Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis; Arabidopsis lyrata

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7LQW4|D7LQW4_ARALL Expressed protein OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_904767 PE=4 SV=1
MM1 pKa = 7.43AVFVTSPDD9 pKa = 3.44EE10 pKa = 4.33NSDD13 pKa = 3.49VVYY16 pKa = 10.67SFSGYY21 pKa = 10.28SPASEE26 pKa = 5.16IVDD29 pKa = 3.79CYY31 pKa = 11.51LNGKK35 pKa = 8.97SPPKK39 pKa = 10.29LINSQSKK46 pKa = 10.62LGFWWEE52 pKa = 4.05DD53 pKa = 2.97PDD55 pKa = 4.75LYY57 pKa = 10.63RR58 pKa = 11.84YY59 pKa = 10.34CDD61 pKa = 4.52DD62 pKa = 4.42LSEE65 pKa = 4.97LNTIEE70 pKa = 3.97DD71 pKa = 2.99RR72 pKa = 11.84MMRR75 pKa = 11.84TRR77 pKa = 11.84KK78 pKa = 9.74HH79 pKa = 7.17LMDD82 pKa = 4.71CLEE85 pKa = 4.68KK86 pKa = 10.57KK87 pKa = 9.97EE88 pKa = 4.08KK89 pKa = 10.46SQFVSNSDD97 pKa = 3.42QNPNTDD103 pKa = 3.57EE104 pKa = 3.96IFNNGGSSSSSSQIASDD121 pKa = 4.19LGQNPSTSSPSSSQIVSFDD140 pKa = 3.52QNSYY144 pKa = 9.4TSLGKK149 pKa = 10.62LCGEE153 pKa = 4.6PSSQVTCYY161 pKa = 10.49DD162 pKa = 3.3QNPNFSVGEE171 pKa = 3.91YY172 pKa = 10.45SCDD175 pKa = 3.19QSRR178 pKa = 11.84YY179 pKa = 9.73LVNEE183 pKa = 3.89DD184 pKa = 4.19PGFVDD189 pKa = 4.8CLCEE193 pKa = 4.17TEE195 pKa = 4.36EE196 pKa = 4.43EE197 pKa = 4.37NNGMSLPQEE206 pKa = 4.25TQTPSLFTEE215 pKa = 4.7DD216 pKa = 3.17QSFWEE221 pKa = 4.22NLFKK225 pKa = 11.38DD226 pKa = 3.62EE227 pKa = 5.33DD228 pKa = 4.03NVFGLLNDD236 pKa = 3.69NLEE239 pKa = 4.36VPLQDD244 pKa = 3.37HH245 pKa = 6.61SSTNEE250 pKa = 3.72EE251 pKa = 3.78EE252 pKa = 4.32DD253 pKa = 3.38EE254 pKa = 4.26FMIDD258 pKa = 2.64ISEE261 pKa = 4.2FLSEE265 pKa = 4.25EE266 pKa = 4.52EE267 pKa = 3.99EE268 pKa = 4.53FEE270 pKa = 4.19WPLFF274 pKa = 3.95

Molecular weight: 31.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7MRS9|D7MRS9_ARALL Copper transporter OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_496052 PE=3 SV=1
LL1 pKa = 7.35HH2 pKa = 7.11LRR4 pKa = 11.84QLRR7 pKa = 11.84ILHH10 pKa = 5.79QNNIHH15 pKa = 5.95RR16 pKa = 11.84RR17 pKa = 11.84SRR19 pKa = 11.84SILRR23 pKa = 11.84QNNIHH28 pKa = 6.17RR29 pKa = 11.84RR30 pKa = 11.84SRR32 pKa = 11.84SILRR36 pKa = 11.84QNNIHH41 pKa = 6.04RR42 pKa = 11.84RR43 pKa = 11.84SRR45 pKa = 11.84STHH48 pKa = 4.61RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84TRR53 pKa = 11.84LTLIHH58 pKa = 6.67RR59 pKa = 11.84RR60 pKa = 11.84SKK62 pKa = 9.9LTLIRR67 pKa = 11.84RR68 pKa = 3.92

Molecular weight: 8.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32097

16

32113

11683288

49

5090

363.8

40.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.294 ± 0.014

1.868 ± 0.008

5.37 ± 0.008

6.674 ± 0.016

4.349 ± 0.009

6.383 ± 0.015

2.261 ± 0.007

5.382 ± 0.011

6.357 ± 0.015

9.566 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.005

4.389 ± 0.009

4.796 ± 0.013

3.445 ± 0.01

5.446 ± 0.01

9.064 ± 0.017

5.083 ± 0.008

6.691 ± 0.01

1.275 ± 0.005

2.838 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski