Penicillium nordicum 
Average proteome isoelectric point is 6.6 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 12938 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A0M8P025|A0A0M8P025_9EURO Uncharacterized protein OS=Penicillium nordicum OX=229535 GN=ACN38_g8358 PE=4 SV=1 
MM1 pKa = 7.13  TFNVFLTGLLAATAAALPHH20 pKa = 5.95  SARR23 pKa = 11.84  SGEE26 pKa = 4.15  SGAVEE31 pKa = 4.25  TRR33 pKa = 11.84  DD34 pKa = 3.57  SSSGGLQIVNNMDD47 pKa = 3.23  TDD49 pKa = 4.02  VYY51 pKa = 11.16  LWTTSSDD58 pKa = 3.31  AGSMKK63 pKa = 10.11  TLSTGGDD70 pKa = 3.79  YY71 pKa = 11.37  SEE73 pKa = 5.77  DD74 pKa = 3.05  WATNSNGGGISIKK87 pKa = 10.18  MSTSEE92 pKa = 4.29  SKK94 pKa = 11.07  DD95 pKa = 3.2  SVLQFEE101 pKa = 4.64  YY102 pKa = 10.25  TQDD105 pKa = 3.43  GDD107 pKa = 4.04  TLFWDD112 pKa = 4.11  MSSIDD117 pKa = 4.43  LDD119 pKa = 3.86  STSEE123 pKa = 4.0  FVKK126 pKa = 10.8  SGFTVVPSDD135 pKa = 4.0  SSCKK139 pKa = 10.14  SVSCAAGDD147 pKa = 4.17  AEE149 pKa = 5.81  CKK151 pKa = 10.0  DD152 pKa = 4.38  AYY154 pKa = 10.57  QLPDD158 pKa = 3.43  DD159 pKa = 4.56  VNTYY163 pKa = 10.49  SCSLSSGFTLTLGG176 pKa = 3.59   
 Molecular weight: 18.44 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.724 
IPC2_protein 3.795 
IPC_protein 3.795 
Toseland    3.567 
ProMoST     3.973 
Dawson      3.795 
Bjellqvist  3.961 
Wikipedia   3.757 
Rodwell     3.617 
Grimsley    3.478 
Solomon     3.783 
Lehninger   3.745 
Nozaki      3.923 
DTASelect   4.19 
Thurlkill   3.63 
EMBOSS      3.77 
Sillero     3.923 
Patrickios  1.901 
IPC_peptide 3.783 
IPC2_peptide  3.884 
IPC2.peptide.svr19  3.819 
 Protein with the highest isoelectric point: 
>tr|A0A0M8P0P1|A0A0M8P0P1_9EURO Non-specific serine/threonine protein kinase OS=Penicillium nordicum OX=229535 GN=ACN38_g10971 PE=4 SV=1 
MM1 pKa = 7.49  PVSPSRR7 pKa = 11.84  TRR9 pKa = 11.84  PSTAGRR15 pKa = 11.84  TMSTTTSVPLPRR27 pKa = 11.84  ARR29 pKa = 11.84  TSVPASSSTTPSALFAPRR47 pKa = 11.84  PGPIVGTVSARR58 pKa = 11.84  LATSLSTLTSRR69 pKa = 5.13   
 Molecular weight: 7.03 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.485 
IPC2_protein 11.067 
IPC_protein 12.691 
Toseland    12.852 
ProMoST     13.349 
Dawson      12.852 
Bjellqvist  12.852 
Wikipedia   13.32 
Rodwell     12.34 
Grimsley    12.881 
Solomon     13.349 
Lehninger   13.247 
Nozaki      12.852 
DTASelect   12.852 
Thurlkill   12.852 
EMBOSS      13.349 
Sillero     12.852 
Patrickios  12.106 
IPC_peptide 13.349 
IPC2_peptide  12.34 
IPC2.peptide.svr19  9.163 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        12938 
 
        
        0
 
        
        12938 
         
        5328948
 
        8
 
        6304
 
        411.9
 
        45.67
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        8.189 ± 0.021
1.283 ± 0.009
 
        5.596 ± 0.018
6.023 ± 0.026
 
        3.872 ± 0.015
6.713 ± 0.02
 
        2.473 ± 0.01
5.168 ± 0.018
       
        4.649 ± 0.02
9.037 ± 0.025
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.272 ± 0.008
3.762 ± 0.012
 
        6.072 ± 0.025
4.048 ± 0.018
 
        5.961 ± 0.02
8.449 ± 0.024
 
        6.021 ± 0.014
6.125 ± 0.016
       
        1.469 ± 0.009
2.814 ± 0.012
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here