Salmonella phage SI22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Eganvirus; unclassified Eganvirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C0CGZ3|A0A5C0CGZ3_9CAUD Tail protein OS=Salmonella phage SI22 OX=2592209 PE=4 SV=1
MM1 pKa = 7.4QGDD4 pKa = 4.08TLDD7 pKa = 4.24VICARR12 pKa = 11.84YY13 pKa = 9.04YY14 pKa = 10.75GRR16 pKa = 11.84TEE18 pKa = 4.12GVVEE22 pKa = 4.42TVLQANPGLSEE33 pKa = 4.6LGVILPHH40 pKa = 6.21GTAIDD45 pKa = 4.16LPDD48 pKa = 3.87VPSSPVTEE56 pKa = 4.95TINLWEE62 pKa = 4.05

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C0CH54|A0A5C0CH54_9CAUD DUF2724 domain-containing protein OS=Salmonella phage SI22 OX=2592209 PE=4 SV=1
MM1 pKa = 6.76TTLDD5 pKa = 4.14PRR7 pKa = 11.84QLWRR11 pKa = 11.84RR12 pKa = 11.84TARR15 pKa = 11.84TGKK18 pKa = 10.2SDD20 pKa = 3.01ACKK23 pKa = 10.13TMHH26 pKa = 7.22LLHH29 pKa = 7.04ARR31 pKa = 11.84LKK33 pKa = 9.09NGKK36 pKa = 8.57FAEE39 pKa = 4.39EE40 pKa = 3.84WHH42 pKa = 6.75KK43 pKa = 10.64KK44 pKa = 7.85TGIHH48 pKa = 6.53GAGLNQVYY56 pKa = 10.25

Molecular weight: 6.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

9566

39

814

212.6

23.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.391 ± 0.513

0.972 ± 0.137

6.251 ± 0.238

5.969 ± 0.337

3.084 ± 0.259

6.941 ± 0.46

1.955 ± 0.225

5.415 ± 0.195

5.614 ± 0.307

9.168 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.158

4.077 ± 0.222

4.087 ± 0.241

3.805 ± 0.316

6.293 ± 0.451

6.23 ± 0.255

6.324 ± 0.326

6.586 ± 0.33

1.662 ± 0.165

2.655 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski