Amino acid dipepetide frequency for Microbacterium phage vB_MoxS-ISF9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.774AlaAla: 12.774 ± 1.157
0.541AlaCys: 0.541 ± 0.19
5.9AlaAsp: 5.9 ± 0.554
7.307AlaGlu: 7.307 ± 0.702
2.869AlaPhe: 2.869 ± 0.353
9.039AlaGly: 9.039 ± 1.035
2.219AlaHis: 2.219 ± 0.351
5.575AlaIle: 5.575 ± 0.618
5.737AlaLys: 5.737 ± 0.77
8.606AlaLeu: 8.606 ± 0.75
4.33AlaMet: 4.33 ± 0.412
3.464AlaAsn: 3.464 ± 0.46
3.518AlaPro: 3.518 ± 0.422
3.951AlaGln: 3.951 ± 0.481
7.091AlaArg: 7.091 ± 0.6
6.495AlaSer: 6.495 ± 0.721
5.737AlaThr: 5.737 ± 0.69
7.145AlaVal: 7.145 ± 0.602
2.219AlaTrp: 2.219 ± 0.299
2.76AlaTyr: 2.76 ± 0.398
0.0AlaXaa: 0.0 ± 0.0
Cys
0.595CysAla: 0.595 ± 0.198
0.054CysCys: 0.054 ± 0.059
0.758CysAsp: 0.758 ± 0.275
0.325CysGlu: 0.325 ± 0.151
0.325CysPhe: 0.325 ± 0.136
0.866CysGly: 0.866 ± 0.25
0.217CysHis: 0.217 ± 0.104
0.433CysIle: 0.433 ± 0.139
0.433CysLys: 0.433 ± 0.152
0.217CysLeu: 0.217 ± 0.113
0.054CysMet: 0.054 ± 0.05
0.162CysAsn: 0.162 ± 0.073
0.325CysPro: 0.325 ± 0.133
0.108CysGln: 0.108 ± 0.075
0.433CysArg: 0.433 ± 0.16
0.217CysSer: 0.217 ± 0.104
0.054CysThr: 0.054 ± 0.058
0.379CysVal: 0.379 ± 0.141
0.162CysTrp: 0.162 ± 0.085
0.108CysTyr: 0.108 ± 0.074
0.0CysXaa: 0.0 ± 0.0
Asp
7.524AspAla: 7.524 ± 0.514
0.379AspCys: 0.379 ± 0.131
3.735AspAsp: 3.735 ± 0.452
4.438AspGlu: 4.438 ± 0.53
2.327AspPhe: 2.327 ± 0.351
6.82AspGly: 6.82 ± 0.725
0.974AspHis: 0.974 ± 0.249
2.706AspIle: 2.706 ± 0.426
3.302AspLys: 3.302 ± 0.515
5.088AspLeu: 5.088 ± 0.471
1.624AspMet: 1.624 ± 0.291
1.57AspAsn: 1.57 ± 0.282
3.843AspPro: 3.843 ± 0.572
1.461AspGln: 1.461 ± 0.274
3.41AspArg: 3.41 ± 0.453
4.384AspSer: 4.384 ± 0.5
3.248AspThr: 3.248 ± 0.382
3.464AspVal: 3.464 ± 0.38
1.353AspTrp: 1.353 ± 0.274
2.706AspTyr: 2.706 ± 0.332
0.0AspXaa: 0.0 ± 0.0
Glu
7.524GluAla: 7.524 ± 0.608
0.595GluCys: 0.595 ± 0.236
4.655GluAsp: 4.655 ± 0.473
5.304GluGlu: 5.304 ± 0.673
2.869GluPhe: 2.869 ± 0.368
5.304GluGly: 5.304 ± 0.485
1.137GluHis: 1.137 ± 0.258
4.547GluIle: 4.547 ± 0.479
3.572GluLys: 3.572 ± 0.535
4.871GluLeu: 4.871 ± 0.49
1.949GluMet: 1.949 ± 0.38
2.436GluAsn: 2.436 ± 0.343
2.003GluPro: 2.003 ± 0.363
2.706GluGln: 2.706 ± 0.364
5.467GluArg: 5.467 ± 0.645
4.709GluSer: 4.709 ± 0.535
2.977GluThr: 2.977 ± 0.496
6.008GluVal: 6.008 ± 0.763
2.003GluTrp: 2.003 ± 0.327
2.003GluTyr: 2.003 ± 0.31
0.0GluXaa: 0.0 ± 0.0
Phe
3.518PheAla: 3.518 ± 0.435
0.108PheCys: 0.108 ± 0.071
2.219PheAsp: 2.219 ± 0.381
2.327PheGlu: 2.327 ± 0.366
0.704PhePhe: 0.704 ± 0.214
3.302PheGly: 3.302 ± 0.369
0.379PheHis: 0.379 ± 0.141
1.461PheIle: 1.461 ± 0.312
1.407PheLys: 1.407 ± 0.272
2.057PheLeu: 2.057 ± 0.333
0.812PheMet: 0.812 ± 0.195
0.92PheAsn: 0.92 ± 0.216
0.974PhePro: 0.974 ± 0.224
1.028PheGln: 1.028 ± 0.228
1.732PheArg: 1.732 ± 0.283
1.786PheSer: 1.786 ± 0.336
2.327PheThr: 2.327 ± 0.467
2.165PheVal: 2.165 ± 0.357
0.812PheTrp: 0.812 ± 0.181
1.028PheTyr: 1.028 ± 0.285
0.0PheXaa: 0.0 ± 0.0
Gly
7.47GlyAla: 7.47 ± 0.894
0.433GlyCys: 0.433 ± 0.165
4.709GlyAsp: 4.709 ± 0.411
6.387GlyGlu: 6.387 ± 0.593
3.681GlyPhe: 3.681 ± 0.437
6.712GlyGly: 6.712 ± 0.666
1.786GlyHis: 1.786 ± 0.384
4.276GlyIle: 4.276 ± 0.506
3.627GlyLys: 3.627 ± 0.411
6.062GlyLeu: 6.062 ± 0.971
2.003GlyMet: 2.003 ± 0.32
3.139GlyAsn: 3.139 ± 0.48
2.76GlyPro: 2.76 ± 0.446
3.031GlyGln: 3.031 ± 0.397
5.629GlyArg: 5.629 ± 0.677
5.737GlySer: 5.737 ± 0.633
5.629GlyThr: 5.629 ± 0.534
7.361GlyVal: 7.361 ± 0.638
2.057GlyTrp: 2.057 ± 0.381
2.706GlyTyr: 2.706 ± 0.497
0.0GlyXaa: 0.0 ± 0.0
His
2.219HisAla: 2.219 ± 0.355
0.108HisCys: 0.108 ± 0.085
1.191HisAsp: 1.191 ± 0.238
1.299HisGlu: 1.299 ± 0.259
0.866HisPhe: 0.866 ± 0.251
1.245HisGly: 1.245 ± 0.295
0.433HisHis: 0.433 ± 0.147
0.92HisIle: 0.92 ± 0.226
0.812HisLys: 0.812 ± 0.175
1.894HisLeu: 1.894 ± 0.331
0.487HisMet: 0.487 ± 0.163
0.487HisAsn: 0.487 ± 0.143
1.299HisPro: 1.299 ± 0.277
0.704HisGln: 0.704 ± 0.202
0.92HisArg: 0.92 ± 0.186
0.812HisSer: 0.812 ± 0.214
1.137HisThr: 1.137 ± 0.258
1.84HisVal: 1.84 ± 0.265
0.65HisTrp: 0.65 ± 0.192
0.595HisTyr: 0.595 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
5.088IleAla: 5.088 ± 0.557
0.108IleCys: 0.108 ± 0.071
3.085IleAsp: 3.085 ± 0.394
5.575IleGlu: 5.575 ± 0.651
1.732IlePhe: 1.732 ± 0.275
4.005IleGly: 4.005 ± 0.524
1.028IleHis: 1.028 ± 0.295
1.624IleIle: 1.624 ± 0.325
2.057IleLys: 2.057 ± 0.283
3.951IleLeu: 3.951 ± 0.504
0.812IleMet: 0.812 ± 0.173
2.165IleAsn: 2.165 ± 0.333
1.894IlePro: 1.894 ± 0.284
2.111IleGln: 2.111 ± 0.347
2.869IleArg: 2.869 ± 0.349
2.327IleSer: 2.327 ± 0.367
3.356IleThr: 3.356 ± 0.412
3.789IleVal: 3.789 ± 0.506
0.595IleTrp: 0.595 ± 0.198
1.299IleTyr: 1.299 ± 0.275
0.0IleXaa: 0.0 ± 0.0
Lys
5.792LysAla: 5.792 ± 0.649
0.162LysCys: 0.162 ± 0.082
2.273LysAsp: 2.273 ± 0.436
3.031LysGlu: 3.031 ± 0.337
1.299LysPhe: 1.299 ± 0.301
3.572LysGly: 3.572 ± 0.461
0.974LysHis: 0.974 ± 0.261
2.382LysIle: 2.382 ± 0.334
2.111LysLys: 2.111 ± 0.465
3.951LysLeu: 3.951 ± 0.487
1.786LysMet: 1.786 ± 0.303
1.191LysAsn: 1.191 ± 0.267
1.949LysPro: 1.949 ± 0.332
1.516LysGln: 1.516 ± 0.287
3.518LysArg: 3.518 ± 0.518
2.003LysSer: 2.003 ± 0.366
2.923LysThr: 2.923 ± 0.473
3.735LysVal: 3.735 ± 0.429
0.812LysTrp: 0.812 ± 0.189
1.461LysTyr: 1.461 ± 0.269
0.0LysXaa: 0.0 ± 0.0
Leu
8.444LeuAla: 8.444 ± 0.594
0.92LeuCys: 0.92 ± 0.271
5.575LeuAsp: 5.575 ± 0.433
5.196LeuGlu: 5.196 ± 0.625
1.84LeuPhe: 1.84 ± 0.343
4.98LeuGly: 4.98 ± 0.54
1.137LeuHis: 1.137 ± 0.284
2.706LeuIle: 2.706 ± 0.399
3.41LeuLys: 3.41 ± 0.406
4.222LeuLeu: 4.222 ± 0.459
2.003LeuMet: 2.003 ± 0.381
3.194LeuAsn: 3.194 ± 0.721
2.977LeuPro: 2.977 ± 0.446
1.678LeuGln: 1.678 ± 0.308
4.709LeuArg: 4.709 ± 0.4
4.06LeuSer: 4.06 ± 0.585
4.871LeuThr: 4.871 ± 0.557
4.763LeuVal: 4.763 ± 0.41
1.353LeuTrp: 1.353 ± 0.251
2.382LeuTyr: 2.382 ± 0.331
0.0LeuXaa: 0.0 ± 0.0
Met
2.76MetAla: 2.76 ± 0.46
0.0MetCys: 0.0 ± 0.0
1.461MetAsp: 1.461 ± 0.308
1.191MetGlu: 1.191 ± 0.235
0.974MetPhe: 0.974 ± 0.233
2.219MetGly: 2.219 ± 0.338
0.433MetHis: 0.433 ± 0.15
1.894MetIle: 1.894 ± 0.473
1.732MetLys: 1.732 ± 0.318
1.245MetLeu: 1.245 ± 0.308
0.217MetMet: 0.217 ± 0.122
0.65MetAsn: 0.65 ± 0.177
1.191MetPro: 1.191 ± 0.26
0.866MetGln: 0.866 ± 0.215
1.407MetArg: 1.407 ± 0.311
2.815MetSer: 2.815 ± 0.385
3.085MetThr: 3.085 ± 0.378
1.191MetVal: 1.191 ± 0.301
0.433MetTrp: 0.433 ± 0.183
0.812MetTyr: 0.812 ± 0.238
0.0MetXaa: 0.0 ± 0.0
Asn
2.977AsnAla: 2.977 ± 0.423
0.162AsnCys: 0.162 ± 0.096
2.057AsnAsp: 2.057 ± 0.335
1.624AsnGlu: 1.624 ± 0.293
1.245AsnPhe: 1.245 ± 0.257
3.356AsnGly: 3.356 ± 0.401
0.974AsnHis: 0.974 ± 0.197
1.299AsnIle: 1.299 ± 0.252
1.191AsnLys: 1.191 ± 0.256
2.49AsnLeu: 2.49 ± 0.387
0.758AsnMet: 0.758 ± 0.236
1.353AsnAsn: 1.353 ± 0.313
2.327AsnPro: 2.327 ± 0.329
1.083AsnGln: 1.083 ± 0.185
2.219AsnArg: 2.219 ± 0.35
2.382AsnSer: 2.382 ± 0.481
1.353AsnThr: 1.353 ± 0.248
2.544AsnVal: 2.544 ± 0.377
0.541AsnTrp: 0.541 ± 0.163
1.461AsnTyr: 1.461 ± 0.264
0.0AsnXaa: 0.0 ± 0.0
Pro
3.627ProAla: 3.627 ± 0.47
0.325ProCys: 0.325 ± 0.126
3.518ProAsp: 3.518 ± 0.486
4.114ProGlu: 4.114 ± 0.534
1.353ProPhe: 1.353 ± 0.258
3.41ProGly: 3.41 ± 0.528
1.299ProHis: 1.299 ± 0.246
1.894ProIle: 1.894 ± 0.322
2.111ProLys: 2.111 ± 0.454
2.652ProLeu: 2.652 ± 0.385
1.299ProMet: 1.299 ± 0.316
1.191ProAsn: 1.191 ± 0.21
2.003ProPro: 2.003 ± 0.424
1.353ProGln: 1.353 ± 0.224
2.327ProArg: 2.327 ± 0.312
2.923ProSer: 2.923 ± 0.434
3.41ProThr: 3.41 ± 0.472
3.627ProVal: 3.627 ± 0.424
0.866ProTrp: 0.866 ± 0.264
1.083ProTyr: 1.083 ± 0.235
0.0ProXaa: 0.0 ± 0.0
Gln
3.951GlnAla: 3.951 ± 0.432
0.054GlnCys: 0.054 ± 0.057
1.84GlnAsp: 1.84 ± 0.255
2.598GlnGlu: 2.598 ± 0.345
0.595GlnPhe: 0.595 ± 0.181
2.165GlnGly: 2.165 ± 0.354
0.541GlnHis: 0.541 ± 0.165
2.003GlnIle: 2.003 ± 0.351
1.083GlnLys: 1.083 ± 0.317
1.786GlnLeu: 1.786 ± 0.376
1.137GlnMet: 1.137 ± 0.271
1.028GlnAsn: 1.028 ± 0.242
0.92GlnPro: 0.92 ± 0.25
1.028GlnGln: 1.028 ± 0.289
1.84GlnArg: 1.84 ± 0.326
2.003GlnSer: 2.003 ± 0.379
1.949GlnThr: 1.949 ± 0.38
3.41GlnVal: 3.41 ± 0.394
0.704GlnTrp: 0.704 ± 0.165
0.812GlnTyr: 0.812 ± 0.21
0.0GlnXaa: 0.0 ± 0.0
Arg
6.712ArgAla: 6.712 ± 0.707
0.433ArgCys: 0.433 ± 0.135
4.005ArgAsp: 4.005 ± 0.412
4.547ArgGlu: 4.547 ± 0.591
1.786ArgPhe: 1.786 ± 0.283
4.817ArgGly: 4.817 ± 0.464
1.624ArgHis: 1.624 ± 0.245
3.572ArgIle: 3.572 ± 0.377
3.194ArgLys: 3.194 ± 0.494
5.034ArgLeu: 5.034 ± 0.471
1.732ArgMet: 1.732 ± 0.329
2.273ArgAsn: 2.273 ± 0.371
2.923ArgPro: 2.923 ± 0.408
1.624ArgGln: 1.624 ± 0.265
5.034ArgArg: 5.034 ± 0.73
3.139ArgSer: 3.139 ± 0.412
3.139ArgThr: 3.139 ± 0.468
5.196ArgVal: 5.196 ± 0.644
1.57ArgTrp: 1.57 ± 0.244
2.436ArgTyr: 2.436 ± 0.37
0.0ArgXaa: 0.0 ± 0.0
Ser
5.846SerAla: 5.846 ± 0.711
0.379SerCys: 0.379 ± 0.153
3.572SerAsp: 3.572 ± 0.379
4.493SerGlu: 4.493 ± 0.55
2.057SerPhe: 2.057 ± 0.315
6.333SerGly: 6.333 ± 0.639
1.137SerHis: 1.137 ± 0.229
2.76SerIle: 2.76 ± 0.375
2.598SerLys: 2.598 ± 0.347
3.843SerLeu: 3.843 ± 0.493
1.461SerMet: 1.461 ± 0.297
1.894SerAsn: 1.894 ± 0.35
2.76SerPro: 2.76 ± 0.481
1.786SerGln: 1.786 ± 0.276
3.41SerArg: 3.41 ± 0.389
3.302SerSer: 3.302 ± 0.528
4.005SerThr: 4.005 ± 0.419
5.142SerVal: 5.142 ± 0.706
1.083SerTrp: 1.083 ± 0.231
1.949SerTyr: 1.949 ± 0.347
0.0SerXaa: 0.0 ± 0.0
Thr
5.575ThrAla: 5.575 ± 0.625
0.541ThrCys: 0.541 ± 0.195
3.302ThrAsp: 3.302 ± 0.498
3.951ThrGlu: 3.951 ± 0.408
1.624ThrPhe: 1.624 ± 0.326
6.225ThrGly: 6.225 ± 0.67
1.407ThrHis: 1.407 ± 0.272
3.356ThrIle: 3.356 ± 0.473
2.382ThrLys: 2.382 ± 0.4
4.276ThrLeu: 4.276 ± 0.541
1.245ThrMet: 1.245 ± 0.283
1.84ThrAsn: 1.84 ± 0.341
4.493ThrPro: 4.493 ± 0.529
1.732ThrGln: 1.732 ± 0.303
3.735ThrArg: 3.735 ± 0.462
3.139ThrSer: 3.139 ± 0.438
3.464ThrThr: 3.464 ± 0.498
4.601ThrVal: 4.601 ± 0.538
1.191ThrTrp: 1.191 ± 0.217
2.111ThrTyr: 2.111 ± 0.438
0.0ThrXaa: 0.0 ± 0.0
Val
8.336ValAla: 8.336 ± 0.624
0.541ValCys: 0.541 ± 0.195
5.846ValAsp: 5.846 ± 0.671
5.413ValGlu: 5.413 ± 0.627
1.894ValPhe: 1.894 ± 0.281
6.333ValGly: 6.333 ± 0.547
1.461ValHis: 1.461 ± 0.247
3.789ValIle: 3.789 ± 0.483
3.789ValLys: 3.789 ± 0.503
4.871ValLeu: 4.871 ± 0.63
1.083ValMet: 1.083 ± 0.205
3.031ValAsn: 3.031 ± 0.408
3.248ValPro: 3.248 ± 0.505
2.111ValGln: 2.111 ± 0.303
5.683ValArg: 5.683 ± 0.579
4.222ValSer: 4.222 ± 0.511
4.547ValThr: 4.547 ± 0.513
4.33ValVal: 4.33 ± 0.487
1.407ValTrp: 1.407 ± 0.242
1.894ValTyr: 1.894 ± 0.308
0.0ValXaa: 0.0 ± 0.0
Trp
2.49TrpAla: 2.49 ± 0.368
0.162TrpCys: 0.162 ± 0.085
1.624TrpAsp: 1.624 ± 0.338
1.516TrpGlu: 1.516 ± 0.285
0.541TrpPhe: 0.541 ± 0.141
1.949TrpGly: 1.949 ± 0.299
0.379TrpHis: 0.379 ± 0.159
0.866TrpIle: 0.866 ± 0.206
0.758TrpLys: 0.758 ± 0.188
1.407TrpLeu: 1.407 ± 0.312
1.028TrpMet: 1.028 ± 0.255
0.595TrpAsn: 0.595 ± 0.168
0.974TrpPro: 0.974 ± 0.289
0.595TrpGln: 0.595 ± 0.161
1.461TrpArg: 1.461 ± 0.288
1.299TrpSer: 1.299 ± 0.241
1.245TrpThr: 1.245 ± 0.274
1.028TrpVal: 1.028 ± 0.233
0.433TrpTrp: 0.433 ± 0.133
0.758TrpTyr: 0.758 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.114TyrAla: 4.114 ± 0.517
0.217TyrCys: 0.217 ± 0.103
3.031TyrAsp: 3.031 ± 0.438
2.003TyrGlu: 2.003 ± 0.287
0.487TyrPhe: 0.487 ± 0.142
2.544TyrGly: 2.544 ± 0.312
0.379TyrHis: 0.379 ± 0.166
1.407TyrIle: 1.407 ± 0.281
1.137TyrLys: 1.137 ± 0.268
1.894TyrLeu: 1.894 ± 0.383
0.704TyrMet: 0.704 ± 0.195
0.812TyrAsn: 0.812 ± 0.219
2.003TyrPro: 2.003 ± 0.339
0.974TyrGln: 0.974 ± 0.209
1.894TyrArg: 1.894 ± 0.331
2.057TyrSer: 2.057 ± 0.415
1.732TyrThr: 1.732 ± 0.259
2.111TyrVal: 2.111 ± 0.304
0.866TyrTrp: 0.866 ± 0.196
1.083TyrTyr: 1.083 ± 0.307
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 120 proteins (18476 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski