Amino acid dipepetide frequency for Ensifer sp. MPMI2T

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.189AlaAla: 16.189 ± 0.131	1.042AlaCys: 1.042 ± 0.023	6.525AlaAsp: 6.525 ± 0.05	7.826AlaGlu: 7.826 ± 0.072	4.613AlaPhe: 4.613 ± 0.052	10.056AlaGly: 10.056 ± 0.07	2.218AlaHis: 2.218 ± 0.037	6.901AlaIle: 6.901 ± 0.06	4.298AlaLys: 4.298 ± 0.052	12.514AlaLeu: 12.514 ± 0.101	3.371AlaMet: 3.371 ± 0.044	3.072AlaAsn: 3.072 ± 0.045	4.735AlaPro: 4.735 ± 0.053	3.331AlaGln: 3.331 ± 0.044	8.17AlaArg: 8.17 ± 0.077	6.547AlaSer: 6.547 ± 0.06	5.861AlaThr: 5.861 ± 0.06	8.638AlaVal: 8.638 ± 0.068	1.437AlaTrp: 1.437 ± 0.027	2.537AlaTyr: 2.537 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.897CysAla: 0.897 ± 0.023	0.126CysCys: 0.126 ± 0.007	0.505CysAsp: 0.505 ± 0.015	0.486CysGlu: 0.486 ± 0.015	0.355CysPhe: 0.355 ± 0.013	0.942CysGly: 0.942 ± 0.021	0.257CysHis: 0.257 ± 0.012	0.414CysIle: 0.414 ± 0.014	0.198CysLys: 0.198 ± 0.01	0.858CysLeu: 0.858 ± 0.023	0.185CysMet: 0.185 ± 0.01	0.217CysAsn: 0.217 ± 0.01	0.431CysPro: 0.431 ± 0.017	0.233CysGln: 0.233 ± 0.011	0.661CysArg: 0.661 ± 0.022	0.503CysSer: 0.503 ± 0.015	0.41CysThr: 0.41 ± 0.016	0.604CysVal: 0.604 ± 0.017	0.121CysTrp: 0.121 ± 0.008	0.217CysTyr: 0.217 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.303AspAla: 6.303 ± 0.062	0.503AspCys: 0.503 ± 0.015	3.123AspAsp: 3.123 ± 0.046	3.637AspGlu: 3.637 ± 0.048	2.347AspPhe: 2.347 ± 0.03	5.062AspGly: 5.062 ± 0.054	1.26AspHis: 1.26 ± 0.026	3.226AspIle: 3.226 ± 0.044	1.736AspLys: 1.736 ± 0.033	5.731AspLeu: 5.731 ± 0.057	1.246AspMet: 1.246 ± 0.025	1.372AspAsn: 1.372 ± 0.028	3.31AspPro: 3.31 ± 0.04	1.692AspGln: 1.692 ± 0.03	4.374AspArg: 4.374 ± 0.052	2.101AspSer: 2.101 ± 0.032	2.416AspThr: 2.416 ± 0.039	4.165AspVal: 4.165 ± 0.049	0.941AspTrp: 0.941 ± 0.024	1.525AspTyr: 1.525 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	7.79GluAla: 7.79 ± 0.082	0.411GluCys: 0.411 ± 0.015	2.906GluAsp: 2.906 ± 0.044	3.751GluGlu: 3.751 ± 0.05	2.016GluPhe: 2.016 ± 0.031	4.415GluGly: 4.415 ± 0.043	1.302GluHis: 1.302 ± 0.027	3.99GluIle: 3.99 ± 0.047	2.633GluLys: 2.633 ± 0.04	5.744GluLeu: 5.744 ± 0.053	1.59GluMet: 1.59 ± 0.028	1.877GluAsn: 1.877 ± 0.036	2.795GluPro: 2.795 ± 0.036	2.179GluGln: 2.179 ± 0.036	5.232GluArg: 5.232 ± 0.057	2.565GluSer: 2.565 ± 0.033	3.652GluThr: 3.652 ± 0.048	3.861GluVal: 3.861 ± 0.048	0.763GluTrp: 0.763 ± 0.02	1.115GluTyr: 1.115 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.675PheAla: 4.675 ± 0.051	0.403PheCys: 0.403 ± 0.015	2.749PheAsp: 2.749 ± 0.034	2.259PheGlu: 2.259 ± 0.032	1.598PhePhe: 1.598 ± 0.031	3.647PheGly: 3.647 ± 0.051	0.818PheHis: 0.818 ± 0.02	1.806PheIle: 1.806 ± 0.028	1.13PheLys: 1.13 ± 0.026	3.679PheLeu: 3.679 ± 0.051	0.823PheMet: 0.823 ± 0.02	1.123PheAsn: 1.123 ± 0.024	1.668PhePro: 1.668 ± 0.028	1.067PheGln: 1.067 ± 0.024	2.521PheArg: 2.521 ± 0.035	2.456PheSer: 2.456 ± 0.036	1.959PheThr: 1.959 ± 0.031	3.082PheVal: 3.082 ± 0.038	0.577PheTrp: 0.577 ± 0.018	1.01PheTyr: 1.01 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	8.447GlyAla: 8.447 ± 0.072	0.832GlyCys: 0.832 ± 0.021	4.271GlyAsp: 4.271 ± 0.048	4.957GlyGlu: 4.957 ± 0.05	3.562GlyPhe: 3.562 ± 0.042	6.796GlyGly: 6.796 ± 0.089	1.904GlyHis: 1.904 ± 0.033	4.783GlyIle: 4.783 ± 0.049	3.523GlyLys: 3.523 ± 0.044	8.289GlyLeu: 8.289 ± 0.075	2.199GlyMet: 2.199 ± 0.034	2.345GlyAsn: 2.345 ± 0.042	3.224GlyPro: 3.224 ± 0.047	2.572GlyGln: 2.572 ± 0.037	6.082GlyArg: 6.082 ± 0.055	4.731GlySer: 4.731 ± 0.047	4.443GlyThr: 4.443 ± 0.051	5.842GlyVal: 5.842 ± 0.058	1.246GlyTrp: 1.246 ± 0.028	2.363GlyTyr: 2.363 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	2.269HisAla: 2.269 ± 0.034	0.246HisCys: 0.246 ± 0.01	1.246HisAsp: 1.246 ± 0.025	1.101HisGlu: 1.101 ± 0.02	0.974HisPhe: 0.974 ± 0.025	1.892HisGly: 1.892 ± 0.032	0.619HisHis: 0.619 ± 0.021	1.003HisIle: 1.003 ± 0.022	0.52HisLys: 0.52 ± 0.016	2.134HisLeu: 2.134 ± 0.033	0.541HisMet: 0.541 ± 0.014	0.502HisAsn: 0.502 ± 0.015	1.302HisPro: 1.302 ± 0.027	0.633HisGln: 0.633 ± 0.017	1.502HisArg: 1.502 ± 0.027	1.041HisSer: 1.041 ± 0.028	0.789HisThr: 0.789 ± 0.02	1.612HisVal: 1.612 ± 0.032	0.321HisTrp: 0.321 ± 0.013	0.593HisTyr: 0.593 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	7.69IleAla: 7.69 ± 0.065	0.558IleCys: 0.558 ± 0.018	3.798IleAsp: 3.798 ± 0.041	3.869IleGlu: 3.869 ± 0.044	1.947IlePhe: 1.947 ± 0.034	5.132IleGly: 5.132 ± 0.054	0.975IleHis: 0.975 ± 0.023	2.552IleIle: 2.552 ± 0.044	1.545IleLys: 1.545 ± 0.028	4.633IleLeu: 4.633 ± 0.048	1.06IleMet: 1.06 ± 0.024	1.621IleAsn: 1.621 ± 0.03	2.405IlePro: 2.405 ± 0.035	1.237IleGln: 1.237 ± 0.027	3.574IleArg: 3.574 ± 0.045	3.227IleSer: 3.227 ± 0.038	2.731IleThr: 2.731 ± 0.036	4.501IleVal: 4.501 ± 0.047	0.637IleTrp: 0.637 ± 0.018	1.248IleTyr: 1.248 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.558LysAla: 4.558 ± 0.049	0.194LysCys: 0.194 ± 0.009	1.839LysAsp: 1.839 ± 0.035	2.013LysGlu: 2.013 ± 0.028	0.997LysPhe: 0.997 ± 0.024	2.851LysGly: 2.851 ± 0.043	0.671LysHis: 0.671 ± 0.018	1.992LysIle: 1.992 ± 0.034	1.405LysLys: 1.405 ± 0.032	3.616LysLeu: 3.616 ± 0.043	0.838LysMet: 0.838 ± 0.022	0.989LysAsn: 0.989 ± 0.023	2.148LysPro: 2.148 ± 0.034	1.108LysGln: 1.108 ± 0.023	2.568LysArg: 2.568 ± 0.037	2.067LysSer: 2.067 ± 0.038	2.075LysThr: 2.075 ± 0.033	2.541LysVal: 2.541 ± 0.039	0.423LysTrp: 0.423 ± 0.015	0.67LysTyr: 0.67 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	12.918LeuAla: 12.918 ± 0.104	0.889LeuCys: 0.889 ± 0.024	5.769LeuAsp: 5.769 ± 0.059	5.49LeuGlu: 5.49 ± 0.058	3.751LeuPhe: 3.751 ± 0.05	7.737LeuGly: 7.737 ± 0.066	1.853LeuHis: 1.853 ± 0.037	5.186LeuIle: 5.186 ± 0.05	3.965LeuLys: 3.965 ± 0.046	9.222LeuLeu: 9.222 ± 0.092	2.361LeuMet: 2.361 ± 0.036	2.744LeuAsn: 2.744 ± 0.032	5.404LeuPro: 5.404 ± 0.053	2.827LeuGln: 2.827 ± 0.041	6.562LeuArg: 6.562 ± 0.063	6.726LeuSer: 6.726 ± 0.057	5.456LeuThr: 5.456 ± 0.049	7.398LeuVal: 7.398 ± 0.071	1.121LeuTrp: 1.121 ± 0.024	2.131LeuTyr: 2.131 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	2.977MetAla: 2.977 ± 0.035	0.143MetCys: 0.143 ± 0.007	1.089MetAsp: 1.089 ± 0.025	1.207MetGlu: 1.207 ± 0.026	0.707MetPhe: 0.707 ± 0.021	1.68MetGly: 1.68 ± 0.034	0.47MetHis: 0.47 ± 0.013	1.396MetIle: 1.396 ± 0.025	1.107MetLys: 1.107 ± 0.023	2.522MetLeu: 2.522 ± 0.04	0.657MetMet: 0.657 ± 0.022	0.843MetAsn: 0.843 ± 0.02	1.424MetPro: 1.424 ± 0.03	0.834MetGln: 0.834 ± 0.018	1.851MetArg: 1.851 ± 0.029	1.684MetSer: 1.684 ± 0.033	1.921MetThr: 1.921 ± 0.032	1.649MetVal: 1.649 ± 0.031	0.204MetTrp: 0.204 ± 0.01	0.317MetTyr: 0.317 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.268AsnAla: 3.268 ± 0.042	0.264AsnCys: 0.264 ± 0.013	1.594AsnAsp: 1.594 ± 0.031	1.474AsnGlu: 1.474 ± 0.025	1.066AsnPhe: 1.066 ± 0.024	2.566AsnGly: 2.566 ± 0.04	0.579AsnHis: 0.579 ± 0.018	1.452AsnIle: 1.452 ± 0.026	0.763AsnLys: 0.763 ± 0.021	2.733AsnLeu: 2.733 ± 0.04	0.655AsnMet: 0.655 ± 0.018	0.796AsnAsn: 0.796 ± 0.02	1.856AsnPro: 1.856 ± 0.031	0.815AsnGln: 0.815 ± 0.019	2.044AsnArg: 2.044 ± 0.035	1.399AsnSer: 1.399 ± 0.027	1.292AsnThr: 1.292 ± 0.025	2.055AsnVal: 2.055 ± 0.034	0.483AsnTrp: 0.483 ± 0.017	0.735AsnTyr: 0.735 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	5.6ProAla: 5.6 ± 0.056	0.317ProCys: 0.317 ± 0.013	3.362ProAsp: 3.362 ± 0.042	3.77ProGlu: 3.77 ± 0.041	1.991ProPhe: 1.991 ± 0.036	4.138ProGly: 4.138 ± 0.053	1.115ProHis: 1.115 ± 0.024	2.371ProIle: 2.371 ± 0.034	1.771ProLys: 1.771 ± 0.034	4.665ProLeu: 4.665 ± 0.052	1.125ProMet: 1.125 ± 0.023	1.373ProAsn: 1.373 ± 0.024	2.388ProPro: 2.388 ± 0.036	1.578ProGln: 1.578 ± 0.026	2.711ProArg: 2.711 ± 0.04	2.912ProSer: 2.912 ± 0.034	2.424ProThr: 2.424 ± 0.036	4.014ProVal: 4.014 ± 0.042	0.675ProTrp: 0.675 ± 0.019	1.203ProTyr: 1.203 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.739GlnAla: 3.739 ± 0.046	0.214GlnCys: 0.214 ± 0.011	1.275GlnAsp: 1.275 ± 0.025	1.622GlnGlu: 1.622 ± 0.026	1.099GlnPhe: 1.099 ± 0.022	2.044GlnGly: 2.044 ± 0.031	0.605GlnHis: 0.605 ± 0.017	1.804GlnIle: 1.804 ± 0.029	1.192GlnLys: 1.192 ± 0.025	2.847GlnLeu: 2.847 ± 0.041	0.911GlnMet: 0.911 ± 0.02	0.865GlnAsn: 0.865 ± 0.02	1.708GlnPro: 1.708 ± 0.031	1.29GlnGln: 1.29 ± 0.026	2.416GlnArg: 2.416 ± 0.039	1.877GlnSer: 1.877 ± 0.033	1.675GlnThr: 1.675 ± 0.031	1.999GlnVal: 1.999 ± 0.03	0.418GlnTrp: 0.418 ± 0.016	0.621GlnTyr: 0.621 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	7.424ArgAla: 7.424 ± 0.073	0.548ArgCys: 0.548 ± 0.021	3.937ArgAsp: 3.937 ± 0.047	4.354ArgGlu: 4.354 ± 0.059	3.002ArgPhe: 3.002 ± 0.039	4.607ArgGly: 4.607 ± 0.052	1.805ArgHis: 1.805 ± 0.027	4.141ArgIle: 4.141 ± 0.052	2.677ArgLys: 2.677 ± 0.041	7.821ArgLeu: 7.821 ± 0.066	1.915ArgMet: 1.915 ± 0.029	2.104ArgAsn: 2.104 ± 0.033	3.506ArgPro: 3.506 ± 0.044	2.66ArgGln: 2.66 ± 0.04	5.855ArgArg: 5.855 ± 0.069	4.05ArgSer: 4.05 ± 0.047	3.384ArgThr: 3.384 ± 0.037	4.616ArgVal: 4.616 ± 0.049	0.977ArgTrp: 0.977 ± 0.021	1.795ArgTyr: 1.795 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.372SerAla: 6.372 ± 0.059	0.455SerCys: 0.455 ± 0.015	3.074SerAsp: 3.074 ± 0.041	3.329SerGlu: 3.329 ± 0.041	2.439SerPhe: 2.439 ± 0.036	5.598SerGly: 5.598 ± 0.055	1.155SerHis: 1.155 ± 0.024	3.086SerIle: 3.086 ± 0.041	1.851SerLys: 1.851 ± 0.031	5.741SerLeu: 5.741 ± 0.052	1.398SerMet: 1.398 ± 0.028	1.514SerAsn: 1.514 ± 0.027	2.801SerPro: 2.801 ± 0.034	1.654SerGln: 1.654 ± 0.035	3.843SerArg: 3.843 ± 0.049	3.301SerSer: 3.301 ± 0.047	2.83SerThr: 2.83 ± 0.038	4.235SerVal: 4.235 ± 0.042	0.789SerTrp: 0.789 ± 0.019	1.412SerTyr: 1.412 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	6.1ThrAla: 6.1 ± 0.054	0.435ThrCys: 0.435 ± 0.016	2.782ThrAsp: 2.782 ± 0.036	2.842ThrGlu: 2.842 ± 0.038	2.097ThrPhe: 2.097 ± 0.034	4.897ThrGly: 4.897 ± 0.051	0.977ThrHis: 0.977 ± 0.023	3.124ThrIle: 3.124 ± 0.043	1.626ThrLys: 1.626 ± 0.032	5.387ThrLeu: 5.387 ± 0.055	1.264ThrMet: 1.264 ± 0.025	1.346ThrAsn: 1.346 ± 0.026	2.947ThrPro: 2.947 ± 0.041	1.3ThrGln: 1.3 ± 0.025	3.239ThrArg: 3.239 ± 0.038	2.921ThrSer: 2.921 ± 0.038	2.764ThrThr: 2.764 ± 0.037	4.343ThrVal: 4.343 ± 0.053	0.654ThrTrp: 0.654 ± 0.019	1.199ThrTyr: 1.199 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	8.975ValAla: 8.975 ± 0.065	0.627ValCys: 0.627 ± 0.018	4.154ValAsp: 4.154 ± 0.043	4.633ValGlu: 4.633 ± 0.048	2.948ValPhe: 2.948 ± 0.04	5.404ValGly: 5.404 ± 0.063	1.409ValHis: 1.409 ± 0.024	4.077ValIle: 4.077 ± 0.05	2.432ValLys: 2.432 ± 0.038	7.333ValLeu: 7.333 ± 0.073	1.723ValMet: 1.723 ± 0.03	2.077ValAsn: 2.077 ± 0.034	3.607ValPro: 3.607 ± 0.044	1.907ValGln: 1.907 ± 0.032	4.956ValArg: 4.956 ± 0.049	4.656ValSer: 4.656 ± 0.049	4.379ValThr: 4.379 ± 0.055	5.903ValVal: 5.903 ± 0.056	0.857ValTrp: 0.857 ± 0.021	1.497ValTyr: 1.497 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.219TrpAla: 1.219 ± 0.026	0.142TrpCys: 0.142 ± 0.008	0.612TrpAsp: 0.612 ± 0.019	0.605TrpGlu: 0.605 ± 0.016	0.53TrpPhe: 0.53 ± 0.015	0.826TrpGly: 0.826 ± 0.023	0.348TrpHis: 0.348 ± 0.013	0.678TrpIle: 0.678 ± 0.02	0.523TrpLys: 0.523 ± 0.017	1.643TrpLeu: 1.643 ± 0.033	0.355TrpMet: 0.355 ± 0.013	0.493TrpAsn: 0.493 ± 0.017	0.719TrpPro: 0.719 ± 0.019	0.568TrpGln: 0.568 ± 0.017	1.095TrpArg: 1.095 ± 0.024	0.846TrpSer: 0.846 ± 0.023	0.748TrpThr: 0.748 ± 0.022	0.756TrpVal: 0.756 ± 0.018	0.235TrpTrp: 0.235 ± 0.012	0.3TrpTyr: 0.3 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.472TyrAla: 2.472 ± 0.039	0.272TyrCys: 0.272 ± 0.01	1.46TyrAsp: 1.46 ± 0.033	1.361TyrGlu: 1.361 ± 0.027	1.012TyrPhe: 1.012 ± 0.023	2.119TyrGly: 2.119 ± 0.032	0.501TyrHis: 0.501 ± 0.016	0.959TyrIle: 0.959 ± 0.019	0.671TyrLys: 0.671 ± 0.021	2.399TyrLeu: 2.399 ± 0.037	0.464TyrMet: 0.464 ± 0.016	0.615TyrAsn: 0.615 ± 0.017	1.122TyrPro: 1.122 ± 0.024	0.735TyrGln: 0.735 ± 0.019	1.921TyrArg: 1.921 ± 0.031	1.267TyrSer: 1.267 ± 0.028	1.036TyrThr: 1.036 ± 0.023	1.742TyrVal: 1.742 ± 0.032	0.36TyrTrp: 0.36 ± 0.013	0.655TyrTyr: 0.655 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6911 proteins (2097564 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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