Amino acid dipepetide frequency for Lactococcus phage WRP3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.106AlaAla: 0.106 ± 0.049
0.213AlaCys: 0.213 ± 0.08
2.871AlaAsp: 2.871 ± 0.307
3.243AlaGlu: 3.243 ± 0.317
1.861AlaPhe: 1.861 ± 0.191
3.562AlaGly: 3.562 ± 0.537
0.585AlaHis: 0.585 ± 0.097
3.828AlaIle: 3.828 ± 0.41
4.732AlaLys: 4.732 ± 0.556
4.492AlaLeu: 4.492 ± 0.429
1.356AlaMet: 1.356 ± 0.194
2.977AlaAsn: 2.977 ± 0.289
1.196AlaPro: 1.196 ± 0.29
2.685AlaGln: 2.685 ± 0.356
1.542AlaArg: 1.542 ± 0.262
3.535AlaSer: 3.535 ± 0.474
2.844AlaThr: 2.844 ± 0.342
3.27AlaVal: 3.27 ± 0.378
0.558AlaTrp: 0.558 ± 0.112
2.525AlaTyr: 2.525 ± 0.253
0.0AlaXaa: 0.0 ± 0.0
Cys
0.213CysAla: 0.213 ± 0.08
0.053CysCys: 0.053 ± 0.046
0.372CysAsp: 0.372 ± 0.112
0.478CysGlu: 0.478 ± 0.136
0.213CysPhe: 0.213 ± 0.075
0.558CysGly: 0.558 ± 0.14
0.0CysHis: 0.0 ± 0.0
0.452CysIle: 0.452 ± 0.111
0.611CysLys: 0.611 ± 0.125
0.452CysLeu: 0.452 ± 0.11
0.106CysMet: 0.106 ± 0.062
0.399CysAsn: 0.399 ± 0.104
0.292CysPro: 0.292 ± 0.115
0.213CysGln: 0.213 ± 0.086
0.425CysArg: 0.425 ± 0.103
0.691CysSer: 0.691 ± 0.153
0.186CysThr: 0.186 ± 0.068
0.372CysVal: 0.372 ± 0.104
0.053CysTrp: 0.053 ± 0.041
0.346CysTyr: 0.346 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
2.791AspAla: 2.791 ± 0.465
0.292AspCys: 0.292 ± 0.104
4.705AspAsp: 4.705 ± 0.431
5.928AspGlu: 5.928 ± 0.492
4.04AspPhe: 4.04 ± 0.298
4.678AspGly: 4.678 ± 0.399
0.478AspHis: 0.478 ± 0.124
5.848AspIle: 5.848 ± 0.387
6.38AspLys: 6.38 ± 0.439
5.423AspLeu: 5.423 ± 0.392
1.728AspMet: 1.728 ± 0.231
4.944AspAsn: 4.944 ± 0.425
1.01AspPro: 1.01 ± 0.175
0.93AspGln: 0.93 ± 0.18
2.127AspArg: 2.127 ± 0.206
4.678AspSer: 4.678 ± 0.368
4.014AspThr: 4.014 ± 0.336
4.226AspVal: 4.226 ± 0.373
0.797AspTrp: 0.797 ± 0.141
3.642AspTyr: 3.642 ± 0.35
0.0AspXaa: 0.0 ± 0.0
Glu
4.014GluAla: 4.014 ± 0.357
0.558GluCys: 0.558 ± 0.134
5.795GluAsp: 5.795 ± 0.52
6.964GluGlu: 6.964 ± 0.591
3.589GluPhe: 3.589 ± 0.353
3.19GluGly: 3.19 ± 0.225
1.196GluHis: 1.196 ± 0.259
6.964GluIle: 6.964 ± 0.465
7.204GluLys: 7.204 ± 0.59
7.735GluLeu: 7.735 ± 0.5
2.313GluMet: 2.313 ± 0.24
4.785GluAsn: 4.785 ± 0.312
1.302GluPro: 1.302 ± 0.196
2.419GluGln: 2.419 ± 0.232
2.871GluArg: 2.871 ± 0.295
5.476GluSer: 5.476 ± 0.34
4.333GluThr: 4.333 ± 0.394
5.077GluVal: 5.077 ± 0.413
0.771GluTrp: 0.771 ± 0.161
4.439GluTyr: 4.439 ± 0.412
0.0GluXaa: 0.0 ± 0.0
Phe
2.1PheAla: 2.1 ± 0.284
0.399PheCys: 0.399 ± 0.122
3.987PheAsp: 3.987 ± 0.351
3.775PheGlu: 3.775 ± 0.363
1.196PhePhe: 1.196 ± 0.189
2.711PheGly: 2.711 ± 0.269
0.532PheHis: 0.532 ± 0.141
3.11PheIle: 3.11 ± 0.271
4.04PheLys: 4.04 ± 0.354
3.11PheLeu: 3.11 ± 0.339
1.356PheMet: 1.356 ± 0.204
3.27PheAsn: 3.27 ± 0.263
0.877PhePro: 0.877 ± 0.155
1.276PheGln: 1.276 ± 0.182
1.595PheArg: 1.595 ± 0.268
2.924PheSer: 2.924 ± 0.246
2.924PheThr: 2.924 ± 0.275
2.578PheVal: 2.578 ± 0.306
0.372PheTrp: 0.372 ± 0.112
2.499PheTyr: 2.499 ± 0.322
0.0PheXaa: 0.0 ± 0.0
Gly
2.499GlyAla: 2.499 ± 0.4
0.292GlyCys: 0.292 ± 0.1
3.402GlyAsp: 3.402 ± 0.282
4.226GlyGlu: 4.226 ± 0.318
3.216GlyPhe: 3.216 ± 0.279
3.562GlyGly: 3.562 ± 0.489
0.904GlyHis: 0.904 ± 0.131
4.997GlyIle: 4.997 ± 0.428
4.678GlyLys: 4.678 ± 0.342
4.864GlyLeu: 4.864 ± 0.363
1.542GlyMet: 1.542 ± 0.168
4.04GlyAsn: 4.04 ± 0.355
0.08GlyPro: 0.08 ± 0.051
1.382GlyGln: 1.382 ± 0.183
2.02GlyArg: 2.02 ± 0.245
3.695GlySer: 3.695 ± 0.352
4.226GlyThr: 4.226 ± 0.465
3.748GlyVal: 3.748 ± 0.286
0.797GlyTrp: 0.797 ± 0.19
2.738GlyTyr: 2.738 ± 0.214
0.0GlyXaa: 0.0 ± 0.0
His
0.665HisAla: 0.665 ± 0.124
0.08HisCys: 0.08 ± 0.049
1.037HisAsp: 1.037 ± 0.185
1.063HisGlu: 1.063 ± 0.206
0.797HisPhe: 0.797 ± 0.177
1.01HisGly: 1.01 ± 0.195
0.319HisHis: 0.319 ± 0.097
1.223HisIle: 1.223 ± 0.198
1.223HisLys: 1.223 ± 0.215
0.904HisLeu: 0.904 ± 0.213
0.319HisMet: 0.319 ± 0.091
1.01HisAsn: 1.01 ± 0.212
0.399HisPro: 0.399 ± 0.1
0.532HisGln: 0.532 ± 0.113
0.425HisArg: 0.425 ± 0.112
0.93HisSer: 0.93 ± 0.158
0.824HisThr: 0.824 ± 0.133
0.665HisVal: 0.665 ± 0.146
0.213HisTrp: 0.213 ± 0.072
0.665HisTyr: 0.665 ± 0.176
0.0HisXaa: 0.0 ± 0.0
Ile
3.695IleAla: 3.695 ± 0.314
0.532IleCys: 0.532 ± 0.13
5.768IleAsp: 5.768 ± 0.313
6.459IleGlu: 6.459 ± 0.473
3.19IlePhe: 3.19 ± 0.394
3.589IleGly: 3.589 ± 0.31
1.143IleHis: 1.143 ± 0.187
5.529IleIle: 5.529 ± 0.467
7.788IleLys: 7.788 ± 0.448
5.875IleLeu: 5.875 ± 0.38
1.542IleMet: 1.542 ± 0.206
5.768IleAsn: 5.768 ± 0.465
2.632IlePro: 2.632 ± 0.259
2.658IleGln: 2.658 ± 0.374
2.525IleArg: 2.525 ± 0.277
5.13IleSer: 5.13 ± 0.376
4.732IleThr: 4.732 ± 0.408
4.971IleVal: 4.971 ± 0.36
0.691IleTrp: 0.691 ± 0.147
2.871IleTyr: 2.871 ± 0.316
0.0IleXaa: 0.0 ± 0.0
Lys
4.732LysAla: 4.732 ± 0.481
0.532LysCys: 0.532 ± 0.144
6.619LysAsp: 6.619 ± 0.393
9.782LysGlu: 9.782 ± 0.639
3.456LysPhe: 3.456 ± 0.383
4.492LysGly: 4.492 ± 0.348
1.435LysHis: 1.435 ± 0.231
7.177LysIle: 7.177 ± 0.471
7.576LysLys: 7.576 ± 0.613
8.028LysLeu: 8.028 ± 0.477
3.03LysMet: 3.03 ± 0.291
5.715LysAsn: 5.715 ± 0.397
2.233LysPro: 2.233 ± 0.28
3.163LysGln: 3.163 ± 0.357
3.615LysArg: 3.615 ± 0.309
4.439LysSer: 4.439 ± 0.425
5.343LysThr: 5.343 ± 0.41
4.785LysVal: 4.785 ± 0.349
1.037LysTrp: 1.037 ± 0.163
3.642LysTyr: 3.642 ± 0.268
0.0LysXaa: 0.0 ± 0.0
Leu
4.226LeuAla: 4.226 ± 0.328
0.558LeuCys: 0.558 ± 0.105
5.635LeuAsp: 5.635 ± 0.43
6.831LeuGlu: 6.831 ± 0.441
3.642LeuPhe: 3.642 ± 0.4
4.811LeuGly: 4.811 ± 0.448
1.037LeuHis: 1.037 ± 0.199
5.502LeuIle: 5.502 ± 0.401
7.762LeuLys: 7.762 ± 0.645
6.433LeuLeu: 6.433 ± 0.533
1.967LeuMet: 1.967 ± 0.241
5.662LeuAsn: 5.662 ± 0.409
2.153LeuPro: 2.153 ± 0.216
2.499LeuGln: 2.499 ± 0.316
3.057LeuArg: 3.057 ± 0.301
6.247LeuSer: 6.247 ± 0.41
5.369LeuThr: 5.369 ± 0.396
4.678LeuVal: 4.678 ± 0.351
0.611LeuTrp: 0.611 ± 0.133
3.296LeuTyr: 3.296 ± 0.402
0.0LeuXaa: 0.0 ± 0.0
Met
1.515MetAla: 1.515 ± 0.162
0.186MetCys: 0.186 ± 0.077
1.542MetAsp: 1.542 ± 0.187
2.1MetGlu: 2.1 ± 0.231
1.143MetPhe: 1.143 ± 0.182
1.249MetGly: 1.249 ± 0.18
0.239MetHis: 0.239 ± 0.081
1.887MetIle: 1.887 ± 0.189
2.366MetLys: 2.366 ± 0.243
1.808MetLeu: 1.808 ± 0.194
0.558MetMet: 0.558 ± 0.125
1.967MetAsn: 1.967 ± 0.211
0.824MetPro: 0.824 ± 0.142
1.09MetGln: 1.09 ± 0.314
0.877MetArg: 0.877 ± 0.191
1.994MetSer: 1.994 ± 0.246
1.994MetThr: 1.994 ± 0.221
1.701MetVal: 1.701 ± 0.208
0.266MetTrp: 0.266 ± 0.082
1.17MetTyr: 1.17 ± 0.166
0.0MetXaa: 0.0 ± 0.0
Asn
3.137AsnAla: 3.137 ± 0.314
0.425AsnCys: 0.425 ± 0.118
3.642AsnAsp: 3.642 ± 0.339
5.183AsnGlu: 5.183 ± 0.419
2.951AsnPhe: 2.951 ± 0.259
4.545AsnGly: 4.545 ± 0.348
0.824AsnHis: 0.824 ± 0.157
5.821AsnIle: 5.821 ± 0.466
6.247AsnLys: 6.247 ± 0.456
5.369AsnLeu: 5.369 ± 0.324
1.568AsnMet: 1.568 ± 0.209
4.014AsnAsn: 4.014 ± 0.37
1.808AsnPro: 1.808 ± 0.205
2.685AsnGln: 2.685 ± 0.352
2.047AsnArg: 2.047 ± 0.201
4.625AsnSer: 4.625 ± 0.324
3.695AsnThr: 3.695 ± 0.321
3.828AsnVal: 3.828 ± 0.303
0.771AsnTrp: 0.771 ± 0.147
2.685AsnTyr: 2.685 ± 0.256
0.0AsnXaa: 0.0 ± 0.0
Pro
1.356ProAla: 1.356 ± 0.275
0.106ProCys: 0.106 ± 0.052
1.701ProAsp: 1.701 ± 0.229
1.595ProGlu: 1.595 ± 0.186
1.17ProPhe: 1.17 ± 0.156
0.133ProGly: 0.133 ± 0.057
0.399ProHis: 0.399 ± 0.104
1.595ProIle: 1.595 ± 0.206
2.206ProLys: 2.206 ± 0.207
2.259ProLeu: 2.259 ± 0.238
0.665ProMet: 0.665 ± 0.142
1.701ProAsn: 1.701 ± 0.203
0.372ProPro: 0.372 ± 0.083
0.611ProGln: 0.611 ± 0.126
0.638ProArg: 0.638 ± 0.164
1.754ProSer: 1.754 ± 0.294
1.542ProThr: 1.542 ± 0.285
1.781ProVal: 1.781 ± 0.234
0.159ProTrp: 0.159 ± 0.086
1.143ProTyr: 1.143 ± 0.185
0.0ProXaa: 0.0 ± 0.0
Gln
2.127GlnAla: 2.127 ± 0.42
0.186GlnCys: 0.186 ± 0.077
1.701GlnAsp: 1.701 ± 0.195
2.286GlnGlu: 2.286 ± 0.213
1.223GlnPhe: 1.223 ± 0.204
1.967GlnGly: 1.967 ± 0.221
0.744GlnHis: 0.744 ± 0.174
2.392GlnIle: 2.392 ± 0.255
3.19GlnLys: 3.19 ± 0.381
3.27GlnLeu: 3.27 ± 0.432
1.302GlnMet: 1.302 ± 0.25
1.967GlnAsn: 1.967 ± 0.269
0.611GlnPro: 0.611 ± 0.121
1.382GlnGln: 1.382 ± 0.452
1.382GlnArg: 1.382 ± 0.177
2.047GlnSer: 2.047 ± 0.236
2.047GlnThr: 2.047 ± 0.265
1.356GlnVal: 1.356 ± 0.165
0.452GlnTrp: 0.452 ± 0.124
1.435GlnTyr: 1.435 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
1.382ArgAla: 1.382 ± 0.148
0.346ArgCys: 0.346 ± 0.152
2.738ArgAsp: 2.738 ± 0.255
2.924ArgGlu: 2.924 ± 0.307
1.621ArgPhe: 1.621 ± 0.187
2.286ArgGly: 2.286 ± 0.23
0.718ArgHis: 0.718 ± 0.133
2.764ArgIle: 2.764 ± 0.304
3.429ArgLys: 3.429 ± 0.359
2.605ArgLeu: 2.605 ± 0.276
0.638ArgMet: 0.638 ± 0.124
2.366ArgAsn: 2.366 ± 0.276
0.718ArgPro: 0.718 ± 0.128
1.09ArgGln: 1.09 ± 0.168
1.143ArgArg: 1.143 ± 0.18
1.834ArgSer: 1.834 ± 0.228
2.127ArgThr: 2.127 ± 0.212
2.313ArgVal: 2.313 ± 0.317
0.505ArgTrp: 0.505 ± 0.116
1.834ArgTyr: 1.834 ± 0.217
0.0ArgXaa: 0.0 ± 0.0
Ser
3.668SerAla: 3.668 ± 0.308
0.399SerCys: 0.399 ± 0.118
4.625SerAsp: 4.625 ± 0.397
5.237SerGlu: 5.237 ± 0.348
3.402SerPhe: 3.402 ± 0.366
4.333SerGly: 4.333 ± 0.469
0.771SerHis: 0.771 ± 0.135
5.13SerIle: 5.13 ± 0.415
5.742SerLys: 5.742 ± 0.372
5.13SerLeu: 5.13 ± 0.648
1.834SerMet: 1.834 ± 0.224
3.961SerAsn: 3.961 ± 0.389
1.595SerPro: 1.595 ± 0.174
2.233SerGln: 2.233 ± 0.249
2.685SerArg: 2.685 ± 0.315
4.678SerSer: 4.678 ± 0.613
3.695SerThr: 3.695 ± 0.266
3.828SerVal: 3.828 ± 0.337
0.718SerTrp: 0.718 ± 0.17
2.871SerTyr: 2.871 ± 0.319
0.0SerXaa: 0.0 ± 0.0
Thr
3.535ThrAla: 3.535 ± 0.421
0.372ThrCys: 0.372 ± 0.129
3.934ThrAsp: 3.934 ± 0.322
3.482ThrGlu: 3.482 ± 0.291
2.818ThrPhe: 2.818 ± 0.313
3.482ThrGly: 3.482 ± 0.387
1.249ThrHis: 1.249 ± 0.198
4.732ThrIle: 4.732 ± 0.409
5.795ThrLys: 5.795 ± 0.339
5.29ThrLeu: 5.29 ± 0.391
1.595ThrMet: 1.595 ± 0.184
3.615ThrAsn: 3.615 ± 0.314
1.967ThrPro: 1.967 ± 0.224
1.861ThrGln: 1.861 ± 0.256
1.94ThrArg: 1.94 ± 0.23
4.147ThrSer: 4.147 ± 0.354
3.668ThrThr: 3.668 ± 0.527
4.838ThrVal: 4.838 ± 0.377
0.824ThrTrp: 0.824 ± 0.183
2.951ThrTyr: 2.951 ± 0.248
0.0ThrXaa: 0.0 ± 0.0
Val
3.429ValAla: 3.429 ± 0.427
0.452ValCys: 0.452 ± 0.133
4.732ValAsp: 4.732 ± 0.317
4.944ValGlu: 4.944 ± 0.409
2.844ValPhe: 2.844 ± 0.27
3.349ValGly: 3.349 ± 0.331
0.824ValHis: 0.824 ± 0.159
4.359ValIle: 4.359 ± 0.442
5.29ValLys: 5.29 ± 0.389
4.545ValLeu: 4.545 ± 0.381
1.542ValMet: 1.542 ± 0.191
3.589ValAsn: 3.589 ± 0.322
1.462ValPro: 1.462 ± 0.206
2.02ValGln: 2.02 ± 0.232
2.18ValArg: 2.18 ± 0.238
4.014ValSer: 4.014 ± 0.381
4.545ValThr: 4.545 ± 0.385
3.987ValVal: 3.987 ± 0.349
0.638ValTrp: 0.638 ± 0.132
2.658ValTyr: 2.658 ± 0.313
0.0ValXaa: 0.0 ± 0.0
Trp
0.399TrpAla: 0.399 ± 0.1
0.08TrpCys: 0.08 ± 0.048
0.877TrpAsp: 0.877 ± 0.145
0.877TrpGlu: 0.877 ± 0.18
0.505TrpPhe: 0.505 ± 0.115
0.718TrpGly: 0.718 ± 0.145
0.266TrpHis: 0.266 ± 0.079
0.665TrpIle: 0.665 ± 0.16
0.771TrpLys: 0.771 ± 0.158
0.718TrpLeu: 0.718 ± 0.142
0.532TrpMet: 0.532 ± 0.114
1.09TrpAsn: 1.09 ± 0.176
0.0TrpPro: 0.0 ± 0.0
0.399TrpGln: 0.399 ± 0.097
0.372TrpArg: 0.372 ± 0.104
0.665TrpSer: 0.665 ± 0.166
0.744TrpThr: 0.744 ± 0.129
0.532TrpVal: 0.532 ± 0.134
0.133TrpTrp: 0.133 ± 0.06
0.399TrpTyr: 0.399 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.392TyrAla: 2.392 ± 0.226
0.425TyrCys: 0.425 ± 0.128
2.977TyrAsp: 2.977 ± 0.281
3.615TyrGlu: 3.615 ± 0.446
1.754TyrPhe: 1.754 ± 0.269
2.685TyrGly: 2.685 ± 0.248
0.638TyrHis: 0.638 ± 0.135
3.11TyrIle: 3.11 ± 0.329
3.801TyrLys: 3.801 ± 0.318
3.668TyrLeu: 3.668 ± 0.361
0.957TyrMet: 0.957 ± 0.143
3.03TyrAsn: 3.03 ± 0.319
1.356TyrPro: 1.356 ± 0.251
1.94TyrGln: 1.94 ± 0.196
1.94TyrArg: 1.94 ± 0.25
3.03TyrSer: 3.03 ± 0.307
3.243TyrThr: 3.243 ± 0.263
2.871TyrVal: 2.871 ± 0.318
0.372TyrTrp: 0.372 ± 0.089
1.887TyrTyr: 1.887 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 178 proteins (37621 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski