Strongyloides ratti (Parasitic roundworm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Tylenchina; Panagrolaimomorpha; Strongyloidoidea; Strongyloididae; Strongyloides

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090L4J0|A0A090L4J0_STRRB Thrombospondin type 1 repeat-containing protein OS=Strongyloides ratti OX=34506 GN=SRAE_1000068700 PE=4 SV=1
MM1 pKa = 7.82LYY3 pKa = 10.49NLSFDD8 pKa = 3.91VLSDD12 pKa = 3.21KK13 pKa = 10.54STSQEE18 pKa = 3.82NNVLLKK24 pKa = 10.93LPNNDD29 pKa = 2.25VTTQNIPTEE38 pKa = 4.4SEE40 pKa = 4.16EE41 pKa = 4.13EE42 pKa = 3.94MSDD45 pKa = 3.14PFVFTTSIPSVSPQPPGIDD64 pKa = 3.33LSFDD68 pKa = 3.63NNLEE72 pKa = 4.14TTTLDD77 pKa = 3.47SLIANSRR84 pKa = 11.84TTNFIEE90 pKa = 5.33LSTLKK95 pKa = 10.77DD96 pKa = 3.63DD97 pKa = 6.6FNLEE101 pKa = 4.27DD102 pKa = 4.36NDD104 pKa = 4.83TIEE107 pKa = 5.53DD108 pKa = 3.79DD109 pKa = 3.94QINITVAPLNSSKK122 pKa = 10.47QVSLSNDD129 pKa = 3.01FDD131 pKa = 4.23NKK133 pKa = 9.8TSEE136 pKa = 4.09GVNNFSNFDD145 pKa = 3.51INDD148 pKa = 3.42QYY150 pKa = 12.05VSLISKK156 pKa = 8.86EE157 pKa = 4.12AFITTTPANEE167 pKa = 4.41LNDD170 pKa = 3.73NDD172 pKa = 4.67EE173 pKa = 5.43LDD175 pKa = 3.99NLDD178 pKa = 4.45DD179 pKa = 5.75NEE181 pKa = 4.35IFLNTSSTTTILPKK195 pKa = 9.75MVNEE199 pKa = 4.82NIPLTTEE206 pKa = 4.25VYY208 pKa = 7.32PTNITSTSEE217 pKa = 3.86EE218 pKa = 3.91VHH220 pKa = 6.65LNNSDD225 pKa = 3.28KK226 pKa = 11.23FIVDD230 pKa = 4.28EE231 pKa = 4.3YY232 pKa = 11.03NGNSIEE238 pKa = 4.14NKK240 pKa = 7.45YY241 pKa = 9.38TNDD244 pKa = 3.08IKK246 pKa = 11.01KK247 pKa = 10.0LCPEE251 pKa = 4.21VEE253 pKa = 4.05ICNPGCGISIDD264 pKa = 3.78FDD266 pKa = 3.78GCQKK270 pKa = 9.05CTCLWIPNFCIEE282 pKa = 4.33TSDD285 pKa = 4.11CNGPEE290 pKa = 4.21YY291 pKa = 10.2ICEE294 pKa = 3.99YY295 pKa = 10.59GKK297 pKa = 10.4CLCDD301 pKa = 3.49INYY304 pKa = 7.56TQDD307 pKa = 3.13MEE309 pKa = 4.56RR310 pKa = 11.84SGICIPKK317 pKa = 9.99PNSLKK322 pKa = 10.96QNDD325 pKa = 3.5VV326 pKa = 3.14

Molecular weight: 36.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090LSW4|A0A090LSW4_STRRB Poly-glutamine tract binding protein 1 OS=Strongyloides ratti OX=34506 GN=SRAE_X000061900 PE=4 SV=1
MM1 pKa = 7.21FKK3 pKa = 10.42IMNLLNFNFFVIFFGIFIFTINGCQLKK30 pKa = 10.46YY31 pKa = 10.88SGIFGRR37 pKa = 11.84KK38 pKa = 6.69QCSLVINLNFDD49 pKa = 3.78PFFKK53 pKa = 10.43MSAFCLHH60 pKa = 6.18SAVYY64 pKa = 10.46QNLLRR69 pKa = 11.84MNKK72 pKa = 8.58QRR74 pKa = 11.84MGMNPKK80 pKa = 10.39LNAKK84 pKa = 10.04LLMDD88 pKa = 3.51MTMYY92 pKa = 11.2ANNNIGPKK100 pKa = 10.31NIMQLCTQCGTQCGMQTKK118 pKa = 9.8IKK120 pKa = 10.52FIGDD124 pKa = 4.49PIQGQGSLRR133 pKa = 11.84RR134 pKa = 11.84RR135 pKa = 11.84GSLTLSQRR143 pKa = 11.84RR144 pKa = 11.84KK145 pKa = 10.18NVFDD149 pKa = 3.89NAIHH153 pKa = 7.77DD154 pKa = 4.62DD155 pKa = 4.04ADD157 pKa = 3.77DD158 pKa = 5.06PIYY161 pKa = 10.78ATVNRR166 pKa = 11.84NRR168 pKa = 11.84FRR170 pKa = 11.84GSINRR175 pKa = 11.84GNSFNRR181 pKa = 11.84GGSFHH186 pKa = 7.31SNGGSFRR193 pKa = 11.84GGGGSFRR200 pKa = 11.84NGGGFNGRR208 pKa = 11.84GSLHH212 pKa = 6.73RR213 pKa = 11.84SNSVEE218 pKa = 3.57QRR220 pKa = 11.84RR221 pKa = 11.84RR222 pKa = 11.84AVFANEE228 pKa = 4.18RR229 pKa = 11.84LDD231 pKa = 3.6SDD233 pKa = 3.94EE234 pKa = 4.02

Molecular weight: 26.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9956

0

9956

4680237

14

12256

470.1

53.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.902 ± 0.022

2.063 ± 0.019

5.51 ± 0.02

6.64 ± 0.032

4.906 ± 0.026

4.755 ± 0.031

1.953 ± 0.009

8.57 ± 0.029

8.621 ± 0.031

8.628 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.011

7.619 ± 0.034

3.835 ± 0.026

3.372 ± 0.017

3.831 ± 0.025

8.016 ± 0.029

5.557 ± 0.039

5.051 ± 0.019

0.888 ± 0.008

4.063 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski