Fervidicella metallireducens AeB

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Fervidicella; Fervidicella metallireducens

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2800 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A017RXM9|A0A017RXM9_9CLOT Phosphoribosylaminoimidazole-succinocarboxamide synthase OS=Fervidicella metallireducens AeB OX=1403537 GN=purC PE=3 SV=1
MM1 pKa = 7.13NSCMIFWVPIYY12 pKa = 9.37PQNSKK17 pKa = 10.42NLNIYY22 pKa = 10.8RR23 pKa = 11.84NDD25 pKa = 3.14TTLAEE30 pKa = 4.3EE31 pKa = 4.05EE32 pKa = 4.69TEE34 pKa = 4.28NSLEE38 pKa = 3.98MLFDD42 pKa = 5.2EE43 pKa = 5.19IDD45 pKa = 3.38EE46 pKa = 4.67EE47 pKa = 4.97YY48 pKa = 10.35ISQSLGQQ55 pKa = 3.57

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A017RXL7|A0A017RXL7_9CLOT Flavodoxin OS=Fervidicella metallireducens AeB OX=1403537 GN=Q428_04130 PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.46KK9 pKa = 8.28RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.69RR21 pKa = 11.84MKK23 pKa = 8.73TRR25 pKa = 11.84GGRR28 pKa = 11.84LVLQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.92GRR39 pKa = 11.84KK40 pKa = 9.02RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2800

0

2800

781929

29

2410

279.3

31.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.729 ± 0.051

1.2 ± 0.021

5.418 ± 0.035

7.436 ± 0.052

4.395 ± 0.036

6.455 ± 0.046

1.384 ± 0.018

9.993 ± 0.053

9.002 ± 0.048

8.987 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.023

5.915 ± 0.047

2.945 ± 0.028

2.428 ± 0.02

3.822 ± 0.032

5.929 ± 0.037

4.865 ± 0.04

6.593 ± 0.043

0.668 ± 0.012

4.195 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski