Tenacibaculum sp. SZ-18

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Tenacibaculum; unclassified Tenacibaculum

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8VDC7|A0A2K8VDC7_9FLAO ANK_REP_REGION domain-containing protein OS=Tenacibaculum sp. SZ-18 OX=754423 GN=BTO06_11215 PE=4 SV=1
MM1 pKa = 7.69LSLNIYY7 pKa = 9.06GQTIDD12 pKa = 4.14KK13 pKa = 9.67PILGFSSACGSDD25 pKa = 2.86GFNNFNFSFVFRR37 pKa = 11.84DD38 pKa = 3.82GLFDD42 pKa = 3.79TNNTFNVVISDD53 pKa = 3.66EE54 pKa = 4.4NGDD57 pKa = 3.75FGANPPVIFSITDD70 pKa = 3.38SNVNTFPTNPVDD82 pKa = 4.08GSFSVPDD89 pKa = 3.89DD90 pKa = 3.86AFGTSYY96 pKa = 10.53RR97 pKa = 11.84IKK99 pKa = 10.8VIANSPNIEE108 pKa = 4.57SPPSDD113 pKa = 3.8PFEE116 pKa = 4.14MYY118 pKa = 10.26RR119 pKa = 11.84ISSEE123 pKa = 3.85PLILNEE129 pKa = 4.44FNDD132 pKa = 5.44DD133 pKa = 3.81LVLCNGVAEE142 pKa = 4.43TVSLNLTDD150 pKa = 4.69PNVEE154 pKa = 4.6FIWYY158 pKa = 9.6RR159 pKa = 11.84NNTPITSQSSSSLTITQPGEE179 pKa = 3.88YY180 pKa = 9.28YY181 pKa = 11.11AEE183 pKa = 4.18INYY186 pKa = 9.13GGCPDD191 pKa = 3.97AVISNKK197 pKa = 10.0VIARR201 pKa = 11.84VVNSSSITIQGDD213 pKa = 3.52NTVEE217 pKa = 3.96LCADD221 pKa = 3.34QAYY224 pKa = 10.76DD225 pKa = 4.87LIASIDD231 pKa = 4.02DD232 pKa = 3.57PSFIYY237 pKa = 10.02KK238 pKa = 9.1WFKK241 pKa = 10.76DD242 pKa = 3.49GNEE245 pKa = 3.86ITGLPDD251 pKa = 3.04YY252 pKa = 10.31TPIYY256 pKa = 6.89TTPTSDD262 pKa = 3.01QFGVYY267 pKa = 9.6HH268 pKa = 6.71LEE270 pKa = 4.1IEE272 pKa = 4.27ISGCSSRR279 pKa = 11.84SQDD282 pKa = 2.95VTLEE286 pKa = 4.12QKK288 pKa = 10.57SDD290 pKa = 2.88AGFPVEE296 pKa = 4.31ISGNPTEE303 pKa = 4.56IVFPSEE309 pKa = 4.09EE310 pKa = 3.89FTLTVTHH317 pKa = 6.42EE318 pKa = 3.98ASGPVDD324 pKa = 3.51IKK326 pKa = 10.84WFDD329 pKa = 3.9ANGEE333 pKa = 4.28IPGATGDD340 pKa = 3.91TLNVSGPGEE349 pKa = 4.22YY350 pKa = 9.92YY351 pKa = 11.1AEE353 pKa = 4.24VTEE356 pKa = 4.86SGGDD360 pKa = 3.67CPVSVLSDD368 pKa = 2.82IRR370 pKa = 11.84EE371 pKa = 3.9ILGVKK376 pKa = 9.95EE377 pKa = 3.82MFVTIRR383 pKa = 11.84SGTDD387 pKa = 2.96YY388 pKa = 11.01DD389 pKa = 3.98EE390 pKa = 5.11CVSASTEE397 pKa = 3.83LTMVGISVIATDD409 pKa = 3.84DD410 pKa = 3.54NEE412 pKa = 4.42YY413 pKa = 11.07DD414 pKa = 3.61VSQEE418 pKa = 4.61KK419 pKa = 9.71IDD421 pKa = 4.25KK422 pKa = 10.31LLSPDD427 pKa = 3.16STGAIIMRR435 pKa = 11.84FQWYY439 pKa = 10.33KK440 pKa = 10.94NGTTITDD447 pKa = 3.68AEE449 pKa = 4.57SILYY453 pKa = 9.21NVNSYY458 pKa = 11.36AEE460 pKa = 3.88NDD462 pKa = 3.38EE463 pKa = 4.55YY464 pKa = 11.69YY465 pKa = 11.27LNLEE469 pKa = 4.31VGSLSADD476 pKa = 3.64SNILDD481 pKa = 3.42ILLGVGEE488 pKa = 4.2VQIVSSSVSNALCPGEE504 pKa = 5.21SIFLSITDD512 pKa = 3.76FTGYY516 pKa = 10.38SYY518 pKa = 10.38TWFKK522 pKa = 11.31DD523 pKa = 3.75GIPITVSDD531 pKa = 4.33PLNIEE536 pKa = 3.71VSEE539 pKa = 4.08IGSYY543 pKa = 10.85SVTLDD548 pKa = 4.13GFGCQINIAEE558 pKa = 4.16VEE560 pKa = 4.09IVEE563 pKa = 4.61FDD565 pKa = 3.65DD566 pKa = 3.87TVLEE570 pKa = 4.31VSPSTNAVLPVGGSVTFEE588 pKa = 3.64ASGADD593 pKa = 3.23SYY595 pKa = 11.59EE596 pKa = 4.15WYY598 pKa = 10.25DD599 pKa = 3.37EE600 pKa = 4.27AGNLLSTNEE609 pKa = 4.04TLEE612 pKa = 4.07VNTLGTYY619 pKa = 9.85IVIGTVGGCTAQRR632 pKa = 11.84EE633 pKa = 4.63VNVVEE638 pKa = 5.23DD639 pKa = 4.29DD640 pKa = 4.11GKK642 pKa = 11.32LVIPNIITPFNGDD655 pKa = 3.93GINDD659 pKa = 3.16KK660 pKa = 10.73WEE662 pKa = 4.22LPNRR666 pKa = 11.84FAFQTNVQILIFNSKK681 pKa = 10.58GEE683 pKa = 4.22EE684 pKa = 4.02VLNTTDD690 pKa = 3.91YY691 pKa = 10.95QNNWPEE697 pKa = 4.18DD698 pKa = 3.52NNLRR702 pKa = 11.84DD703 pKa = 3.36GMLFYY708 pKa = 10.77FKK710 pKa = 10.67VIRR713 pKa = 11.84EE714 pKa = 4.06NNLIKK719 pKa = 10.77AGTISILQQ727 pKa = 3.5

Molecular weight: 79.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8V9F7|A0A2K8V9F7_9FLAO Histidine kinase OS=Tenacibaculum sp. SZ-18 OX=754423 GN=BTO06_03230 PE=4 SV=1
MM1 pKa = 7.6INGIRR6 pKa = 11.84YY7 pKa = 7.92FFEE10 pKa = 5.13RR11 pKa = 11.84NGFDD15 pKa = 3.1VSSRR19 pKa = 11.84LADD22 pKa = 3.81RR23 pKa = 11.84LGMRR27 pKa = 11.84AVNVRR32 pKa = 11.84LFFIYY37 pKa = 10.25ISFVTVGLSFALYY50 pKa = 10.19LVIAFLLRR58 pKa = 11.84LKK60 pKa = 10.86DD61 pKa = 3.81RR62 pKa = 11.84IYY64 pKa = 10.89TKK66 pKa = 9.75RR67 pKa = 11.84TSVFDD72 pKa = 3.66LL73 pKa = 4.18

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3514

0

3514

1157436

30

8531

329.4

37.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.429 ± 0.043

0.727 ± 0.017

5.441 ± 0.032

6.751 ± 0.049

5.413 ± 0.035

6.08 ± 0.059

1.649 ± 0.022

8.345 ± 0.045

8.605 ± 0.074

9.203 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.954 ± 0.022

6.824 ± 0.038

3.087 ± 0.028

3.23 ± 0.022

3.423 ± 0.029

6.864 ± 0.043

5.744 ± 0.077

6.05 ± 0.039

1.011 ± 0.015

4.171 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski