Amino acid dipepetide frequency for Primorskyibacter sp. SS33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.016AlaAla: 21.016 ± 0.242	1.187AlaCys: 1.187 ± 0.039	7.44AlaAsp: 7.44 ± 0.086	9.074AlaGlu: 9.074 ± 0.116	4.546AlaPhe: 4.546 ± 0.066	12.51AlaGly: 12.51 ± 0.157	2.458AlaHis: 2.458 ± 0.045	6.44AlaIle: 6.44 ± 0.084	2.51AlaLys: 2.51 ± 0.063	15.267AlaLeu: 15.267 ± 0.161	4.058AlaMet: 4.058 ± 0.063	2.411AlaAsn: 2.411 ± 0.056	7.501AlaPro: 7.501 ± 0.119	4.171AlaGln: 4.171 ± 0.065	12.284AlaArg: 12.284 ± 0.15	5.291AlaSer: 5.291 ± 0.072	6.441AlaThr: 6.441 ± 0.1	9.084AlaVal: 9.084 ± 0.108	1.731AlaTrp: 1.731 ± 0.047	2.371AlaTyr: 2.371 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	1.215CysAla: 1.215 ± 0.039	0.085CysCys: 0.085 ± 0.01	0.655CysAsp: 0.655 ± 0.029	0.449CysGlu: 0.449 ± 0.022	0.299CysPhe: 0.299 ± 0.016	0.944CysGly: 0.944 ± 0.029	0.251CysHis: 0.251 ± 0.015	0.397CysIle: 0.397 ± 0.018	0.15CysLys: 0.15 ± 0.014	0.852CysLeu: 0.852 ± 0.027	0.149CysMet: 0.149 ± 0.013	0.184CysAsn: 0.184 ± 0.014	0.529CysPro: 0.529 ± 0.026	0.183CysGln: 0.183 ± 0.013	0.672CysArg: 0.672 ± 0.022	0.384CysSer: 0.384 ± 0.02	0.396CysThr: 0.396 ± 0.02	0.585CysVal: 0.585 ± 0.023	0.126CysTrp: 0.126 ± 0.011	0.158CysTyr: 0.158 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	9.04AspAla: 9.04 ± 0.12	0.546AspCys: 0.546 ± 0.025	3.953AspAsp: 3.953 ± 0.09	3.971AspGlu: 3.971 ± 0.076	2.101AspPhe: 2.101 ± 0.046	6.099AspGly: 6.099 ± 0.088	1.334AspHis: 1.334 ± 0.035	2.961AspIle: 2.961 ± 0.053	1.258AspLys: 1.258 ± 0.034	6.938AspLeu: 6.938 ± 0.091	1.604AspMet: 1.604 ± 0.038	1.036AspAsn: 1.036 ± 0.033	4.314AspPro: 4.314 ± 0.063	1.338AspGln: 1.338 ± 0.043	5.889AspArg: 5.889 ± 0.092	1.982AspSer: 1.982 ± 0.048	3.082AspThr: 3.082 ± 0.071	3.74AspVal: 3.74 ± 0.06	1.401AspTrp: 1.401 ± 0.04	1.414AspTyr: 1.414 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	8.701GluAla: 8.701 ± 0.114	0.344GluCys: 0.344 ± 0.019	3.513GluAsp: 3.513 ± 0.066	2.869GluGlu: 2.869 ± 0.076	1.595GluPhe: 1.595 ± 0.042	5.278GluGly: 5.278 ± 0.083	0.986GluHis: 0.986 ± 0.034	3.924GluIle: 3.924 ± 0.065	1.499GluLys: 1.499 ± 0.048	4.959GluLeu: 4.959 ± 0.084	1.717GluMet: 1.717 ± 0.044	1.423GluAsn: 1.423 ± 0.042	2.691GluPro: 2.691 ± 0.066	1.591GluGln: 1.591 ± 0.04	5.136GluArg: 5.136 ± 0.074	2.041GluSer: 2.041 ± 0.052	4.234GluThr: 4.234 ± 0.069	4.183GluVal: 4.183 ± 0.073	0.717GluTrp: 0.717 ± 0.027	0.951GluTyr: 0.951 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.736PheAla: 4.736 ± 0.078	0.387PheCys: 0.387 ± 0.019	2.923PheAsp: 2.923 ± 0.052	2.049PheGlu: 2.049 ± 0.048	1.269PhePhe: 1.269 ± 0.043	3.679PheGly: 3.679 ± 0.066	0.689PheHis: 0.689 ± 0.025	1.401PheIle: 1.401 ± 0.041	0.656PheLys: 0.656 ± 0.028	3.213PheLeu: 3.213 ± 0.059	0.721PheMet: 0.721 ± 0.029	0.804PheAsn: 0.804 ± 0.03	1.593PhePro: 1.593 ± 0.04	0.806PheGln: 0.806 ± 0.031	2.407PheArg: 2.407 ± 0.047	1.777PheSer: 1.777 ± 0.043	1.998PheThr: 1.998 ± 0.049	2.697PheVal: 2.697 ± 0.057	0.574PheTrp: 0.574 ± 0.025	0.825PheTyr: 0.825 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	11.772GlyAla: 11.772 ± 0.129	0.889GlyCys: 0.889 ± 0.03	5.428GlyAsp: 5.428 ± 0.091	4.913GlyGlu: 4.913 ± 0.078	3.744GlyPhe: 3.744 ± 0.057	8.56GlyGly: 8.56 ± 0.143	1.952GlyHis: 1.952 ± 0.045	4.663GlyIle: 4.663 ± 0.083	2.465GlyLys: 2.465 ± 0.052	9.526GlyLeu: 9.526 ± 0.123	2.626GlyMet: 2.626 ± 0.049	1.787GlyAsn: 1.787 ± 0.056	4.423GlyPro: 4.423 ± 0.073	2.789GlyGln: 2.789 ± 0.054	7.786GlyArg: 7.786 ± 0.084	3.923GlySer: 3.923 ± 0.068	4.958GlyThr: 4.958 ± 0.077	6.374GlyVal: 6.374 ± 0.084	1.64GlyTrp: 1.64 ± 0.043	2.055GlyTyr: 2.055 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.498HisAla: 2.498 ± 0.053	0.222HisCys: 0.222 ± 0.014	1.402HisAsp: 1.402 ± 0.039	1.103HisGlu: 1.103 ± 0.03	0.707HisPhe: 0.707 ± 0.03	1.919HisGly: 1.919 ± 0.047	0.54HisHis: 0.54 ± 0.024	0.863HisIle: 0.863 ± 0.025	0.362HisLys: 0.362 ± 0.019	1.931HisLeu: 1.931 ± 0.052	0.471HisMet: 0.471 ± 0.025	0.35HisAsn: 0.35 ± 0.018	1.369HisPro: 1.369 ± 0.035	0.423HisGln: 0.423 ± 0.02	1.581HisArg: 1.581 ± 0.039	0.728HisSer: 0.728 ± 0.026	0.743HisThr: 0.743 ± 0.03	1.587HisVal: 1.587 ± 0.045	0.335HisTrp: 0.335 ± 0.018	0.491HisTyr: 0.491 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	7.72IleAla: 7.72 ± 0.102	0.566IleCys: 0.566 ± 0.022	3.616IleAsp: 3.616 ± 0.063	3.626IleGlu: 3.626 ± 0.057	1.606IlePhe: 1.606 ± 0.041	4.846IleGly: 4.846 ± 0.08	0.864IleHis: 0.864 ± 0.025	1.468IleIle: 1.468 ± 0.046	0.904IleLys: 0.904 ± 0.034	4.732IleLeu: 4.732 ± 0.083	0.892IleMet: 0.892 ± 0.03	0.963IleAsn: 0.963 ± 0.035	2.198IlePro: 2.198 ± 0.046	0.889IleGln: 0.889 ± 0.032	3.335IleArg: 3.335 ± 0.059	2.339IleSer: 2.339 ± 0.054	2.256IleThr: 2.256 ± 0.05	4.278IleVal: 4.278 ± 0.072	0.583IleTrp: 0.583 ± 0.024	1.002IleTyr: 1.002 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	2.724LysAla: 2.724 ± 0.059	0.115LysCys: 0.115 ± 0.011	1.212LysAsp: 1.212 ± 0.037	1.004LysGlu: 1.004 ± 0.044	0.606LysPhe: 0.606 ± 0.023	2.057LysGly: 2.057 ± 0.053	0.437LysHis: 0.437 ± 0.02	1.16LysIle: 1.16 ± 0.035	0.782LysLys: 0.782 ± 0.035	2.197LysLeu: 2.197 ± 0.056	0.55LysMet: 0.55 ± 0.023	0.502LysAsn: 0.502 ± 0.024	1.369LysPro: 1.369 ± 0.043	0.564LysGln: 0.564 ± 0.028	1.837LysArg: 1.837 ± 0.049	1.289LysSer: 1.289 ± 0.036	1.42LysThr: 1.42 ± 0.04	1.613LysVal: 1.613 ± 0.047	0.268LysTrp: 0.268 ± 0.016	0.445LysTyr: 0.445 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	14.447LeuAla: 14.447 ± 0.164	1.024LeuCys: 1.024 ± 0.032	6.862LeuAsp: 6.862 ± 0.094	4.965LeuGlu: 4.965 ± 0.073	3.395LeuPhe: 3.395 ± 0.062	9.276LeuGly: 9.276 ± 0.109	1.866LeuHis: 1.866 ± 0.049	4.638LeuIle: 4.638 ± 0.071	2.359LeuLys: 2.359 ± 0.056	8.574LeuLeu: 8.574 ± 0.123	2.327LeuMet: 2.327 ± 0.053	2.189LeuAsn: 2.189 ± 0.051	5.783LeuPro: 5.783 ± 0.088	2.217LeuGln: 2.217 ± 0.042	8.177LeuArg: 8.177 ± 0.104	6.24LeuSer: 6.24 ± 0.093	5.591LeuThr: 5.591 ± 0.074	7.183LeuVal: 7.183 ± 0.081	1.388LeuTrp: 1.388 ± 0.043	1.851LeuTyr: 1.851 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	3.549MetAla: 3.549 ± 0.069	0.153MetCys: 0.153 ± 0.013	1.284MetAsp: 1.284 ± 0.034	1.201MetGlu: 1.201 ± 0.037	0.719MetPhe: 0.719 ± 0.028	2.265MetGly: 2.265 ± 0.058	0.398MetHis: 0.398 ± 0.02	1.331MetIle: 1.331 ± 0.035	0.753MetLys: 0.753 ± 0.027	2.435MetLeu: 2.435 ± 0.051	0.648MetMet: 0.648 ± 0.027	0.684MetAsn: 0.684 ± 0.025	1.561MetPro: 1.561 ± 0.041	0.793MetGln: 0.793 ± 0.03	2.081MetArg: 2.081 ± 0.044	1.574MetSer: 1.574 ± 0.04	2.062MetThr: 2.062 ± 0.042	1.675MetVal: 1.675 ± 0.044	0.213MetTrp: 0.213 ± 0.014	0.265MetTyr: 0.265 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.737AsnAla: 2.737 ± 0.055	0.201AsnCys: 0.201 ± 0.013	1.204AsnAsp: 1.204 ± 0.046	0.991AsnGlu: 0.991 ± 0.035	0.79AsnPhe: 0.79 ± 0.03	1.886AsnGly: 1.886 ± 0.046	0.425AsnHis: 0.425 ± 0.021	1.051AsnIle: 1.051 ± 0.035	0.402AsnLys: 0.402 ± 0.023	2.114AsnLeu: 2.114 ± 0.049	0.52AsnMet: 0.52 ± 0.025	0.471AsnAsn: 0.471 ± 0.025	1.578AsnPro: 1.578 ± 0.038	0.499AsnGln: 0.499 ± 0.023	1.642AsnArg: 1.642 ± 0.045	0.837AsnSer: 0.837 ± 0.029	0.984AsnThr: 0.984 ± 0.031	1.553AsnVal: 1.553 ± 0.041	0.38AsnTrp: 0.38 ± 0.02	0.462AsnTyr: 0.462 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	7.173ProAla: 7.173 ± 0.102	0.391ProCys: 0.391 ± 0.02	4.598ProAsp: 4.598 ± 0.064	4.668ProGlu: 4.668 ± 0.082	2.003ProPhe: 2.003 ± 0.045	5.417ProGly: 5.417 ± 0.082	1.112ProHis: 1.112 ± 0.037	2.116ProIle: 2.116 ± 0.051	1.129ProLys: 1.129 ± 0.036	4.821ProLeu: 4.821 ± 0.074	1.275ProMet: 1.275 ± 0.038	1.045ProAsn: 1.045 ± 0.035	3.017ProPro: 3.017 ± 0.066	1.522ProGln: 1.522 ± 0.043	4.03ProArg: 4.03 ± 0.076	2.387ProSer: 2.387 ± 0.048	2.306ProThr: 2.306 ± 0.047	4.516ProVal: 4.516 ± 0.071	0.797ProTrp: 0.797 ± 0.032	1.082ProTyr: 1.082 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	3.641GlnAla: 3.641 ± 0.062	0.166GlnCys: 0.166 ± 0.014	1.57GlnAsp: 1.57 ± 0.042	1.207GlnGlu: 1.207 ± 0.038	0.819GlnPhe: 0.819 ± 0.031	2.471GlnGly: 2.471 ± 0.05	0.458GlnHis: 0.458 ± 0.022	1.615GlnIle: 1.615 ± 0.041	0.716GlnLys: 0.716 ± 0.029	2.17GlnLeu: 2.17 ± 0.047	0.813GlnMet: 0.813 ± 0.031	0.658GlnAsn: 0.658 ± 0.025	1.401GlnPro: 1.401 ± 0.036	0.844GlnGln: 0.844 ± 0.033	1.96GlnArg: 1.96 ± 0.052	1.448GlnSer: 1.448 ± 0.041	1.494GlnThr: 1.494 ± 0.036	1.974GlnVal: 1.974 ± 0.045	0.347GlnTrp: 0.347 ± 0.016	0.46GlnTyr: 0.46 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	11.166ArgAla: 11.166 ± 0.13	0.589ArgCys: 0.589 ± 0.024	5.669ArgAsp: 5.669 ± 0.079	4.402ArgGlu: 4.402 ± 0.078	2.991ArgPhe: 2.991 ± 0.063	6.019ArgGly: 6.019 ± 0.085	1.851ArgHis: 1.851 ± 0.043	4.675ArgIle: 4.675 ± 0.074	1.937ArgLys: 1.937 ± 0.043	8.918ArgLeu: 8.918 ± 0.111	2.221ArgMet: 2.221 ± 0.046	1.698ArgAsn: 1.698 ± 0.045	4.542ArgPro: 4.542 ± 0.085	2.309ArgGln: 2.309 ± 0.054	6.847ArgArg: 6.847 ± 0.106	3.323ArgSer: 3.323 ± 0.062	3.456ArgThr: 3.456 ± 0.061	5.611ArgVal: 5.611 ± 0.077	1.047ArgTrp: 1.047 ± 0.032	1.502ArgTyr: 1.502 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	5.356SerAla: 5.356 ± 0.071	0.394SerCys: 0.394 ± 0.023	3.046SerAsp: 3.046 ± 0.051	2.439SerGlu: 2.439 ± 0.05	2.033SerPhe: 2.033 ± 0.046	5.031SerGly: 5.031 ± 0.089	0.914SerHis: 0.914 ± 0.034	2.13SerIle: 2.13 ± 0.049	0.98SerLys: 0.98 ± 0.031	4.361SerLeu: 4.361 ± 0.075	1.156SerMet: 1.156 ± 0.029	1.068SerAsn: 1.068 ± 0.033	2.448SerPro: 2.448 ± 0.053	1.229SerGln: 1.229 ± 0.033	3.448SerArg: 3.448 ± 0.063	2.098SerSer: 2.098 ± 0.046	2.22SerThr: 2.22 ± 0.054	3.453SerVal: 3.453 ± 0.059	0.614SerTrp: 0.614 ± 0.025	1.099SerTyr: 1.099 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	6.511ThrAla: 6.511 ± 0.081	0.434ThrCys: 0.434 ± 0.024	3.202ThrAsp: 3.202 ± 0.062	2.957ThrGlu: 2.957 ± 0.056	1.81ThrPhe: 1.81 ± 0.045	5.66ThrGly: 5.66 ± 0.09	1.015ThrHis: 1.015 ± 0.031	2.395ThrIle: 2.395 ± 0.05	0.92ThrLys: 0.92 ± 0.035	5.993ThrLeu: 5.993 ± 0.077	1.176ThrMet: 1.176 ± 0.036	0.993ThrAsn: 0.993 ± 0.031	3.463ThrPro: 3.463 ± 0.058	1.29ThrGln: 1.29 ± 0.036	4.062ThrArg: 4.062 ± 0.062	2.136ThrSer: 2.136 ± 0.047	2.522ThrThr: 2.522 ± 0.056	4.229ThrVal: 4.229 ± 0.056	0.641ThrTrp: 0.641 ± 0.026	1.038ThrTyr: 1.038 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	9.806ValAla: 9.806 ± 0.104	0.643ValCys: 0.643 ± 0.024	4.114ValAsp: 4.114 ± 0.062	4.714ValGlu: 4.714 ± 0.075	2.845ValPhe: 2.845 ± 0.055	5.348ValGly: 5.348 ± 0.071	1.291ValHis: 1.291 ± 0.041	3.918ValIle: 3.918 ± 0.076	1.625ValLys: 1.625 ± 0.045	7.563ValLeu: 7.563 ± 0.1	1.96ValMet: 1.96 ± 0.051	1.631ValAsn: 1.631 ± 0.044	3.833ValPro: 3.833 ± 0.061	1.778ValGln: 1.778 ± 0.048	4.637ValArg: 4.637 ± 0.071	3.952ValSer: 3.952 ± 0.067	4.529ValThr: 4.529 ± 0.072	5.621ValVal: 5.621 ± 0.076	1.055ValTrp: 1.055 ± 0.03	1.342ValTyr: 1.342 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.526TrpAla: 1.526 ± 0.035	0.137TrpCys: 0.137 ± 0.013	0.797TrpAsp: 0.797 ± 0.025	0.649TrpGlu: 0.649 ± 0.025	0.541TrpPhe: 0.541 ± 0.022	1.063TrpGly: 1.063 ± 0.035	0.321TrpHis: 0.321 ± 0.018	0.779TrpIle: 0.779 ± 0.026	0.352TrpLys: 0.352 ± 0.018	1.801TrpLeu: 1.801 ± 0.055	0.367TrpMet: 0.367 ± 0.019	0.379TrpAsn: 0.379 ± 0.019	0.802TrpPro: 0.802 ± 0.028	0.505TrpGln: 0.505 ± 0.022	1.387TrpArg: 1.387 ± 0.044	0.845TrpSer: 0.845 ± 0.031	0.807TrpThr: 0.807 ± 0.027	0.882TrpVal: 0.882 ± 0.031	0.248TrpTrp: 0.248 ± 0.018	0.246TrpTyr: 0.246 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.459TyrAla: 2.459 ± 0.052	0.193TyrCys: 0.193 ± 0.014	1.512TyrAsp: 1.512 ± 0.043	1.103TyrGlu: 1.103 ± 0.036	0.785TyrPhe: 0.785 ± 0.03	1.942TyrGly: 1.942 ± 0.042	0.462TyrHis: 0.462 ± 0.024	0.758TyrIle: 0.758 ± 0.031	0.389TyrLys: 0.389 ± 0.02	1.992TyrLeu: 1.992 ± 0.041	0.394TyrMet: 0.394 ± 0.019	0.446TyrAsn: 0.446 ± 0.023	0.988TyrPro: 0.988 ± 0.03	0.494TyrGln: 0.494 ± 0.025	1.579TyrArg: 1.579 ± 0.044	0.924TyrSer: 0.924 ± 0.028	0.946TyrThr: 0.946 ± 0.031	1.366TyrVal: 1.366 ± 0.036	0.329TyrTrp: 0.329 ± 0.017	0.462TyrTyr: 0.462 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3259 proteins (1031760 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---