Nocardioides sp. Root614

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4780 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q8DUZ2|A0A0Q8DUZ2_9ACTN Multidrug MFS transporter OS=Nocardioides sp. Root614 OX=1736571 GN=ASD81_15580 PE=4 SV=1
MM1 pKa = 7.33TPASLHH7 pKa = 5.7LFSRR11 pKa = 11.84DD12 pKa = 3.13RR13 pKa = 11.84LATRR17 pKa = 11.84VTAVGLFACGSWALAFSATAAAPASADD44 pKa = 3.64AGDD47 pKa = 4.05LTCHH51 pKa = 6.15GKK53 pKa = 10.22AVTILVDD60 pKa = 3.93ASHH63 pKa = 6.75NGSPVYY69 pKa = 10.43GSSGDD74 pKa = 3.8DD75 pKa = 3.21VIMAFGGGHH84 pKa = 6.92HH85 pKa = 7.37IDD87 pKa = 3.59GQAGNDD93 pKa = 4.17TICASSDD100 pKa = 3.3VGSVGDD106 pKa = 4.05VILGGVGNDD115 pKa = 3.66YY116 pKa = 9.95LTGGAGNDD124 pKa = 3.8TLDD127 pKa = 4.09GGSDD131 pKa = 3.13QDD133 pKa = 4.76FIGGGPGDD141 pKa = 4.5DD142 pKa = 3.57NLFGGDD148 pKa = 3.8HH149 pKa = 6.15VDD151 pKa = 3.46EE152 pKa = 4.76LRR154 pKa = 11.84GGSGDD159 pKa = 4.05DD160 pKa = 3.39RR161 pKa = 11.84LTGGDD166 pKa = 4.33ARR168 pKa = 11.84DD169 pKa = 3.68MLYY172 pKa = 10.9GDD174 pKa = 4.41SGDD177 pKa = 5.06DD178 pKa = 3.72VIHH181 pKa = 6.81GNDD184 pKa = 4.1GGDD187 pKa = 3.21GVYY190 pKa = 10.82GQDD193 pKa = 5.06GNDD196 pKa = 3.42HH197 pKa = 7.07LYY199 pKa = 11.38GEE201 pKa = 4.92IGQDD205 pKa = 3.42VLVGGLGDD213 pKa = 4.5DD214 pKa = 4.16LLHH217 pKa = 6.87GGLYY221 pKa = 9.9PDD223 pKa = 3.86SLYY226 pKa = 11.03GQEE229 pKa = 4.51GDD231 pKa = 3.77DD232 pKa = 4.75VIYY235 pKa = 10.5TGDD238 pKa = 3.72GGPAGFDD245 pKa = 3.2KK246 pKa = 11.36AFGDD250 pKa = 3.74SGNDD254 pKa = 3.44VLHH257 pKa = 6.73GSSDD261 pKa = 3.71GDD263 pKa = 3.57RR264 pKa = 11.84LNGGTGNDD272 pKa = 3.54LLYY275 pKa = 10.84GYY277 pKa = 10.51GSQDD281 pKa = 2.95KK282 pKa = 10.93LYY284 pKa = 10.63GDD286 pKa = 4.19SGNDD290 pKa = 3.14RR291 pKa = 11.84LEE293 pKa = 4.4GGGNRR298 pKa = 11.84DD299 pKa = 3.4EE300 pKa = 4.93LRR302 pKa = 11.84GHH304 pKa = 6.72EE305 pKa = 4.53GNDD308 pKa = 3.01RR309 pKa = 11.84LYY311 pKa = 11.2GGDD314 pKa = 4.03GADD317 pKa = 4.13DD318 pKa = 3.56LHH320 pKa = 8.69GGVNDD325 pKa = 4.75DD326 pKa = 3.99YY327 pKa = 11.9LNGGGDD333 pKa = 5.74LMDD336 pKa = 4.82QGNGDD341 pKa = 4.32PGVDD345 pKa = 3.11TCVGIRR351 pKa = 11.84DD352 pKa = 3.87AMQTTCEE359 pKa = 3.9RR360 pKa = 3.79

Molecular weight: 36.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q8D7T6|A0A0Q8D7T6_9ACTN Uncharacterized protein OS=Nocardioides sp. Root614 OX=1736571 GN=ASD81_24350 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84PVSGIGSRR10 pKa = 11.84RR11 pKa = 11.84SVEE14 pKa = 3.4QWDD17 pKa = 3.74RR18 pKa = 11.84FFEE21 pKa = 4.36LVCAGASVSAAAAEE35 pKa = 4.17IGVARR40 pKa = 11.84QSAWEE45 pKa = 4.0RR46 pKa = 11.84WRR48 pKa = 11.84NSAPMEE54 pKa = 4.27LKK56 pKa = 10.57LLQGRR61 pKa = 11.84GGGLGQPAEE70 pKa = 4.49CEE72 pKa = 3.99QVAGRR77 pKa = 11.84VRR79 pKa = 11.84RR80 pKa = 11.84ALTSEE85 pKa = 3.7DD86 pKa = 3.33RR87 pKa = 11.84AVIAAGVRR95 pKa = 11.84KK96 pKa = 9.26KK97 pKa = 7.42WTYY100 pKa = 11.46AEE102 pKa = 3.87IGEE105 pKa = 4.61AIGRR109 pKa = 11.84SPSVICRR116 pKa = 11.84EE117 pKa = 3.94VARR120 pKa = 11.84NKK122 pKa = 10.72GPDD125 pKa = 3.41GSYY128 pKa = 10.5HH129 pKa = 5.55GTLAHH134 pKa = 6.62AAARR138 pKa = 11.84QRR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84RR143 pKa = 11.84PKK145 pKa = 9.45EE146 pKa = 3.46FRR148 pKa = 11.84LVAEE152 pKa = 4.83PGLCRR157 pKa = 11.84RR158 pKa = 11.84IEE160 pKa = 3.74TWMDD164 pKa = 3.31EE165 pKa = 3.91GWSPKK170 pKa = 10.34LIARR174 pKa = 11.84VLRR177 pKa = 11.84EE178 pKa = 3.81EE179 pKa = 4.28SPRR182 pKa = 11.84MIMGRR187 pKa = 11.84VSHH190 pKa = 5.25EE191 pKa = 4.14TIYY194 pKa = 10.62QALYY198 pKa = 8.06VQTRR202 pKa = 11.84GSLRR206 pKa = 11.84KK207 pKa = 9.51DD208 pKa = 3.92LYY210 pKa = 10.57RR211 pKa = 11.84QLSTTRR217 pKa = 11.84PRR219 pKa = 11.84RR220 pKa = 11.84QPRR223 pKa = 11.84VGAKK227 pKa = 8.19RR228 pKa = 11.84TNSPYY233 pKa = 10.62KK234 pKa = 9.66EE235 pKa = 3.99AFKK238 pKa = 10.55ISQRR242 pKa = 11.84PAEE245 pKa = 4.27VADD248 pKa = 4.12RR249 pKa = 11.84AVPGHH254 pKa = 6.24WEE256 pKa = 3.87GDD258 pKa = 3.61LVMGTAGGVAVGTLVEE274 pKa = 4.19RR275 pKa = 11.84STRR278 pKa = 11.84FTILLHH284 pKa = 6.34LPGRR288 pKa = 11.84HH289 pKa = 5.98DD290 pKa = 4.16AEE292 pKa = 4.37SVAAAMIRR300 pKa = 11.84EE301 pKa = 4.11MSEE304 pKa = 3.95LPEE307 pKa = 4.33HH308 pKa = 6.71LRR310 pKa = 11.84QSLTWDD316 pKa = 3.27RR317 pKa = 11.84GTEE320 pKa = 3.67LARR323 pKa = 11.84YY324 pKa = 9.58DD325 pKa = 5.12KK326 pKa = 10.86IQTDD330 pKa = 3.85LAMPVYY336 pKa = 10.35FCDD339 pKa = 4.35PHH341 pKa = 8.5APWQRR346 pKa = 11.84GTNEE350 pKa = 3.5NTNRR354 pKa = 11.84LLRR357 pKa = 11.84HH358 pKa = 6.0WLTKK362 pKa = 9.89GTDD365 pKa = 3.39LSRR368 pKa = 11.84FSAADD373 pKa = 3.35LRR375 pKa = 11.84RR376 pKa = 11.84IAASLNARR384 pKa = 11.84PRR386 pKa = 11.84PTLNMQTPAQALDD399 pKa = 3.53QLLTNPAAAA408 pKa = 4.6

Molecular weight: 45.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4780

0

4780

1577883

37

2876

330.1

35.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.864 ± 0.048

0.747 ± 0.01

6.605 ± 0.034

5.647 ± 0.034

2.923 ± 0.02

9.133 ± 0.032

2.121 ± 0.018

3.947 ± 0.022

2.272 ± 0.027

10.059 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.81 ± 0.013

2.066 ± 0.022

5.491 ± 0.027

2.816 ± 0.018

7.106 ± 0.042

5.449 ± 0.024

6.262 ± 0.041

9.191 ± 0.033

1.49 ± 0.014

2.0 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski