Tilletia controversa (dwarf bunt fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Exobasidiomycetes; Tilletiales; Tilletiaceae; Tilletia

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9854 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177VU25|A0A177VU25_9BASI DUF155 domain-containing protein OS=Tilletia controversa OX=13291 GN=A4X06_g1366 PE=3 SV=1
MM1 pKa = 7.37LHH3 pKa = 6.79HH4 pKa = 6.93SLLAATILATTASYY18 pKa = 11.31ALPAQGDD25 pKa = 3.74MSFLSSTSEE34 pKa = 3.89LLFSRR39 pKa = 11.84TEE41 pKa = 3.93NGTSATEE48 pKa = 3.62ICADD52 pKa = 3.76GNQLVYY58 pKa = 10.39TPSTNGGPGSPQQIILSKK76 pKa = 10.41LYY78 pKa = 9.82QQAGVLTNVKK88 pKa = 9.64IDD90 pKa = 3.65FTEE93 pKa = 4.09ALAVSAGSVALNCQVASGTADD114 pKa = 3.33LTQDD118 pKa = 3.13QKK120 pKa = 11.81LSVILPIICAPVTAANPAPGIVGTGTGTGSAPAPADD156 pKa = 3.34PAAPAPAAPTDD167 pKa = 3.94PAAAPADD174 pKa = 4.23PNAAPADD181 pKa = 4.11PNAAPADD188 pKa = 4.11PNAAPADD195 pKa = 4.11PNAAPADD202 pKa = 3.86PATPGAAGDD211 pKa = 4.0AGTGTGTGTGTGDD224 pKa = 3.53GTDD227 pKa = 3.41KK228 pKa = 11.4GVGAGDD234 pKa = 3.81AGSGTGTGDD243 pKa = 3.23STGTGTGDD251 pKa = 3.15GTGAGSGTGAGGTGNGDD268 pKa = 3.44GTGTATGTGTGSGTGNGDD286 pKa = 3.32GTGTGAGGNGSGTGDD301 pKa = 3.63GGNGTPATGGDD312 pKa = 3.64GSNGVAGAAGNNGNGNANGTSNSGTGQRR340 pKa = 11.84AAGGSVGPITASTSMFTIVVAGAAYY365 pKa = 8.52TLLMLL370 pKa = 5.52

Molecular weight: 33.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177VLU4|A0A177VLU4_9BASI USP domain-containing protein OS=Tilletia controversa OX=13291 GN=A4X06_g4269 PE=4 SV=1
MM1 pKa = 7.39SVLNSRR7 pKa = 11.84ILATAARR14 pKa = 11.84ATASAVAARR23 pKa = 11.84APTALTTLARR33 pKa = 11.84RR34 pKa = 11.84QAASPSRR41 pKa = 11.84SLSTSRR47 pKa = 11.84TRR49 pKa = 11.84PNTRR53 pKa = 11.84CTSATPLIPTGSGSASFCSAAVRR76 pKa = 11.84SGASGSHH83 pKa = 6.27GGGKK87 pKa = 10.28LKK89 pKa = 9.21THH91 pKa = 6.87MGTKK95 pKa = 9.91KK96 pKa = 10.18RR97 pKa = 11.84FFPVGSIVGMFKK109 pKa = 10.4RR110 pKa = 11.84GRR112 pKa = 11.84AGKK115 pKa = 9.42SHH117 pKa = 7.01LNSHH121 pKa = 6.35MSSVRR126 pKa = 11.84RR127 pKa = 11.84SRR129 pKa = 11.84LRR131 pKa = 11.84GMAITPPGQTARR143 pKa = 11.84HH144 pKa = 6.29LKK146 pKa = 10.33RR147 pKa = 11.84LLGPILL153 pKa = 3.78

Molecular weight: 15.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9854

0

9854

5235128

9

5359

531.3

57.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.969 ± 0.028

0.942 ± 0.008

5.608 ± 0.019

5.691 ± 0.025

3.142 ± 0.012

7.779 ± 0.031

2.491 ± 0.013

3.829 ± 0.014

4.014 ± 0.021

8.635 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.868 ± 0.009

3.063 ± 0.013

6.547 ± 0.025

4.185 ± 0.021

6.636 ± 0.02

9.59 ± 0.038

5.961 ± 0.018

5.822 ± 0.017

1.183 ± 0.007

2.044 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski