Vibrio vulnificus (strain YJ016)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; Vibrio vulnificus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4990 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7MJW6|Q7MJW6_VIBVY Sodium/alanine symporter OS=Vibrio vulnificus (strain YJ016) OX=196600 GN=VV2044 PE=3 SV=1
MM1 pKa = 7.66NGLTKK6 pKa = 10.49SAISSVAAALLLAGCGGSDD25 pKa = 3.34GGKK28 pKa = 6.87TANVSFYY35 pKa = 11.37VSDD38 pKa = 4.16APVDD42 pKa = 3.92SAQSVSVGFTQVEE55 pKa = 4.24LVDD58 pKa = 3.92NEE60 pKa = 4.38GNSVFLDD67 pKa = 3.68VVPSTGVDD75 pKa = 3.47YY76 pKa = 11.36QKK78 pKa = 10.69IDD80 pKa = 3.53LLDD83 pKa = 3.69YY84 pKa = 11.06QGTDD88 pKa = 2.92SVLMVSTQPVPVGSYY103 pKa = 10.45KK104 pKa = 10.55EE105 pKa = 4.8LILHH109 pKa = 5.24VTDD112 pKa = 4.01EE113 pKa = 4.34NGVNYY118 pKa = 10.33VIDD121 pKa = 4.14EE122 pKa = 4.46NGQQPLKK129 pKa = 10.74QPSNKK134 pKa = 9.78LKK136 pKa = 10.76LGSFDD141 pKa = 3.55VTSEE145 pKa = 3.69AVQLYY150 pKa = 9.35TIEE153 pKa = 4.29FDD155 pKa = 3.6LRR157 pKa = 11.84QSLVMRR163 pKa = 11.84GNLGSNNGYY172 pKa = 9.04ILKK175 pKa = 9.28PHH177 pKa = 6.29GVSIVSNASAVSISGNVDD195 pKa = 3.41DD196 pKa = 6.03NLLTGDD202 pKa = 4.52EE203 pKa = 4.59SCTADD208 pKa = 3.27SQAFIYY214 pKa = 10.1LYY216 pKa = 10.14EE217 pKa = 5.05GEE219 pKa = 4.55GHH221 pKa = 7.36DD222 pKa = 4.25SANLIDD228 pKa = 6.13LVDD231 pKa = 4.0EE232 pKa = 5.31DD233 pKa = 5.47DD234 pKa = 5.76PEE236 pKa = 5.51FDD238 pKa = 3.52SQNPVPEE245 pKa = 4.25NAVKK249 pKa = 10.33PIASAGLDD257 pKa = 3.48EE258 pKa = 4.77NNNYY262 pKa = 10.45AFGFLNAGTYY272 pKa = 8.29TVAFSCNAGDD282 pKa = 5.1DD283 pKa = 4.14NPIQYY288 pKa = 10.48DD289 pKa = 3.65GTAIPLPTGQVGTTTLAAGEE309 pKa = 4.37NGTIDD314 pKa = 4.51FVPAPP319 pKa = 3.98

Molecular weight: 33.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q7MNM8|SYI_VIBVY Isoleucine--tRNA ligase OS=Vibrio vulnificus (strain YJ016) OX=196600 GN=ileS PE=3 SV=1
MM1 pKa = 7.49LRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84VQSKK9 pKa = 10.38RR10 pKa = 11.84LNKK13 pKa = 8.79TVAANRR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 10.13RR22 pKa = 11.84KK23 pKa = 8.96LANTKK28 pKa = 10.29RR29 pKa = 11.84KK30 pKa = 9.0ISARR34 pKa = 11.84NRR36 pKa = 11.84IFVHH40 pKa = 6.11LVNHH44 pKa = 6.98AA45 pKa = 3.49

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4990

0

4990

1549674

34

5206

310.6

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.82 ± 0.038

1.069 ± 0.014

5.269 ± 0.031

6.365 ± 0.041

4.184 ± 0.025

6.691 ± 0.032

2.363 ± 0.017

6.067 ± 0.028

5.169 ± 0.031

10.476 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.017

4.117 ± 0.024

3.852 ± 0.025

4.901 ± 0.03

4.682 ± 0.03

6.551 ± 0.028

5.271 ± 0.031

7.043 ± 0.031

1.292 ± 0.015

3.074 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski