Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Fumigati; Aspergillus fischeri

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10409 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1DF87|A1DF87_NEOFI AMP dependent CoA ligase OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=NFIA_079860 PE=4 SV=1
MM1 pKa = 7.4TKK3 pKa = 8.47MHH5 pKa = 6.33NVKK8 pKa = 10.27KK9 pKa = 10.65LSALLCLLLAVGPTAVLSAPIADD32 pKa = 3.8VEE34 pKa = 4.6SLAKK38 pKa = 10.32RR39 pKa = 11.84QATHH43 pKa = 6.6SNTDD47 pKa = 3.12AASVVGAGIPDD58 pKa = 3.77GPSFNQGDD66 pKa = 4.19FFSTSSDD73 pKa = 2.93EE74 pKa = 5.87HH75 pKa = 8.18RR76 pKa = 11.84DD77 pKa = 3.45EE78 pKa = 6.34DD79 pKa = 4.84CDD81 pKa = 3.82EE82 pKa = 5.56DD83 pKa = 5.37CDD85 pKa = 4.0EE86 pKa = 6.11DD87 pKa = 5.37CDD89 pKa = 4.0EE90 pKa = 6.11DD91 pKa = 5.37CDD93 pKa = 4.0EE94 pKa = 6.11DD95 pKa = 5.37CDD97 pKa = 4.0EE98 pKa = 6.11DD99 pKa = 5.37CDD101 pKa = 4.0EE102 pKa = 6.11DD103 pKa = 5.37CDD105 pKa = 4.0EE106 pKa = 6.11DD107 pKa = 5.37CDD109 pKa = 4.03EE110 pKa = 5.86DD111 pKa = 5.37CDD113 pKa = 4.47EE114 pKa = 6.55DD115 pKa = 4.71EE116 pKa = 6.33DD117 pKa = 5.6NDD119 pKa = 4.99DD120 pKa = 4.42SPSSPGSSSSSNTGAASFMNAGIPDD145 pKa = 4.21GPSFNQGDD153 pKa = 4.13FFSTGNDD160 pKa = 2.95EE161 pKa = 4.52HH162 pKa = 8.38RR163 pKa = 11.84DD164 pKa = 3.61VGDD167 pKa = 3.98GDD169 pKa = 4.14NGSQSGFSTSTDD181 pKa = 2.98AGDD184 pKa = 3.39FATFGIPDD192 pKa = 3.99GPSATFGKK200 pKa = 9.99FFHH203 pKa = 7.09HH204 pKa = 7.68DD205 pKa = 3.51YY206 pKa = 11.33EE207 pKa = 4.69EE208 pKa = 4.31THH210 pKa = 6.21SEE212 pKa = 4.14QPEE215 pKa = 4.4TTHH218 pKa = 6.83VPEE221 pKa = 4.76PTPPTPPVEE230 pKa = 4.75TPTSTPPAHH239 pKa = 6.43TPAPAPPTSAPPAPPAPPAPPAPPAPPAPPAPPAPPAPAPAPPVPAPPPAPPAQTSEE296 pKa = 4.49VPAPAPPPAPPAPAPEE312 pKa = 4.63APVAAPPAPAPAPPADD328 pKa = 4.09HH329 pKa = 7.12SCTCSS334 pKa = 2.93

Molecular weight: 33.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A1CX31|A1CX31_NEOFI Uncharacterized protein OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=NFIA_106710 PE=4 SV=1
MM1 pKa = 7.1QRR3 pKa = 11.84ATPASQPTGAGTAIQAGNASTATEE27 pKa = 3.96TAAPNMVTTRR37 pKa = 11.84SSPRR41 pKa = 11.84GNAAPARR48 pKa = 11.84GAPSGRR54 pKa = 11.84RR55 pKa = 11.84AHH57 pKa = 5.67GRR59 pKa = 11.84ARR61 pKa = 11.84RR62 pKa = 11.84GHH64 pKa = 5.13VASTRR69 pKa = 11.84GASNAATAPRR79 pKa = 11.84NRR81 pKa = 11.84PAGRR85 pKa = 11.84GRR87 pKa = 11.84AASRR91 pKa = 11.84AGRR94 pKa = 11.84SGGAARR100 pKa = 11.84GQHH103 pKa = 6.31LLNHH107 pKa = 6.46LTQQ110 pKa = 4.07

Molecular weight: 10.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10409

0

10409

5077491

30

6269

487.8

54.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.678 ± 0.018

1.248 ± 0.009

5.618 ± 0.015

6.21 ± 0.023

3.727 ± 0.013

6.743 ± 0.021

2.402 ± 0.01

4.902 ± 0.017

4.637 ± 0.017

9.114 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.135 ± 0.008

3.597 ± 0.012

5.97 ± 0.025

4.074 ± 0.017

6.238 ± 0.02

8.286 ± 0.026

5.866 ± 0.017

6.232 ± 0.015

1.47 ± 0.009

2.843 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski