Amino acid dipepetide frequency for Pontibacter sp. 2b14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.842AlaAla: 7.842 ± 0.113
0.675AlaCys: 0.675 ± 0.026
4.289AlaAsp: 4.289 ± 0.073
5.235AlaGlu: 5.235 ± 0.082
3.76AlaPhe: 3.76 ± 0.059
6.369AlaGly: 6.369 ± 0.095
1.434AlaHis: 1.434 ± 0.041
5.367AlaIle: 5.367 ± 0.08
4.782AlaLys: 4.782 ± 0.073
7.933AlaLeu: 7.933 ± 0.113
1.971AlaMet: 1.971 ± 0.048
3.644AlaAsn: 3.644 ± 0.065
2.941AlaPro: 2.941 ± 0.065
3.512AlaGln: 3.512 ± 0.066
3.195AlaArg: 3.195 ± 0.059
5.024AlaSer: 5.024 ± 0.083
5.148AlaThr: 5.148 ± 0.099
5.39AlaVal: 5.39 ± 0.084
0.754AlaTrp: 0.754 ± 0.028
3.048AlaTyr: 3.048 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.525CysAla: 0.525 ± 0.025
0.107CysCys: 0.107 ± 0.01
0.365CysAsp: 0.365 ± 0.019
0.423CysGlu: 0.423 ± 0.023
0.344CysPhe: 0.344 ± 0.018
0.603CysGly: 0.603 ± 0.029
0.18CysHis: 0.18 ± 0.014
0.467CysIle: 0.467 ± 0.023
0.374CysLys: 0.374 ± 0.02
0.653CysLeu: 0.653 ± 0.023
0.151CysMet: 0.151 ± 0.013
0.327CysAsn: 0.327 ± 0.017
0.34CysPro: 0.34 ± 0.022
0.259CysGln: 0.259 ± 0.016
0.316CysArg: 0.316 ± 0.021
0.503CysSer: 0.503 ± 0.023
0.433CysThr: 0.433 ± 0.022
0.41CysVal: 0.41 ± 0.021
0.063CysTrp: 0.063 ± 0.008
0.269CysTyr: 0.269 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
4.162AspAla: 4.162 ± 0.066
0.34AspCys: 0.34 ± 0.02
2.186AspAsp: 2.186 ± 0.058
3.411AspGlu: 3.411 ± 0.061
2.944AspPhe: 2.944 ± 0.063
3.311AspGly: 3.311 ± 0.074
0.872AspHis: 0.872 ± 0.034
3.497AspIle: 3.497 ± 0.057
3.619AspLys: 3.619 ± 0.061
5.004AspLeu: 5.004 ± 0.076
1.224AspMet: 1.224 ± 0.033
2.569AspAsn: 2.569 ± 0.055
1.926AspPro: 1.926 ± 0.039
1.758AspGln: 1.758 ± 0.045
2.125AspArg: 2.125 ± 0.045
2.594AspSer: 2.594 ± 0.057
2.693AspThr: 2.693 ± 0.048
3.436AspVal: 3.436 ± 0.053
0.654AspTrp: 0.654 ± 0.024
2.262AspTyr: 2.262 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
5.661GluAla: 5.661 ± 0.084
0.289GluCys: 0.289 ± 0.019
2.976GluAsp: 2.976 ± 0.054
4.705GluGlu: 4.705 ± 0.084
2.405GluPhe: 2.405 ± 0.055
3.918GluGly: 3.918 ± 0.068
1.224GluHis: 1.224 ± 0.033
4.192GluIle: 4.192 ± 0.069
4.62GluLys: 4.62 ± 0.076
6.74GluLeu: 6.74 ± 0.091
1.628GluMet: 1.628 ± 0.038
3.436GluAsn: 3.436 ± 0.049
2.011GluPro: 2.011 ± 0.047
3.254GluGln: 3.254 ± 0.069
3.04GluArg: 3.04 ± 0.054
3.096GluSer: 3.096 ± 0.055
3.385GluThr: 3.385 ± 0.062
4.674GluVal: 4.674 ± 0.072
0.586GluTrp: 0.586 ± 0.026
1.982GluTyr: 1.982 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.46PheAla: 3.46 ± 0.055
0.396PheCys: 0.396 ± 0.019
2.606PheAsp: 2.606 ± 0.051
2.888PheGlu: 2.888 ± 0.05
2.208PhePhe: 2.208 ± 0.05
3.315PheGly: 3.315 ± 0.062
0.693PheHis: 0.693 ± 0.023
3.12PheIle: 3.12 ± 0.068
2.737PheLys: 2.737 ± 0.048
4.265PheLeu: 4.265 ± 0.073
1.127PheMet: 1.127 ± 0.034
2.38PheAsn: 2.38 ± 0.056
1.576PhePro: 1.576 ± 0.039
1.312PheGln: 1.312 ± 0.04
2.034PheArg: 2.034 ± 0.043
3.302PheSer: 3.302 ± 0.059
3.007PheThr: 3.007 ± 0.053
3.02PheVal: 3.02 ± 0.057
0.54PheTrp: 0.54 ± 0.027
1.874PheTyr: 1.874 ± 0.043
0.001PheXaa: 0.001 ± 0.001
Gly
5.075GlyAla: 5.075 ± 0.09
0.629GlyCys: 0.629 ± 0.028
3.142GlyAsp: 3.142 ± 0.067
3.616GlyGlu: 3.616 ± 0.062
3.489GlyPhe: 3.489 ± 0.064
5.059GlyGly: 5.059 ± 0.107
1.271GlyHis: 1.271 ± 0.035
5.095GlyIle: 5.095 ± 0.08
4.898GlyLys: 4.898 ± 0.077
6.318GlyLeu: 6.318 ± 0.083
1.759GlyMet: 1.759 ± 0.044
3.568GlyAsn: 3.568 ± 0.079
1.749GlyPro: 1.749 ± 0.042
2.556GlyGln: 2.556 ± 0.058
2.734GlyArg: 2.734 ± 0.061
4.349GlySer: 4.349 ± 0.095
4.439GlyThr: 4.439 ± 0.089
4.345GlyVal: 4.345 ± 0.076
0.753GlyTrp: 0.753 ± 0.031
2.856GlyTyr: 2.856 ± 0.062
0.0GlyXaa: 0.0 ± 0.0
His
1.33HisAla: 1.33 ± 0.039
0.155HisCys: 0.155 ± 0.011
0.875HisAsp: 0.875 ± 0.029
1.122HisGlu: 1.122 ± 0.034
1.054HisPhe: 1.054 ± 0.035
1.2HisGly: 1.2 ± 0.036
0.557HisHis: 0.557 ± 0.022
1.354HisIle: 1.354 ± 0.036
0.99HisLys: 0.99 ± 0.031
1.913HisLeu: 1.913 ± 0.045
0.437HisMet: 0.437 ± 0.019
0.917HisAsn: 0.917 ± 0.031
1.038HisPro: 1.038 ± 0.029
0.825HisGln: 0.825 ± 0.03
0.84HisArg: 0.84 ± 0.027
0.982HisSer: 0.982 ± 0.031
1.307HisThr: 1.307 ± 0.037
1.127HisVal: 1.127 ± 0.03
0.198HisTrp: 0.198 ± 0.015
0.838HisTyr: 0.838 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.46IleAla: 5.46 ± 0.079
0.554IleCys: 0.554 ± 0.026
3.403IleAsp: 3.403 ± 0.057
4.056IleGlu: 4.056 ± 0.069
2.667IlePhe: 2.667 ± 0.055
4.634IleGly: 4.634 ± 0.074
1.077IleHis: 1.077 ± 0.031
4.248IleIle: 4.248 ± 0.071
4.166IleLys: 4.166 ± 0.065
6.694IleLeu: 6.694 ± 0.089
1.26IleMet: 1.26 ± 0.038
3.395IleAsn: 3.395 ± 0.068
2.841IlePro: 2.841 ± 0.061
2.265IleGln: 2.265 ± 0.046
3.053IleArg: 3.053 ± 0.053
4.405IleSer: 4.405 ± 0.08
4.36IleThr: 4.36 ± 0.07
3.95IleVal: 3.95 ± 0.07
0.578IleTrp: 0.578 ± 0.023
2.2IleTyr: 2.2 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
5.474LysAla: 5.474 ± 0.082
0.266LysCys: 0.266 ± 0.016
3.309LysAsp: 3.309 ± 0.061
4.461LysGlu: 4.461 ± 0.081
2.089LysPhe: 2.089 ± 0.049
3.999LysGly: 3.999 ± 0.066
1.203LysHis: 1.203 ± 0.035
3.884LysIle: 3.884 ± 0.057
4.436LysLys: 4.436 ± 0.08
6.34LysLeu: 6.34 ± 0.08
1.657LysMet: 1.657 ± 0.043
3.126LysAsn: 3.126 ± 0.064
2.798LysPro: 2.798 ± 0.05
2.943LysGln: 2.943 ± 0.064
2.725LysArg: 2.725 ± 0.046
3.442LysSer: 3.442 ± 0.057
3.711LysThr: 3.711 ± 0.061
4.44LysVal: 4.44 ± 0.07
0.589LysTrp: 0.589 ± 0.025
2.854LysTyr: 2.854 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
8.318LeuAla: 8.318 ± 0.116
0.734LeuCys: 0.734 ± 0.026
4.987LeuAsp: 4.987 ± 0.071
6.055LeuGlu: 6.055 ± 0.096
4.376LeuPhe: 4.376 ± 0.082
6.031LeuGly: 6.031 ± 0.099
2.104LeuHis: 2.104 ± 0.048
5.807LeuIle: 5.807 ± 0.095
6.442LeuLys: 6.442 ± 0.096
10.817LeuLeu: 10.817 ± 0.164
2.236LeuMet: 2.236 ± 0.053
5.041LeuAsn: 5.041 ± 0.083
4.572LeuPro: 4.572 ± 0.076
4.961LeuGln: 4.961 ± 0.088
4.398LeuArg: 4.398 ± 0.068
6.396LeuSer: 6.396 ± 0.088
5.955LeuThr: 5.955 ± 0.087
6.287LeuVal: 6.287 ± 0.097
0.86LeuTrp: 0.86 ± 0.032
3.617LeuTyr: 3.617 ± 0.066
0.0LeuXaa: 0.0 ± 0.0
Met
2.231MetAla: 2.231 ± 0.054
0.114MetCys: 0.114 ± 0.01
1.117MetAsp: 1.117 ± 0.032
1.419MetGlu: 1.419 ± 0.038
0.844MetPhe: 0.844 ± 0.025
1.552MetGly: 1.552 ± 0.041
0.517MetHis: 0.517 ± 0.023
1.297MetIle: 1.297 ± 0.039
1.728MetLys: 1.728 ± 0.041
2.591MetLeu: 2.591 ± 0.055
0.591MetMet: 0.591 ± 0.028
1.093MetAsn: 1.093 ± 0.032
1.195MetPro: 1.195 ± 0.037
1.26MetGln: 1.26 ± 0.035
1.141MetArg: 1.141 ± 0.039
1.287MetSer: 1.287 ± 0.034
1.037MetThr: 1.037 ± 0.038
1.588MetVal: 1.588 ± 0.044
0.175MetTrp: 0.175 ± 0.012
0.651MetTyr: 0.651 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
3.838AsnAla: 3.838 ± 0.066
0.337AsnCys: 0.337 ± 0.021
2.257AsnAsp: 2.257 ± 0.048
2.857AsnGlu: 2.857 ± 0.046
2.304AsnPhe: 2.304 ± 0.051
3.493AsnGly: 3.493 ± 0.079
0.874AsnHis: 0.874 ± 0.028
3.348AsnIle: 3.348 ± 0.062
3.03AsnLys: 3.03 ± 0.061
5.025AsnLeu: 5.025 ± 0.081
1.196AsnMet: 1.196 ± 0.034
2.663AsnAsn: 2.663 ± 0.064
2.634AsnPro: 2.634 ± 0.055
2.025AsnGln: 2.025 ± 0.041
2.456AsnArg: 2.456 ± 0.055
2.941AsnSer: 2.941 ± 0.058
3.035AsnThr: 3.035 ± 0.057
2.993AsnVal: 2.993 ± 0.063
0.737AsnTrp: 0.737 ± 0.033
2.237AsnTyr: 2.237 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
3.868ProAla: 3.868 ± 0.078
0.224ProCys: 0.224 ± 0.015
2.72ProAsp: 2.72 ± 0.061
3.505ProGlu: 3.505 ± 0.057
1.812ProPhe: 1.812 ± 0.046
2.635ProGly: 2.635 ± 0.058
0.775ProHis: 0.775 ± 0.028
2.248ProIle: 2.248 ± 0.047
2.172ProLys: 2.172 ± 0.045
3.514ProLeu: 3.514 ± 0.063
0.766ProMet: 0.766 ± 0.028
1.898ProAsn: 1.898 ± 0.051
1.005ProPro: 1.005 ± 0.036
1.536ProGln: 1.536 ± 0.041
1.283ProArg: 1.283 ± 0.034
2.102ProSer: 2.102 ± 0.049
2.204ProThr: 2.204 ± 0.048
3.458ProVal: 3.458 ± 0.066
0.365ProTrp: 0.365 ± 0.018
1.653ProTyr: 1.653 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
3.466GlnAla: 3.466 ± 0.053
0.175GlnCys: 0.175 ± 0.013
1.981GlnAsp: 1.981 ± 0.043
3.072GlnGlu: 3.072 ± 0.07
1.638GlnPhe: 1.638 ± 0.041
2.3GlnGly: 2.3 ± 0.061
1.11GlnHis: 1.11 ± 0.036
2.348GlnIle: 2.348 ± 0.049
2.72GlnLys: 2.72 ± 0.059
4.736GlnLeu: 4.736 ± 0.091
0.906GlnMet: 0.906 ± 0.03
2.236GlnAsn: 2.236 ± 0.053
1.827GlnPro: 1.827 ± 0.048
2.923GlnGln: 2.923 ± 0.081
1.898GlnArg: 1.898 ± 0.044
2.104GlnSer: 2.104 ± 0.046
2.312GlnThr: 2.312 ± 0.058
3.015GlnVal: 3.015 ± 0.052
0.35GlnTrp: 0.35 ± 0.02
1.361GlnTyr: 1.361 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.861ArgAla: 2.861 ± 0.058
0.272ArgCys: 0.272 ± 0.017
2.089ArgAsp: 2.089 ± 0.049
2.864ArgGlu: 2.864 ± 0.055
2.184ArgPhe: 2.184 ± 0.051
2.383ArgGly: 2.383 ± 0.053
0.878ArgHis: 0.878 ± 0.036
3.189ArgIle: 3.189 ± 0.057
2.916ArgLys: 2.916 ± 0.057
4.263ArgLeu: 4.263 ± 0.075
1.216ArgMet: 1.216 ± 0.032
2.504ArgAsn: 2.504 ± 0.048
1.536ArgPro: 1.536 ± 0.046
1.901ArgGln: 1.901 ± 0.044
1.97ArgArg: 1.97 ± 0.061
2.426ArgSer: 2.426 ± 0.051
2.479ArgThr: 2.479 ± 0.045
2.682ArgVal: 2.682 ± 0.056
0.49ArgTrp: 0.49 ± 0.024
1.83ArgTyr: 1.83 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
4.376SerAla: 4.376 ± 0.076
0.524SerCys: 0.524 ± 0.025
3.075SerAsp: 3.075 ± 0.059
3.721SerGlu: 3.721 ± 0.06
3.193SerPhe: 3.193 ± 0.053
4.705SerGly: 4.705 ± 0.097
1.091SerHis: 1.091 ± 0.036
4.567SerIle: 4.567 ± 0.077
3.559SerLys: 3.559 ± 0.066
5.526SerLeu: 5.526 ± 0.081
1.508SerMet: 1.508 ± 0.041
2.778SerAsn: 2.778 ± 0.063
2.198SerPro: 2.198 ± 0.05
2.149SerGln: 2.149 ± 0.053
2.478SerArg: 2.478 ± 0.054
3.784SerSer: 3.784 ± 0.079
3.334SerThr: 3.334 ± 0.072
3.901SerVal: 3.901 ± 0.067
0.63SerTrp: 0.63 ± 0.028
2.536SerTyr: 2.536 ± 0.059
0.0SerXaa: 0.0 ± 0.0
Thr
5.136ThrAla: 5.136 ± 0.085
0.372ThrCys: 0.372 ± 0.02
3.407ThrAsp: 3.407 ± 0.057
3.681ThrGlu: 3.681 ± 0.056
2.921ThrPhe: 2.921 ± 0.06
4.742ThrGly: 4.742 ± 0.101
1.037ThrHis: 1.037 ± 0.03
4.14ThrIle: 4.14 ± 0.075
3.141ThrLys: 3.141 ± 0.048
5.919ThrLeu: 5.919 ± 0.087
1.08ThrMet: 1.08 ± 0.035
2.664ThrAsn: 2.664 ± 0.069
2.913ThrPro: 2.913 ± 0.051
2.129ThrGln: 2.129 ± 0.048
2.183ThrArg: 2.183 ± 0.047
3.798ThrSer: 3.798 ± 0.079
3.863ThrThr: 3.863 ± 0.095
4.107ThrVal: 4.107 ± 0.08
0.635ThrTrp: 0.635 ± 0.028
2.463ThrTyr: 2.463 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
5.753ValAla: 5.753 ± 0.085
0.56ValCys: 0.56 ± 0.027
3.163ValAsp: 3.163 ± 0.06
3.916ValGlu: 3.916 ± 0.061
2.923ValPhe: 2.923 ± 0.059
4.146ValGly: 4.146 ± 0.074
1.141ValHis: 1.141 ± 0.032
4.293ValIle: 4.293 ± 0.077
4.144ValLys: 4.144 ± 0.064
6.68ValLeu: 6.68 ± 0.084
1.634ValMet: 1.634 ± 0.04
3.204ValAsn: 3.204 ± 0.057
2.785ValPro: 2.785 ± 0.054
2.796ValGln: 2.796 ± 0.051
2.726ValArg: 2.726 ± 0.052
4.203ValSer: 4.203 ± 0.07
4.208ValThr: 4.208 ± 0.09
4.627ValVal: 4.627 ± 0.079
0.711ValTrp: 0.711 ± 0.025
2.641ValTyr: 2.641 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.786TrpAla: 0.786 ± 0.029
0.093TrpCys: 0.093 ± 0.009
0.539TrpAsp: 0.539 ± 0.022
0.644TrpGlu: 0.644 ± 0.029
0.493TrpPhe: 0.493 ± 0.022
0.609TrpGly: 0.609 ± 0.026
0.238TrpHis: 0.238 ± 0.015
0.575TrpIle: 0.575 ± 0.024
0.611TrpLys: 0.611 ± 0.025
1.169TrpLeu: 1.169 ± 0.033
0.304TrpMet: 0.304 ± 0.017
0.53TrpAsn: 0.53 ± 0.024
0.322TrpPro: 0.322 ± 0.016
0.561TrpGln: 0.561 ± 0.023
0.5TrpArg: 0.5 ± 0.022
0.537TrpSer: 0.537 ± 0.025
0.493TrpThr: 0.493 ± 0.024
0.686TrpVal: 0.686 ± 0.025
0.14TrpTrp: 0.14 ± 0.011
0.388TrpTyr: 0.388 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.692TyrAla: 2.692 ± 0.056
0.299TyrCys: 0.299 ± 0.018
2.099TyrAsp: 2.099 ± 0.052
2.169TyrGlu: 2.169 ± 0.044
2.211TyrPhe: 2.211 ± 0.055
2.696TyrGly: 2.696 ± 0.068
0.785TyrHis: 0.785 ± 0.029
2.229TyrIle: 2.229 ± 0.044
2.659TyrLys: 2.659 ± 0.045
3.894TyrLeu: 3.894 ± 0.064
0.775TyrMet: 0.775 ± 0.029
2.31TyrAsn: 2.31 ± 0.049
1.535TyrPro: 1.535 ± 0.037
1.552TyrGln: 1.552 ± 0.036
1.838TyrArg: 1.838 ± 0.047
2.36TyrSer: 2.36 ± 0.047
2.929TyrThr: 2.929 ± 0.06
2.116TyrVal: 2.116 ± 0.045
0.411TyrTrp: 0.411 ± 0.019
1.685TyrTyr: 1.685 ± 0.049
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.001XaaVal: 0.001 ± 0.001
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3170 proteins (1063188 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski