Caldibacillus debilis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Caldibacillus

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A150M7X3|A0A150M7X3_9BACI 30S ribosomal protein S4 OS=Caldibacillus debilis OX=301148 GN=rpsD PE=3 SV=1
MM1 pKa = 7.14VCVDD5 pKa = 4.47KK6 pKa = 11.44NQEE9 pKa = 4.02EE10 pKa = 4.82YY11 pKa = 11.59GMEE14 pKa = 4.11SWNKK18 pKa = 8.98TIYY21 pKa = 10.5KK22 pKa = 9.78EE23 pKa = 3.96IIEE26 pKa = 4.33PEE28 pKa = 4.05KK29 pKa = 10.43IVYY32 pKa = 9.39IDD34 pKa = 3.8YY35 pKa = 10.94FSDD38 pKa = 3.81SEE40 pKa = 4.77GNMSS44 pKa = 3.79

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A150L9Q0|A0A150L9Q0_9BACI Transketolase N-terminal section OS=Caldibacillus debilis OX=301148 GN=B4135_4149 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 10.42ASGGRR7 pKa = 11.84SRR9 pKa = 11.84VKK11 pKa = 10.05IRR13 pKa = 11.84PGNPAGATRR22 pKa = 11.84SARR25 pKa = 11.84RR26 pKa = 11.84APFSAFGGKK35 pKa = 9.54GRR37 pKa = 11.84ILLNLL42 pKa = 3.7

Molecular weight: 4.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4240

0

4240

910805

37

2865

214.8

24.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.636 ± 0.046

0.849 ± 0.012

4.737 ± 0.032

7.63 ± 0.057

5.071 ± 0.048

7.627 ± 0.042

1.981 ± 0.017

7.019 ± 0.041

7.142 ± 0.037

9.675 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.021

3.627 ± 0.03

4.53 ± 0.031

3.088 ± 0.025

6.046 ± 0.044

5.342 ± 0.035

4.562 ± 0.026

6.491 ± 0.033

1.059 ± 0.016

3.244 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski