Nocardioides zeae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P0HJL2|A0A6P0HJL2_9ACTN S8 family serine peptidase OS=Nocardioides zeae OX=1457234 GN=G3T38_10995 PE=3 SV=1
MM1 pKa = 7.77SDD3 pKa = 3.67QPPTPYY9 pKa = 9.93PGEE12 pKa = 4.44PGPYY16 pKa = 9.34AAPHH20 pKa = 6.23PPSYY24 pKa = 10.25PVGSLAPQHH33 pKa = 6.43LAPPPPSLPTDD44 pKa = 4.1GPSPHH49 pKa = 6.95APAGDD54 pKa = 3.76GSRR57 pKa = 11.84LASAALALACLPLGVTNALGVVLGAVGMAAGPRR90 pKa = 11.84VRR92 pKa = 11.84PRR94 pKa = 11.84WGMSVAAVVVGTLWMAAGTGIGVAVWAGDD123 pKa = 3.62RR124 pKa = 11.84DD125 pKa = 4.05GSADD129 pKa = 3.91PDD131 pKa = 3.63TAARR135 pKa = 11.84VAEE138 pKa = 4.84LGDD141 pKa = 4.43EE142 pKa = 4.19IDD144 pKa = 4.93DD145 pKa = 4.44LRR147 pKa = 11.84GDD149 pKa = 4.52LFAAMGPGGAYY160 pKa = 10.15SDD162 pKa = 4.05TEE164 pKa = 4.33VLSEE168 pKa = 5.56LDD170 pKa = 4.94DD171 pKa = 4.25EE172 pKa = 4.89DD173 pKa = 3.46EE174 pKa = 4.28RR175 pKa = 11.84AAVEE179 pKa = 4.46LLAEE183 pKa = 4.55DD184 pKa = 4.42VPVADD189 pKa = 5.14LATGACVLDD198 pKa = 3.84PALNQVLWTGEE209 pKa = 3.98HH210 pKa = 6.71LPTVLATVDD219 pKa = 3.54CADD222 pKa = 3.27VHH224 pKa = 6.01DD225 pKa = 4.9AEE227 pKa = 4.78MVGSITVPDD236 pKa = 5.39RR237 pKa = 11.84IAEE240 pKa = 4.54DD241 pKa = 3.77GWDD244 pKa = 3.74SDD246 pKa = 4.38PFLDD250 pKa = 5.16FAWDD254 pKa = 3.42ACDD257 pKa = 4.6DD258 pKa = 3.72AFYY261 pKa = 10.71DD262 pKa = 3.98YY263 pKa = 11.49AGDD266 pKa = 3.65AGYY269 pKa = 10.12WDD271 pKa = 4.38AEE273 pKa = 3.96EE274 pKa = 4.58TYY276 pKa = 11.12AAFVPPSEE284 pKa = 4.48EE285 pKa = 3.5AWDD288 pKa = 3.89AGEE291 pKa = 4.3RR292 pKa = 11.84AIACFASSEE301 pKa = 4.12GLTSGSLRR309 pKa = 11.84AVEE312 pKa = 4.39AGG314 pKa = 3.25

Molecular weight: 32.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P0HHK6|A0A6P0HHK6_9ACTN WYL domain-containing protein OS=Nocardioides zeae OX=1457234 GN=G3T38_02365 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 4.97KK15 pKa = 10.04VHH17 pKa = 6.59GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIITARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.09GRR40 pKa = 11.84KK41 pKa = 9.0SLTVV45 pKa = 3.12

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4048

0

4048

1324490

32

1659

327.2

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.174 ± 0.057

0.667 ± 0.011

6.558 ± 0.03

5.755 ± 0.033

2.561 ± 0.02

9.573 ± 0.033

2.117 ± 0.02

2.926 ± 0.023

1.493 ± 0.026

10.413 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.594 ± 0.014

1.499 ± 0.018

5.844 ± 0.031

2.497 ± 0.019

7.883 ± 0.041

4.842 ± 0.024

6.154 ± 0.033

10.157 ± 0.04

1.473 ± 0.015

1.819 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski