Amino acid dipepetide frequency for Salmonella phage SI22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.141AlaAla: 10.141 ± 1.872
1.464AlaCys: 1.464 ± 0.482
6.9AlaAsp: 6.9 ± 1.047
6.482AlaGlu: 6.482 ± 0.907
2.509AlaPhe: 2.509 ± 0.476
8.05AlaGly: 8.05 ± 1.058
1.882AlaHis: 1.882 ± 0.373
5.227AlaIle: 5.227 ± 0.797
6.273AlaLys: 6.273 ± 0.606
9.827AlaLeu: 9.827 ± 0.878
2.614AlaMet: 2.614 ± 0.428
2.823AlaAsn: 2.823 ± 0.794
5.436AlaPro: 5.436 ± 0.929
3.868AlaGln: 3.868 ± 0.772
5.436AlaArg: 5.436 ± 0.953
7.214AlaSer: 7.214 ± 0.799
6.482AlaThr: 6.482 ± 1.121
7.005AlaVal: 7.005 ± 0.701
1.673AlaTrp: 1.673 ± 0.475
2.614AlaTyr: 2.614 ± 0.567
0.0AlaXaa: 0.0 ± 0.0
Cys
1.045CysAla: 1.045 ± 0.39
0.105CysCys: 0.105 ± 0.093
0.523CysAsp: 0.523 ± 0.231
0.627CysGlu: 0.627 ± 0.276
0.314CysPhe: 0.314 ± 0.225
0.314CysGly: 0.314 ± 0.182
0.209CysHis: 0.209 ± 0.16
0.627CysIle: 0.627 ± 0.219
0.732CysLys: 0.732 ± 0.261
0.418CysLeu: 0.418 ± 0.175
0.209CysMet: 0.209 ± 0.138
0.732CysAsn: 0.732 ± 0.225
0.105CysPro: 0.105 ± 0.106
0.418CysGln: 0.418 ± 0.23
0.941CysArg: 0.941 ± 0.347
0.314CysSer: 0.314 ± 0.185
0.836CysThr: 0.836 ± 0.262
0.836CysVal: 0.836 ± 0.308
0.105CysTrp: 0.105 ± 0.101
0.314CysTyr: 0.314 ± 0.165
0.0CysXaa: 0.0 ± 0.0
Asp
6.482AspAla: 6.482 ± 0.735
0.418AspCys: 0.418 ± 0.212
4.6AspAsp: 4.6 ± 0.636
4.077AspGlu: 4.077 ± 0.595
2.927AspPhe: 2.927 ± 0.613
5.959AspGly: 5.959 ± 0.595
0.523AspHis: 0.523 ± 0.219
4.705AspIle: 4.705 ± 0.728
3.346AspLys: 3.346 ± 0.597
4.077AspLeu: 4.077 ± 0.664
1.255AspMet: 1.255 ± 0.374
2.718AspAsn: 2.718 ± 0.706
2.3AspPro: 2.3 ± 0.504
1.568AspGln: 1.568 ± 0.446
2.823AspArg: 2.823 ± 0.555
2.927AspSer: 2.927 ± 0.703
4.182AspThr: 4.182 ± 0.618
4.391AspVal: 4.391 ± 0.795
1.15AspTrp: 1.15 ± 0.352
2.091AspTyr: 2.091 ± 0.515
0.0AspXaa: 0.0 ± 0.0
Glu
6.064GluAla: 6.064 ± 0.888
1.045GluCys: 1.045 ± 0.366
2.405GluAsp: 2.405 ± 0.619
2.614GluGlu: 2.614 ± 0.552
1.673GluPhe: 1.673 ± 0.396
2.823GluGly: 2.823 ± 0.563
1.255GluHis: 1.255 ± 0.337
3.973GluIle: 3.973 ± 0.634
2.927GluLys: 2.927 ± 0.527
8.991GluLeu: 8.991 ± 0.948
1.986GluMet: 1.986 ± 0.417
3.346GluAsn: 3.346 ± 0.702
2.718GluPro: 2.718 ± 0.454
2.405GluGln: 2.405 ± 0.582
3.241GluArg: 3.241 ± 0.576
3.868GluSer: 3.868 ± 0.609
3.241GluThr: 3.241 ± 0.525
2.718GluVal: 2.718 ± 0.491
0.732GluTrp: 0.732 ± 0.27
1.673GluTyr: 1.673 ± 0.462
0.0GluXaa: 0.0 ± 0.0
Phe
2.823PheAla: 2.823 ± 0.605
0.523PheCys: 0.523 ± 0.232
1.359PheAsp: 1.359 ± 0.493
2.091PheGlu: 2.091 ± 0.412
1.15PhePhe: 1.15 ± 0.325
1.882PheGly: 1.882 ± 0.344
0.941PheHis: 0.941 ± 0.25
1.15PheIle: 1.15 ± 0.388
2.405PheLys: 2.405 ± 0.414
1.882PheLeu: 1.882 ± 0.492
0.836PheMet: 0.836 ± 0.233
1.673PheAsn: 1.673 ± 0.412
1.045PhePro: 1.045 ± 0.31
1.255PheGln: 1.255 ± 0.332
1.673PheArg: 1.673 ± 0.392
2.718PheSer: 2.718 ± 0.584
2.3PheThr: 2.3 ± 0.573
1.673PheVal: 1.673 ± 0.455
0.732PheTrp: 0.732 ± 0.283
0.732PheTyr: 0.732 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
6.168GlyAla: 6.168 ± 0.678
1.045GlyCys: 1.045 ± 0.426
4.809GlyAsp: 4.809 ± 0.679
5.436GlyGlu: 5.436 ± 0.758
2.3GlyPhe: 2.3 ± 0.519
5.646GlyGly: 5.646 ± 1.324
1.15GlyHis: 1.15 ± 0.25
4.286GlyIle: 4.286 ± 0.765
4.914GlyLys: 4.914 ± 0.61
5.123GlyLeu: 5.123 ± 0.741
2.196GlyMet: 2.196 ± 0.52
2.823GlyAsn: 2.823 ± 0.471
0.418GlyPro: 0.418 ± 0.213
2.091GlyGln: 2.091 ± 0.551
3.659GlyArg: 3.659 ± 0.714
3.659GlySer: 3.659 ± 0.599
4.182GlyThr: 4.182 ± 0.767
6.168GlyVal: 6.168 ± 1.034
1.15GlyTrp: 1.15 ± 0.357
2.196GlyTyr: 2.196 ± 0.512
0.0GlyXaa: 0.0 ± 0.0
His
1.882HisAla: 1.882 ± 0.565
0.418HisCys: 0.418 ± 0.183
0.732HisAsp: 0.732 ± 0.266
0.836HisGlu: 0.836 ± 0.212
0.627HisPhe: 0.627 ± 0.302
1.359HisGly: 1.359 ± 0.38
0.523HisHis: 0.523 ± 0.214
1.045HisIle: 1.045 ± 0.275
0.836HisLys: 0.836 ± 0.27
1.673HisLeu: 1.673 ± 0.353
0.732HisMet: 0.732 ± 0.27
1.255HisAsn: 1.255 ± 0.395
0.941HisPro: 0.941 ± 0.341
0.836HisGln: 0.836 ± 0.256
1.568HisArg: 1.568 ± 0.41
0.941HisSer: 0.941 ± 0.304
1.045HisThr: 1.045 ± 0.335
0.732HisVal: 0.732 ± 0.268
0.418HisTrp: 0.418 ± 0.243
1.045HisTyr: 1.045 ± 0.313
0.0HisXaa: 0.0 ± 0.0
Ile
5.75IleAla: 5.75 ± 0.966
0.314IleCys: 0.314 ± 0.183
5.018IleAsp: 5.018 ± 0.657
2.405IleGlu: 2.405 ± 0.399
1.986IlePhe: 1.986 ± 0.45
3.764IleGly: 3.764 ± 0.677
1.15IleHis: 1.15 ± 0.358
2.405IleIle: 2.405 ± 0.513
2.509IleLys: 2.509 ± 0.558
2.3IleLeu: 2.3 ± 0.372
1.045IleMet: 1.045 ± 0.272
2.614IleAsn: 2.614 ± 0.482
2.091IlePro: 2.091 ± 0.49
1.673IleGln: 1.673 ± 0.401
5.75IleArg: 5.75 ± 0.803
4.809IleSer: 4.809 ± 0.815
3.868IleThr: 3.868 ± 0.723
3.346IleVal: 3.346 ± 0.535
0.523IleTrp: 0.523 ± 0.222
0.836IleTyr: 0.836 ± 0.301
0.0IleXaa: 0.0 ± 0.0
Lys
6.377LysAla: 6.377 ± 0.745
0.209LysCys: 0.209 ± 0.142
2.509LysAsp: 2.509 ± 0.469
3.032LysGlu: 3.032 ± 0.66
1.15LysPhe: 1.15 ± 0.289
3.659LysGly: 3.659 ± 0.53
1.359LysHis: 1.359 ± 0.336
1.986LysIle: 1.986 ± 0.45
3.346LysLys: 3.346 ± 0.755
5.436LysLeu: 5.436 ± 0.748
0.836LysMet: 0.836 ± 0.317
2.718LysAsn: 2.718 ± 0.618
3.241LysPro: 3.241 ± 0.634
1.777LysGln: 1.777 ± 0.35
3.555LysArg: 3.555 ± 0.536
3.346LysSer: 3.346 ± 0.504
4.182LysThr: 4.182 ± 0.576
3.555LysVal: 3.555 ± 0.688
1.568LysTrp: 1.568 ± 0.444
2.3LysTyr: 2.3 ± 0.615
0.0LysXaa: 0.0 ± 0.0
Leu
10.141LeuAla: 10.141 ± 0.918
0.836LeuCys: 0.836 ± 0.279
6.482LeuAsp: 6.482 ± 0.954
4.705LeuGlu: 4.705 ± 0.601
2.405LeuPhe: 2.405 ± 0.668
5.75LeuGly: 5.75 ± 0.86
2.091LeuHis: 2.091 ± 0.366
4.286LeuIle: 4.286 ± 0.494
4.914LeuLys: 4.914 ± 0.676
5.855LeuLeu: 5.855 ± 0.902
2.091LeuMet: 2.091 ± 0.552
4.286LeuAsn: 4.286 ± 0.608
3.764LeuPro: 3.764 ± 0.629
3.973LeuGln: 3.973 ± 0.55
5.75LeuArg: 5.75 ± 0.845
7.946LeuSer: 7.946 ± 1.093
7.841LeuThr: 7.841 ± 1.253
4.286LeuVal: 4.286 ± 0.582
1.673LeuTrp: 1.673 ± 0.443
2.614LeuTyr: 2.614 ± 0.529
0.0LeuXaa: 0.0 ± 0.0
Met
3.764MetAla: 3.764 ± 0.478
0.105MetCys: 0.105 ± 0.103
0.627MetAsp: 0.627 ± 0.249
1.359MetGlu: 1.359 ± 0.358
1.255MetPhe: 1.255 ± 0.376
0.732MetGly: 0.732 ± 0.295
0.523MetHis: 0.523 ± 0.253
0.732MetIle: 0.732 ± 0.288
0.836MetLys: 0.836 ± 0.291
2.718MetLeu: 2.718 ± 0.496
0.418MetMet: 0.418 ± 0.184
1.15MetAsn: 1.15 ± 0.326
0.627MetPro: 0.627 ± 0.254
1.568MetGln: 1.568 ± 0.396
1.777MetArg: 1.777 ± 0.459
2.196MetSer: 2.196 ± 0.428
2.091MetThr: 2.091 ± 0.477
1.986MetVal: 1.986 ± 0.399
0.209MetTrp: 0.209 ± 0.145
0.523MetTyr: 0.523 ± 0.217
0.0MetXaa: 0.0 ± 0.0
Asn
3.764AsnAla: 3.764 ± 0.947
0.209AsnCys: 0.209 ± 0.152
1.777AsnAsp: 1.777 ± 0.329
2.405AsnGlu: 2.405 ± 0.474
1.15AsnPhe: 1.15 ± 0.323
4.496AsnGly: 4.496 ± 0.917
0.314AsnHis: 0.314 ± 0.217
2.927AsnIle: 2.927 ± 0.663
2.509AsnLys: 2.509 ± 0.523
3.45AsnLeu: 3.45 ± 0.654
1.255AsnMet: 1.255 ± 0.383
2.091AsnAsn: 2.091 ± 0.509
1.777AsnPro: 1.777 ± 0.429
1.15AsnGln: 1.15 ± 0.363
2.509AsnArg: 2.509 ± 0.542
2.718AsnSer: 2.718 ± 0.518
2.196AsnThr: 2.196 ± 0.515
2.927AsnVal: 2.927 ± 0.426
0.314AsnTrp: 0.314 ± 0.175
0.836AsnTyr: 0.836 ± 0.224
0.0AsnXaa: 0.0 ± 0.0
Pro
4.6ProAla: 4.6 ± 0.691
0.209ProCys: 0.209 ± 0.135
3.868ProAsp: 3.868 ± 0.726
3.764ProGlu: 3.764 ± 0.555
0.941ProPhe: 0.941 ± 0.415
2.196ProGly: 2.196 ± 0.569
1.045ProHis: 1.045 ± 0.429
2.091ProIle: 2.091 ± 0.38
2.405ProLys: 2.405 ± 0.535
3.973ProLeu: 3.973 ± 0.621
0.314ProMet: 0.314 ± 0.166
1.045ProAsn: 1.045 ± 0.317
1.777ProPro: 1.777 ± 0.357
1.15ProGln: 1.15 ± 0.411
2.3ProArg: 2.3 ± 0.538
2.509ProSer: 2.509 ± 0.547
1.045ProThr: 1.045 ± 0.316
4.286ProVal: 4.286 ± 0.741
0.418ProTrp: 0.418 ± 0.2
0.941ProTyr: 0.941 ± 0.329
0.0ProXaa: 0.0 ± 0.0
Gln
4.077GlnAla: 4.077 ± 1.197
0.0GlnCys: 0.0 ± 0.0
2.196GlnAsp: 2.196 ± 0.633
1.673GlnGlu: 1.673 ± 0.463
1.777GlnPhe: 1.777 ± 0.453
1.464GlnGly: 1.464 ± 0.298
0.627GlnHis: 0.627 ± 0.187
2.091GlnIle: 2.091 ± 0.616
2.823GlnLys: 2.823 ± 0.455
4.705GlnLeu: 4.705 ± 0.818
0.627GlnMet: 0.627 ± 0.274
1.15GlnAsn: 1.15 ± 0.402
1.255GlnPro: 1.255 ± 0.268
1.882GlnGln: 1.882 ± 0.541
3.136GlnArg: 3.136 ± 0.458
2.718GlnSer: 2.718 ± 0.656
2.196GlnThr: 2.196 ± 0.465
1.673GlnVal: 1.673 ± 0.374
0.836GlnTrp: 0.836 ± 0.314
1.15GlnTyr: 1.15 ± 0.384
0.0GlnXaa: 0.0 ± 0.0
Arg
4.914ArgAla: 4.914 ± 0.698
0.627ArgCys: 0.627 ± 0.264
3.032ArgAsp: 3.032 ± 0.415
4.6ArgGlu: 4.6 ± 0.767
1.568ArgPhe: 1.568 ± 0.472
3.241ArgGly: 3.241 ± 0.797
1.673ArgHis: 1.673 ± 0.392
3.45ArgIle: 3.45 ± 0.794
4.391ArgLys: 4.391 ± 0.522
7.214ArgLeu: 7.214 ± 0.947
1.986ArgMet: 1.986 ± 0.498
2.3ArgAsn: 2.3 ± 0.474
2.3ArgPro: 2.3 ± 0.532
2.927ArgGln: 2.927 ± 0.619
5.436ArgArg: 5.436 ± 0.941
2.614ArgSer: 2.614 ± 0.491
3.45ArgThr: 3.45 ± 0.579
4.809ArgVal: 4.809 ± 0.684
1.045ArgTrp: 1.045 ± 0.357
1.359ArgTyr: 1.359 ± 0.308
0.0ArgXaa: 0.0 ± 0.0
Ser
7.423SerAla: 7.423 ± 0.868
0.418SerCys: 0.418 ± 0.251
4.705SerAsp: 4.705 ± 0.645
4.496SerGlu: 4.496 ± 0.595
2.091SerPhe: 2.091 ± 0.422
4.914SerGly: 4.914 ± 0.805
1.045SerHis: 1.045 ± 0.467
2.614SerIle: 2.614 ± 0.373
3.241SerLys: 3.241 ± 0.621
5.855SerLeu: 5.855 ± 0.756
1.568SerMet: 1.568 ± 0.427
2.614SerAsn: 2.614 ± 0.602
2.509SerPro: 2.509 ± 0.548
2.509SerGln: 2.509 ± 0.423
4.182SerArg: 4.182 ± 0.733
2.196SerSer: 2.196 ± 0.559
3.032SerThr: 3.032 ± 0.548
4.496SerVal: 4.496 ± 0.528
1.045SerTrp: 1.045 ± 0.355
1.359SerTyr: 1.359 ± 0.426
0.0SerXaa: 0.0 ± 0.0
Thr
7.527ThrAla: 7.527 ± 1.059
0.732ThrCys: 0.732 ± 0.267
4.705ThrAsp: 4.705 ± 0.823
2.196ThrGlu: 2.196 ± 0.525
1.045ThrPhe: 1.045 ± 0.3
6.168ThrGly: 6.168 ± 0.782
0.627ThrHis: 0.627 ± 0.221
3.764ThrIle: 3.764 ± 0.648
2.614ThrLys: 2.614 ± 0.52
8.782ThrLeu: 8.782 ± 0.982
1.986ThrMet: 1.986 ± 0.392
1.568ThrAsn: 1.568 ± 0.341
3.032ThrPro: 3.032 ± 0.463
2.196ThrGln: 2.196 ± 0.45
3.136ThrArg: 3.136 ± 0.606
2.823ThrSer: 2.823 ± 0.632
3.868ThrThr: 3.868 ± 0.686
4.286ThrVal: 4.286 ± 0.666
1.045ThrTrp: 1.045 ± 0.357
1.15ThrTyr: 1.15 ± 0.273
0.0ThrXaa: 0.0 ± 0.0
Val
6.796ValAla: 6.796 ± 0.727
0.836ValCys: 0.836 ± 0.339
3.45ValAsp: 3.45 ± 0.565
4.391ValGlu: 4.391 ± 0.695
2.614ValPhe: 2.614 ± 0.526
4.286ValGly: 4.286 ± 0.683
0.836ValHis: 0.836 ± 0.296
4.391ValIle: 4.391 ± 0.762
3.973ValLys: 3.973 ± 0.62
4.6ValLeu: 4.6 ± 0.63
1.986ValMet: 1.986 ± 0.448
2.823ValAsn: 2.823 ± 0.474
2.823ValPro: 2.823 ± 0.448
2.405ValGln: 2.405 ± 0.587
2.614ValArg: 2.614 ± 0.491
4.705ValSer: 4.705 ± 0.782
5.332ValThr: 5.332 ± 0.592
4.077ValVal: 4.077 ± 0.761
1.359ValTrp: 1.359 ± 0.346
1.568ValTyr: 1.568 ± 0.342
0.0ValXaa: 0.0 ± 0.0
Trp
1.673TrpAla: 1.673 ± 0.4
0.0TrpCys: 0.0 ± 0.0
0.732TrpAsp: 0.732 ± 0.317
1.15TrpGlu: 1.15 ± 0.31
0.627TrpPhe: 0.627 ± 0.275
0.732TrpGly: 0.732 ± 0.323
1.255TrpHis: 1.255 ± 0.34
0.732TrpIle: 0.732 ± 0.289
0.523TrpLys: 0.523 ± 0.242
3.032TrpLeu: 3.032 ± 0.795
0.209TrpMet: 0.209 ± 0.138
0.314TrpAsn: 0.314 ± 0.209
1.15TrpPro: 1.15 ± 0.378
0.627TrpGln: 0.627 ± 0.25
1.568TrpArg: 1.568 ± 0.392
0.523TrpSer: 0.523 ± 0.228
0.314TrpThr: 0.314 ± 0.17
0.523TrpVal: 0.523 ± 0.288
0.418TrpTrp: 0.418 ± 0.172
0.523TrpTyr: 0.523 ± 0.206
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.509TyrAla: 2.509 ± 0.559
0.209TyrCys: 0.209 ± 0.122
2.091TyrAsp: 2.091 ± 0.356
1.359TyrGlu: 1.359 ± 0.379
0.732TyrPhe: 0.732 ± 0.337
1.986TyrGly: 1.986 ± 0.425
0.523TyrHis: 0.523 ± 0.252
1.673TyrIle: 1.673 ± 0.41
0.627TyrLys: 0.627 ± 0.242
1.777TyrLeu: 1.777 ± 0.375
0.941TyrMet: 0.941 ± 0.261
0.732TyrAsn: 0.732 ± 0.221
1.568TyrPro: 1.568 ± 0.365
1.777TyrGln: 1.777 ± 0.393
1.882TyrArg: 1.882 ± 0.417
1.568TyrSer: 1.568 ± 0.474
1.568TyrThr: 1.568 ± 0.388
2.091TyrVal: 2.091 ± 0.468
0.209TyrTrp: 0.209 ± 0.122
0.732TyrTyr: 0.732 ± 0.305
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (9566 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski