Amino acid dipepetide frequency for Pedobacter nyackensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.346AlaAla: 6.346 ± 0.085
0.622AlaCys: 0.622 ± 0.02
4.262AlaAsp: 4.262 ± 0.048
4.518AlaGlu: 4.518 ± 0.059
3.437AlaPhe: 3.437 ± 0.052
5.527AlaGly: 5.527 ± 0.075
1.151AlaHis: 1.151 ± 0.021
5.458AlaIle: 5.458 ± 0.062
4.933AlaLys: 4.933 ± 0.06
6.864AlaLeu: 6.864 ± 0.072
1.668AlaMet: 1.668 ± 0.035
4.05AlaAsn: 4.05 ± 0.055
2.362AlaPro: 2.362 ± 0.044
2.807AlaGln: 2.807 ± 0.036
2.319AlaArg: 2.319 ± 0.035
4.675AlaSer: 4.675 ± 0.058
4.338AlaThr: 4.338 ± 0.082
4.79AlaVal: 4.79 ± 0.056
0.802AlaTrp: 0.802 ± 0.024
3.08AlaTyr: 3.08 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
0.467CysAla: 0.467 ± 0.018
0.106CysCys: 0.106 ± 0.008
0.354CysAsp: 0.354 ± 0.013
0.365CysGlu: 0.365 ± 0.018
0.452CysPhe: 0.452 ± 0.015
0.599CysGly: 0.599 ± 0.021
0.184CysHis: 0.184 ± 0.011
0.579CysIle: 0.579 ± 0.018
0.576CysLys: 0.576 ± 0.018
0.708CysLeu: 0.708 ± 0.022
0.167CysMet: 0.167 ± 0.01
0.381CysAsn: 0.381 ± 0.018
0.277CysPro: 0.277 ± 0.014
0.197CysGln: 0.197 ± 0.011
0.301CysArg: 0.301 ± 0.015
0.507CysSer: 0.507 ± 0.019
0.427CysThr: 0.427 ± 0.015
0.409CysVal: 0.409 ± 0.015
0.07CysTrp: 0.07 ± 0.007
0.304CysTyr: 0.304 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
3.939AspAla: 3.939 ± 0.046
0.365AspCys: 0.365 ± 0.013
2.368AspAsp: 2.368 ± 0.035
3.226AspGlu: 3.226 ± 0.052
3.116AspPhe: 3.116 ± 0.046
4.003AspGly: 4.003 ± 0.059
0.996AspHis: 0.996 ± 0.024
3.767AspIle: 3.767 ± 0.046
4.027AspLys: 4.027 ± 0.048
5.259AspLeu: 5.259 ± 0.055
1.196AspMet: 1.196 ± 0.027
2.676AspAsn: 2.676 ± 0.041
2.171AspPro: 2.171 ± 0.04
2.041AspGln: 2.041 ± 0.033
2.077AspArg: 2.077 ± 0.035
2.639AspSer: 2.639 ± 0.042
2.337AspThr: 2.337 ± 0.043
3.532AspVal: 3.532 ± 0.049
0.816AspTrp: 0.816 ± 0.021
2.512AspTyr: 2.512 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
4.205GluAla: 4.205 ± 0.061
0.307GluCys: 0.307 ± 0.012
2.625GluAsp: 2.625 ± 0.047
3.594GluGlu: 3.594 ± 0.053
2.419GluPhe: 2.419 ± 0.035
3.543GluGly: 3.543 ± 0.043
1.027GluHis: 1.027 ± 0.029
4.358GluIle: 4.358 ± 0.063
4.601GluLys: 4.601 ± 0.061
5.678GluLeu: 5.678 ± 0.071
1.456GluMet: 1.456 ± 0.029
3.407GluAsn: 3.407 ± 0.042
1.535GluPro: 1.535 ± 0.03
2.381GluGln: 2.381 ± 0.046
2.457GluArg: 2.457 ± 0.043
2.96GluSer: 2.96 ± 0.039
2.946GluThr: 2.946 ± 0.038
3.855GluVal: 3.855 ± 0.047
0.661GluTrp: 0.661 ± 0.019
1.946GluTyr: 1.946 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
3.177PheAla: 3.177 ± 0.042
0.413PheCys: 0.413 ± 0.015
2.825PheAsp: 2.825 ± 0.047
2.733PheGlu: 2.733 ± 0.041
2.305PhePhe: 2.305 ± 0.037
3.361PheGly: 3.361 ± 0.046
0.719PheHis: 0.719 ± 0.023
3.405PheIle: 3.405 ± 0.047
3.51PheLys: 3.51 ± 0.05
4.225PheLeu: 4.225 ± 0.049
1.12PheMet: 1.12 ± 0.023
3.33PheAsn: 3.33 ± 0.052
1.739PhePro: 1.739 ± 0.033
1.362PheGln: 1.362 ± 0.028
1.738PheArg: 1.738 ± 0.029
3.927PheSer: 3.927 ± 0.053
3.052PheThr: 3.052 ± 0.045
2.788PheVal: 2.788 ± 0.035
0.596PheTrp: 0.596 ± 0.018
2.148PheTyr: 2.148 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
4.792GlyAla: 4.792 ± 0.08
0.616GlyCys: 0.616 ± 0.022
3.47GlyAsp: 3.47 ± 0.046
3.327GlyGlu: 3.327 ± 0.047
3.621GlyPhe: 3.621 ± 0.042
5.147GlyGly: 5.147 ± 0.118
1.123GlyHis: 1.123 ± 0.024
5.388GlyIle: 5.388 ± 0.057
5.591GlyLys: 5.591 ± 0.064
6.497GlyLeu: 6.497 ± 0.068
1.695GlyMet: 1.695 ± 0.031
4.047GlyAsn: 4.047 ± 0.063
1.691GlyPro: 1.691 ± 0.03
2.324GlyGln: 2.324 ± 0.036
2.52GlyArg: 2.52 ± 0.038
4.755GlySer: 4.755 ± 0.057
4.614GlyThr: 4.614 ± 0.083
4.67GlyVal: 4.67 ± 0.062
0.939GlyTrp: 0.939 ± 0.023
3.27GlyTyr: 3.27 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
1.096HisAla: 1.096 ± 0.026
0.191HisCys: 0.191 ± 0.01
0.848HisAsp: 0.848 ± 0.021
0.897HisGlu: 0.897 ± 0.023
1.03HisPhe: 1.03 ± 0.025
1.072HisGly: 1.072 ± 0.028
0.443HisHis: 0.443 ± 0.016
1.247HisIle: 1.247 ± 0.028
1.031HisLys: 1.031 ± 0.024
1.719HisLeu: 1.719 ± 0.036
0.357HisMet: 0.357 ± 0.015
0.927HisAsn: 0.927 ± 0.022
0.884HisPro: 0.884 ± 0.025
0.692HisGln: 0.692 ± 0.021
0.653HisArg: 0.653 ± 0.019
0.935HisSer: 0.935 ± 0.023
0.933HisThr: 0.933 ± 0.023
0.913HisVal: 0.913 ± 0.022
0.204HisTrp: 0.204 ± 0.01
0.784HisTyr: 0.784 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.784IleAla: 5.784 ± 0.069
0.662IleCys: 0.662 ± 0.019
4.224IleAsp: 4.224 ± 0.049
4.169IleGlu: 4.169 ± 0.055
3.022IlePhe: 3.022 ± 0.048
4.869IleGly: 4.869 ± 0.056
1.21IleHis: 1.21 ± 0.028
4.803IleIle: 4.803 ± 0.072
5.238IleLys: 5.238 ± 0.065
6.193IleLeu: 6.193 ± 0.071
1.392IleMet: 1.392 ± 0.032
4.402IleAsn: 4.402 ± 0.05
3.078IlePro: 3.078 ± 0.044
2.249IleGln: 2.249 ± 0.038
2.849IleArg: 2.849 ± 0.042
5.351IleSer: 5.351 ± 0.058
4.593IleThr: 4.593 ± 0.056
4.243IleVal: 4.243 ± 0.059
0.737IleTrp: 0.737 ± 0.02
2.672IleTyr: 2.672 ± 0.042
0.0IleXaa: 0.0 ± 0.0
Lys
5.429LysAla: 5.429 ± 0.065
0.31LysCys: 0.31 ± 0.014
4.435LysAsp: 4.435 ± 0.047
4.836LysGlu: 4.836 ± 0.064
2.701LysPhe: 2.701 ± 0.041
5.016LysGly: 5.016 ± 0.056
1.318LysHis: 1.318 ± 0.029
5.216LysIle: 5.216 ± 0.055
5.543LysLys: 5.543 ± 0.08
6.427LysLeu: 6.427 ± 0.072
1.975LysMet: 1.975 ± 0.032
4.523LysAsn: 4.523 ± 0.054
2.877LysPro: 2.877 ± 0.046
2.766LysGln: 2.766 ± 0.045
2.868LysArg: 2.868 ± 0.044
4.264LysSer: 4.264 ± 0.057
4.305LysThr: 4.305 ± 0.055
4.715LysVal: 4.715 ± 0.053
0.872LysTrp: 0.872 ± 0.025
3.009LysTyr: 3.009 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
6.6LeuAla: 6.6 ± 0.063
0.758LeuCys: 0.758 ± 0.021
4.661LeuAsp: 4.661 ± 0.05
4.784LeuGlu: 4.784 ± 0.062
4.613LeuPhe: 4.613 ± 0.059
5.766LeuGly: 5.766 ± 0.063
1.49LeuHis: 1.49 ± 0.027
6.535LeuIle: 6.535 ± 0.081
7.768LeuLys: 7.768 ± 0.074
9.041LeuLeu: 9.041 ± 0.094
2.279LeuMet: 2.279 ± 0.035
6.215LeuAsn: 6.215 ± 0.076
3.989LeuPro: 3.989 ± 0.048
3.337LeuGln: 3.337 ± 0.049
3.551LeuArg: 3.551 ± 0.043
7.203LeuSer: 7.203 ± 0.07
5.658LeuThr: 5.658 ± 0.06
5.293LeuVal: 5.293 ± 0.051
0.971LeuTrp: 0.971 ± 0.024
3.334LeuTyr: 3.334 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
1.766MetAla: 1.766 ± 0.037
0.132MetCys: 0.132 ± 0.008
1.249MetAsp: 1.249 ± 0.03
1.355MetGlu: 1.355 ± 0.025
0.836MetPhe: 0.836 ± 0.019
1.57MetGly: 1.57 ± 0.031
0.375MetHis: 0.375 ± 0.014
1.52MetIle: 1.52 ± 0.034
2.034MetLys: 2.034 ± 0.037
2.163MetLeu: 2.163 ± 0.038
0.655MetMet: 0.655 ± 0.02
1.382MetAsn: 1.382 ± 0.028
1.095MetPro: 1.095 ± 0.027
0.925MetGln: 0.925 ± 0.021
0.978MetArg: 0.978 ± 0.018
1.423MetSer: 1.423 ± 0.025
1.106MetThr: 1.106 ± 0.026
1.455MetVal: 1.455 ± 0.033
0.217MetTrp: 0.217 ± 0.013
0.75MetTyr: 0.75 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
4.35AsnAla: 4.35 ± 0.057
0.403AsnCys: 0.403 ± 0.017
2.94AsnAsp: 2.94 ± 0.041
3.154AsnGlu: 3.154 ± 0.041
2.812AsnPhe: 2.812 ± 0.039
4.521AsnGly: 4.521 ± 0.054
0.987AsnHis: 0.987 ± 0.03
4.328AsnIle: 4.328 ± 0.053
4.165AsnLys: 4.165 ± 0.051
5.331AsnLeu: 5.331 ± 0.065
1.311AsnMet: 1.311 ± 0.028
3.513AsnAsn: 3.513 ± 0.067
2.862AsnPro: 2.862 ± 0.043
2.122AsnGln: 2.122 ± 0.033
2.375AsnArg: 2.375 ± 0.039
3.654AsnSer: 3.654 ± 0.055
3.672AsnThr: 3.672 ± 0.057
3.533AsnVal: 3.533 ± 0.058
0.81AsnTrp: 0.81 ± 0.02
2.818AsnTyr: 2.818 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
3.06ProAla: 3.06 ± 0.048
0.253ProCys: 0.253 ± 0.013
2.48ProAsp: 2.48 ± 0.04
2.85ProGlu: 2.85 ± 0.04
1.938ProPhe: 1.938 ± 0.034
2.944ProGly: 2.944 ± 0.049
0.602ProHis: 0.602 ± 0.021
2.428ProIle: 2.428 ± 0.04
2.27ProLys: 2.27 ± 0.036
3.346ProLeu: 3.346 ± 0.047
0.783ProMet: 0.783 ± 0.022
2.053ProAsn: 2.053 ± 0.037
0.959ProPro: 0.959 ± 0.027
1.257ProGln: 1.257 ± 0.027
1.133ProArg: 1.133 ± 0.027
2.319ProSer: 2.319 ± 0.039
2.029ProThr: 2.029 ± 0.042
3.06ProVal: 3.06 ± 0.045
0.387ProTrp: 0.387 ± 0.016
1.558ProTyr: 1.558 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
2.425GlnAla: 2.425 ± 0.035
0.182GlnCys: 0.182 ± 0.01
1.617GlnAsp: 1.617 ± 0.027
1.943GlnGlu: 1.943 ± 0.036
1.583GlnPhe: 1.583 ± 0.029
2.036GlnGly: 2.036 ± 0.031
0.695GlnHis: 0.695 ± 0.019
2.518GlnIle: 2.518 ± 0.038
2.629GlnLys: 2.629 ± 0.038
3.738GlnLeu: 3.738 ± 0.048
0.859GlnMet: 0.859 ± 0.024
2.106GlnAsn: 2.106 ± 0.035
1.322GlnPro: 1.322 ± 0.025
1.799GlnGln: 1.799 ± 0.037
1.44GlnArg: 1.44 ± 0.03
2.218GlnSer: 2.218 ± 0.034
2.12GlnThr: 2.12 ± 0.035
2.267GlnVal: 2.267 ± 0.039
0.396GlnTrp: 0.396 ± 0.018
1.513GlnTyr: 1.513 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
2.483ArgAla: 2.483 ± 0.044
0.223ArgCys: 0.223 ± 0.012
1.863ArgAsp: 1.863 ± 0.029
2.048ArgGlu: 2.048 ± 0.037
2.111ArgPhe: 2.111 ± 0.033
2.26ArgGly: 2.26 ± 0.037
0.591ArgHis: 0.591 ± 0.017
2.981ArgIle: 2.981 ± 0.041
2.875ArgLys: 2.875 ± 0.044
3.743ArgLeu: 3.743 ± 0.044
1.027ArgMet: 1.027 ± 0.026
2.388ArgAsn: 2.388 ± 0.031
1.329ArgPro: 1.329 ± 0.031
1.218ArgGln: 1.218 ± 0.03
1.471ArgArg: 1.471 ± 0.03
2.287ArgSer: 2.287 ± 0.036
2.064ArgThr: 2.064 ± 0.032
2.333ArgVal: 2.333 ± 0.039
0.577ArgTrp: 0.577 ± 0.016
1.835ArgTyr: 1.835 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
4.876SerAla: 4.876 ± 0.06
0.571SerCys: 0.571 ± 0.017
3.177SerAsp: 3.177 ± 0.043
3.03SerGlu: 3.03 ± 0.039
3.702SerPhe: 3.702 ± 0.046
5.303SerGly: 5.303 ± 0.067
0.992SerHis: 0.992 ± 0.026
4.907SerIle: 4.907 ± 0.06
4.268SerLys: 4.268 ± 0.051
6.244SerLeu: 6.244 ± 0.063
1.319SerMet: 1.319 ± 0.032
3.622SerAsn: 3.622 ± 0.051
2.432SerPro: 2.432 ± 0.039
1.901SerGln: 1.901 ± 0.033
2.536SerArg: 2.536 ± 0.041
4.408SerSer: 4.408 ± 0.058
3.775SerThr: 3.775 ± 0.061
4.394SerVal: 4.394 ± 0.049
0.837SerTrp: 0.837 ± 0.021
2.976SerTyr: 2.976 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.824ThrAla: 4.824 ± 0.076
0.324ThrCys: 0.324 ± 0.014
3.417ThrAsp: 3.417 ± 0.039
3.171ThrGlu: 3.171 ± 0.044
2.794ThrPhe: 2.794 ± 0.05
4.973ThrGly: 4.973 ± 0.075
0.918ThrHis: 0.918 ± 0.025
4.395ThrIle: 4.395 ± 0.063
3.56ThrLys: 3.56 ± 0.049
5.484ThrLeu: 5.484 ± 0.074
1.098ThrMet: 1.098 ± 0.024
3.199ThrAsn: 3.199 ± 0.056
2.56ThrPro: 2.56 ± 0.047
1.84ThrGln: 1.84 ± 0.031
1.862ThrArg: 1.862 ± 0.033
3.602ThrSer: 3.602 ± 0.056
3.659ThrThr: 3.659 ± 0.075
3.923ThrVal: 3.923 ± 0.064
0.715ThrTrp: 0.715 ± 0.018
2.55ThrTyr: 2.55 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
4.559ValAla: 4.559 ± 0.054
0.534ValCys: 0.534 ± 0.018
3.329ValAsp: 3.329 ± 0.044
3.281ValGlu: 3.281 ± 0.045
3.157ValPhe: 3.157 ± 0.036
3.768ValGly: 3.768 ± 0.055
0.962ValHis: 0.962 ± 0.022
4.662ValIle: 4.662 ± 0.058
4.921ValLys: 4.921 ± 0.05
6.08ValLeu: 6.08 ± 0.057
1.493ValMet: 1.493 ± 0.029
3.961ValAsn: 3.961 ± 0.052
2.466ValPro: 2.466 ± 0.036
1.996ValGln: 1.996 ± 0.034
2.246ValArg: 2.246 ± 0.038
4.63ValSer: 4.63 ± 0.047
3.876ValThr: 3.876 ± 0.069
4.362ValVal: 4.362 ± 0.06
0.7ValTrp: 0.7 ± 0.019
2.507ValTyr: 2.507 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
0.817TrpAla: 0.817 ± 0.022
0.142TrpCys: 0.142 ± 0.009
0.657TrpAsp: 0.657 ± 0.02
0.685TrpGlu: 0.685 ± 0.02
0.555TrpPhe: 0.555 ± 0.018
0.849TrpGly: 0.849 ± 0.024
0.239TrpHis: 0.239 ± 0.012
0.739TrpIle: 0.739 ± 0.021
0.932TrpLys: 0.932 ± 0.024
1.176TrpLeu: 1.176 ± 0.029
0.346TrpMet: 0.346 ± 0.015
0.71TrpAsn: 0.71 ± 0.018
0.36TrpPro: 0.36 ± 0.016
0.511TrpGln: 0.511 ± 0.016
0.492TrpArg: 0.492 ± 0.019
0.708TrpSer: 0.708 ± 0.021
0.7TrpThr: 0.7 ± 0.019
0.69TrpVal: 0.69 ± 0.022
0.187TrpTrp: 0.187 ± 0.011
0.543TrpTyr: 0.543 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.012TyrAla: 3.012 ± 0.041
0.317TyrCys: 0.317 ± 0.014
2.262TyrAsp: 2.262 ± 0.035
1.995TyrGlu: 1.995 ± 0.033
2.269TyrPhe: 2.269 ± 0.042
2.971TyrGly: 2.971 ± 0.046
0.834TyrHis: 0.834 ± 0.02
2.506TyrIle: 2.506 ± 0.038
2.933TyrLys: 2.933 ± 0.043
3.931TyrLeu: 3.931 ± 0.048
0.79TyrMet: 0.79 ± 0.021
2.769TyrAsn: 2.769 ± 0.044
1.722TyrPro: 1.722 ± 0.032
1.682TyrGln: 1.682 ± 0.031
1.839TyrArg: 1.839 ± 0.032
2.803TyrSer: 2.803 ± 0.046
2.665TyrThr: 2.665 ± 0.047
2.263TyrVal: 2.263 ± 0.037
0.545TyrTrp: 0.545 ± 0.019
1.878TyrTyr: 1.878 ± 0.034
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4961 proteins (1862186 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski