Prevotella sp. tf2-5

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; unclassified Prevotella

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2923 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I5J1I8|A0A1I5J1I8_9BACT DNA-binding protein histone-like putative OS=Prevotella sp. tf2-5 OX=1761889 GN=SAMN04487852_104209 PE=4 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.06YY4 pKa = 10.18KK5 pKa = 10.11IEE7 pKa = 4.26CEE9 pKa = 3.78QFLGFSHH16 pKa = 6.57SGSVTTSGEE25 pKa = 4.22STIEE29 pKa = 3.88LSDD32 pKa = 3.95EE33 pKa = 4.11EE34 pKa = 4.85VATLVQLIRR43 pKa = 11.84EE44 pKa = 4.24KK45 pKa = 10.63GTTDD49 pKa = 2.97VGEE52 pKa = 4.71LGLEE56 pKa = 4.22TSHH59 pKa = 7.38PEE61 pKa = 3.88LYY63 pKa = 10.78AKK65 pKa = 10.44LDD67 pKa = 3.68EE68 pKa = 5.6AYY70 pKa = 10.78HH71 pKa = 6.63DD72 pKa = 3.91MARR75 pKa = 11.84HH76 pKa = 5.5AEE78 pKa = 4.04YY79 pKa = 9.5MHH81 pKa = 6.38WLWEE85 pKa = 4.36GFDD88 pKa = 3.54NGYY91 pKa = 10.43YY92 pKa = 10.3EE93 pKa = 4.23YY94 pKa = 10.95DD95 pKa = 3.55EE96 pKa = 5.12EE97 pKa = 4.87EE98 pKa = 4.26LMDD101 pKa = 3.81YY102 pKa = 10.72CEE104 pKa = 4.61RR105 pKa = 11.84EE106 pKa = 3.87CGFNFEE112 pKa = 5.27FIEE115 pKa = 4.72EE116 pKa = 4.65DD117 pKa = 3.66YY118 pKa = 11.35LDD120 pKa = 4.04EE121 pKa = 5.26NGEE124 pKa = 3.73IDD126 pKa = 4.35EE127 pKa = 4.5EE128 pKa = 4.49SKK130 pKa = 10.99EE131 pKa = 4.19YY132 pKa = 10.86AKK134 pKa = 10.74SAAFHH139 pKa = 7.2DD140 pKa = 3.83WLDD143 pKa = 3.81DD144 pKa = 3.85YY145 pKa = 11.73VRR147 pKa = 11.84SLSDD151 pKa = 3.31DD152 pKa = 3.69DD153 pKa = 4.72AAEE156 pKa = 4.11FMRR159 pKa = 11.84DD160 pKa = 3.28HH161 pKa = 6.87MDD163 pKa = 3.43AEE165 pKa = 4.26VDD167 pKa = 3.45VDD169 pKa = 3.81EE170 pKa = 5.22VEE172 pKa = 4.44YY173 pKa = 10.92AVNVPEE179 pKa = 6.1DD180 pKa = 3.64IIKK183 pKa = 10.2KK184 pKa = 10.34AKK186 pKa = 9.73EE187 pKa = 3.49QDD189 pKa = 3.08

Molecular weight: 22.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I5I2D6|A0A1I5I2D6_9BACT Uroporphyrinogen decarboxylase OS=Prevotella sp. tf2-5 OX=1761889 GN=SAMN04487852_102283 PE=4 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2923

0

2923

1074683

29

4024

367.7

41.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.048 ± 0.045

1.355 ± 0.016

5.952 ± 0.031

6.486 ± 0.044

4.299 ± 0.027

6.921 ± 0.048

2.148 ± 0.024

6.746 ± 0.038

6.371 ± 0.043

8.67 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.988 ± 0.026

4.854 ± 0.037

3.66 ± 0.021

3.878 ± 0.033

4.676 ± 0.036

5.765 ± 0.035

5.854 ± 0.049

6.558 ± 0.033

1.358 ± 0.019

4.415 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski