Amino acid dipepetide frequency for Shigella phage Sf12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.59AlaAla: 7.59 ± 0.993
0.633AlaCys: 0.633 ± 0.257
4.076AlaAsp: 4.076 ± 0.715
5.411AlaGlu: 5.411 ± 1.093
2.319AlaPhe: 2.319 ± 0.551
7.59AlaGly: 7.59 ± 1.306
1.124AlaHis: 1.124 ± 0.282
6.395AlaIle: 6.395 ± 0.692
5.903AlaLys: 5.903 ± 0.941
7.028AlaLeu: 7.028 ± 0.757
2.881AlaMet: 2.881 ± 0.554
4.076AlaAsn: 4.076 ± 0.766
1.546AlaPro: 1.546 ± 0.391
3.303AlaGln: 3.303 ± 0.573
4.498AlaArg: 4.498 ± 0.575
5.201AlaSer: 5.201 ± 0.665
5.201AlaThr: 5.201 ± 0.72
5.201AlaVal: 5.201 ± 0.631
1.265AlaTrp: 1.265 ± 0.378
2.389AlaTyr: 2.389 ± 0.501
0.0AlaXaa: 0.0 ± 0.0
Cys
0.492CysAla: 0.492 ± 0.227
0.07CysCys: 0.07 ± 0.063
0.773CysAsp: 0.773 ± 0.213
0.914CysGlu: 0.914 ± 0.275
0.633CysPhe: 0.633 ± 0.232
1.054CysGly: 1.054 ± 0.338
0.211CysHis: 0.211 ± 0.116
0.843CysIle: 0.843 ± 0.274
0.843CysLys: 0.843 ± 0.263
0.843CysLeu: 0.843 ± 0.276
0.351CysMet: 0.351 ± 0.175
0.422CysAsn: 0.422 ± 0.213
0.211CysPro: 0.211 ± 0.105
0.281CysGln: 0.281 ± 0.14
0.703CysArg: 0.703 ± 0.291
0.703CysSer: 0.703 ± 0.236
0.562CysThr: 0.562 ± 0.189
0.703CysVal: 0.703 ± 0.233
0.141CysTrp: 0.141 ± 0.099
0.422CysTyr: 0.422 ± 0.161
0.0CysXaa: 0.0 ± 0.0
Asp
4.849AspAla: 4.849 ± 0.669
0.633AspCys: 0.633 ± 0.202
4.357AspAsp: 4.357 ± 0.696
4.568AspGlu: 4.568 ± 0.453
2.389AspPhe: 2.389 ± 0.454
6.325AspGly: 6.325 ± 0.925
1.124AspHis: 1.124 ± 0.297
3.584AspIle: 3.584 ± 0.42
4.779AspLys: 4.779 ± 0.575
3.655AspLeu: 3.655 ± 0.615
1.616AspMet: 1.616 ± 0.377
3.092AspAsn: 3.092 ± 0.509
1.406AspPro: 1.406 ± 0.247
1.476AspGln: 1.476 ± 0.392
2.108AspArg: 2.108 ± 0.523
3.725AspSer: 3.725 ± 0.554
3.163AspThr: 3.163 ± 0.51
3.584AspVal: 3.584 ± 0.416
1.054AspTrp: 1.054 ± 0.283
3.022AspTyr: 3.022 ± 0.511
0.0AspXaa: 0.0 ± 0.0
Glu
5.693GluAla: 5.693 ± 0.671
0.914GluCys: 0.914 ± 0.259
3.514GluAsp: 3.514 ± 0.549
4.006GluGlu: 4.006 ± 0.66
3.584GluPhe: 3.584 ± 0.484
4.287GluGly: 4.287 ± 0.526
0.773GluHis: 0.773 ± 0.229
6.255GluIle: 6.255 ± 0.574
3.444GluLys: 3.444 ± 0.651
5.693GluLeu: 5.693 ± 0.649
2.038GluMet: 2.038 ± 0.517
3.795GluAsn: 3.795 ± 0.535
1.195GluPro: 1.195 ± 0.329
2.811GluGln: 2.811 ± 0.456
3.233GluArg: 3.233 ± 0.532
4.076GluSer: 4.076 ± 0.722
3.514GluThr: 3.514 ± 0.458
4.99GluVal: 4.99 ± 0.77
0.562GluTrp: 0.562 ± 0.222
1.898GluTyr: 1.898 ± 0.402
0.0GluXaa: 0.0 ± 0.0
Phe
2.952PheAla: 2.952 ± 0.585
0.703PheCys: 0.703 ± 0.221
3.655PheAsp: 3.655 ± 0.495
2.53PheGlu: 2.53 ± 0.466
0.703PhePhe: 0.703 ± 0.28
3.514PheGly: 3.514 ± 0.469
0.422PheHis: 0.422 ± 0.186
2.249PheIle: 2.249 ± 0.328
2.179PheLys: 2.179 ± 0.395
2.249PheLeu: 2.249 ± 0.379
0.703PheMet: 0.703 ± 0.218
2.53PheAsn: 2.53 ± 0.43
1.546PhePro: 1.546 ± 0.333
1.476PheGln: 1.476 ± 0.358
2.038PheArg: 2.038 ± 0.428
2.389PheSer: 2.389 ± 0.446
2.671PheThr: 2.671 ± 0.486
1.968PheVal: 1.968 ± 0.354
0.422PheTrp: 0.422 ± 0.151
1.124PheTyr: 1.124 ± 0.274
0.0PheXaa: 0.0 ± 0.0
Gly
4.92GlyAla: 4.92 ± 0.735
0.984GlyCys: 0.984 ± 0.282
4.568GlyAsp: 4.568 ± 0.765
5.693GlyGlu: 5.693 ± 0.933
2.741GlyPhe: 2.741 ± 0.54
6.185GlyGly: 6.185 ± 1.166
0.843GlyHis: 0.843 ± 0.324
4.217GlyIle: 4.217 ± 0.601
5.06GlyLys: 5.06 ± 0.56
6.958GlyLeu: 6.958 ± 0.646
2.881GlyMet: 2.881 ± 0.481
3.655GlyAsn: 3.655 ± 0.52
3.022GlyPro: 3.022 ± 2.32
1.687GlyGln: 1.687 ± 0.393
3.373GlyArg: 3.373 ± 0.485
6.114GlySer: 6.114 ± 0.716
4.568GlyThr: 4.568 ± 0.695
7.731GlyVal: 7.731 ± 0.895
0.914GlyTrp: 0.914 ± 0.231
4.146GlyTyr: 4.146 ± 0.529
0.0GlyXaa: 0.0 ± 0.0
His
0.984HisAla: 0.984 ± 0.287
0.211HisCys: 0.211 ± 0.118
0.703HisAsp: 0.703 ± 0.245
1.054HisGlu: 1.054 ± 0.249
0.703HisPhe: 0.703 ± 0.238
1.335HisGly: 1.335 ± 0.376
0.562HisHis: 0.562 ± 0.234
0.984HisIle: 0.984 ± 0.321
1.476HisLys: 1.476 ± 0.423
1.335HisLeu: 1.335 ± 0.345
0.141HisMet: 0.141 ± 0.098
0.633HisAsn: 0.633 ± 0.245
0.351HisPro: 0.351 ± 0.154
0.351HisGln: 0.351 ± 0.176
1.335HisArg: 1.335 ± 0.33
0.773HisSer: 0.773 ± 0.225
0.703HisThr: 0.703 ± 0.214
0.843HisVal: 0.843 ± 0.282
0.0HisTrp: 0.0 ± 0.0
0.703HisTyr: 0.703 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
5.763IleAla: 5.763 ± 0.94
0.843IleCys: 0.843 ± 0.249
5.763IleAsp: 5.763 ± 0.714
4.568IleGlu: 4.568 ± 0.655
1.898IlePhe: 1.898 ± 0.353
4.357IleGly: 4.357 ± 0.545
0.703IleHis: 0.703 ± 0.218
3.163IleIle: 3.163 ± 0.462
4.568IleLys: 4.568 ± 0.588
2.811IleLeu: 2.811 ± 0.338
0.984IleMet: 0.984 ± 0.294
4.006IleAsn: 4.006 ± 0.604
2.671IlePro: 2.671 ± 0.462
2.319IleGln: 2.319 ± 0.409
3.303IleArg: 3.303 ± 0.513
3.373IleSer: 3.373 ± 0.457
4.357IleThr: 4.357 ± 0.569
3.514IleVal: 3.514 ± 0.507
0.914IleTrp: 0.914 ± 0.24
2.6IleTyr: 2.6 ± 0.429
0.0IleXaa: 0.0 ± 0.0
Lys
6.606LysAla: 6.606 ± 0.888
0.351LysCys: 0.351 ± 0.165
3.725LysAsp: 3.725 ± 0.497
3.655LysGlu: 3.655 ± 0.614
2.038LysPhe: 2.038 ± 0.385
3.163LysGly: 3.163 ± 0.56
1.265LysHis: 1.265 ± 0.33
3.725LysIle: 3.725 ± 0.529
3.936LysLys: 3.936 ± 0.683
6.044LysLeu: 6.044 ± 0.675
3.444LysMet: 3.444 ± 0.671
2.952LysAsn: 2.952 ± 0.514
1.898LysPro: 1.898 ± 0.432
3.092LysGln: 3.092 ± 0.532
2.6LysArg: 2.6 ± 0.444
4.498LysSer: 4.498 ± 0.64
3.865LysThr: 3.865 ± 0.565
4.92LysVal: 4.92 ± 0.667
0.633LysTrp: 0.633 ± 0.222
2.389LysTyr: 2.389 ± 0.409
0.0LysXaa: 0.0 ± 0.0
Leu
6.817LeuAla: 6.817 ± 0.592
0.773LeuCys: 0.773 ± 0.215
4.076LeuAsp: 4.076 ± 0.571
4.568LeuGlu: 4.568 ± 0.466
1.898LeuPhe: 1.898 ± 0.291
4.849LeuGly: 4.849 ± 0.87
1.265LeuHis: 1.265 ± 0.35
3.865LeuIle: 3.865 ± 0.468
4.217LeuLys: 4.217 ± 0.475
4.568LeuLeu: 4.568 ± 0.642
1.546LeuMet: 1.546 ± 0.317
3.514LeuAsn: 3.514 ± 0.456
2.952LeuPro: 2.952 ± 0.488
2.249LeuGln: 2.249 ± 0.442
4.568LeuArg: 4.568 ± 0.63
5.763LeuSer: 5.763 ± 0.569
5.482LeuThr: 5.482 ± 0.543
5.411LeuVal: 5.411 ± 0.566
0.562LeuTrp: 0.562 ± 0.184
2.389LeuTyr: 2.389 ± 0.368
0.0LeuXaa: 0.0 ± 0.0
Met
3.303MetAla: 3.303 ± 0.344
0.141MetCys: 0.141 ± 0.095
0.773MetAsp: 0.773 ± 0.269
1.406MetGlu: 1.406 ± 0.361
0.843MetPhe: 0.843 ± 0.276
1.054MetGly: 1.054 ± 0.286
0.703MetHis: 0.703 ± 0.231
1.827MetIle: 1.827 ± 0.318
2.319MetLys: 2.319 ± 0.408
1.616MetLeu: 1.616 ± 0.407
1.124MetMet: 1.124 ± 0.394
1.616MetAsn: 1.616 ± 0.419
0.492MetPro: 0.492 ± 0.198
0.843MetGln: 0.843 ± 0.245
1.968MetArg: 1.968 ± 0.398
1.757MetSer: 1.757 ± 0.263
1.968MetThr: 1.968 ± 0.515
1.476MetVal: 1.476 ± 0.416
0.422MetTrp: 0.422 ± 0.158
0.492MetTyr: 0.492 ± 0.196
0.0MetXaa: 0.0 ± 0.0
Asn
4.287AsnAla: 4.287 ± 0.599
0.633AsnCys: 0.633 ± 0.206
2.952AsnAsp: 2.952 ± 0.408
3.655AsnGlu: 3.655 ± 0.462
1.195AsnPhe: 1.195 ± 0.332
5.903AsnGly: 5.903 ± 0.768
1.265AsnHis: 1.265 ± 0.372
2.811AsnIle: 2.811 ± 0.425
3.163AsnLys: 3.163 ± 0.452
3.936AsnLeu: 3.936 ± 0.467
0.703AsnMet: 0.703 ± 0.244
3.163AsnAsn: 3.163 ± 0.609
1.968AsnPro: 1.968 ± 0.314
2.038AsnGln: 2.038 ± 0.458
2.389AsnArg: 2.389 ± 0.452
3.795AsnSer: 3.795 ± 0.535
2.319AsnThr: 2.319 ± 0.478
3.514AsnVal: 3.514 ± 0.38
0.914AsnTrp: 0.914 ± 0.21
1.898AsnTyr: 1.898 ± 0.422
0.0AsnXaa: 0.0 ± 0.0
Pro
3.303ProAla: 3.303 ± 0.683
0.422ProCys: 0.422 ± 0.289
1.898ProAsp: 1.898 ± 0.377
2.811ProGlu: 2.811 ± 0.534
2.038ProPhe: 2.038 ± 0.381
2.108ProGly: 2.108 ± 0.458
0.351ProHis: 0.351 ± 0.147
1.616ProIle: 1.616 ± 0.381
1.054ProLys: 1.054 ± 0.249
2.179ProLeu: 2.179 ± 0.474
0.492ProMet: 0.492 ± 0.185
1.406ProAsn: 1.406 ± 0.315
0.843ProPro: 0.843 ± 0.24
2.249ProGln: 2.249 ± 1.007
1.476ProArg: 1.476 ± 0.252
1.687ProSer: 1.687 ± 0.33
1.476ProThr: 1.476 ± 0.402
3.163ProVal: 3.163 ± 0.483
0.211ProTrp: 0.211 ± 0.111
1.054ProTyr: 1.054 ± 0.242
0.0ProXaa: 0.0 ± 0.0
Gln
3.092GlnAla: 3.092 ± 0.676
0.422GlnCys: 0.422 ± 0.188
1.968GlnAsp: 1.968 ± 0.352
2.53GlnGlu: 2.53 ± 0.544
1.265GlnPhe: 1.265 ± 0.318
2.952GlnGly: 2.952 ± 1.475
0.281GlnHis: 0.281 ± 0.159
2.811GlnIle: 2.811 ± 0.547
2.741GlnLys: 2.741 ± 0.43
2.952GlnLeu: 2.952 ± 0.566
0.843GlnMet: 0.843 ± 0.316
1.406GlnAsn: 1.406 ± 0.357
1.476GlnPro: 1.476 ± 0.302
2.952GlnGln: 2.952 ± 0.944
1.898GlnArg: 1.898 ± 0.575
2.53GlnSer: 2.53 ± 0.385
1.968GlnThr: 1.968 ± 0.327
2.53GlnVal: 2.53 ± 0.451
0.562GlnTrp: 0.562 ± 0.218
1.476GlnTyr: 1.476 ± 0.346
0.0GlnXaa: 0.0 ± 0.0
Arg
3.303ArgAla: 3.303 ± 0.492
0.984ArgCys: 0.984 ± 0.37
2.038ArgAsp: 2.038 ± 0.331
3.584ArgGlu: 3.584 ± 0.572
2.811ArgPhe: 2.811 ± 0.338
3.725ArgGly: 3.725 ± 0.462
0.843ArgHis: 0.843 ± 0.258
2.881ArgIle: 2.881 ± 0.422
3.865ArgLys: 3.865 ± 0.573
3.936ArgLeu: 3.936 ± 0.641
0.914ArgMet: 0.914 ± 0.252
2.319ArgAsn: 2.319 ± 0.415
1.898ArgPro: 1.898 ± 0.355
2.319ArgGln: 2.319 ± 0.609
2.319ArgArg: 2.319 ± 0.374
2.46ArgSer: 2.46 ± 0.368
2.389ArgThr: 2.389 ± 0.439
3.795ArgVal: 3.795 ± 0.539
0.562ArgTrp: 0.562 ± 0.256
1.757ArgTyr: 1.757 ± 0.352
0.0ArgXaa: 0.0 ± 0.0
Ser
5.903SerAla: 5.903 ± 0.848
0.422SerCys: 0.422 ± 0.205
4.709SerAsp: 4.709 ± 0.585
4.287SerGlu: 4.287 ± 0.562
2.952SerPhe: 2.952 ± 0.411
6.606SerGly: 6.606 ± 0.724
0.914SerHis: 0.914 ± 0.287
3.584SerIle: 3.584 ± 0.47
4.006SerLys: 4.006 ± 0.671
4.99SerLeu: 4.99 ± 0.521
1.335SerMet: 1.335 ± 0.288
3.373SerAsn: 3.373 ± 0.373
1.898SerPro: 1.898 ± 0.404
2.741SerGln: 2.741 ± 0.471
2.319SerArg: 2.319 ± 0.369
3.865SerSer: 3.865 ± 0.494
3.444SerThr: 3.444 ± 0.541
4.99SerVal: 4.99 ± 0.577
1.054SerTrp: 1.054 ± 0.321
3.444SerTyr: 3.444 ± 0.475
0.0SerXaa: 0.0 ± 0.0
Thr
5.622ThrAla: 5.622 ± 0.903
0.914ThrCys: 0.914 ± 0.207
2.6ThrAsp: 2.6 ± 0.438
3.303ThrGlu: 3.303 ± 0.416
2.249ThrPhe: 2.249 ± 0.385
6.325ThrGly: 6.325 ± 0.904
0.703ThrHis: 0.703 ± 0.227
3.936ThrIle: 3.936 ± 0.457
2.741ThrLys: 2.741 ± 0.512
3.865ThrLeu: 3.865 ± 0.504
1.335ThrMet: 1.335 ± 0.34
3.514ThrAsn: 3.514 ± 0.561
2.741ThrPro: 2.741 ± 0.444
1.827ThrGln: 1.827 ± 0.393
2.319ThrArg: 2.319 ± 0.343
4.357ThrSer: 4.357 ± 0.621
3.936ThrThr: 3.936 ± 0.668
4.709ThrVal: 4.709 ± 0.681
0.633ThrTrp: 0.633 ± 0.227
2.53ThrTyr: 2.53 ± 0.418
0.0ThrXaa: 0.0 ± 0.0
Val
5.552ValAla: 5.552 ± 0.858
0.492ValCys: 0.492 ± 0.2
4.568ValAsp: 4.568 ± 0.462
4.357ValGlu: 4.357 ± 0.712
3.163ValPhe: 3.163 ± 0.435
5.13ValGly: 5.13 ± 0.668
0.843ValHis: 0.843 ± 0.211
4.849ValIle: 4.849 ± 0.613
5.693ValLys: 5.693 ± 0.879
3.584ValLeu: 3.584 ± 0.477
1.827ValMet: 1.827 ± 0.371
4.076ValAsn: 4.076 ± 0.543
2.179ValPro: 2.179 ± 0.446
2.881ValGln: 2.881 ± 0.49
3.303ValArg: 3.303 ± 0.499
6.325ValSer: 6.325 ± 0.573
4.217ValThr: 4.217 ± 0.557
5.201ValVal: 5.201 ± 0.682
0.843ValTrp: 0.843 ± 0.211
2.46ValTyr: 2.46 ± 0.405
0.0ValXaa: 0.0 ± 0.0
Trp
0.703TrpAla: 0.703 ± 0.213
0.141TrpCys: 0.141 ± 0.105
0.633TrpAsp: 0.633 ± 0.177
0.843TrpGlu: 0.843 ± 0.283
0.633TrpPhe: 0.633 ± 0.246
0.914TrpGly: 0.914 ± 0.203
0.281TrpHis: 0.281 ± 0.14
0.843TrpIle: 0.843 ± 0.218
0.562TrpLys: 0.562 ± 0.183
0.984TrpLeu: 0.984 ± 0.208
0.07TrpMet: 0.07 ± 0.078
0.843TrpAsn: 0.843 ± 0.3
0.422TrpPro: 0.422 ± 0.172
0.211TrpGln: 0.211 ± 0.12
0.843TrpArg: 0.843 ± 0.254
0.843TrpSer: 0.843 ± 0.338
0.984TrpThr: 0.984 ± 0.263
1.054TrpVal: 1.054 ± 0.291
0.141TrpTrp: 0.141 ± 0.097
0.281TrpTyr: 0.281 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.898TyrAla: 1.898 ± 0.357
0.492TyrCys: 0.492 ± 0.269
3.163TyrAsp: 3.163 ± 0.598
2.53TyrGlu: 2.53 ± 0.493
1.968TyrPhe: 1.968 ± 0.425
2.881TyrGly: 2.881 ± 0.455
0.703TyrHis: 0.703 ± 0.239
2.038TyrIle: 2.038 ± 0.389
2.319TyrLys: 2.319 ± 0.412
2.108TyrLeu: 2.108 ± 0.377
0.843TyrMet: 0.843 ± 0.244
2.108TyrAsn: 2.108 ± 0.341
1.124TyrPro: 1.124 ± 0.296
1.546TyrGln: 1.546 ± 0.269
2.108TyrArg: 2.108 ± 0.42
2.6TyrSer: 2.6 ± 0.459
3.373TyrThr: 3.373 ± 0.537
2.249TyrVal: 2.249 ± 0.421
0.422TyrTrp: 0.422 ± 0.171
1.265TyrTyr: 1.265 ± 0.334
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (14230 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski