Amino acid dipepetide frequency for Staphylococcus phage phiIPLA-C1C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.049AlaAla: 0.049 ± 0.034
0.146AlaCys: 0.146 ± 0.061
2.245AlaAsp: 2.245 ± 0.268
2.44AlaGlu: 2.44 ± 0.309
1.561AlaPhe: 1.561 ± 0.205
1.903AlaGly: 1.903 ± 0.256
0.83AlaHis: 0.83 ± 0.134
2.708AlaIle: 2.708 ± 0.26
4.245AlaLys: 4.245 ± 0.295
3.367AlaLeu: 3.367 ± 0.305
1.0AlaMet: 1.0 ± 0.211
2.025AlaAsn: 2.025 ± 0.229
1.147AlaPro: 1.147 ± 0.186
1.513AlaGln: 1.513 ± 0.211
1.439AlaArg: 1.439 ± 0.2
2.781AlaSer: 2.781 ± 0.346
2.708AlaThr: 2.708 ± 0.323
2.147AlaVal: 2.147 ± 0.245
0.439AlaTrp: 0.439 ± 0.094
1.854AlaTyr: 1.854 ± 0.216
0.0AlaXaa: 0.0 ± 0.0
Cys
0.268CysAla: 0.268 ± 0.076
0.122CysCys: 0.122 ± 0.059
0.244CysAsp: 0.244 ± 0.077
0.366CysGlu: 0.366 ± 0.093
0.268CysPhe: 0.268 ± 0.085
0.488CysGly: 0.488 ± 0.117
0.122CysHis: 0.122 ± 0.05
0.39CysIle: 0.39 ± 0.108
0.537CysLys: 0.537 ± 0.115
0.512CysLeu: 0.512 ± 0.117
0.171CysMet: 0.171 ± 0.068
0.244CysAsn: 0.244 ± 0.075
0.22CysPro: 0.22 ± 0.07
0.244CysGln: 0.244 ± 0.079
0.146CysArg: 0.146 ± 0.061
0.415CysSer: 0.415 ± 0.096
0.22CysThr: 0.22 ± 0.066
0.342CysVal: 0.342 ± 0.103
0.073CysTrp: 0.073 ± 0.042
0.415CysTyr: 0.415 ± 0.092
0.0CysXaa: 0.0 ± 0.0
Asp
2.415AspAla: 2.415 ± 0.282
0.293AspCys: 0.293 ± 0.092
4.343AspAsp: 4.343 ± 0.368
4.757AspGlu: 4.757 ± 0.357
3.147AspPhe: 3.147 ± 0.279
2.806AspGly: 2.806 ± 0.29
0.464AspHis: 0.464 ± 0.105
6.66AspIle: 6.66 ± 0.447
7.612AspLys: 7.612 ± 0.48
6.099AspLeu: 6.099 ± 0.48
1.659AspMet: 1.659 ± 0.176
5.685AspAsn: 5.685 ± 0.404
1.342AspPro: 1.342 ± 0.195
0.854AspGln: 0.854 ± 0.156
2.196AspArg: 2.196 ± 0.227
4.416AspSer: 4.416 ± 0.323
4.44AspThr: 4.44 ± 0.39
4.343AspVal: 4.343 ± 0.369
0.561AspTrp: 0.561 ± 0.102
4.05AspTyr: 4.05 ± 0.301
0.0AspXaa: 0.0 ± 0.0
Glu
3.196GluAla: 3.196 ± 0.293
0.415GluCys: 0.415 ± 0.112
6.026GluAsp: 6.026 ± 0.509
8.466GluGlu: 8.466 ± 0.536
3.855GluPhe: 3.855 ± 0.268
4.001GluGly: 4.001 ± 0.408
1.269GluHis: 1.269 ± 0.207
6.075GluIle: 6.075 ± 0.515
7.368GluLys: 7.368 ± 0.504
7.588GluLeu: 7.588 ± 0.556
1.781GluMet: 1.781 ± 0.226
5.245GluAsn: 5.245 ± 0.372
1.683GluPro: 1.683 ± 0.254
3.416GluGln: 3.416 ± 0.34
3.05GluArg: 3.05 ± 0.304
4.733GluSer: 4.733 ± 0.332
3.977GluThr: 3.977 ± 0.421
5.27GluVal: 5.27 ± 0.323
0.634GluTrp: 0.634 ± 0.128
4.172GluTyr: 4.172 ± 0.338
0.0GluXaa: 0.0 ± 0.0
Phe
1.244PheAla: 1.244 ± 0.178
0.293PheCys: 0.293 ± 0.075
2.757PheAsp: 2.757 ± 0.304
2.976PheGlu: 2.976 ± 0.259
1.439PhePhe: 1.439 ± 0.195
1.708PheGly: 1.708 ± 0.207
0.439PheHis: 0.439 ± 0.118
3.684PheIle: 3.684 ± 0.349
4.636PheLys: 4.636 ± 0.318
3.245PheLeu: 3.245 ± 0.319
0.781PheMet: 0.781 ± 0.128
4.27PheAsn: 4.27 ± 0.353
0.732PhePro: 0.732 ± 0.117
0.878PheGln: 0.878 ± 0.153
1.049PheArg: 1.049 ± 0.152
2.733PheSer: 2.733 ± 0.264
2.464PheThr: 2.464 ± 0.22
2.367PheVal: 2.367 ± 0.241
0.293PheTrp: 0.293 ± 0.085
2.513PheTyr: 2.513 ± 0.324
0.0PheXaa: 0.0 ± 0.0
Gly
1.976GlyAla: 1.976 ± 0.287
0.366GlyCys: 0.366 ± 0.088
3.66GlyAsp: 3.66 ± 0.282
3.196GlyGlu: 3.196 ± 0.356
1.854GlyPhe: 1.854 ± 0.191
3.367GlyGly: 3.367 ± 0.553
0.756GlyHis: 0.756 ± 0.141
4.489GlyIle: 4.489 ± 0.472
5.27GlyLys: 5.27 ± 0.524
4.855GlyLeu: 4.855 ± 0.377
1.171GlyMet: 1.171 ± 0.197
3.44GlyAsn: 3.44 ± 0.316
0.0GlyPro: 0.0 ± 0.0
1.635GlyGln: 1.635 ± 0.184
2.293GlyArg: 2.293 ± 0.405
3.513GlySer: 3.513 ± 0.419
3.513GlyThr: 3.513 ± 0.374
3.733GlyVal: 3.733 ± 0.32
0.464GlyTrp: 0.464 ± 0.084
3.66GlyTyr: 3.66 ± 0.276
0.0GlyXaa: 0.0 ± 0.0
His
0.464HisAla: 0.464 ± 0.139
0.22HisCys: 0.22 ± 0.07
0.878HisAsp: 0.878 ± 0.119
0.854HisGlu: 0.854 ± 0.144
0.927HisPhe: 0.927 ± 0.154
1.22HisGly: 1.22 ± 0.18
0.366HisHis: 0.366 ± 0.105
1.561HisIle: 1.561 ± 0.209
1.342HisLys: 1.342 ± 0.165
1.464HisLeu: 1.464 ± 0.209
0.317HisMet: 0.317 ± 0.083
1.22HisAsn: 1.22 ± 0.194
0.586HisPro: 0.586 ± 0.111
0.439HisGln: 0.439 ± 0.094
0.61HisArg: 0.61 ± 0.128
0.756HisSer: 0.756 ± 0.137
0.708HisThr: 0.708 ± 0.118
0.878HisVal: 0.878 ± 0.142
0.122HisTrp: 0.122 ± 0.082
0.878HisTyr: 0.878 ± 0.156
0.0HisXaa: 0.0 ± 0.0
Ile
3.123IleAla: 3.123 ± 0.355
0.488IleCys: 0.488 ± 0.111
6.368IleAsp: 6.368 ± 0.35
7.075IleGlu: 7.075 ± 0.479
2.464IlePhe: 2.464 ± 0.248
3.733IleGly: 3.733 ± 0.376
1.366IleHis: 1.366 ± 0.218
5.782IleIle: 5.782 ± 0.537
8.856IleLys: 8.856 ± 0.52
6.099IleLeu: 6.099 ± 0.436
1.366IleMet: 1.366 ± 0.185
6.124IleAsn: 6.124 ± 0.45
2.171IlePro: 2.171 ± 0.21
2.318IleGln: 2.318 ± 0.248
2.611IleArg: 2.611 ± 0.23
5.465IleSer: 5.465 ± 0.342
4.611IleThr: 4.611 ± 0.321
4.855IleVal: 4.855 ± 0.386
0.342IleTrp: 0.342 ± 0.085
3.196IleTyr: 3.196 ± 0.322
0.0IleXaa: 0.0 ± 0.0
Lys
3.879LysAla: 3.879 ± 0.399
0.634LysCys: 0.634 ± 0.125
7.734LysAsp: 7.734 ± 0.435
11.174LysGlu: 11.174 ± 0.695
3.269LysPhe: 3.269 ± 0.262
6.075LysGly: 6.075 ± 0.623
1.903LysHis: 1.903 ± 0.244
5.929LysIle: 5.929 ± 0.382
9.735LysLys: 9.735 ± 0.653
7.246LysLeu: 7.246 ± 0.447
2.318LysMet: 2.318 ± 0.209
7.222LysAsn: 7.222 ± 0.499
2.489LysPro: 2.489 ± 0.25
3.879LysGln: 3.879 ± 0.36
4.001LysArg: 4.001 ± 0.306
6.075LysSer: 6.075 ± 0.405
4.733LysThr: 4.733 ± 0.361
6.417LysVal: 6.417 ± 0.407
0.927LysTrp: 0.927 ± 0.177
5.221LysTyr: 5.221 ± 0.451
0.0LysXaa: 0.0 ± 0.0
Leu
2.952LeuAla: 2.952 ± 0.275
0.317LeuCys: 0.317 ± 0.083
6.148LeuAsp: 6.148 ± 0.399
7.514LeuGlu: 7.514 ± 0.497
2.854LeuPhe: 2.854 ± 0.245
4.831LeuGly: 4.831 ± 0.423
1.415LeuHis: 1.415 ± 0.191
5.977LeuIle: 5.977 ± 0.503
8.027LeuLys: 8.027 ± 0.546
6.148LeuLeu: 6.148 ± 0.573
2.049LeuMet: 2.049 ± 0.209
5.953LeuAsn: 5.953 ± 0.361
2.367LeuPro: 2.367 ± 0.242
2.611LeuGln: 2.611 ± 0.257
2.903LeuArg: 2.903 ± 0.254
5.709LeuSer: 5.709 ± 0.41
4.782LeuThr: 4.782 ± 0.408
4.904LeuVal: 4.904 ± 0.326
0.366LeuTrp: 0.366 ± 0.093
3.904LeuTyr: 3.904 ± 0.318
0.0LeuXaa: 0.0 ± 0.0
Met
1.342MetAla: 1.342 ± 0.219
0.049MetCys: 0.049 ± 0.037
1.147MetAsp: 1.147 ± 0.168
1.732MetGlu: 1.732 ± 0.205
0.854MetPhe: 0.854 ± 0.146
0.951MetGly: 0.951 ± 0.189
0.195MetHis: 0.195 ± 0.063
1.293MetIle: 1.293 ± 0.209
2.367MetLys: 2.367 ± 0.237
1.732MetLeu: 1.732 ± 0.191
0.464MetMet: 0.464 ± 0.087
1.586MetAsn: 1.586 ± 0.207
0.683MetPro: 0.683 ± 0.113
0.683MetGln: 0.683 ± 0.14
1.293MetArg: 1.293 ± 0.157
1.586MetSer: 1.586 ± 0.188
1.635MetThr: 1.635 ± 0.219
1.049MetVal: 1.049 ± 0.169
0.098MetTrp: 0.098 ± 0.046
1.0MetTyr: 1.0 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
2.684AsnAla: 2.684 ± 0.304
0.439AsnCys: 0.439 ± 0.114
4.172AsnAsp: 4.172 ± 0.306
4.733AsnGlu: 4.733 ± 0.334
3.123AsnPhe: 3.123 ± 0.305
3.855AsnGly: 3.855 ± 0.268
1.439AsnHis: 1.439 ± 0.181
6.173AsnIle: 6.173 ± 0.484
8.198AsnLys: 8.198 ± 0.527
5.416AsnLeu: 5.416 ± 0.43
1.513AsnMet: 1.513 ± 0.16
6.856AsnAsn: 6.856 ± 0.531
2.44AsnPro: 2.44 ± 0.249
2.123AsnGln: 2.123 ± 0.251
2.391AsnArg: 2.391 ± 0.257
4.733AsnSer: 4.733 ± 0.368
4.587AsnThr: 4.587 ± 0.326
4.172AsnVal: 4.172 ± 0.329
0.781AsnTrp: 0.781 ± 0.143
4.05AsnTyr: 4.05 ± 0.342
0.0AsnXaa: 0.0 ± 0.0
Pro
0.903ProAla: 0.903 ± 0.147
0.146ProCys: 0.146 ± 0.076
1.439ProAsp: 1.439 ± 0.204
2.245ProGlu: 2.245 ± 0.259
1.22ProPhe: 1.22 ± 0.184
0.878ProGly: 0.878 ± 0.155
0.244ProHis: 0.244 ± 0.07
2.22ProIle: 2.22 ± 0.206
2.391ProLys: 2.391 ± 0.221
2.171ProLeu: 2.171 ± 0.181
0.512ProMet: 0.512 ± 0.121
1.927ProAsn: 1.927 ± 0.285
0.586ProPro: 0.586 ± 0.119
1.195ProGln: 1.195 ± 0.263
0.732ProArg: 0.732 ± 0.159
1.732ProSer: 1.732 ± 0.256
1.879ProThr: 1.879 ± 0.221
1.317ProVal: 1.317 ± 0.188
0.146ProTrp: 0.146 ± 0.053
1.366ProTyr: 1.366 ± 0.17
0.0ProXaa: 0.0 ± 0.0
Gln
1.708GlnAla: 1.708 ± 0.247
0.122GlnCys: 0.122 ± 0.056
2.171GlnAsp: 2.171 ± 0.295
2.928GlnGlu: 2.928 ± 0.37
1.147GlnPhe: 1.147 ± 0.138
2.196GlnGly: 2.196 ± 0.267
0.61GlnHis: 0.61 ± 0.132
2.171GlnIle: 2.171 ± 0.238
2.635GlnLys: 2.635 ± 0.288
2.684GlnLeu: 2.684 ± 0.27
0.732GlnMet: 0.732 ± 0.158
1.513GlnAsn: 1.513 ± 0.228
0.951GlnPro: 0.951 ± 0.303
2.415GlnGln: 2.415 ± 0.604
1.244GlnArg: 1.244 ± 0.187
1.927GlnSer: 1.927 ± 0.168
1.513GlnThr: 1.513 ± 0.202
2.415GlnVal: 2.415 ± 0.292
0.244GlnTrp: 0.244 ± 0.07
1.561GlnTyr: 1.561 ± 0.17
0.0GlnXaa: 0.0 ± 0.0
Arg
1.659ArgAla: 1.659 ± 0.219
0.22ArgCys: 0.22 ± 0.082
2.513ArgAsp: 2.513 ± 0.238
3.245ArgGlu: 3.245 ± 0.304
1.732ArgPhe: 1.732 ± 0.169
1.757ArgGly: 1.757 ± 0.286
0.61ArgHis: 0.61 ± 0.12
2.733ArgIle: 2.733 ± 0.257
2.976ArgLys: 2.976 ± 0.306
3.025ArgLeu: 3.025 ± 0.282
0.951ArgMet: 0.951 ± 0.15
2.342ArgAsn: 2.342 ± 0.252
1.195ArgPro: 1.195 ± 0.177
1.171ArgGln: 1.171 ± 0.199
1.927ArgArg: 1.927 ± 0.245
1.244ArgSer: 1.244 ± 0.155
1.805ArgThr: 1.805 ± 0.19
2.635ArgVal: 2.635 ± 0.307
0.317ArgTrp: 0.317 ± 0.104
2.196ArgTyr: 2.196 ± 0.219
0.0ArgXaa: 0.0 ± 0.0
Ser
2.44SerAla: 2.44 ± 0.253
0.268SerCys: 0.268 ± 0.083
4.196SerAsp: 4.196 ± 0.329
4.44SerGlu: 4.44 ± 0.361
2.854SerPhe: 2.854 ± 0.3
3.538SerGly: 3.538 ± 0.346
0.927SerHis: 0.927 ± 0.128
5.441SerIle: 5.441 ± 0.346
6.807SerLys: 6.807 ± 0.492
5.075SerLeu: 5.075 ± 0.353
1.342SerMet: 1.342 ± 0.203
4.709SerAsn: 4.709 ± 0.392
1.635SerPro: 1.635 ± 0.202
1.635SerGln: 1.635 ± 0.256
2.001SerArg: 2.001 ± 0.203
4.392SerSer: 4.392 ± 0.352
4.001SerThr: 4.001 ± 0.332
3.855SerVal: 3.855 ± 0.334
0.683SerTrp: 0.683 ± 0.124
3.684SerTyr: 3.684 ± 0.318
0.0SerXaa: 0.0 ± 0.0
Thr
1.927ThrAla: 1.927 ± 0.223
0.317ThrCys: 0.317 ± 0.083
3.464ThrAsp: 3.464 ± 0.327
4.514ThrGlu: 4.514 ± 0.39
2.635ThrPhe: 2.635 ± 0.226
3.196ThrGly: 3.196 ± 0.273
1.073ThrHis: 1.073 ± 0.156
5.026ThrIle: 5.026 ± 0.324
5.465ThrLys: 5.465 ± 0.38
5.294ThrLeu: 5.294 ± 0.389
0.854ThrMet: 0.854 ± 0.124
4.026ThrAsn: 4.026 ± 0.322
1.976ThrPro: 1.976 ± 0.201
2.537ThrGln: 2.537 ± 0.284
2.171ThrArg: 2.171 ± 0.255
3.586ThrSer: 3.586 ± 0.302
3.708ThrThr: 3.708 ± 0.308
4.27ThrVal: 4.27 ± 0.37
0.464ThrTrp: 0.464 ± 0.096
2.708ThrTyr: 2.708 ± 0.232
0.0ThrXaa: 0.0 ± 0.0
Val
2.415ValAla: 2.415 ± 0.257
0.415ValCys: 0.415 ± 0.105
4.172ValAsp: 4.172 ± 0.293
5.465ValGlu: 5.465 ± 0.436
2.293ValPhe: 2.293 ± 0.285
3.294ValGly: 3.294 ± 0.321
0.756ValHis: 0.756 ± 0.13
5.123ValIle: 5.123 ± 0.347
6.368ValLys: 6.368 ± 0.48
4.855ValLeu: 4.855 ± 0.399
1.317ValMet: 1.317 ± 0.175
4.44ValAsn: 4.44 ± 0.307
1.659ValPro: 1.659 ± 0.195
1.513ValGln: 1.513 ± 0.221
2.22ValArg: 2.22 ± 0.234
4.684ValSer: 4.684 ± 0.341
4.123ValThr: 4.123 ± 0.381
3.44ValVal: 3.44 ± 0.35
0.708ValTrp: 0.708 ± 0.16
3.147ValTyr: 3.147 ± 0.261
0.0ValXaa: 0.0 ± 0.0
Trp
0.293TrpAla: 0.293 ± 0.09
0.049TrpCys: 0.049 ± 0.031
0.586TrpAsp: 0.586 ± 0.111
0.683TrpGlu: 0.683 ± 0.116
0.488TrpPhe: 0.488 ± 0.104
0.439TrpGly: 0.439 ± 0.11
0.146TrpHis: 0.146 ± 0.06
0.537TrpIle: 0.537 ± 0.122
0.805TrpLys: 0.805 ± 0.149
0.439TrpLeu: 0.439 ± 0.105
0.122TrpMet: 0.122 ± 0.065
0.732TrpAsn: 0.732 ± 0.156
0.0TrpPro: 0.0 ± 0.0
0.244TrpGln: 0.244 ± 0.089
0.146TrpArg: 0.146 ± 0.058
0.488TrpSer: 0.488 ± 0.101
0.512TrpThr: 0.512 ± 0.124
0.586TrpVal: 0.586 ± 0.115
0.073TrpTrp: 0.073 ± 0.05
0.659TrpTyr: 0.659 ± 0.119
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.61TyrAla: 1.61 ± 0.222
0.488TyrCys: 0.488 ± 0.11
3.879TyrAsp: 3.879 ± 0.315
3.416TyrGlu: 3.416 ± 0.355
2.513TyrPhe: 2.513 ± 0.245
2.976TyrGly: 2.976 ± 0.315
0.903TyrHis: 0.903 ± 0.171
4.44TyrIle: 4.44 ± 0.413
5.221TyrLys: 5.221 ± 0.422
4.416TyrLeu: 4.416 ± 0.373
1.293TyrMet: 1.293 ± 0.188
4.318TyrAsn: 4.318 ± 0.315
1.391TyrPro: 1.391 ± 0.179
1.586TyrGln: 1.586 ± 0.199
1.83TyrArg: 1.83 ± 0.23
2.952TyrSer: 2.952 ± 0.299
3.294TyrThr: 3.294 ± 0.314
3.416TyrVal: 3.416 ± 0.326
0.293TyrTrp: 0.293 ± 0.078
2.952TyrTyr: 2.952 ± 0.265
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 203 proteins (40989 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski