Lactobacillus phage LR1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KCT7|A0A3G2KCT7_9CAUD Uncharacterized protein OS=Lactobacillus phage LR1 OX=2419581 PE=4 SV=1
MM1 pKa = 7.8AEE3 pKa = 4.34LDD5 pKa = 3.7ATNDD9 pKa = 3.13IQRR12 pKa = 11.84MQTLLGIDD20 pKa = 4.93LDD22 pKa = 4.37DD23 pKa = 4.64ADD25 pKa = 4.09KK26 pKa = 11.46GRR28 pKa = 11.84VEE30 pKa = 4.4AYY32 pKa = 9.36IVQAKK37 pKa = 7.54QAIMVYY43 pKa = 9.39IRR45 pKa = 11.84KK46 pKa = 9.4YY47 pKa = 10.81LDD49 pKa = 4.03DD50 pKa = 4.39NNFPTEE56 pKa = 3.93LNYY59 pKa = 10.89LVDD62 pKa = 3.91QLTLAKK68 pKa = 10.4YY69 pKa = 10.65NKK71 pKa = 9.3FHH73 pKa = 6.76NEE75 pKa = 3.32GMNSISEE82 pKa = 4.48EE83 pKa = 4.11GLSMTFNSNDD93 pKa = 3.53LKK95 pKa = 11.21DD96 pKa = 3.97YY97 pKa = 11.24LPDD100 pKa = 3.96IEE102 pKa = 5.22AWIDD106 pKa = 3.43STGKK110 pKa = 10.95GDD112 pKa = 3.68LTGNAIGWFF121 pKa = 3.82

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KCQ2|A0A3G2KCQ2_9CAUD Putative tail protein OS=Lactobacillus phage LR1 OX=2419581 PE=4 SV=1
MM1 pKa = 7.41ARR3 pKa = 11.84QRR5 pKa = 11.84VMSEE9 pKa = 3.62QQYY12 pKa = 10.88LNSKK16 pKa = 9.76GVGSAVSDD24 pKa = 3.42YY25 pKa = 11.28MMDD28 pKa = 3.09KK29 pKa = 10.19TVVRR33 pKa = 11.84KK34 pKa = 9.24SAYY37 pKa = 9.43HH38 pKa = 4.51QRR40 pKa = 11.84QDD42 pKa = 3.12EE43 pKa = 4.36RR44 pKa = 11.84SRR46 pKa = 11.84KK47 pKa = 9.29ALKK50 pKa = 10.19QNQDD54 pKa = 2.17QYY56 pKa = 10.09YY57 pKa = 10.35AKK59 pKa = 10.16RR60 pKa = 11.84NQARR64 pKa = 11.84RR65 pKa = 11.84EE66 pKa = 3.77YY67 pKa = 10.25RR68 pKa = 11.84RR69 pKa = 11.84LVSSGKK75 pKa = 9.69VRR77 pKa = 11.84APTQAEE83 pKa = 4.5KK84 pKa = 9.36TWNTAHH90 pKa = 6.87GLSEE94 pKa = 4.05NRR96 pKa = 11.84SVQAARR102 pKa = 11.84RR103 pKa = 11.84VLAKK107 pKa = 10.53HH108 pKa = 5.46GVDD111 pKa = 2.67WKK113 pKa = 8.48TGKK116 pKa = 10.36RR117 pKa = 11.84IAPARR122 pKa = 11.84GRR124 pKa = 11.84GLWPTFTHH132 pKa = 6.58KK133 pKa = 10.72GSSGKK138 pKa = 10.56SSGG141 pKa = 3.42

Molecular weight: 16.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

11590

42

1216

269.5

30.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.42 ± 0.428

0.483 ± 0.119

7.17 ± 0.342

5.384 ± 0.373

3.762 ± 0.22

6.713 ± 0.475

1.795 ± 0.157

5.833 ± 0.291

7.852 ± 0.419

7.17 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.744 ± 0.202

6.721 ± 0.253

3.469 ± 0.283

5.66 ± 0.341

3.753 ± 0.255

6.713 ± 0.387

6.428 ± 0.301

5.781 ± 0.232

1.441 ± 0.174

3.71 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski