Amino acid dipepetide frequency for Pseudomonas phage JG054

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.724AlaAla: 10.724 ± 1.055
1.161AlaCys: 1.161 ± 0.244
4.92AlaAsp: 4.92 ± 0.665
6.247AlaGlu: 6.247 ± 0.771
3.04AlaPhe: 3.04 ± 0.465
7.352AlaGly: 7.352 ± 0.625
1.714AlaHis: 1.714 ± 0.289
5.196AlaIle: 5.196 ± 0.636
5.528AlaLys: 5.528 ± 0.611
10.337AlaLeu: 10.337 ± 0.768
3.206AlaMet: 3.206 ± 0.411
4.256AlaAsn: 4.256 ± 0.492
4.975AlaPro: 4.975 ± 0.951
6.799AlaGln: 6.799 ± 1.095
6.412AlaArg: 6.412 ± 0.619
6.468AlaSer: 6.468 ± 0.59
6.136AlaThr: 6.136 ± 0.651
7.352AlaVal: 7.352 ± 0.719
1.658AlaTrp: 1.658 ± 0.257
3.04AlaTyr: 3.04 ± 0.445
0.0AlaXaa: 0.0 ± 0.0
Cys
0.553CysAla: 0.553 ± 0.191
0.387CysCys: 0.387 ± 0.157
0.94CysAsp: 0.94 ± 0.209
0.884CysGlu: 0.884 ± 0.267
0.498CysPhe: 0.498 ± 0.191
1.106CysGly: 1.106 ± 0.247
0.553CysHis: 0.553 ± 0.168
0.608CysIle: 0.608 ± 0.25
0.553CysLys: 0.553 ± 0.175
0.829CysLeu: 0.829 ± 0.179
0.387CysMet: 0.387 ± 0.14
0.553CysAsn: 0.553 ± 0.2
0.608CysPro: 0.608 ± 0.163
0.442CysGln: 0.442 ± 0.165
0.995CysArg: 0.995 ± 0.27
0.442CysSer: 0.442 ± 0.15
0.498CysThr: 0.498 ± 0.161
0.553CysVal: 0.553 ± 0.171
0.166CysTrp: 0.166 ± 0.105
0.498CysTyr: 0.498 ± 0.194
0.0CysXaa: 0.0 ± 0.0
Asp
5.694AspAla: 5.694 ± 0.468
0.332AspCys: 0.332 ± 0.137
3.261AspAsp: 3.261 ± 0.381
3.317AspGlu: 3.317 ± 0.432
2.598AspPhe: 2.598 ± 0.395
5.141AspGly: 5.141 ± 0.494
1.216AspHis: 1.216 ± 0.24
3.427AspIle: 3.427 ± 0.411
2.764AspLys: 2.764 ± 0.395
5.196AspLeu: 5.196 ± 0.611
1.658AspMet: 1.658 ± 0.249
2.377AspAsn: 2.377 ± 0.35
3.096AspPro: 3.096 ± 0.39
2.211AspGln: 2.211 ± 0.368
3.261AspArg: 3.261 ± 0.431
2.93AspSer: 2.93 ± 0.406
2.488AspThr: 2.488 ± 0.438
3.704AspVal: 3.704 ± 0.438
0.94AspTrp: 0.94 ± 0.216
2.377AspTyr: 2.377 ± 0.361
0.0AspXaa: 0.0 ± 0.0
Glu
7.794GluAla: 7.794 ± 0.827
0.553GluCys: 0.553 ± 0.194
2.875GluAsp: 2.875 ± 0.355
4.201GluGlu: 4.201 ± 0.604
1.99GluPhe: 1.99 ± 0.233
3.925GluGly: 3.925 ± 0.509
1.216GluHis: 1.216 ± 0.307
3.151GluIle: 3.151 ± 0.424
3.483GluLys: 3.483 ± 0.569
6.468GluLeu: 6.468 ± 0.565
2.045GluMet: 2.045 ± 0.333
1.714GluAsn: 1.714 ± 0.357
1.714GluPro: 1.714 ± 0.403
3.427GluGln: 3.427 ± 0.473
3.04GluArg: 3.04 ± 0.492
2.653GluSer: 2.653 ± 0.424
2.764GluThr: 2.764 ± 0.407
4.146GluVal: 4.146 ± 0.47
1.493GluTrp: 1.493 ± 0.31
1.769GluTyr: 1.769 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
3.096PheAla: 3.096 ± 0.417
0.332PheCys: 0.332 ± 0.126
3.096PheAsp: 3.096 ± 0.414
2.045PheGlu: 2.045 ± 0.285
1.382PhePhe: 1.382 ± 0.261
2.377PheGly: 2.377 ± 0.434
1.106PheHis: 1.106 ± 0.209
1.769PheIle: 1.769 ± 0.28
1.658PheLys: 1.658 ± 0.281
2.598PheLeu: 2.598 ± 0.399
1.05PheMet: 1.05 ± 0.258
2.045PheAsn: 2.045 ± 0.33
1.548PhePro: 1.548 ± 0.302
1.05PheGln: 1.05 ± 0.171
2.101PheArg: 2.101 ± 0.307
1.935PheSer: 1.935 ± 0.341
2.211PheThr: 2.211 ± 0.417
2.985PheVal: 2.985 ± 0.463
0.498PheTrp: 0.498 ± 0.19
0.719PheTyr: 0.719 ± 0.209
0.0PheXaa: 0.0 ± 0.0
Gly
7.905GlyAla: 7.905 ± 0.778
0.774GlyCys: 0.774 ± 0.272
3.206GlyAsp: 3.206 ± 0.308
4.256GlyGlu: 4.256 ± 0.508
3.925GlyPhe: 3.925 ± 0.419
6.081GlyGly: 6.081 ± 0.744
1.658GlyHis: 1.658 ± 0.324
4.146GlyIle: 4.146 ± 0.528
4.367GlyLys: 4.367 ± 0.515
5.86GlyLeu: 5.86 ± 0.547
1.99GlyMet: 1.99 ± 0.441
2.156GlyAsn: 2.156 ± 0.255
2.266GlyPro: 2.266 ± 0.358
3.759GlyGln: 3.759 ± 0.501
4.146GlyArg: 4.146 ± 0.39
5.749GlySer: 5.749 ± 0.787
4.92GlyThr: 4.92 ± 0.675
6.302GlyVal: 6.302 ± 0.648
1.161GlyTrp: 1.161 ± 0.243
2.432GlyTyr: 2.432 ± 0.425
0.0GlyXaa: 0.0 ± 0.0
His
2.322HisAla: 2.322 ± 0.334
0.387HisCys: 0.387 ± 0.131
1.161HisAsp: 1.161 ± 0.264
1.05HisGlu: 1.05 ± 0.239
0.884HisPhe: 0.884 ± 0.195
1.382HisGly: 1.382 ± 0.286
0.498HisHis: 0.498 ± 0.177
1.493HisIle: 1.493 ± 0.244
0.884HisLys: 0.884 ± 0.245
1.382HisLeu: 1.382 ± 0.269
0.498HisMet: 0.498 ± 0.186
0.498HisAsn: 0.498 ± 0.167
1.106HisPro: 1.106 ± 0.218
0.221HisGln: 0.221 ± 0.102
1.161HisArg: 1.161 ± 0.257
1.382HisSer: 1.382 ± 0.267
0.995HisThr: 0.995 ± 0.222
1.161HisVal: 1.161 ± 0.283
0.719HisTrp: 0.719 ± 0.245
0.829HisTyr: 0.829 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
5.528IleAla: 5.528 ± 0.6
0.94IleCys: 0.94 ± 0.25
4.312IleAsp: 4.312 ± 0.397
3.206IleGlu: 3.206 ± 0.437
1.161IlePhe: 1.161 ± 0.271
4.312IleGly: 4.312 ± 0.452
0.995IleHis: 0.995 ± 0.198
2.045IleIle: 2.045 ± 0.346
2.432IleLys: 2.432 ± 0.352
3.483IleLeu: 3.483 ± 0.508
1.216IleMet: 1.216 ± 0.234
2.101IleAsn: 2.101 ± 0.327
1.935IlePro: 1.935 ± 0.261
2.322IleGln: 2.322 ± 0.345
3.317IleArg: 3.317 ± 0.465
2.101IleSer: 2.101 ± 0.336
3.372IleThr: 3.372 ± 0.422
4.201IleVal: 4.201 ± 0.508
0.608IleTrp: 0.608 ± 0.182
1.216IleTyr: 1.216 ± 0.237
0.0IleXaa: 0.0 ± 0.0
Lys
6.412LysAla: 6.412 ± 0.905
0.442LysCys: 0.442 ± 0.18
2.93LysAsp: 2.93 ± 0.433
3.317LysGlu: 3.317 ± 0.526
2.045LysPhe: 2.045 ± 0.332
3.261LysGly: 3.261 ± 0.336
0.995LysHis: 0.995 ± 0.196
2.653LysIle: 2.653 ± 0.332
2.543LysLys: 2.543 ± 0.38
4.035LysLeu: 4.035 ± 0.379
1.327LysMet: 1.327 ± 0.289
1.824LysAsn: 1.824 ± 0.293
2.322LysPro: 2.322 ± 0.388
1.493LysGln: 1.493 ± 0.245
2.764LysArg: 2.764 ± 0.282
1.824LysSer: 1.824 ± 0.333
2.598LysThr: 2.598 ± 0.362
3.427LysVal: 3.427 ± 0.389
0.608LysTrp: 0.608 ± 0.181
1.382LysTyr: 1.382 ± 0.287
0.0LysXaa: 0.0 ± 0.0
Leu
7.794LeuAla: 7.794 ± 0.677
0.94LeuCys: 0.94 ± 0.192
5.97LeuAsp: 5.97 ± 0.594
5.694LeuGlu: 5.694 ± 0.495
2.709LeuPhe: 2.709 ± 0.353
6.136LeuGly: 6.136 ± 0.6
2.488LeuHis: 2.488 ± 0.46
3.648LeuIle: 3.648 ± 0.437
4.754LeuLys: 4.754 ± 0.515
6.468LeuLeu: 6.468 ± 0.782
2.101LeuMet: 2.101 ± 0.355
3.096LeuAsn: 3.096 ± 0.398
3.759LeuPro: 3.759 ± 0.457
3.427LeuGln: 3.427 ± 0.523
5.86LeuArg: 5.86 ± 0.59
5.03LeuSer: 5.03 ± 0.598
3.98LeuThr: 3.98 ± 0.487
4.754LeuVal: 4.754 ± 0.521
1.382LeuTrp: 1.382 ± 0.283
1.824LeuTyr: 1.824 ± 0.357
0.0LeuXaa: 0.0 ± 0.0
Met
3.151MetAla: 3.151 ± 0.393
0.332MetCys: 0.332 ± 0.146
1.327MetAsp: 1.327 ± 0.208
1.658MetGlu: 1.658 ± 0.333
0.498MetPhe: 0.498 ± 0.184
1.99MetGly: 1.99 ± 0.37
0.332MetHis: 0.332 ± 0.15
1.05MetIle: 1.05 ± 0.209
1.548MetLys: 1.548 ± 0.27
2.377MetLeu: 2.377 ± 0.437
0.498MetMet: 0.498 ± 0.133
1.216MetAsn: 1.216 ± 0.241
1.603MetPro: 1.603 ± 0.285
1.493MetGln: 1.493 ± 0.296
2.211MetArg: 2.211 ± 0.305
1.658MetSer: 1.658 ± 0.281
2.156MetThr: 2.156 ± 0.293
1.106MetVal: 1.106 ± 0.243
0.442MetTrp: 0.442 ± 0.139
0.829MetTyr: 0.829 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
3.538AsnAla: 3.538 ± 0.462
0.608AsnCys: 0.608 ± 0.183
1.99AsnAsp: 1.99 ± 0.258
2.598AsnGlu: 2.598 ± 0.375
1.216AsnPhe: 1.216 ± 0.232
3.427AsnGly: 3.427 ± 0.496
0.995AsnHis: 0.995 ± 0.229
1.879AsnIle: 1.879 ± 0.298
1.603AsnLys: 1.603 ± 0.24
3.427AsnLeu: 3.427 ± 0.407
0.884AsnMet: 0.884 ± 0.187
2.377AsnAsn: 2.377 ± 0.405
2.322AsnPro: 2.322 ± 0.268
1.327AsnGln: 1.327 ± 0.313
2.322AsnArg: 2.322 ± 0.273
1.769AsnSer: 1.769 ± 0.311
2.598AsnThr: 2.598 ± 0.402
1.99AsnVal: 1.99 ± 0.343
0.884AsnTrp: 0.884 ± 0.171
1.327AsnTyr: 1.327 ± 0.283
0.0AsnXaa: 0.0 ± 0.0
Pro
5.417ProAla: 5.417 ± 0.969
0.719ProCys: 0.719 ± 0.201
3.648ProAsp: 3.648 ± 0.432
2.322ProGlu: 2.322 ± 0.402
1.714ProPhe: 1.714 ± 0.304
2.875ProGly: 2.875 ± 0.388
0.884ProHis: 0.884 ± 0.242
2.432ProIle: 2.432 ± 0.312
1.548ProLys: 1.548 ± 0.26
3.151ProLeu: 3.151 ± 0.375
1.493ProMet: 1.493 ± 0.244
1.879ProAsn: 1.879 ± 0.33
2.322ProPro: 2.322 ± 0.445
1.603ProGln: 1.603 ± 0.476
2.432ProArg: 2.432 ± 0.443
3.206ProSer: 3.206 ± 0.394
3.317ProThr: 3.317 ± 0.355
3.206ProVal: 3.206 ± 0.457
1.106ProTrp: 1.106 ± 0.268
1.493ProTyr: 1.493 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
6.247GlnAla: 6.247 ± 0.663
0.884GlnCys: 0.884 ± 0.311
1.935GlnAsp: 1.935 ± 0.283
2.985GlnGlu: 2.985 ± 0.378
1.382GlnPhe: 1.382 ± 0.285
3.759GlnGly: 3.759 ± 0.63
0.774GlnHis: 0.774 ± 0.248
1.824GlnIle: 1.824 ± 0.388
1.271GlnLys: 1.271 ± 0.203
3.372GlnLeu: 3.372 ± 0.461
1.327GlnMet: 1.327 ± 0.274
2.266GlnAsn: 2.266 ± 0.365
2.543GlnPro: 2.543 ± 0.674
3.483GlnGln: 3.483 ± 1.088
2.875GlnArg: 2.875 ± 0.418
2.543GlnSer: 2.543 ± 0.42
2.377GlnThr: 2.377 ± 0.385
2.377GlnVal: 2.377 ± 0.327
1.05GlnTrp: 1.05 ± 0.224
1.161GlnTyr: 1.161 ± 0.3
0.0GlnXaa: 0.0 ± 0.0
Arg
6.025ArgAla: 6.025 ± 0.699
0.387ArgCys: 0.387 ± 0.149
3.925ArgAsp: 3.925 ± 0.448
4.146ArgGlu: 4.146 ± 0.569
1.769ArgPhe: 1.769 ± 0.33
3.704ArgGly: 3.704 ± 0.483
0.774ArgHis: 0.774 ± 0.191
3.925ArgIle: 3.925 ± 0.475
2.875ArgLys: 2.875 ± 0.459
5.307ArgLeu: 5.307 ± 0.544
1.658ArgMet: 1.658 ± 0.277
1.879ArgAsn: 1.879 ± 0.268
2.653ArgPro: 2.653 ± 0.467
3.372ArgGln: 3.372 ± 0.472
3.814ArgArg: 3.814 ± 0.517
3.98ArgSer: 3.98 ± 0.375
3.483ArgThr: 3.483 ± 0.438
3.427ArgVal: 3.427 ± 0.408
1.271ArgTrp: 1.271 ± 0.255
1.493ArgTyr: 1.493 ± 0.324
0.0ArgXaa: 0.0 ± 0.0
Ser
6.081SerAla: 6.081 ± 0.549
0.774SerCys: 0.774 ± 0.166
2.764SerAsp: 2.764 ± 0.437
2.875SerGlu: 2.875 ± 0.318
2.377SerPhe: 2.377 ± 0.378
6.247SerGly: 6.247 ± 0.646
0.608SerHis: 0.608 ± 0.177
2.985SerIle: 2.985 ± 0.364
2.598SerLys: 2.598 ± 0.384
3.87SerLeu: 3.87 ± 0.552
1.603SerMet: 1.603 ± 0.313
2.211SerAsn: 2.211 ± 0.28
2.598SerPro: 2.598 ± 0.352
1.935SerGln: 1.935 ± 0.305
3.261SerArg: 3.261 ± 0.448
3.317SerSer: 3.317 ± 0.534
3.427SerThr: 3.427 ± 0.43
3.759SerVal: 3.759 ± 0.484
0.774SerTrp: 0.774 ± 0.215
2.156SerTyr: 2.156 ± 0.324
0.0SerXaa: 0.0 ± 0.0
Thr
6.689ThrAla: 6.689 ± 0.645
0.774ThrCys: 0.774 ± 0.151
3.814ThrAsp: 3.814 ± 0.472
2.93ThrGlu: 2.93 ± 0.406
1.879ThrPhe: 1.879 ± 0.335
5.86ThrGly: 5.86 ± 0.602
0.995ThrHis: 0.995 ± 0.248
2.985ThrIle: 2.985 ± 0.44
1.99ThrLys: 1.99 ± 0.339
4.754ThrLeu: 4.754 ± 0.675
1.548ThrMet: 1.548 ± 0.298
2.543ThrAsn: 2.543 ± 0.424
3.538ThrPro: 3.538 ± 0.35
2.211ThrGln: 2.211 ± 0.449
2.432ThrArg: 2.432 ± 0.336
2.764ThrSer: 2.764 ± 0.381
3.814ThrThr: 3.814 ± 0.519
4.588ThrVal: 4.588 ± 0.611
0.553ThrTrp: 0.553 ± 0.143
1.603ThrTyr: 1.603 ± 0.353
0.0ThrXaa: 0.0 ± 0.0
Val
6.799ValAla: 6.799 ± 0.561
0.553ValCys: 0.553 ± 0.161
3.538ValAsp: 3.538 ± 0.491
4.588ValGlu: 4.588 ± 0.531
2.93ValPhe: 2.93 ± 0.439
5.362ValGly: 5.362 ± 0.599
1.161ValHis: 1.161 ± 0.282
3.151ValIle: 3.151 ± 0.469
3.814ValLys: 3.814 ± 0.531
4.754ValLeu: 4.754 ± 0.528
1.714ValMet: 1.714 ± 0.374
2.322ValAsn: 2.322 ± 0.271
3.483ValPro: 3.483 ± 0.391
3.096ValGln: 3.096 ± 0.417
3.925ValArg: 3.925 ± 0.451
3.317ValSer: 3.317 ± 0.496
4.643ValThr: 4.643 ± 0.605
5.252ValVal: 5.252 ± 0.649
1.437ValTrp: 1.437 ± 0.316
2.101ValTyr: 2.101 ± 0.334
0.0ValXaa: 0.0 ± 0.0
Trp
1.935TrpAla: 1.935 ± 0.289
0.553TrpCys: 0.553 ± 0.186
1.05TrpAsp: 1.05 ± 0.231
0.94TrpGlu: 0.94 ± 0.248
0.608TrpPhe: 0.608 ± 0.196
0.608TrpGly: 0.608 ± 0.168
0.276TrpHis: 0.276 ± 0.122
0.774TrpIle: 0.774 ± 0.184
0.774TrpLys: 0.774 ± 0.251
1.548TrpLeu: 1.548 ± 0.273
0.332TrpMet: 0.332 ± 0.138
0.774TrpAsn: 0.774 ± 0.185
0.829TrpPro: 0.829 ± 0.234
0.829TrpGln: 0.829 ± 0.264
1.161TrpArg: 1.161 ± 0.295
0.94TrpSer: 0.94 ± 0.253
0.995TrpThr: 0.995 ± 0.241
1.548TrpVal: 1.548 ± 0.239
0.332TrpTrp: 0.332 ± 0.117
0.829TrpTyr: 0.829 ± 0.194
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.819TyrAla: 2.819 ± 0.403
0.221TyrCys: 0.221 ± 0.109
1.382TyrAsp: 1.382 ± 0.269
1.161TyrGlu: 1.161 ± 0.289
0.995TyrPhe: 0.995 ± 0.232
2.156TyrGly: 2.156 ± 0.414
0.774TyrHis: 0.774 ± 0.209
1.658TyrIle: 1.658 ± 0.315
1.216TyrLys: 1.216 ± 0.263
2.598TyrLeu: 2.598 ± 0.339
0.884TyrMet: 0.884 ± 0.238
0.995TyrAsn: 0.995 ± 0.202
1.548TyrPro: 1.548 ± 0.287
1.935TyrGln: 1.935 ± 0.349
2.377TyrArg: 2.377 ± 0.371
2.211TyrSer: 2.211 ± 0.381
1.493TyrThr: 1.493 ± 0.332
2.101TyrVal: 2.101 ± 0.436
0.553TyrTrp: 0.553 ± 0.275
0.774TyrTyr: 0.774 ± 0.2
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (18091 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski