Thalassobium sp. R2A62

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Thalassobium; unclassified Thalassobium

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7DAZ9|C7DAZ9_9RHOB Ankyrin repeat protein OS=Thalassobium sp. R2A62 OX=633131 GN=TR2A62_1737 PE=4 SV=1
MM1 pKa = 7.85AIYY4 pKa = 9.83TFTIYY9 pKa = 10.3QATSITTGPITLSGNAVTITVDD31 pKa = 5.39DD32 pKa = 5.08PDD34 pKa = 4.72DD35 pKa = 3.92SQGIGDD41 pKa = 5.37LLTDD45 pKa = 4.32GSGHH49 pKa = 6.2DD50 pKa = 4.09VEE52 pKa = 5.3TGITPSITAVSDD64 pKa = 3.37PAYY67 pKa = 10.63SYY69 pKa = 11.28LIGDD73 pKa = 4.26GLTFNGQWAIDD84 pKa = 3.96GLSGTGWNALILNDD98 pKa = 4.36EE99 pKa = 4.92DD100 pKa = 4.21GSNNASFHH108 pKa = 4.82GTMWLASHH116 pKa = 7.63PDD118 pKa = 3.6NPTALADD125 pKa = 3.8AFISGSNVGIGSRR138 pKa = 11.84TDD140 pKa = 3.0VDD142 pKa = 4.18SVPEE146 pKa = 4.3LVPPAPVEE154 pKa = 4.06TYY156 pKa = 9.57TFHH159 pKa = 6.84TFSQSDD165 pKa = 3.41VGFPQTSAFSKK176 pKa = 10.26DD177 pKa = 3.38GIVFVHH183 pKa = 7.4DD184 pKa = 3.59STNPITTIAVDD195 pKa = 4.25DD196 pKa = 5.0DD197 pKa = 4.61DD198 pKa = 6.32AVLDD202 pKa = 4.33DD203 pKa = 4.2QSTNDD208 pKa = 3.25GQTYY212 pKa = 10.22DD213 pKa = 3.51SSEE216 pKa = 3.77ILQSVNGDD224 pKa = 3.42TSISGNDD231 pKa = 3.02IASFASYY238 pKa = 9.63TVTGDD243 pKa = 3.51DD244 pKa = 3.54GSSFKK249 pKa = 10.95AYY251 pKa = 9.76IIAGSNGTNIRR262 pKa = 11.84DD263 pKa = 3.48IDD265 pKa = 3.9GSALTGFDD273 pKa = 3.28TYY275 pKa = 10.61ITFTQPLIDD284 pKa = 3.86GVEE287 pKa = 4.17YY288 pKa = 9.49TYY290 pKa = 11.28SEE292 pKa = 4.49YY293 pKa = 11.41SPIGQVAYY301 pKa = 10.62ADD303 pKa = 4.02LAEE306 pKa = 4.61QGTFDD311 pKa = 4.48IVGTPPEE318 pKa = 4.31SPEE321 pKa = 4.33AGLSISEE328 pKa = 4.94AIFLGNFMDD337 pKa = 5.24ADD339 pKa = 3.65TSEE342 pKa = 4.66EE343 pKa = 4.08FFPDD347 pKa = 3.67TVNTAPFLGTFGSSSDD363 pKa = 4.26PLWQDD368 pKa = 3.08EE369 pKa = 4.53VLVTYY374 pKa = 10.75EE375 pKa = 4.54DD376 pKa = 3.95VNGDD380 pKa = 3.53GAINTDD386 pKa = 3.52NLSPQYY392 pKa = 10.96LPDD395 pKa = 3.61EE396 pKa = 4.91EE397 pKa = 4.5ISYY400 pKa = 11.18DD401 pKa = 3.97LGSGTQTALVDD412 pKa = 4.35SIVSINLTVTYY423 pKa = 10.03TDD425 pKa = 4.55GSTQSYY431 pKa = 9.28TNALMYY437 pKa = 10.46QDD439 pKa = 5.03DD440 pKa = 4.58AGNMFLVNSDD450 pKa = 3.09WAGTNLNSGSWPQIQSIDD468 pKa = 3.69VTSVTDD474 pKa = 5.08GYY476 pKa = 10.36HH477 pKa = 6.59GAMTQDD483 pKa = 3.89DD484 pKa = 4.38FQSFVCFTRR493 pKa = 11.84GTLIKK498 pKa = 9.04TDD500 pKa = 3.18QGEE503 pKa = 4.16RR504 pKa = 11.84PIEE507 pKa = 3.89EE508 pKa = 4.16LAAGDD513 pKa = 3.69MVLTMDD519 pKa = 5.11HH520 pKa = 7.25GYY522 pKa = 10.36QPIRR526 pKa = 11.84WIGSSKK532 pKa = 10.35RR533 pKa = 11.84AATGDD538 pKa = 3.71LAPILIRR545 pKa = 11.84RR546 pKa = 11.84GALGNDD552 pKa = 3.11RR553 pKa = 11.84DD554 pKa = 4.18LRR556 pKa = 11.84VSPQHH561 pKa = 7.28RR562 pKa = 11.84MLLQGWQAEE571 pKa = 4.29LLFGEE576 pKa = 4.69VEE578 pKa = 4.33VLATAKK584 pKa = 10.68SLLNDD589 pKa = 3.24QTILRR594 pKa = 11.84DD595 pKa = 3.33EE596 pKa = 4.68GGEE599 pKa = 3.86VEE601 pKa = 4.37YY602 pKa = 10.93FHH604 pKa = 6.82MLFDD608 pKa = 3.52THH610 pKa = 6.63EE611 pKa = 4.42IIYY614 pKa = 10.9AEE616 pKa = 4.53GCPSEE621 pKa = 4.83SFHH624 pKa = 7.24PGQQGWKK631 pKa = 10.1ALDD634 pKa = 3.18QATRR638 pKa = 11.84DD639 pKa = 3.74EE640 pKa = 4.55ILTLFPQLVDD650 pKa = 3.52GTLNDD655 pKa = 3.72YY656 pKa = 11.0GPAARR661 pKa = 11.84MSLKK665 pKa = 10.48HH666 pKa = 6.62KK667 pKa = 9.91EE668 pKa = 4.05GKK670 pKa = 10.38LLGSYY675 pKa = 8.52MAGSVV680 pKa = 3.13

Molecular weight: 72.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7D7M6|C7D7M6_9RHOB Phage integrase OS=Thalassobium sp. R2A62 OX=633131 GN=TR2A62_3212 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.91SLSAA44 pKa = 3.86

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3672

0

3672

1040251

33

8645

283.3

30.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.412 ± 0.051

0.937 ± 0.015

6.477 ± 0.038

5.621 ± 0.045

3.904 ± 0.03

8.408 ± 0.058

2.071 ± 0.025

5.595 ± 0.031

3.45 ± 0.037

9.535 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.969 ± 0.027

3.061 ± 0.03

4.643 ± 0.037

3.357 ± 0.024

6.059 ± 0.051

5.498 ± 0.038

5.944 ± 0.042

7.345 ± 0.039

1.389 ± 0.019

2.322 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski