Pantoea ananatis (strain LMG 20103)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Pantoea; Pantoea ananatis

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4241 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4GE23|D4GE23_PANAM 3-deoxy-D-manno-octulosonic acid transferase OS=Pantoea ananatis (strain LMG 20103) OX=706191 GN=kdtA PE=3 SV=1
MM1 pKa = 7.59SEE3 pKa = 4.28HH4 pKa = 6.18NTNEE8 pKa = 3.81MQFQIQRR15 pKa = 11.84VYY17 pKa = 10.34TKK19 pKa = 10.06DD20 pKa = 3.11ISFEE24 pKa = 4.19APNAPQVFQKK34 pKa = 10.33EE35 pKa = 4.17WEE37 pKa = 4.2PDD39 pKa = 3.28VKK41 pKa = 11.0LDD43 pKa = 4.46LDD45 pKa = 4.05TASSQLADD53 pKa = 3.38EE54 pKa = 4.72VYY56 pKa = 10.38EE57 pKa = 4.17VVLRR61 pKa = 11.84VTVTATVGEE70 pKa = 4.37DD71 pKa = 3.04TAFLCEE77 pKa = 4.07VQQAGIFTIGGIDD90 pKa = 3.73GTQMAHH96 pKa = 7.07CLGAYY101 pKa = 9.15CPNILFPYY109 pKa = 9.96ARR111 pKa = 11.84EE112 pKa = 4.54CITSLVSRR120 pKa = 11.84GTFPQLNLAPVNFDD134 pKa = 3.64ALFMNYY140 pKa = 9.34LQQQQGEE147 pKa = 4.29EE148 pKa = 4.36GAAQHH153 pKa = 6.4QDD155 pKa = 2.75AA156 pKa = 4.88

Molecular weight: 17.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4GGG8|D4GGG8_PANAM MipA OS=Pantoea ananatis (strain LMG 20103) OX=706191 GN=mipA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4241

0

4241

1372081

26

4179

323.5

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.103 ± 0.043

1.065 ± 0.012

5.18 ± 0.03

5.269 ± 0.038

3.863 ± 0.027

7.364 ± 0.038

2.372 ± 0.024

5.573 ± 0.031

3.896 ± 0.031

11.026 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.821 ± 0.019

3.639 ± 0.033

4.477 ± 0.024

4.806 ± 0.034

5.898 ± 0.038

6.165 ± 0.036

5.318 ± 0.043

7.025 ± 0.031

1.513 ± 0.017

2.626 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski