Amino acid dipepetide frequency for Escherichia phage PGT2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.661AlaAla: 13.661 ± 1.783
0.776AlaCys: 0.776 ± 0.328
5.977AlaAsp: 5.977 ± 0.687
6.831AlaGlu: 6.831 ± 0.764
3.338AlaPhe: 3.338 ± 0.528
8.073AlaGly: 8.073 ± 0.719
1.242AlaHis: 1.242 ± 0.3
5.434AlaIle: 5.434 ± 0.589
5.434AlaLys: 5.434 ± 0.695
8.694AlaLeu: 8.694 ± 0.975
2.406AlaMet: 2.406 ± 0.388
5.511AlaAsn: 5.511 ± 1.308
2.95AlaPro: 2.95 ± 1.069
6.132AlaGln: 6.132 ± 1.002
4.114AlaArg: 4.114 ± 0.502
5.744AlaSer: 5.744 ± 0.836
7.219AlaThr: 7.219 ± 1.287
6.831AlaVal: 6.831 ± 0.902
1.397AlaTrp: 1.397 ± 0.29
3.803AlaTyr: 3.803 ± 0.463
0.0AlaXaa: 0.0 ± 0.0
Cys
0.854CysAla: 0.854 ± 0.308
0.155CysCys: 0.155 ± 0.097
0.543CysAsp: 0.543 ± 0.247
0.621CysGlu: 0.621 ± 0.264
0.233CysPhe: 0.233 ± 0.127
0.466CysGly: 0.466 ± 0.227
0.233CysHis: 0.233 ± 0.104
0.699CysIle: 0.699 ± 0.218
0.466CysLys: 0.466 ± 0.227
0.854CysLeu: 0.854 ± 0.333
0.155CysMet: 0.155 ± 0.11
0.388CysAsn: 0.388 ± 0.145
0.699CysPro: 0.699 ± 0.285
0.388CysGln: 0.388 ± 0.152
0.466CysArg: 0.466 ± 0.247
0.931CysSer: 0.931 ± 0.296
0.699CysThr: 0.699 ± 0.258
0.621CysVal: 0.621 ± 0.216
0.0CysTrp: 0.0 ± 0.0
0.233CysTyr: 0.233 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
6.287AspAla: 6.287 ± 0.775
0.776AspCys: 0.776 ± 0.217
4.347AspAsp: 4.347 ± 0.572
4.036AspGlu: 4.036 ± 0.443
2.794AspPhe: 2.794 ± 0.398
4.89AspGly: 4.89 ± 0.773
0.699AspHis: 0.699 ± 0.305
3.415AspIle: 3.415 ± 0.449
2.95AspLys: 2.95 ± 0.419
4.813AspLeu: 4.813 ± 0.636
1.785AspMet: 1.785 ± 0.305
2.173AspAsn: 2.173 ± 0.404
2.95AspPro: 2.95 ± 0.46
2.096AspGln: 2.096 ± 0.474
2.562AspArg: 2.562 ± 0.41
3.959AspSer: 3.959 ± 0.72
3.571AspThr: 3.571 ± 0.481
3.571AspVal: 3.571 ± 0.522
1.087AspTrp: 1.087 ± 0.267
1.708AspTyr: 1.708 ± 0.409
0.0AspXaa: 0.0 ± 0.0
Glu
6.598GluAla: 6.598 ± 0.876
0.466GluCys: 0.466 ± 0.196
3.182GluAsp: 3.182 ± 0.434
3.726GluGlu: 3.726 ± 0.665
2.95GluPhe: 2.95 ± 0.428
2.251GluGly: 2.251 ± 0.518
1.32GluHis: 1.32 ± 0.333
3.571GluIle: 3.571 ± 0.61
3.959GluLys: 3.959 ± 0.635
3.881GluLeu: 3.881 ± 0.458
1.32GluMet: 1.32 ± 0.368
1.785GluAsn: 1.785 ± 0.325
1.552GluPro: 1.552 ± 0.404
3.571GluGln: 3.571 ± 0.525
3.493GluArg: 3.493 ± 0.536
3.959GluSer: 3.959 ± 0.556
3.027GluThr: 3.027 ± 0.557
3.726GluVal: 3.726 ± 0.448
0.776GluTrp: 0.776 ± 0.217
2.717GluTyr: 2.717 ± 0.445
0.0GluXaa: 0.0 ± 0.0
Phe
2.018PheAla: 2.018 ± 0.329
0.388PheCys: 0.388 ± 0.168
2.562PheAsp: 2.562 ± 0.489
2.096PheGlu: 2.096 ± 0.368
1.009PhePhe: 1.009 ± 0.29
2.872PheGly: 2.872 ± 0.435
0.466PheHis: 0.466 ± 0.162
2.329PheIle: 2.329 ± 0.389
1.785PheLys: 1.785 ± 0.367
2.717PheLeu: 2.717 ± 0.509
1.087PheMet: 1.087 ± 0.299
1.475PheAsn: 1.475 ± 0.365
1.087PhePro: 1.087 ± 0.257
2.096PheGln: 2.096 ± 0.247
2.484PheArg: 2.484 ± 0.361
2.018PheSer: 2.018 ± 0.366
1.397PheThr: 1.397 ± 0.254
1.63PheVal: 1.63 ± 0.308
0.31PheTrp: 0.31 ± 0.171
1.397PheTyr: 1.397 ± 0.304
0.0PheXaa: 0.0 ± 0.0
Gly
5.822GlyAla: 5.822 ± 0.77
0.621GlyCys: 0.621 ± 0.278
5.356GlyAsp: 5.356 ± 0.522
2.484GlyGlu: 2.484 ± 0.287
2.562GlyPhe: 2.562 ± 0.497
6.831GlyGly: 6.831 ± 0.888
1.552GlyHis: 1.552 ± 0.333
3.959GlyIle: 3.959 ± 0.641
3.959GlyLys: 3.959 ± 0.569
5.356GlyLeu: 5.356 ± 0.687
2.096GlyMet: 2.096 ± 0.381
4.347GlyAsn: 4.347 ± 0.555
1.785GlyPro: 1.785 ± 0.329
2.794GlyGln: 2.794 ± 0.395
4.036GlyArg: 4.036 ± 0.605
4.192GlySer: 4.192 ± 0.637
6.132GlyThr: 6.132 ± 0.675
5.744GlyVal: 5.744 ± 0.8
0.931GlyTrp: 0.931 ± 0.299
3.26GlyTyr: 3.26 ± 0.506
0.0GlyXaa: 0.0 ± 0.0
His
1.397HisAla: 1.397 ± 0.361
0.31HisCys: 0.31 ± 0.121
1.475HisAsp: 1.475 ± 0.4
0.931HisGlu: 0.931 ± 0.253
0.699HisPhe: 0.699 ± 0.228
2.018HisGly: 2.018 ± 0.537
0.621HisHis: 0.621 ± 0.306
0.155HisIle: 0.155 ± 0.116
0.543HisLys: 0.543 ± 0.174
1.397HisLeu: 1.397 ± 0.324
0.621HisMet: 0.621 ± 0.181
0.621HisAsn: 0.621 ± 0.193
1.009HisPro: 1.009 ± 0.402
0.543HisGln: 0.543 ± 0.273
1.32HisArg: 1.32 ± 0.283
1.087HisSer: 1.087 ± 0.253
1.397HisThr: 1.397 ± 0.345
1.087HisVal: 1.087 ± 0.272
0.31HisTrp: 0.31 ± 0.157
0.543HisTyr: 0.543 ± 0.163
0.0HisXaa: 0.0 ± 0.0
Ile
4.269IleAla: 4.269 ± 0.452
1.087IleCys: 1.087 ± 0.295
3.415IleAsp: 3.415 ± 0.555
2.794IleGlu: 2.794 ± 0.504
1.087IlePhe: 1.087 ± 0.249
3.571IleGly: 3.571 ± 0.589
0.699IleHis: 0.699 ± 0.197
2.251IleIle: 2.251 ± 0.382
2.639IleLys: 2.639 ± 0.51
2.95IleLeu: 2.95 ± 0.594
1.397IleMet: 1.397 ± 0.325
3.105IleAsn: 3.105 ± 0.482
2.562IlePro: 2.562 ± 0.414
1.397IleGln: 1.397 ± 0.271
2.95IleArg: 2.95 ± 0.469
3.571IleSer: 3.571 ± 0.475
3.571IleThr: 3.571 ± 0.545
3.493IleVal: 3.493 ± 0.519
0.854IleTrp: 0.854 ± 0.289
1.32IleTyr: 1.32 ± 0.312
0.0IleXaa: 0.0 ± 0.0
Lys
6.753LysAla: 6.753 ± 0.683
0.155LysCys: 0.155 ± 0.125
3.26LysAsp: 3.26 ± 0.428
3.26LysGlu: 3.26 ± 0.475
2.251LysPhe: 2.251 ± 0.388
3.105LysGly: 3.105 ± 0.532
0.854LysHis: 0.854 ± 0.248
1.785LysIle: 1.785 ± 0.34
4.114LysLys: 4.114 ± 0.974
5.123LysLeu: 5.123 ± 0.909
1.785LysMet: 1.785 ± 0.29
2.096LysAsn: 2.096 ± 0.414
1.941LysPro: 1.941 ± 0.403
2.329LysGln: 2.329 ± 0.336
3.26LysArg: 3.26 ± 0.656
3.182LysSer: 3.182 ± 0.376
3.027LysThr: 3.027 ± 0.48
3.182LysVal: 3.182 ± 0.427
0.931LysTrp: 0.931 ± 0.224
1.552LysTyr: 1.552 ± 0.323
0.0LysXaa: 0.0 ± 0.0
Leu
6.675LeuAla: 6.675 ± 0.602
1.009LeuCys: 1.009 ± 0.282
4.813LeuAsp: 4.813 ± 0.655
4.114LeuGlu: 4.114 ± 0.591
2.406LeuPhe: 2.406 ± 0.442
5.589LeuGly: 5.589 ± 0.822
2.018LeuHis: 2.018 ± 0.501
4.347LeuIle: 4.347 ± 0.741
4.58LeuLys: 4.58 ± 0.636
6.598LeuLeu: 6.598 ± 0.564
1.63LeuMet: 1.63 ± 0.351
4.114LeuAsn: 4.114 ± 0.601
3.027LeuPro: 3.027 ± 0.488
3.415LeuGln: 3.415 ± 0.674
2.95LeuArg: 2.95 ± 0.297
7.296LeuSer: 7.296 ± 0.66
4.502LeuThr: 4.502 ± 0.584
5.278LeuVal: 5.278 ± 0.737
0.854LeuTrp: 0.854 ± 0.247
2.484LeuTyr: 2.484 ± 0.431
0.0LeuXaa: 0.0 ± 0.0
Met
2.562MetAla: 2.562 ± 0.328
0.078MetCys: 0.078 ± 0.075
1.63MetAsp: 1.63 ± 0.35
1.242MetGlu: 1.242 ± 0.329
1.087MetPhe: 1.087 ± 0.2
1.863MetGly: 1.863 ± 0.445
0.388MetHis: 0.388 ± 0.119
1.009MetIle: 1.009 ± 0.26
1.708MetLys: 1.708 ± 0.346
2.639MetLeu: 2.639 ± 0.488
0.699MetMet: 0.699 ± 0.344
1.397MetAsn: 1.397 ± 0.296
1.087MetPro: 1.087 ± 0.219
1.32MetGln: 1.32 ± 0.346
1.941MetArg: 1.941 ± 0.482
1.785MetSer: 1.785 ± 0.32
1.552MetThr: 1.552 ± 0.342
2.406MetVal: 2.406 ± 0.331
0.466MetTrp: 0.466 ± 0.202
0.931MetTyr: 0.931 ± 0.264
0.0MetXaa: 0.0 ± 0.0
Asn
4.58AsnAla: 4.58 ± 0.814
0.388AsnCys: 0.388 ± 0.213
1.63AsnAsp: 1.63 ± 0.348
2.95AsnGlu: 2.95 ± 0.383
1.475AsnPhe: 1.475 ± 0.36
3.803AsnGly: 3.803 ± 0.626
0.931AsnHis: 0.931 ± 0.289
2.95AsnIle: 2.95 ± 0.417
2.406AsnLys: 2.406 ± 0.367
3.648AsnLeu: 3.648 ± 0.592
1.785AsnMet: 1.785 ± 0.391
2.562AsnAsn: 2.562 ± 0.5
2.251AsnPro: 2.251 ± 0.333
1.941AsnGln: 1.941 ± 0.441
2.406AsnArg: 2.406 ± 0.407
2.562AsnSer: 2.562 ± 0.555
3.493AsnThr: 3.493 ± 0.507
2.251AsnVal: 2.251 ± 0.437
0.854AsnTrp: 0.854 ± 0.279
1.397AsnTyr: 1.397 ± 0.305
0.0AsnXaa: 0.0 ± 0.0
Pro
4.424ProAla: 4.424 ± 1.061
0.233ProCys: 0.233 ± 0.124
3.027ProAsp: 3.027 ± 0.535
3.105ProGlu: 3.105 ± 0.483
1.164ProPhe: 1.164 ± 0.261
2.639ProGly: 2.639 ± 0.51
0.388ProHis: 0.388 ± 0.146
1.397ProIle: 1.397 ± 0.257
1.63ProLys: 1.63 ± 0.453
2.95ProLeu: 2.95 ± 0.437
0.854ProMet: 0.854 ± 0.231
2.173ProAsn: 2.173 ± 0.419
1.32ProPro: 1.32 ± 0.359
2.251ProGln: 2.251 ± 0.567
0.931ProArg: 0.931 ± 0.297
2.173ProSer: 2.173 ± 0.486
2.872ProThr: 2.872 ± 0.431
3.105ProVal: 3.105 ± 0.529
0.388ProTrp: 0.388 ± 0.155
1.708ProTyr: 1.708 ± 0.235
0.0ProXaa: 0.0 ± 0.0
Gln
6.132GlnAla: 6.132 ± 1.191
0.078GlnCys: 0.078 ± 0.084
1.397GlnAsp: 1.397 ± 0.326
2.717GlnGlu: 2.717 ± 0.397
1.63GlnPhe: 1.63 ± 0.256
2.872GlnGly: 2.872 ± 0.553
0.699GlnHis: 0.699 ± 0.204
2.173GlnIle: 2.173 ± 0.433
1.941GlnLys: 1.941 ± 0.501
4.114GlnLeu: 4.114 ± 0.6
1.63GlnMet: 1.63 ± 0.289
1.63GlnAsn: 1.63 ± 0.432
1.785GlnPro: 1.785 ± 0.342
5.045GlnGln: 5.045 ± 1.184
3.493GlnArg: 3.493 ± 0.653
2.872GlnSer: 2.872 ± 0.567
2.329GlnThr: 2.329 ± 0.409
3.26GlnVal: 3.26 ± 0.566
0.466GlnTrp: 0.466 ± 0.146
1.785GlnTyr: 1.785 ± 0.4
0.0GlnXaa: 0.0 ± 0.0
Arg
5.045ArgAla: 5.045 ± 0.555
0.931ArgCys: 0.931 ± 0.332
3.571ArgAsp: 3.571 ± 0.507
4.424ArgGlu: 4.424 ± 0.644
2.096ArgPhe: 2.096 ± 0.485
3.803ArgGly: 3.803 ± 0.457
1.164ArgHis: 1.164 ± 0.286
3.415ArgIle: 3.415 ± 0.464
2.717ArgLys: 2.717 ± 0.585
3.415ArgLeu: 3.415 ± 0.572
2.251ArgMet: 2.251 ± 0.426
1.708ArgAsn: 1.708 ± 0.331
2.406ArgPro: 2.406 ± 0.375
2.096ArgGln: 2.096 ± 0.423
2.794ArgArg: 2.794 ± 0.529
2.329ArgSer: 2.329 ± 0.447
2.329ArgThr: 2.329 ± 0.48
3.105ArgVal: 3.105 ± 0.556
1.397ArgTrp: 1.397 ± 0.367
2.096ArgTyr: 2.096 ± 0.361
0.0ArgXaa: 0.0 ± 0.0
Ser
7.607SerAla: 7.607 ± 1.1
0.388SerCys: 0.388 ± 0.17
3.959SerAsp: 3.959 ± 0.583
3.338SerGlu: 3.338 ± 0.598
1.708SerPhe: 1.708 ± 0.298
4.657SerGly: 4.657 ± 0.629
1.009SerHis: 1.009 ± 0.264
2.872SerIle: 2.872 ± 0.383
3.493SerLys: 3.493 ± 0.526
4.968SerLeu: 4.968 ± 0.587
1.32SerMet: 1.32 ± 0.28
2.717SerAsn: 2.717 ± 0.49
3.105SerPro: 3.105 ± 0.644
2.251SerGln: 2.251 ± 0.637
3.726SerArg: 3.726 ± 0.36
4.114SerSer: 4.114 ± 0.64
5.434SerThr: 5.434 ± 0.964
4.89SerVal: 4.89 ± 0.689
0.621SerTrp: 0.621 ± 0.213
2.173SerTyr: 2.173 ± 0.394
0.0SerXaa: 0.0 ± 0.0
Thr
9.082ThrAla: 9.082 ± 2.335
0.31ThrCys: 0.31 ± 0.149
4.192ThrAsp: 4.192 ± 0.722
3.415ThrGlu: 3.415 ± 0.417
1.708ThrPhe: 1.708 ± 0.314
5.744ThrGly: 5.744 ± 0.693
1.475ThrHis: 1.475 ± 0.286
2.329ThrIle: 2.329 ± 0.43
4.269ThrLys: 4.269 ± 0.615
4.657ThrLeu: 4.657 ± 0.528
1.552ThrMet: 1.552 ± 0.338
2.872ThrAsn: 2.872 ± 0.392
3.105ThrPro: 3.105 ± 0.455
2.251ThrGln: 2.251 ± 0.436
3.648ThrArg: 3.648 ± 0.67
4.036ThrSer: 4.036 ± 0.417
4.89ThrThr: 4.89 ± 0.764
3.571ThrVal: 3.571 ± 0.5
0.699ThrTrp: 0.699 ± 0.27
2.329ThrTyr: 2.329 ± 0.427
0.0ThrXaa: 0.0 ± 0.0
Val
7.374ValAla: 7.374 ± 0.698
0.699ValCys: 0.699 ± 0.252
3.648ValAsp: 3.648 ± 0.505
4.036ValGlu: 4.036 ± 0.481
1.475ValPhe: 1.475 ± 0.276
5.511ValGly: 5.511 ± 0.584
1.63ValHis: 1.63 ± 0.399
2.639ValIle: 2.639 ± 0.377
2.794ValLys: 2.794 ± 0.435
4.036ValLeu: 4.036 ± 0.557
2.018ValMet: 2.018 ± 0.408
2.95ValAsn: 2.95 ± 0.383
2.872ValPro: 2.872 ± 0.501
3.571ValGln: 3.571 ± 0.516
4.269ValArg: 4.269 ± 0.714
4.58ValSer: 4.58 ± 0.572
4.813ValThr: 4.813 ± 0.545
5.123ValVal: 5.123 ± 0.827
0.776ValTrp: 0.776 ± 0.158
2.018ValTyr: 2.018 ± 0.429
0.0ValXaa: 0.0 ± 0.0
Trp
0.776TrpAla: 0.776 ± 0.223
0.31TrpCys: 0.31 ± 0.138
1.087TrpAsp: 1.087 ± 0.239
0.621TrpGlu: 0.621 ± 0.199
0.388TrpPhe: 0.388 ± 0.186
0.621TrpGly: 0.621 ± 0.201
0.155TrpHis: 0.155 ± 0.107
0.388TrpIle: 0.388 ± 0.142
0.543TrpLys: 0.543 ± 0.202
1.552TrpLeu: 1.552 ± 0.386
0.388TrpMet: 0.388 ± 0.13
0.543TrpAsn: 0.543 ± 0.177
0.388TrpPro: 0.388 ± 0.157
1.087TrpGln: 1.087 ± 0.215
0.543TrpArg: 0.543 ± 0.202
1.32TrpSer: 1.32 ± 0.297
0.854TrpThr: 0.854 ± 0.277
1.087TrpVal: 1.087 ± 0.273
0.233TrpTrp: 0.233 ± 0.128
0.854TrpTyr: 0.854 ± 0.281
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.648TyrAla: 3.648 ± 0.459
0.543TyrCys: 0.543 ± 0.239
1.397TyrAsp: 1.397 ± 0.271
1.087TyrGlu: 1.087 ± 0.278
1.164TyrPhe: 1.164 ± 0.266
2.639TyrGly: 2.639 ± 0.377
0.543TyrHis: 0.543 ± 0.153
1.863TyrIle: 1.863 ± 0.313
2.018TyrLys: 2.018 ± 0.378
3.338TyrLeu: 3.338 ± 0.482
0.931TyrMet: 0.931 ± 0.228
2.096TyrAsn: 2.096 ± 0.341
0.854TyrPro: 0.854 ± 0.313
1.475TyrGln: 1.475 ± 0.316
1.785TyrArg: 1.785 ± 0.406
2.639TyrSer: 2.639 ± 0.472
3.027TyrThr: 3.027 ± 0.46
2.872TyrVal: 2.872 ± 0.497
0.388TyrTrp: 0.388 ± 0.192
1.397TyrTyr: 1.397 ± 0.295
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (12884 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski