Amino acid dipepetide frequency for bacterium 0.1xD8-82

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.146AlaAla: 7.146 ± 0.1
1.148AlaCys: 1.148 ± 0.03
4.465AlaAsp: 4.465 ± 0.105
5.171AlaGlu: 5.171 ± 0.077
3.239AlaPhe: 3.239 ± 0.051
6.702AlaGly: 6.702 ± 0.086
1.072AlaHis: 1.072 ± 0.029
4.621AlaIle: 4.621 ± 0.063
4.397AlaLys: 4.397 ± 0.062
6.996AlaLeu: 6.996 ± 0.075
2.279AlaMet: 2.279 ± 0.039
2.343AlaAsn: 2.343 ± 0.053
2.057AlaPro: 2.057 ± 0.045
2.409AlaGln: 2.409 ± 0.042
3.111AlaArg: 3.111 ± 0.048
3.794AlaSer: 3.794 ± 0.061
2.664AlaThr: 2.664 ± 0.05
6.218AlaVal: 6.218 ± 0.073
0.691AlaTrp: 0.691 ± 0.022
2.846AlaTyr: 2.846 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
1.062CysAla: 1.062 ± 0.03
0.307CysCys: 0.307 ± 0.014
0.807CysAsp: 0.807 ± 0.024
0.901CysGlu: 0.901 ± 0.026
0.81CysPhe: 0.81 ± 0.025
1.563CysGly: 1.563 ± 0.038
0.329CysHis: 0.329 ± 0.016
1.211CysIle: 1.211 ± 0.031
0.889CysLys: 0.889 ± 0.027
1.356CysLeu: 1.356 ± 0.034
0.516CysMet: 0.516 ± 0.019
0.652CysAsn: 0.652 ± 0.022
0.614CysPro: 0.614 ± 0.023
0.467CysGln: 0.467 ± 0.018
0.904CysArg: 0.904 ± 0.027
0.959CysSer: 0.959 ± 0.027
0.756CysThr: 0.756 ± 0.024
1.046CysVal: 1.046 ± 0.028
0.153CysTrp: 0.153 ± 0.011
0.666CysTyr: 0.666 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
3.958AspAla: 3.958 ± 0.096
0.796AspCys: 0.796 ± 0.024
2.626AspAsp: 2.626 ± 0.053
4.549AspGlu: 4.549 ± 0.065
2.746AspPhe: 2.746 ± 0.051
4.295AspGly: 4.295 ± 0.071
0.719AspHis: 0.719 ± 0.024
4.479AspIle: 4.479 ± 0.057
3.652AspLys: 3.652 ± 0.06
4.315AspLeu: 4.315 ± 0.069
1.941AspMet: 1.941 ± 0.036
2.267AspAsn: 2.267 ± 0.046
1.516AspPro: 1.516 ± 0.041
1.086AspGln: 1.086 ± 0.028
2.54AspArg: 2.54 ± 0.042
2.895AspSer: 2.895 ± 0.05
2.962AspThr: 2.962 ± 0.049
3.602AspVal: 3.602 ± 0.058
0.633AspTrp: 0.633 ± 0.023
2.892AspTyr: 2.892 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
5.365GluAla: 5.365 ± 0.073
0.853GluCys: 0.853 ± 0.029
4.393GluAsp: 4.393 ± 0.069
7.321GluGlu: 7.321 ± 0.091
2.657GluPhe: 2.657 ± 0.044
4.813GluGly: 4.813 ± 0.061
1.241GluHis: 1.241 ± 0.027
5.827GluIle: 5.827 ± 0.066
7.052GluLys: 7.052 ± 0.094
6.766GluLeu: 6.766 ± 0.075
2.505GluMet: 2.505 ± 0.042
4.474GluAsn: 4.474 ± 0.056
1.869GluPro: 1.869 ± 0.041
2.915GluGln: 2.915 ± 0.052
3.763GluArg: 3.763 ± 0.061
3.665GluSer: 3.665 ± 0.051
3.516GluThr: 3.516 ± 0.059
4.174GluVal: 4.174 ± 0.06
0.781GluTrp: 0.781 ± 0.025
3.257GluTyr: 3.257 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.059PheAla: 3.059 ± 0.048
0.927PheCys: 0.927 ± 0.029
2.447PheAsp: 2.447 ± 0.039
2.777PheGlu: 2.777 ± 0.052
2.237PhePhe: 2.237 ± 0.051
3.102PheGly: 3.102 ± 0.05
0.918PheHis: 0.918 ± 0.029
3.144PheIle: 3.144 ± 0.053
2.109PheLys: 2.109 ± 0.036
4.471PheLeu: 4.471 ± 0.062
1.321PheMet: 1.321 ± 0.031
1.476PheAsn: 1.476 ± 0.034
1.552PhePro: 1.552 ± 0.033
1.43PheGln: 1.43 ± 0.036
1.872PheArg: 1.872 ± 0.034
3.063PheSer: 3.063 ± 0.047
2.391PheThr: 2.391 ± 0.044
2.821PheVal: 2.821 ± 0.048
0.497PheTrp: 0.497 ± 0.022
2.014PheTyr: 2.014 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
4.948GlyAla: 4.948 ± 0.068
1.337GlyCys: 1.337 ± 0.028
3.427GlyAsp: 3.427 ± 0.056
5.137GlyGlu: 5.137 ± 0.065
3.303GlyPhe: 3.303 ± 0.059
4.818GlyGly: 4.818 ± 0.086
1.137GlyHis: 1.137 ± 0.03
6.391GlyIle: 6.391 ± 0.074
5.569GlyLys: 5.569 ± 0.074
6.042GlyLeu: 6.042 ± 0.074
2.608GlyMet: 2.608 ± 0.053
3.367GlyAsn: 3.367 ± 0.054
1.21GlyPro: 1.21 ± 0.035
2.279GlyGln: 2.279 ± 0.042
3.494GlyArg: 3.494 ± 0.054
4.071GlySer: 4.071 ± 0.059
3.922GlyThr: 3.922 ± 0.06
4.524GlyVal: 4.524 ± 0.057
0.746GlyTrp: 0.746 ± 0.025
3.381GlyTyr: 3.381 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.045HisAla: 1.045 ± 0.024
0.268HisCys: 0.268 ± 0.014
0.86HisAsp: 0.86 ± 0.025
1.054HisGlu: 1.054 ± 0.026
0.883HisPhe: 0.883 ± 0.025
1.258HisGly: 1.258 ± 0.032
0.384HisHis: 0.384 ± 0.025
1.461HisIle: 1.461 ± 0.037
1.02HisLys: 1.02 ± 0.03
1.438HisLeu: 1.438 ± 0.037
0.562HisMet: 0.562 ± 0.019
0.73HisAsn: 0.73 ± 0.022
0.805HisPro: 0.805 ± 0.026
0.523HisGln: 0.523 ± 0.02
0.806HisArg: 0.806 ± 0.024
0.968HisSer: 0.968 ± 0.028
0.91HisThr: 0.91 ± 0.026
1.026HisVal: 1.026 ± 0.024
0.163HisTrp: 0.163 ± 0.011
0.834HisTyr: 0.834 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
5.385IleAla: 5.385 ± 0.062
1.485IleCys: 1.485 ± 0.036
3.841IleAsp: 3.841 ± 0.068
4.746IleGlu: 4.746 ± 0.059
3.303IlePhe: 3.303 ± 0.062
4.881IleGly: 4.881 ± 0.062
1.352IleHis: 1.352 ± 0.033
5.087IleIle: 5.087 ± 0.075
4.286IleLys: 4.286 ± 0.063
7.33IleLeu: 7.33 ± 0.089
2.171IleMet: 2.171 ± 0.046
3.019IleAsn: 3.019 ± 0.054
3.213IlePro: 3.213 ± 0.06
2.077IleGln: 2.077 ± 0.042
3.827IleArg: 3.827 ± 0.052
5.153IleSer: 5.153 ± 0.068
4.028IleThr: 4.028 ± 0.057
4.642IleVal: 4.642 ± 0.068
0.764IleTrp: 0.764 ± 0.026
3.053IleTyr: 3.053 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.953LysAla: 4.953 ± 0.06
0.829LysCys: 0.829 ± 0.029
3.819LysAsp: 3.819 ± 0.056
7.168LysGlu: 7.168 ± 0.081
1.891LysPhe: 1.891 ± 0.041
4.437LysGly: 4.437 ± 0.059
0.967LysHis: 0.967 ± 0.026
4.848LysIle: 4.848 ± 0.07
6.018LysLys: 6.018 ± 0.077
5.154LysLeu: 5.154 ± 0.072
2.162LysMet: 2.162 ± 0.042
3.578LysAsn: 3.578 ± 0.052
1.944LysPro: 1.944 ± 0.043
2.32LysGln: 2.32 ± 0.046
3.341LysArg: 3.341 ± 0.053
3.421LysSer: 3.421 ± 0.045
3.393LysThr: 3.393 ± 0.056
4.153LysVal: 4.153 ± 0.059
0.743LysTrp: 0.743 ± 0.022
2.925LysTyr: 2.925 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
6.7LeuAla: 6.7 ± 0.075
1.679LeuCys: 1.679 ± 0.038
4.657LeuAsp: 4.657 ± 0.06
6.258LeuGlu: 6.258 ± 0.076
4.229LeuPhe: 4.229 ± 0.069
5.651LeuGly: 5.651 ± 0.075
1.597LeuHis: 1.597 ± 0.034
6.054LeuIle: 6.054 ± 0.086
6.394LeuLys: 6.394 ± 0.067
9.324LeuLeu: 9.324 ± 0.102
2.725LeuMet: 2.725 ± 0.05
3.934LeuAsn: 3.934 ± 0.049
3.622LeuPro: 3.622 ± 0.054
2.839LeuGln: 2.839 ± 0.048
3.846LeuArg: 3.846 ± 0.058
6.694LeuSer: 6.694 ± 0.078
5.046LeuThr: 5.046 ± 0.071
5.167LeuVal: 5.167 ± 0.078
0.927LeuTrp: 0.927 ± 0.028
3.8LeuTyr: 3.8 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
2.533MetAla: 2.533 ± 0.042
0.403MetCys: 0.403 ± 0.015
1.838MetAsp: 1.838 ± 0.037
2.982MetGlu: 2.982 ± 0.051
1.024MetPhe: 1.024 ± 0.029
2.187MetGly: 2.187 ± 0.042
0.473MetHis: 0.473 ± 0.019
2.163MetIle: 2.163 ± 0.036
2.453MetLys: 2.453 ± 0.042
2.868MetLeu: 2.868 ± 0.046
0.953MetMet: 0.953 ± 0.029
1.499MetAsn: 1.499 ± 0.034
1.194MetPro: 1.194 ± 0.033
1.161MetGln: 1.161 ± 0.031
1.402MetArg: 1.402 ± 0.035
1.733MetSer: 1.733 ± 0.041
1.653MetThr: 1.653 ± 0.037
2.015MetVal: 2.015 ± 0.04
0.282MetTrp: 0.282 ± 0.015
0.942MetTyr: 0.942 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.09AsnAla: 3.09 ± 0.054
0.713AsnCys: 0.713 ± 0.025
2.058AsnAsp: 2.058 ± 0.044
2.834AsnGlu: 2.834 ± 0.043
1.717AsnPhe: 1.717 ± 0.036
3.497AsnGly: 3.497 ± 0.063
0.836AsnHis: 0.836 ± 0.028
3.452AsnIle: 3.452 ± 0.059
2.591AsnLys: 2.591 ± 0.049
3.899AsnLeu: 3.899 ± 0.056
1.476AsnMet: 1.476 ± 0.031
1.837AsnAsn: 1.837 ± 0.05
1.911AsnPro: 1.911 ± 0.039
1.463AsnGln: 1.463 ± 0.033
2.211AsnArg: 2.211 ± 0.046
2.377AsnSer: 2.377 ± 0.045
2.308AsnThr: 2.308 ± 0.043
2.774AsnVal: 2.774 ± 0.05
0.434AsnTrp: 0.434 ± 0.016
1.982AsnTyr: 1.982 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
2.563ProAla: 2.563 ± 0.051
0.476ProCys: 0.476 ± 0.022
2.421ProAsp: 2.421 ± 0.046
3.231ProGlu: 3.231 ± 0.049
1.671ProPhe: 1.671 ± 0.037
2.351ProGly: 2.351 ± 0.045
0.565ProHis: 0.565 ± 0.02
1.855ProIle: 1.855 ± 0.033
1.814ProLys: 1.814 ± 0.041
2.857ProLeu: 2.857 ± 0.055
0.864ProMet: 0.864 ± 0.027
1.114ProAsn: 1.114 ± 0.028
0.733ProPro: 0.733 ± 0.023
0.977ProGln: 0.977 ± 0.03
1.029ProArg: 1.029 ± 0.029
1.687ProSer: 1.687 ± 0.039
1.32ProThr: 1.32 ± 0.037
3.082ProVal: 3.082 ± 0.051
0.344ProTrp: 0.344 ± 0.018
1.57ProTyr: 1.57 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.49GlnAla: 2.49 ± 0.043
0.377GlnCys: 0.377 ± 0.017
1.557GlnAsp: 1.557 ± 0.034
2.904GlnGlu: 2.904 ± 0.053
1.192GlnPhe: 1.192 ± 0.035
2.043GlnGly: 2.043 ± 0.041
0.422GlnHis: 0.422 ± 0.016
2.66GlnIle: 2.66 ± 0.043
2.624GlnLys: 2.624 ± 0.038
2.606GlnLeu: 2.606 ± 0.045
1.183GlnMet: 1.183 ± 0.031
1.676GlnAsn: 1.676 ± 0.034
0.86GlnPro: 0.86 ± 0.027
1.137GlnGln: 1.137 ± 0.035
1.421GlnArg: 1.421 ± 0.036
1.686GlnSer: 1.686 ± 0.042
1.646GlnThr: 1.646 ± 0.034
1.989GlnVal: 1.989 ± 0.036
0.314GlnTrp: 0.314 ± 0.017
1.421GlnTyr: 1.421 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
2.919ArgAla: 2.919 ± 0.048
0.678ArgCys: 0.678 ± 0.022
2.328ArgAsp: 2.328 ± 0.039
4.309ArgGlu: 4.309 ± 0.061
1.943ArgPhe: 1.943 ± 0.042
2.599ArgGly: 2.599 ± 0.046
0.834ArgHis: 0.834 ± 0.023
3.788ArgIle: 3.788 ± 0.053
3.778ArgLys: 3.778 ± 0.061
4.283ArgLeu: 4.283 ± 0.057
1.593ArgMet: 1.593 ± 0.034
2.167ArgAsn: 2.167 ± 0.04
1.386ArgPro: 1.386 ± 0.031
2.014ArgGln: 2.014 ± 0.043
2.476ArgArg: 2.476 ± 0.048
2.166ArgSer: 2.166 ± 0.041
2.19ArgThr: 2.19 ± 0.043
2.611ArgVal: 2.611 ± 0.044
0.453ArgTrp: 0.453 ± 0.018
2.08ArgTyr: 2.08 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
4.267SerAla: 4.267 ± 0.076
0.887SerCys: 0.887 ± 0.023
3.237SerAsp: 3.237 ± 0.057
3.806SerGlu: 3.806 ± 0.052
2.942SerPhe: 2.942 ± 0.049
5.117SerGly: 5.117 ± 0.067
1.083SerHis: 1.083 ± 0.029
4.106SerIle: 4.106 ± 0.058
3.196SerLys: 3.196 ± 0.043
5.422SerLeu: 5.422 ± 0.059
1.863SerMet: 1.863 ± 0.038
2.185SerAsn: 2.185 ± 0.042
1.869SerPro: 1.869 ± 0.045
1.748SerGln: 1.748 ± 0.04
2.91SerArg: 2.91 ± 0.043
3.375SerSer: 3.375 ± 0.061
2.552SerThr: 2.552 ± 0.054
4.219SerVal: 4.219 ± 0.055
0.547SerTrp: 0.547 ± 0.022
2.518SerTyr: 2.518 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
4.158ThrAla: 4.158 ± 0.063
0.661ThrCys: 0.661 ± 0.023
3.061ThrAsp: 3.061 ± 0.045
3.533ThrGlu: 3.533 ± 0.055
2.138ThrPhe: 2.138 ± 0.043
4.598ThrGly: 4.598 ± 0.066
0.825ThrHis: 0.825 ± 0.029
3.65ThrIle: 3.65 ± 0.06
2.666ThrLys: 2.666 ± 0.05
4.488ThrLeu: 4.488 ± 0.065
1.378ThrMet: 1.378 ± 0.03
1.87ThrAsn: 1.87 ± 0.038
1.994ThrPro: 1.994 ± 0.045
1.444ThrGln: 1.444 ± 0.033
1.988ThrArg: 1.988 ± 0.038
2.641ThrSer: 2.641 ± 0.053
2.255ThrThr: 2.255 ± 0.054
4.023ThrVal: 4.023 ± 0.066
0.506ThrTrp: 0.506 ± 0.022
1.984ThrTyr: 1.984 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
4.245ValAla: 4.245 ± 0.059
1.245ValCys: 1.245 ± 0.032
3.489ValAsp: 3.489 ± 0.05
4.43ValGlu: 4.43 ± 0.063
3.137ValPhe: 3.137 ± 0.061
3.789ValGly: 3.789 ± 0.063
1.063ValHis: 1.063 ± 0.032
5.099ValIle: 5.099 ± 0.061
4.388ValLys: 4.388 ± 0.047
6.423ValLeu: 6.423 ± 0.082
2.052ValMet: 2.052 ± 0.042
2.842ValAsn: 2.842 ± 0.049
2.361ValPro: 2.361 ± 0.041
1.873ValGln: 1.873 ± 0.038
3.047ValArg: 3.047 ± 0.05
4.559ValSer: 4.559 ± 0.063
3.792ValThr: 3.792 ± 0.065
4.369ValVal: 4.369 ± 0.063
0.739ValTrp: 0.739 ± 0.024
2.781ValTyr: 2.781 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
0.613TrpAla: 0.613 ± 0.023
0.177TrpCys: 0.177 ± 0.011
0.602TrpAsp: 0.602 ± 0.02
0.827TrpGlu: 0.827 ± 0.029
0.444TrpPhe: 0.444 ± 0.02
0.782TrpGly: 0.782 ± 0.03
0.226TrpHis: 0.226 ± 0.013
0.735TrpIle: 0.735 ± 0.024
0.816TrpLys: 0.816 ± 0.026
0.932TrpLeu: 0.932 ± 0.029
0.323TrpMet: 0.323 ± 0.016
0.586TrpAsn: 0.586 ± 0.02
0.246TrpPro: 0.246 ± 0.016
0.459TrpGln: 0.459 ± 0.018
0.401TrpArg: 0.401 ± 0.018
0.524TrpSer: 0.524 ± 0.02
0.428TrpThr: 0.428 ± 0.019
0.566TrpVal: 0.566 ± 0.021
0.161TrpTrp: 0.161 ± 0.012
0.464TrpTyr: 0.464 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.868TyrAla: 2.868 ± 0.045
0.723TyrCys: 0.723 ± 0.024
2.536TyrAsp: 2.536 ± 0.039
3.268TyrGlu: 3.268 ± 0.058
2.123TyrPhe: 2.123 ± 0.04
3.228TyrGly: 3.228 ± 0.057
0.957TyrHis: 0.957 ± 0.024
3.011TyrIle: 3.011 ± 0.054
2.356TyrLys: 2.356 ± 0.047
4.168TyrLeu: 4.168 ± 0.058
1.283TyrMet: 1.283 ± 0.03
1.845TyrAsn: 1.845 ± 0.038
1.565TyrPro: 1.565 ± 0.037
1.587TyrGln: 1.587 ± 0.035
2.207TyrArg: 2.207 ± 0.039
2.419TyrSer: 2.419 ± 0.041
2.129TyrThr: 2.129 ± 0.043
2.702TyrVal: 2.702 ± 0.049
0.435TyrTrp: 0.435 ± 0.02
2.097TyrTyr: 2.097 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4335 proteins (1345899 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski