Achromobacter phage vB_Ade_ART

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5H2Q974|A0A5H2Q974_9CAUD Uncharacterized protein OS=Achromobacter phage vB_Ade_ART OX=2292880 GN=ART_00103 PE=4 SV=1
MM1 pKa = 7.77DD2 pKa = 3.5TQIRR6 pKa = 11.84PISLEE11 pKa = 3.4GWTKK15 pKa = 10.3VDD17 pKa = 3.06GVQPVRR23 pKa = 11.84DD24 pKa = 3.72SEE26 pKa = 4.6LIEE29 pKa = 4.09VLYY32 pKa = 11.22GDD34 pKa = 4.64VNPDD38 pKa = 3.33GYY40 pKa = 10.9RR41 pKa = 11.84VGIVHH46 pKa = 5.94TVVYY50 pKa = 10.59ADD52 pKa = 3.54EE53 pKa = 4.42VDD55 pKa = 2.83WAGRR59 pKa = 11.84VIQSPSFWRR68 pKa = 11.84YY69 pKa = 8.33FGDD72 pKa = 3.96YY73 pKa = 10.21IVV75 pKa = 4.02

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5H2Q7D3|A0A5H2Q7D3_9CAUD Uncharacterized protein OS=Achromobacter phage vB_Ade_ART OX=2292880 GN=ART_00104 PE=4 SV=1
MM1 pKa = 7.5LKK3 pKa = 10.5SLMVQPRR10 pKa = 11.84QPSAWAEE17 pKa = 3.75HH18 pKa = 5.63NKK20 pKa = 9.6RR21 pKa = 11.84VKK23 pKa = 10.75ANEE26 pKa = 4.63AIHH29 pKa = 6.73KK30 pKa = 8.99EE31 pKa = 4.0LEE33 pKa = 3.76ATFPHH38 pKa = 7.32KK39 pKa = 10.12FRR41 pKa = 11.84HH42 pKa = 5.26PRR44 pKa = 11.84IGRR47 pKa = 11.84QWMATRR53 pKa = 11.84DD54 pKa = 3.29WRR56 pKa = 11.84KK57 pKa = 9.95EE58 pKa = 3.34ITPAITGHH66 pKa = 6.23RR67 pKa = 11.84RR68 pKa = 11.84QSEE71 pKa = 4.2PPITWQILLGVLIVVTFLGACYY93 pKa = 10.55ALL95 pKa = 4.23

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

127

0

127

29721

44

1165

234.0

25.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.461 ± 0.261

0.895 ± 0.08

5.676 ± 0.174

6.531 ± 0.242

3.553 ± 0.142

8.031 ± 0.236

1.649 ± 0.135

5.289 ± 0.156

5.245 ± 0.263

8.031 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.826 ± 0.103

4.108 ± 0.168

5.064 ± 0.202

4.078 ± 0.128

5.774 ± 0.152

5.797 ± 0.181

5.844 ± 0.238

6.965 ± 0.166

1.871 ± 0.124

3.311 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski