa = 10.46SEE350 pKa = 3.97ARR352 pKa = 11.84LDD354 pKa = 4.45EE355 pKa = 4.46IDD357 pKa = 3.41QARR360 pKa = 11.84EE361 pKa = 3.7HH362 pKa = 6.56YY363 pKa = 10.75LLAFNRR369 pKa = 11.84LQTQPDD375 pKa = 4.18FLHH378 pKa = 7.3DD379 pKa = 4.69IIDD382 pKa = 4.38FFQMTGAIDD391 pKa = 3.5EE392 pKa = 4.82TKK394 pKa = 10.94AAMQRR399 pKa = 11.84YY400 pKa = 7.95LQLVPDD406 pKa = 4.88DD407 pKa = 5.33LDD409 pKa = 3.37MQEE412 pKa = 4.76RR413 pKa = 11.84YY414 pKa = 10.4DD415 pKa = 4.99RR416 pKa = 11.84LIADD420 pKa = 4.12

Molecular weight: 47.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R2AXI8|A0A0R2AXI8_9LACO 50S ribosomal protein L24 OS=Lactobacillus brantae DSM 23927 OX=1423727 GN=rplX PE=3 SV=1
MM1 pKa = 5.72TTKK4 pKa = 9.85RR5 pKa = 11.84TFQPKK10 pKa = 8.33KK11 pKa = 7.5RR12 pKa = 11.84HH13 pKa = 5.56RR14 pKa = 11.84EE15 pKa = 3.79RR16 pKa = 11.84VHH18 pKa = 7.7GFMKK22 pKa = 10.56RR23 pKa = 11.84MSTKK27 pKa = 10.26NGRR30 pKa = 11.84KK31 pKa = 8.58VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.94GRR41 pKa = 11.84KK42 pKa = 8.67VLSAA46 pKa = 4.05

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1863

0

1863

572218

46

4345

307.1

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.621 ± 0.06

0.214 ± 0.01

5.995 ± 0.058

4.84 ± 0.059

4.204 ± 0.045

7.06 ± 0.051

2.093 ± 0.031

6.443 ± 0.056

4.83 ± 0.05

10.139 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.035

4.318 ± 0.057

4.139 ± 0.035

5.118 ± 0.056

4.124 ± 0.046

5.616 ± 0.056

6.665 ± 0.063

7.52 ± 0.041

1.151 ± 0.025

3.273 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski