Wallemia mellicola

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Wallemiomycotina; Wallemiomycetes; Wallemiales; Wallemiaceae; Wallemia

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4289 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4T0T7L5|A0A4T0T7L5_9BASI MFS general substrate transporter OS=Wallemia mellicola OX=1708541 GN=E3Q01_04416 PE=4 SV=1
MM1 pKa = 7.22QFLTILSLATAAFAASVKK19 pKa = 10.26RR20 pKa = 11.84DD21 pKa = 3.21VFTDD25 pKa = 3.31SSKK28 pKa = 10.64FNQLISSPEE37 pKa = 3.89LEE39 pKa = 3.52QCYY42 pKa = 10.14QNQCLPIRR50 pKa = 11.84EE51 pKa = 4.36KK52 pKa = 10.99LRR54 pKa = 11.84SDD56 pKa = 4.16CLPIIQNTDD65 pKa = 2.55INSIDD70 pKa = 3.64TEE72 pKa = 4.41LGFEE76 pKa = 4.78FGNCVCEE83 pKa = 4.63GFDD86 pKa = 4.27FAQQNPEE93 pKa = 4.92CGDD96 pKa = 3.83CMNDD100 pKa = 4.15AIDD103 pKa = 4.16NLTSEE108 pKa = 5.51LDD110 pKa = 3.58QTCTAIRR117 pKa = 11.84SDD119 pKa = 3.62PSNPEE124 pKa = 3.68NDD126 pKa = 3.85DD127 pKa = 3.5SSSITNIPPASLLASSLLVVGISFII152 pKa = 4.29

Molecular weight: 16.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4T0RLE1|A0A4T0RLE1_9BASI RGS domain-containing protein OS=Wallemia mellicola OX=1708541 GN=E3Q01_03328 PE=4 SV=1
MM1 pKa = 6.87QLSWNLSPLQSSRR14 pKa = 11.84FSLEE18 pKa = 3.73YY19 pKa = 10.81LLLPPRR25 pKa = 11.84SALEE29 pKa = 4.0TVPLNITVKK38 pKa = 10.9ASIQSPRR45 pKa = 11.84LPTPPSIFRR54 pKa = 11.84ASSFGSGFRR63 pKa = 11.84LPWPPSCCLDD73 pKa = 3.32EE74 pKa = 4.2LTPFVVSDD82 pKa = 3.36EE83 pKa = 4.6RR84 pKa = 11.84IFRR87 pKa = 11.84HH88 pKa = 5.91LNPTIGSSRR97 pKa = 11.84IASSAYY103 pKa = 8.93QKK105 pKa = 9.96WPTRR109 pKa = 11.84HH110 pKa = 5.11FHH112 pKa = 7.18SLLKK116 pKa = 10.32FNKK119 pKa = 7.36VTRR122 pKa = 11.84ASYY125 pKa = 10.15IFKK128 pKa = 10.7VV129 pKa = 3.42

Molecular weight: 14.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4289

0

4289

2169282

58

5843

505.8

56.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.08 ± 0.033

1.001 ± 0.011

6.194 ± 0.031

6.573 ± 0.037

3.792 ± 0.019

5.607 ± 0.031

2.263 ± 0.018

5.945 ± 0.025

6.383 ± 0.037

9.3 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.981 ± 0.012

5.057 ± 0.023

5.006 ± 0.034

4.343 ± 0.028

5.166 ± 0.029

8.647 ± 0.038

5.735 ± 0.019

5.8 ± 0.023

1.11 ± 0.011

3.017 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski