Amino acid dipepetide frequency for Clostridiales bacterium 1_7_47FAA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.819AlaAla: 8.819 ± 0.107	1.384AlaCys: 1.384 ± 0.029	4.711AlaAsp: 4.711 ± 0.053	4.787AlaGlu: 4.787 ± 0.057	3.22AlaPhe: 3.22 ± 0.049	8.023AlaGly: 8.023 ± 0.082	1.167AlaHis: 1.167 ± 0.02	4.943AlaIle: 4.943 ± 0.063	3.686AlaLys: 3.686 ± 0.053	7.278AlaLeu: 7.278 ± 0.066	2.861AlaMet: 2.861 ± 0.043	2.195AlaAsn: 2.195 ± 0.035	2.406AlaPro: 2.406 ± 0.042	2.483AlaGln: 2.483 ± 0.04	3.801AlaArg: 3.801 ± 0.046	4.398AlaSer: 4.398 ± 0.051	2.981AlaThr: 2.981 ± 0.05	7.107AlaVal: 7.107 ± 0.065	0.731AlaTrp: 0.731 ± 0.022	2.852AlaTyr: 2.852 ± 0.037	0.0AlaXaa: 0.0 ± 0.0
Cys	1.166CysAla: 1.166 ± 0.026	0.364CysCys: 0.364 ± 0.016	0.794CysAsp: 0.794 ± 0.022	0.774CysGlu: 0.774 ± 0.018	0.733CysPhe: 0.733 ± 0.022	1.72CysGly: 1.72 ± 0.028	0.38CysHis: 0.38 ± 0.015	1.186CysIle: 1.186 ± 0.026	0.61CysLys: 0.61 ± 0.018	1.438CysLeu: 1.438 ± 0.028	0.561CysMet: 0.561 ± 0.019	0.576CysAsn: 0.576 ± 0.018	0.776CysPro: 0.776 ± 0.022	0.499CysGln: 0.499 ± 0.015	1.022CysArg: 1.022 ± 0.026	1.046CysSer: 1.046 ± 0.026	0.774CysThr: 0.774 ± 0.02	1.113CysVal: 1.113 ± 0.026	0.154CysTrp: 0.154 ± 0.009	0.588CysTyr: 0.588 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.138AspAla: 4.138 ± 0.054	0.873AspCys: 0.873 ± 0.02	2.732AspAsp: 2.732 ± 0.052	4.009AspGlu: 4.009 ± 0.046	2.482AspPhe: 2.482 ± 0.039	5.379AspGly: 5.379 ± 0.068	0.909AspHis: 0.909 ± 0.02	4.539AspIle: 4.539 ± 0.052	3.134AspLys: 3.134 ± 0.044	4.283AspLeu: 4.283 ± 0.047	2.282AspMet: 2.282 ± 0.038	2.085AspAsn: 2.085 ± 0.034	1.756AspPro: 1.756 ± 0.029	1.468AspGln: 1.468 ± 0.028	3.077AspArg: 3.077 ± 0.042	3.25AspSer: 3.25 ± 0.043	3.076AspThr: 3.076 ± 0.04	3.771AspVal: 3.771 ± 0.052	0.68AspTrp: 0.68 ± 0.018	2.634AspTyr: 2.634 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	5.717GluAla: 5.717 ± 0.063	0.859GluCys: 0.859 ± 0.024	4.273GluAsp: 4.273 ± 0.047	6.151GluGlu: 6.151 ± 0.077	2.308GluPhe: 2.308 ± 0.038	4.921GluGly: 4.921 ± 0.059	1.39GluHis: 1.39 ± 0.031	4.489GluIle: 4.489 ± 0.052	4.699GluLys: 4.699 ± 0.059	6.195GluLeu: 6.195 ± 0.064	2.343GluMet: 2.343 ± 0.038	3.062GluAsn: 3.062 ± 0.041	2.08GluPro: 2.08 ± 0.038	2.741GluGln: 2.741 ± 0.041	3.46GluArg: 3.46 ± 0.051	3.139GluSer: 3.139 ± 0.043	3.394GluThr: 3.394 ± 0.047	3.886GluVal: 3.886 ± 0.055	0.659GluTrp: 0.659 ± 0.021	2.779GluTyr: 2.779 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	2.935PheAla: 2.935 ± 0.041	0.785PheCys: 0.785 ± 0.017	2.313PheAsp: 2.313 ± 0.035	2.311PheGlu: 2.311 ± 0.036	1.758PhePhe: 1.758 ± 0.033	3.185PheGly: 3.185 ± 0.053	0.881PheHis: 0.881 ± 0.023	2.789PheIle: 2.789 ± 0.048	1.895PheLys: 1.895 ± 0.034	3.975PheLeu: 3.975 ± 0.057	1.338PheMet: 1.338 ± 0.029	1.477PheAsn: 1.477 ± 0.026	1.529PhePro: 1.529 ± 0.03	1.174PheGln: 1.174 ± 0.025	1.812PheArg: 1.812 ± 0.034	2.755PheSer: 2.755 ± 0.035	2.372PheThr: 2.372 ± 0.035	2.681PheVal: 2.681 ± 0.041	0.439PheTrp: 0.439 ± 0.015	1.745PheTyr: 1.745 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	6.073GlyAla: 6.073 ± 0.072	1.56GlyCys: 1.56 ± 0.032	3.838GlyAsp: 3.838 ± 0.053	4.784GlyGlu: 4.784 ± 0.049	3.56GlyPhe: 3.56 ± 0.051	6.176GlyGly: 6.176 ± 0.073	1.555GlyHis: 1.555 ± 0.033	6.943GlyIle: 6.943 ± 0.069	4.916GlyLys: 4.916 ± 0.05	7.133GlyLeu: 7.133 ± 0.071	3.178GlyMet: 3.178 ± 0.046	3.566GlyAsn: 3.566 ± 0.053	2.013GlyPro: 2.013 ± 0.047	2.858GlyGln: 2.858 ± 0.051	4.475GlyArg: 4.475 ± 0.059	4.765GlySer: 4.765 ± 0.066	4.901GlyThr: 4.901 ± 0.061	5.362GlyVal: 5.362 ± 0.07	0.976GlyTrp: 0.976 ± 0.025	3.56GlyTyr: 3.56 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	1.208HisAla: 1.208 ± 0.024	0.32HisCys: 0.32 ± 0.013	0.938HisAsp: 0.938 ± 0.023	1.064HisGlu: 1.064 ± 0.027	0.859HisPhe: 0.859 ± 0.021	1.567HisGly: 1.567 ± 0.032	0.402HisHis: 0.402 ± 0.015	1.411HisIle: 1.411 ± 0.028	0.916HisLys: 0.916 ± 0.022	1.608HisLeu: 1.608 ± 0.034	0.746HisMet: 0.746 ± 0.021	0.659HisAsn: 0.659 ± 0.022	0.99HisPro: 0.99 ± 0.023	0.564HisGln: 0.564 ± 0.016	0.871HisArg: 0.871 ± 0.024	0.969HisSer: 0.969 ± 0.022	0.997HisThr: 0.997 ± 0.024	1.251HisVal: 1.251 ± 0.022	0.193HisTrp: 0.193 ± 0.01	0.766HisTyr: 0.766 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.398IleAla: 5.398 ± 0.064	1.318IleCys: 1.318 ± 0.027	3.487IleAsp: 3.487 ± 0.051	3.884IleGlu: 3.884 ± 0.047	2.699IlePhe: 2.699 ± 0.04	5.276IleGly: 5.276 ± 0.056	1.474IleHis: 1.474 ± 0.028	4.781IleIle: 4.781 ± 0.056	3.478IleLys: 3.478 ± 0.05	7.017IleLeu: 7.017 ± 0.076	2.174IleMet: 2.174 ± 0.033	2.609IleAsn: 2.609 ± 0.044	3.454IlePro: 3.454 ± 0.043	2.281IleGln: 2.281 ± 0.035	3.991IleArg: 3.991 ± 0.052	4.646IleSer: 4.646 ± 0.057	3.982IleThr: 3.982 ± 0.049	4.451IleVal: 4.451 ± 0.052	0.709IleTrp: 0.709 ± 0.02	2.584IleTyr: 2.584 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	4.844LysAla: 4.844 ± 0.063	0.596LysCys: 0.596 ± 0.02	3.631LysAsp: 3.631 ± 0.046	5.161LysGlu: 5.161 ± 0.059	1.356LysPhe: 1.356 ± 0.028	4.341LysGly: 4.341 ± 0.052	0.912LysHis: 0.912 ± 0.019	3.462LysIle: 3.462 ± 0.043	4.299LysLys: 4.299 ± 0.061	4.37LysLeu: 4.37 ± 0.054	1.875LysMet: 1.875 ± 0.031	2.451LysAsn: 2.451 ± 0.038	1.885LysPro: 1.885 ± 0.032	1.947LysGln: 1.947 ± 0.033	2.724LysArg: 2.724 ± 0.043	2.838LysSer: 2.838 ± 0.038	2.934LysThr: 2.934 ± 0.045	3.588LysVal: 3.588 ± 0.048	0.525LysTrp: 0.525 ± 0.016	2.028LysTyr: 2.028 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	7.349LeuAla: 7.349 ± 0.063	1.625LeuCys: 1.625 ± 0.036	5.294LeuAsp: 5.294 ± 0.053	6.262LeuGlu: 6.262 ± 0.069	3.846LeuPhe: 3.846 ± 0.051	6.703LeuGly: 6.703 ± 0.069	1.512LeuHis: 1.512 ± 0.025	5.707LeuIle: 5.707 ± 0.056	5.742LeuLys: 5.742 ± 0.065	8.456LeuLeu: 8.456 ± 0.096	2.997LeuMet: 2.997 ± 0.044	3.577LeuAsn: 3.577 ± 0.048	3.807LeuPro: 3.807 ± 0.047	2.454LeuGln: 2.454 ± 0.035	3.905LeuArg: 3.905 ± 0.053	6.151LeuSer: 6.151 ± 0.066	4.989LeuThr: 4.989 ± 0.059	5.832LeuVal: 5.832 ± 0.072	0.82LeuTrp: 0.82 ± 0.022	3.346LeuTyr: 3.346 ± 0.043	0.0LeuXaa: 0.0 ± 0.0
Met	3.103MetAla: 3.103 ± 0.045	0.431MetCys: 0.431 ± 0.015	2.55MetAsp: 2.55 ± 0.037	3.048MetGlu: 3.048 ± 0.045	1.229MetPhe: 1.229 ± 0.022	2.832MetGly: 2.832 ± 0.04	0.485MetHis: 0.485 ± 0.015	2.247MetIle: 2.247 ± 0.04	2.447MetLys: 2.447 ± 0.039	2.929MetLeu: 2.929 ± 0.045	1.209MetMet: 1.209 ± 0.024	1.547MetAsn: 1.547 ± 0.029	1.309MetPro: 1.309 ± 0.028	0.99MetGln: 0.99 ± 0.022	1.433MetArg: 1.433 ± 0.027	1.904MetSer: 1.904 ± 0.032	1.923MetThr: 1.923 ± 0.033	2.449MetVal: 2.449 ± 0.04	0.273MetTrp: 0.273 ± 0.011	0.961MetTyr: 0.961 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.81AsnAla: 2.81 ± 0.041	0.585AsnCys: 0.585 ± 0.019	1.784AsnAsp: 1.784 ± 0.03	2.199AsnGlu: 2.199 ± 0.041	1.339AsnPhe: 1.339 ± 0.028	3.318AsnGly: 3.318 ± 0.046	0.858AsnHis: 0.858 ± 0.02	2.905AsnIle: 2.905 ± 0.046	1.805AsnLys: 1.805 ± 0.028	3.469AsnLeu: 3.469 ± 0.043	1.359AsnMet: 1.359 ± 0.026	1.429AsnAsn: 1.429 ± 0.027	2.121AsnPro: 2.121 ± 0.035	1.58AsnGln: 1.58 ± 0.029	2.113AsnArg: 2.113 ± 0.034	2.045AsnSer: 2.045 ± 0.038	2.013AsnThr: 2.013 ± 0.03	2.526AsnVal: 2.526 ± 0.043	0.42AsnTrp: 0.42 ± 0.015	1.504AsnTyr: 1.504 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	2.993ProAla: 2.993 ± 0.043	0.58ProCys: 0.58 ± 0.022	2.735ProAsp: 2.735 ± 0.039	3.349ProGlu: 3.349 ± 0.054	1.629ProPhe: 1.629 ± 0.03	3.325ProGly: 3.325 ± 0.053	0.676ProHis: 0.676 ± 0.019	2.034ProIle: 2.034 ± 0.031	1.605ProLys: 1.605 ± 0.029	3.116ProLeu: 3.116 ± 0.042	1.142ProMet: 1.142 ± 0.025	1.098ProAsn: 1.098 ± 0.023	1.056ProPro: 1.056 ± 0.043	1.179ProGln: 1.179 ± 0.024	1.34ProArg: 1.34 ± 0.026	2.175ProSer: 2.175 ± 0.036	1.537ProThr: 1.537 ± 0.037	3.547ProVal: 3.547 ± 0.042	0.405ProTrp: 0.405 ± 0.016	1.618ProTyr: 1.618 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	2.974GlnAla: 2.974 ± 0.043	0.439GlnCys: 0.439 ± 0.018	1.83GlnAsp: 1.83 ± 0.035	2.682GlnGlu: 2.682 ± 0.045	1.155GlnPhe: 1.155 ± 0.025	2.526GlnGly: 2.526 ± 0.046	0.452GlnHis: 0.452 ± 0.014	2.284GlnIle: 2.284 ± 0.035	2.09GlnLys: 2.09 ± 0.036	2.721GlnLeu: 2.721 ± 0.04	1.306GlnMet: 1.306 ± 0.027	1.242GlnAsn: 1.242 ± 0.027	1.09GlnPro: 1.09 ± 0.024	1.064GlnGln: 1.064 ± 0.026	1.353GlnArg: 1.353 ± 0.029	1.628GlnSer: 1.628 ± 0.029	1.55GlnThr: 1.55 ± 0.028	2.402GlnVal: 2.402 ± 0.036	0.415GlnTrp: 0.415 ± 0.015	1.398GlnTyr: 1.398 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	3.103ArgAla: 3.103 ± 0.04	0.738ArgCys: 0.738 ± 0.021	2.671ArgAsp: 2.671 ± 0.044	4.154ArgGlu: 4.154 ± 0.055	2.123ArgPhe: 2.123 ± 0.036	3.009ArgGly: 3.009 ± 0.041	0.98ArgHis: 0.98 ± 0.024	3.839ArgIle: 3.839 ± 0.045	3.16ArgLys: 3.16 ± 0.039	4.746ArgLeu: 4.746 ± 0.053	1.984ArgMet: 1.984 ± 0.034	2.11ArgAsn: 2.11 ± 0.032	1.731ArgPro: 1.731 ± 0.032	2.095ArgGln: 2.095 ± 0.037	2.824ArgArg: 2.824 ± 0.043	2.42ArgSer: 2.42 ± 0.039	2.633ArgThr: 2.633 ± 0.041	2.848ArgVal: 2.848 ± 0.034	0.479ArgTrp: 0.479 ± 0.015	2.221ArgTyr: 2.221 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.261SerAla: 4.261 ± 0.05	0.947SerCys: 0.947 ± 0.021	2.967SerAsp: 2.967 ± 0.034	3.146SerGlu: 3.146 ± 0.043	2.546SerPhe: 2.546 ± 0.041	5.83SerGly: 5.83 ± 0.083	1.183SerHis: 1.183 ± 0.025	4.12SerIle: 4.12 ± 0.05	2.483SerLys: 2.483 ± 0.038	5.524SerLeu: 5.524 ± 0.059	2.162SerMet: 2.162 ± 0.032	1.976SerAsn: 1.976 ± 0.03	2.106SerPro: 2.106 ± 0.034	2.063SerGln: 2.063 ± 0.04	3.363SerArg: 3.363 ± 0.047	3.625SerSer: 3.625 ± 0.054	2.725SerThr: 2.725 ± 0.045	4.127SerVal: 4.127 ± 0.049	0.642SerTrp: 0.642 ± 0.022	2.296SerTyr: 2.296 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	4.783ThrAla: 4.783 ± 0.061	0.741ThrCys: 0.741 ± 0.022	3.063ThrAsp: 3.063 ± 0.041	3.201ThrGlu: 3.201 ± 0.04	2.016ThrPhe: 2.016 ± 0.033	5.379ThrGly: 5.379 ± 0.071	0.829ThrHis: 0.829 ± 0.021	3.528ThrIle: 3.528 ± 0.048	2.286ThrLys: 2.286 ± 0.038	4.583ThrLeu: 4.583 ± 0.046	1.646ThrMet: 1.646 ± 0.029	1.652ThrAsn: 1.652 ± 0.029	2.259ThrPro: 2.259 ± 0.034	1.462ThrGln: 1.462 ± 0.031	2.235ThrArg: 2.235 ± 0.034	2.915ThrSer: 2.915 ± 0.042	2.521ThrThr: 2.521 ± 0.041	4.325ThrVal: 4.325 ± 0.048	0.538ThrTrp: 0.538 ± 0.016	1.901ThrTyr: 1.901 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	4.858ValAla: 4.858 ± 0.057	1.287ValCys: 1.287 ± 0.028	3.867ValAsp: 3.867 ± 0.047	4.26ValGlu: 4.26 ± 0.05	3.091ValPhe: 3.091 ± 0.041	4.531ValGly: 4.531 ± 0.061	1.151ValHis: 1.151 ± 0.023	5.151ValIle: 5.151 ± 0.055	3.91ValLys: 3.91 ± 0.045	6.873ValLeu: 6.873 ± 0.065	2.431ValMet: 2.431 ± 0.039	2.771ValAsn: 2.771 ± 0.04	2.912ValPro: 2.912 ± 0.042	1.874ValGln: 1.874 ± 0.031	3.386ValArg: 3.386 ± 0.045	4.649ValSer: 4.649 ± 0.052	3.995ValThr: 3.995 ± 0.043	4.763ValVal: 4.763 ± 0.067	0.706ValTrp: 0.706 ± 0.016	2.715ValTyr: 2.715 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	0.616TrpAla: 0.616 ± 0.02	0.18TrpCys: 0.18 ± 0.01	0.676TrpAsp: 0.676 ± 0.018	0.72TrpGlu: 0.72 ± 0.019	0.455TrpPhe: 0.455 ± 0.017	0.781TrpGly: 0.781 ± 0.022	0.185TrpHis: 0.185 ± 0.009	0.696TrpIle: 0.696 ± 0.021	0.737TrpLys: 0.737 ± 0.021	0.943TrpLeu: 0.943 ± 0.022	0.404TrpMet: 0.404 ± 0.015	0.573TrpAsn: 0.573 ± 0.018	0.273TrpPro: 0.273 ± 0.011	0.394TrpGln: 0.394 ± 0.016	0.453TrpArg: 0.453 ± 0.015	0.525TrpSer: 0.525 ± 0.017	0.454TrpThr: 0.454 ± 0.017	0.6TrpVal: 0.6 ± 0.018	0.121TrpTrp: 0.121 ± 0.008	0.481TrpTyr: 0.481 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.687TyrAla: 2.687 ± 0.037	0.664TyrCys: 0.664 ± 0.02	2.407TyrAsp: 2.407 ± 0.037	2.6TyrGlu: 2.6 ± 0.038	1.746TyrPhe: 1.746 ± 0.034	3.369TyrGly: 3.369 ± 0.046	0.918TyrHis: 0.918 ± 0.023	2.636TyrIle: 2.636 ± 0.033	1.869TyrLys: 1.869 ± 0.028	3.715TyrLeu: 3.715 ± 0.047	1.297TyrMet: 1.297 ± 0.027	1.529TyrAsn: 1.529 ± 0.03	1.583TyrPro: 1.583 ± 0.033	1.43TyrGln: 1.43 ± 0.03	2.232TyrArg: 2.232 ± 0.041	2.249TyrSer: 2.249 ± 0.035	2.111TyrThr: 2.111 ± 0.03	2.553TyrVal: 2.553 ± 0.039	0.382TyrTrp: 0.382 ± 0.014	1.848TyrTyr: 1.848 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6251 proteins (1916964 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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