Human immunodeficiency virus type 1 group M subtype B (strain 89.6) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group M; HIV-1 M:B

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q73370-2|TAT-2_HV1B9 Isoform of Q73370 Isoform Short of Protein Tat OS=Human immunodeficiency virus type 1 group M subtype B (strain 89.6) OX=401671 GN=tat PE=4 SV=1
MM1 pKa = 7.74LSLQILAIVALVVAAIIAIVVWSIVFIEE29 pKa = 3.94YY30 pKa = 10.41RR31 pKa = 11.84KK32 pKa = 9.66ILRR35 pKa = 11.84QRR37 pKa = 11.84KK38 pKa = 8.02IDD40 pKa = 3.69RR41 pKa = 11.84LIDD44 pKa = 4.31RR45 pKa = 11.84IRR47 pKa = 11.84EE48 pKa = 3.99RR49 pKa = 11.84EE50 pKa = 3.78EE51 pKa = 4.6DD52 pKa = 3.51SGNEE56 pKa = 3.94SEE58 pKa = 5.46GDD60 pKa = 3.79QEE62 pKa = 4.05EE63 pKa = 4.39LAALEE68 pKa = 4.57RR69 pKa = 11.84GHH71 pKa = 7.06LAPWDD76 pKa = 3.77VDD78 pKa = 3.88DD79 pKa = 5.48LL80 pKa = 4.6

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q89842|NEF_HV1B9 Protein Nef OS=Human immunodeficiency virus type 1 group M subtype B (strain 89.6) OX=401671 GN=nef PE=3 SV=3
MM1 pKa = 7.3EE2 pKa = 5.21NRR4 pKa = 11.84WQVMIVWQVDD14 pKa = 3.04RR15 pKa = 11.84MRR17 pKa = 11.84IRR19 pKa = 11.84TWKK22 pKa = 10.59SLVKK26 pKa = 9.59HH27 pKa = 6.07HH28 pKa = 6.5MYY30 pKa = 10.55ISGKK34 pKa = 9.99AKK36 pKa = 8.19GWSYY40 pKa = 9.29RR41 pKa = 11.84HH42 pKa = 6.44HH43 pKa = 6.86YY44 pKa = 10.29EE45 pKa = 3.75STNPRR50 pKa = 11.84ISSEE54 pKa = 3.9VHH56 pKa = 5.92IPLGDD61 pKa = 3.56AKK63 pKa = 10.91LVVTTYY69 pKa = 9.85WGLHH73 pKa = 4.29TGEE76 pKa = 4.83RR77 pKa = 11.84DD78 pKa = 2.76WHH80 pKa = 6.32LGQGVSIEE88 pKa = 4.03WRR90 pKa = 11.84KK91 pKa = 10.19KK92 pKa = 9.67RR93 pKa = 11.84YY94 pKa = 7.56STQVDD99 pKa = 3.34PGLADD104 pKa = 3.92RR105 pKa = 11.84LIHH108 pKa = 7.11LYY110 pKa = 10.95YY111 pKa = 10.3FDD113 pKa = 5.21CFSDD117 pKa = 3.48SAIRR121 pKa = 11.84KK122 pKa = 9.29SILGHH127 pKa = 5.81IVSPSCEE134 pKa = 3.9YY135 pKa = 10.44QAGHH139 pKa = 6.2NKK141 pKa = 9.66VGSLQYY147 pKa = 10.68LALAALTTPRR157 pKa = 11.84RR158 pKa = 11.84IKK160 pKa = 10.62PPFPSVTKK168 pKa = 9.6LTEE171 pKa = 4.23DD172 pKa = 3.49RR173 pKa = 11.84WNKK176 pKa = 7.33PQKK179 pKa = 9.13TKK181 pKa = 8.84GHH183 pKa = 6.54RR184 pKa = 11.84GSHH187 pKa = 4.99TMTGHH192 pKa = 6.71

Molecular weight: 22.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3647

71

1435

364.7

41.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.224 ± 0.473

2.002 ± 0.437

4.003 ± 0.407

6.965 ± 0.515

2.577 ± 0.153

7.239 ± 0.228

2.194 ± 0.481

6.307 ± 0.507

7.102 ± 1.05

8.226 ± 0.58

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.111 ± 0.203

4.661 ± 0.859

5.292 ± 0.567

5.895 ± 0.499

6.252 ± 1.02

5.539 ± 0.454

6.005 ± 0.466

6.197 ± 0.425

2.66 ± 0.222

2.55 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski